Biomolecules & biomedicine最新文献

{"title":"Chronic inorganic arsenic exposure impairs locomotor performance and socio-affective behavior in zebrafish.","authors":"Cătălina Ionescu, Petru Fabian Lungu, Ionut-Alexandru Chelaru, Mircea Nicusor Nicoara, Gabriel Plavan, Ioana-Miruna Balmus, Viorica Rarinca, Alin Ciobica, Bogdan Novac, Diana Gheban, Daniela-Ivona Tomița, Alexandra Savuca, Bogdan Gurzu","doi":"10.17305/bb.2026.14094","DOIUrl":"https://doi.org/10.17305/bb.2026.14094","url":null,"abstract":"<p><p>Inorganic arsenic (iAs) is a prevalent and highly toxic environmental contaminant, yet its neurotoxic effects on aquatic organisms are not well characterized. This study examines whether chronic exposure to environmentally relevant concentrations of iAs affects locomotor performance and socio-affective behavior in adult zebrafish (Danio rerio). Zebrafish were exposed to 10, 50, or 300 µg/L of iAs for 21 consecutive days, with behavioral assessments conducted on Days 0, 7, 14, and 21 using standardized locomotor and social preference tasks. Chronic exposure to iAs resulted in time- and dose-dependent neurobehavioral alterations. The most pronounced locomotor effects occurred at concentrations of 50 and 300 µg/L, revealing significant changes in swimming velocity, immobility duration, and counter-clockwise rotations, which indicate disrupted swimming patterns and altered decision-making behavior. Additionally, in the social task, chronic iAs exposure diminished social preference, evidenced by reduced time spent in the social arm and broader shifts in maze-arm exploration, highlighting a dose-dependent disruption of socio-affective behavior. Overall, chronic exposure to environmentally relevant concentrations of iAs is linked to neurobehavioral impairments that affect locomotion, exploratory and decision-making behavior, and social interactions in zebrafish. These findings underscore the neurotoxic potential of arsenic in aquatic organisms and emphasize surface-water contamination as a significant ecological and public health concern.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147647458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulatory B cells and SIVA1-LAG3 immune suppression predict poor prognosis in prostate cancer.","authors":"Weicheng Tian, Yize Li, Jinchuang Li, Chenyu Liang, Shanghua Cai, Biyan Wen, Zeheng Tan, Zhenjie Wu, Yulin Deng, Zige Liu, Yongding Wu, Haiyin Xiao, Weide Zhong, Huichan He, Chitin Hon, Jianheng Ye","doi":"10.17305/bb.2026.14056","DOIUrl":"https://doi.org/10.17305/bb.2026.14056","url":null,"abstract":"<p><p>Prostate cancer (PCa) is among the most prevalent malignancies affecting men globally. The immunosuppressive characteristics of the tumor microenvironment significantly hinder the effectiveness of immunotherapeutic strategies. B cells have a dual role in immune responses, with regulatory B cells (Bregs) promoting immune tolerance through the secretion of immunosuppressive cytokines, facilitating tumor immune evasion. However, the specific role of Bregs in prostate cancer remains inadequately understood. In this study, we systematically identified Breg subpopulations in prostate cancer using single-cell RNA sequencing (scRNA-seq), multi-omics analysis, and machine learning techniques, while also investigating their association with clinical prognosis. Our analysis revealed that increased infiltration of Bregs is strongly correlated with shorter biochemical recurrence-free survival (BCRFS). Additionally, higher expression levels of SIVA1 apoptosis-inducing factor (SIVA1) were positively associated with the immune checkpoint lymphocyte-activation gene 3 (LAG3). Utilizing Breg-related prognostic genes (BRPGs), we developed a novel model for predicting BCRFS, which we validated across multiple independent cohorts. Further immunohistochemical analysis confirmed the positive correlation between elevated SIVA1 expression and poor prognosis, as well as its association with LAG3. Collectively, these findings provide new insights into the immune microenvironment of prostate cancer and suggest that Breg-related characteristics and the SIVA1-LAG3 relationship in tumor cells may represent promising therapeutic targets for future research.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147647418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Claudin 18.2, IDH1, and neutrophil-to-lymphocyte ratio as prognostic biomarkers in unresectable pancreatic ductal adenocarcinoma receiving first-line chemotherapy.","authors":"Ebru Karcı, Ferhat Ozden, Elif Kuzucular, Ozgur Acikgoz, Ömer Fatih Ölmez, Ozcan Yildiz, Hafize Uzun, Ahmet Bilici","doi":"10.17305/bb.2026.14093","DOIUrl":"https://doi.org/10.17305/bb.2026.14093","url":null,"abstract":"<p><p>Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with few effective treatment options. This study investigates the prognostic significance of claudin 18.2 (CLDN18.2), isocitrate dehydrogenase-1 (IDH1) expression, and the neutrophil-to-lymphocyte ratio (NLR) in patients with unresectable PDAC undergoing first-line chemotherapy. This single-center retrospective analysis utilized prospectively collected data from 72 patients treated with FOLFIRINOX (59.7%) or gemcitabine plus nab-paclitaxel (40.3%). CLDN18.2 and IDH1 expression levels were evaluated through immunohistochemistry, while NLR was calculated from pretreatment blood counts. Survival analyses were conducted using univariable and multivariable Cox regression models. CLDN18.2 positivity was found in 18.1% of patients, and IDH1 positivity was observed in 2.8%. Additionally, an elevated NLR (>3.22) was present in 56.9% of patients. The median progression-free survival (PFS) and overall survival (OS) were 7.1 months (95% CI: 4.2-9.9) and 9.7 months (95% CI: 8.5-10.8), respectively. In the multivariable analysis, CLDN18.2 expression, IDH1 expression, and NLR were significantly associated with OS, whereas no consistent associations were found for PFS. Effect estimates include corresponding 95% confidence intervals. These findings indicate the potential prognostic value of integrating molecular and systemic biomarkers in unresectable PDAC. However, due to the retrospective design and limited sample size, these results should be regarded as hypothesis-generating and necessitate validation in larger, independent cohorts prior to clinical implementation.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxytocin modulates social preference and anxiety-like locomotor behavior in albino and non-albino zebrafish.","authors":"Ana Maria Danila, Alexandra Savuca, Mircea-Nicusor Nicoara, Alin Stelian Ciobica, Bogdan Novac, Stefan Chiriac, Daniela Tomita, Ovidiu-Dumitru Ilie","doi":"10.17305/bb.2026.13819","DOIUrl":"https://doi.org/10.17305/bb.2026.13819","url":null,"abstract":"<p><p>Socio-emotional and exploratory behaviors in zebrafish (Danio rerio) are shaped by conserved neuromodulators such as oxytocin (OXT), yet phenotypic variation (e.g., albinism) may modify sensitivity to OXT-dependent regulation. Here, we tested whether pigmentation phenotype alters behavioral responses to acute OXT across structured social and anxiogenic contexts. Adult albino (A; n = 30) and non-albino (N; n = 30) zebrafish (total n = 60; equal allocation across 6 groups, n = 10/group) were exposed to OXT by immersion (33.2 ng/mL, 15 min) under a 2 × 3 factorial design (Phenotype × Dosing regimen: Vehicle; OXT single exposure assessed at 24 h; OXT repeated exposure assessed at 48 h) and evaluated using the social preference test (SPT), social interaction (SI) test, and novel tank test (NTT). In the SPT, decision-zone occupancy and mean distance to the non-social arm were robustly modulated by dosing (p < 0.0001; p = 0.007) without phenotype × dosing interactions; locomotor outputs (swimming distance, p = 0.040; velocity, p = 0.041; counterclockwise rotations, p = 0.006) also showed dosing effects only, supporting a dissociation between social allocation and global motor output. In the SI test, stimulus-zone occupancy showed a significant main effect of phenotype (p = 0.009), independent of dosing, and locomotor indices similarly exhibited phenotype effects without interaction (swimming distance and velocity, p = 0.017; mobile-state duration, p = 0.002). In contrast, the NTT revealed phenotype-dependent locomotor regulation: highly mobile duration showed a significant interaction (p = 0.002) with strong main effects of phenotype and dosing (both p < 0.001), while immobile duration displayed an interaction (p = 0.015) and dosing effect (p = 0.005), driven primarily by the non-albino phenotype; clockwise rotation (p = 0.018) and active-state duration (p = 0.030) further indicated phenotype-related differences. Collectively, OXT exerts context- and dosing-regimen-dependent modulation in zebrafish, with social allocation primarily dosing-driven and largely phenotype-independent, whereas anxiety-related locomotor dynamics show marked phenotype specificity.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural MRI phenotyping in Alzheimer's disease: Comparison of visual rating scales, volumetry, and cortical thickness in a Serbian single-centre cohort.","authors":"Aleksandra Aracki-Trenkic, Milica Živanović, Vuk Milošević, Jelena Bašić, Dunja Radovanović, Mrina Malobabić","doi":"10.17305/bb.2026.13974","DOIUrl":"https://doi.org/10.17305/bb.2026.13974","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is characterized by a heterogeneous clinical course, and magnetic resonance imaging (MRI)-based phenotyping has increasingly been utilized to elucidate this variability. The literature recognizes four predominant MRI phenotypes: typical, hippocampal-sparing, limbic-predominant, and minimal-atrophy. However, the compatibility of various MRI phenotyping methods remains insufficiently defined. This study aimed to assess the concordance between MRI phenotyping methods within a Serbian cohort consisting of 40 subjects. Four MRI phenotyping approaches were employed: scale-based, adjusted scale-based, volume-based, and thickness-based. The scale-based method exhibited moderate agreement with the adjusted scale-based approach and high concordance with volumetric methods. In contrast, the relationship between scale- and thickness-based phenotyping was less clear. The lack of significant agreement with demographic variables, along with the observed differences across clinical dementia rating (CDR) domains, further underscored the clinical heterogeneity among phenotypes. Overall, these findings suggest that visual scale-based MRI phenotyping may serve as a practical approach in resource-limited clinical settings where advanced methods are unavailable. However, the results must be interpreted with caution and require validation in larger independent cohorts. Further research is necessary to clarify the relationship between scale- and thickness-based phenotyping across different disease stages and to investigate discrepancies in demographic, apolipoprotein E (APOE)-related, and clinical phenotype patterns in this Serbian sample compared to other populations.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-dose metformin in the brain: A focused review of its novel mechanism of glucose lowering in type 2 diabetes.","authors":"Zain Ahmad, Saghir Akhtar","doi":"10.17305/bb.2026.14097","DOIUrl":"https://doi.org/10.17305/bb.2026.14097","url":null,"abstract":"<p><p>Metformin remains the first-line pharmacological therapy for type 2 diabetes mellitus (T2DM). While its glucose-lowering effects have primarily been attributed to peripheral actions, evidence indicates that it also crosses the blood-brain barrier (BBB) and can exert anti-diabetic effects from within the central nervous system (CNS). This focused review discusses metformin's central actions and how they might integrate with established peripheral mechanisms of glucose-lowering. We synthesized recent mechanistic studies conducted in murine models using systemic and intracerebroventricular metformin administration, brain-specific genetic loss- and gain-of-function approaches, neuronal activation mapping, electrophysiology, and pharmacological interrogation of autonomic and metabolic pathways. The evidence reviewed indicates that clinically relevant low concentrations of metformin in the brain engage a ventromedial hypothalamus (VMH)-steroidogenic factor 1 (SF1) neuron-Ras-proximate-1 (Rap1) signaling axis, which is essential for its glucose lowering action. Notably, metformin inhibits Rap1 in VMH SF1 neurons to reduce hyperglycemia. This central mechanism appears specific to metformin and is not shared by other approved antidiabetic agents. Current data also imply that the CNS effects of metformin operate in conjunction with peripheral pathways including hepatic AMP-activated protein kinase (AMPK) signaling and gut-derived glucagon-like peptide-1 (GLP-1) secretion, to produce its overall metabolic effect. Collectively, these findings support a model in which metformin lowers glucose through an orchestrated integration of a central Rap-1 dependent mechanism and peripheral metabolic actions. Additionally, the recognition of a central component to metformin's pharmacology expands the mechanistic framework of this cornerstone therapy and may inform future therapeutic strategies targeting integrated brain-metabolic pathways in T2DM.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Higher serum PFDA is associated with increased non-melanoma skin cancer odds in NHANES 2003-2018.","authors":"Sundus Siddiqui, Hiba Siddiqui, Karim Nagi, Mohammed Imad Malki","doi":"10.17305/bb.2026.13621","DOIUrl":"https://doi.org/10.17305/bb.2026.13621","url":null,"abstract":"<p><p>Per- and polyfluoroalkyl substances (PFAS) are environmentally persistent chemicals commonly employed in heat- and water-resistant applications. Experimental evidence indicates a biological plausibility for PFAS-related carcinogenic effects through mechanisms such as oxidative stress and immunomodulation; however, epidemiological evidence regarding skin cancers remains limited. This study aimed to investigate the association between serum concentrations of three understudied PFAS-perfluorodecanoic acid (PFDA), perfluoroundecanoic acid (PFUnDA), and 2-(N-methyl-perfluorooctane sulfonamido) acetic acid (2-(N-methyl-PFOSA) acetate)-and the incidence of non-melanoma skin cancer (NMSC) and melanoma among U.S. adults. We conducted a cross-sectional analysis utilizing data from eight NHANES cycles (2003-2018), which included 5,934 adults aged 20 years and older with complete data. Skin cancer outcomes were determined based on self-reported physician diagnoses, while PFAS concentrations were assessed in serum samples. We employed multivariable logistic regression, adjusting for age, sex, race/ethnicity, body mass index, smoking status, and NHANES cycle. PFAS exposure was evaluated using tertiles, detectable/undetectable status, and log-transformed continuous measures. Additionally, exploratory age- and sex-stratified analyses were conducted using Firth penalized logistic regression, and multiple imputation was applied to address potential selection bias due to missing covariates. Compared to the lowest tertile, PFDA in the second tertile was associated with an increased likelihood of NMSC (adjusted odds ratio [aOR] 1.73, 95% uncertainty interval [UI] 1.01-2.89; p=0.048), although no clear dose-response trend was observed across tertiles. Among adults aged 60 years and older, PFDA in the second tertile was linked to higher odds of NMSC (aOR 2.29, 95% UI 1.29-4.05; p=0.004). Associations for PFUnDA and 2-(N-methyl-PFOSA) acetate were generally inconsistent across exposure metrics, and analyses for melanoma did not reveal a definitive association. These findings suggest a potential link between higher PFDA exposure and NMSC, particularly in older adults, underscoring the necessity for ongoing PFAS monitoring and well-designed prospective studies to elucidate the temporal relationship and causality.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic nutritional index predicts 1-year hospitalization and mortality in outpatients with cirrhosis.","authors":"Ayşe Karaduru Avcı, Zeynep Gök Sargın, Fatih Sargın","doi":"10.17305/bb.2026.14035","DOIUrl":"10.17305/bb.2026.14035","url":null,"abstract":"<p><p>Malnutrition is prevalent in patients with cirrhosis and is linked to negative clinical outcomes. However, research on the prognostic nutritional index (PNI) and the burden of hospitalization over one year in stable outpatients remains limited. This study aimed to assess the relationship between PNI and liver-related hospitalization outcomes, as well as 12-month mortality, in outpatients with cirrhosis. In this retrospective single-center study, 125 adult patients with stable cirrhosis were analyzed, focusing on baseline demographic, clinical, laboratory, and 12-month follow-up data. Logistic regression was employed to evaluate the incidence of hospitalization in the entire cohort, while Poisson regression assessed hospital admission counts among patients with complete follow-up, and linear regression investigated the total length of stay for hospitalized patients.The results indicated that lower PNI values were significantly correlated with an increased likelihood of hospitalization and a higher frequency of hospital admissions within one year. In multivariable analysis, PNI remained an independent predictor of hospitalization (odds ratio [OR] = 0.655, 95% confidence interval [CI]: 0.530-0.809, p < 0.001) and admission frequency (incidence rate ratio [IRR] = 0.90, 95% CI: 0.848-0.955, p < 0.001), while no significant association was found with the length of hospital stay. Each 5-unit increase in PNI corresponded to substantially lower odds of hospitalization (OR = 0.121, 95% CI: 0.042-0.347) and a reduced admission rate (IRR = 0.59, 95% CI: 0.444-0.794). Additionally, PNI exhibited significant negative correlations with the Model for End-Stage Liver Disease-Sodium (MELD-Na) and Child-Turcotte-Pugh scores (all p < 0.001). PNI also demonstrated strong predictive validity for 12-month cirrhosis-related mortality, with an area under the curve (AUC) of 0.873 (95% CI: 0.802-0.943) and an optimal cut-off value of 38.5. Patients with a PNI ≤38.5 experienced significantly reduced 12-month survival (log-rank p < 0.001). These findings suggest that PNI may serve as a straightforward and accessible marker for nutritional assessment and early risk stratification in stable outpatients with cirrhosis.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long non-coding RNAs in neurodegenerative diseases - Molecular mechanisms, liquid biopsy biomarkers, and therapeutic targets: A review.","authors":"Yipeng Cheng, Mingyue Qiu, Zhijie Yu, Xu Tang, Jingjing Zhang","doi":"10.17305/bb.2026.13978","DOIUrl":"https://doi.org/10.17305/bb.2026.13978","url":null,"abstract":"<p><p>Neurodegenerative diseases (NDDs), such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington's disease (HD), are age-related disorders characterized by progressive neuronal loss, cognitive decline, and limited options for disease-modifying treatments. Increasing evidence suggests that long non-coding RNAs (lncRNAs) play significant roles in neurodevelopment, neuronal homeostasis, and disease progression; however, their involvement in shared pathogenic pathways and clinical applications remains inadequately defined. This review consolidates recent experimental, transcriptomic, bioinformatic, and emerging clinical findings regarding the role of lncRNAs in NDDs. We examine how lncRNAs modulate common disease mechanisms, including protein misfolding and aggregation, neuroinflammation, mitochondrial dysfunction, ferroptosis, synaptic failure, and aging-related neurodegenerative processes. These regulatory functions occur through various mechanisms, including epigenetic modifications, transcriptional regulation, post-transcriptional processes, and RNA-protein interactions, as well as novel mechanisms such as liquid-liquid phase separation (LLPS), peptide coding, and exosome-mediated intercellular communication. Current evidence supports the potential of lncRNAs as minimally invasive liquid biopsy biomarkers, detectable in blood, cerebrospinal fluid (CSF), and extracellular vesicles. Additionally, lncRNAs may serve as therapeutic targets through antisense oligonucleotides (ASOs), gene editing, and engineered delivery platforms. Overall, lncRNAs have emerged as central molecular regulators and promising candidates for translation in NDDs. Nonetheless, challenges related to specificity, validation, delivery across the blood-brain barrier, and clinical standardization must be addressed before their routine application in precision neurology.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative ultrasound in breast-conserving surgery for palpable breast cancer: Association with surgical margins and resection volume.","authors":"İsmail Zihni, Osman Cem Yilmaz, Meliha Ülkü Güzel","doi":"10.17305/bb.2026.13831","DOIUrl":"https://doi.org/10.17305/bb.2026.13831","url":null,"abstract":"<p><p>Accurate determination of surgical margins is a crucial aspect of breast-conserving surgery (BCS) for early-stage breast cancer. Traditionally, tumor localization has been performed through palpation followed by frozen section analysis. However, intraoperative ultrasonography (US) presents a practical and cost-effective alternative, particularly for small or poorly palpable lesions. This study aimed to evaluate the relationship between the use of intraoperative US and surgical outcomes in BCS. We conducted a retrospective analysis of 180 female patients with early-stage breast cancer who underwent surgery from 2020 to 2023, excluding those who received neoadjuvant therapy. Patients were classified into two groups based on the application of intraoperative US. We compared demographic, histopathological, and surgical parameters, including tumor diameter, resection volume, tumor diameter-to-resection volume ratio, closest surgical margin, and the necessity for cavity re-excision. Intraoperative US was utilized in 51.1% of cases (n=92). Tumor size was notably smaller in the intraoperative US group compared to the palpation-guided group (median 15 mm vs. 20 mm, p=0.019). Additionally, the median resection volume was significantly lower in the US group (64.65 cm³ vs. 130.91 cm³, p=0.001). The tumor diameter-to-resection volume ratio was higher in the intraoperative US group (0.03 vs. 0.02, p=0.044), indicating more precise tumor-targeted excision. Cavity re-excision rates were comparable between the two groups (10.9% vs. 15.9%, p=0.482), and no postoperative positive margins were recorded. Intraoperative ultrasonography was associated with adequate surgical margins and reduced resection volumes in breast-conserving surgery, suggesting its potential to enhance tissue preservation without increasing re-excision rates.</p>","PeriodicalId":72398,"journal":{"name":"Biomolecules & biomedicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147596171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}