Advanced Biomedical Research最新文献_第5页

A Review on the Cytotoxicity and Antibacterial Effect of Marine Organisms of Persian Gulf. 波斯湾海洋生物的细胞毒性及抗菌作用研究进展。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_290_22

Seyed Erfan Mousavi, Sheyda Razaghi, Nafiseh Emami, Afsaneh Yegdaneh

引用次数: 0

Two Distinct Deleterious Causative Variants in a Family with Multiple Cancer-Affected Patients. 多癌患者家族中两种不同的有害致病变异

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_366_22

Erfan Khorram, Mohammad A Tabatabaiefar, Mehrdad Zeinalian

{"title":"Two Distinct Deleterious Causative Variants in a Family with Multiple Cancer-Affected Patients.","authors":"Erfan Khorram, Mohammad A Tabatabaiefar, Mehrdad Zeinalian","doi":"10.4103/abr.abr_366_22","DOIUrl":"https://doi.org/10.4103/abr.abr_366_22","url":null,"abstract":"Background: Only 5 to 10% of cancers are hereditary, but they are particularly important since they can be passed down from generation to generation, and family members are at elevated risk. Although screening methods are one of the essential strategies for dealing with hereditary cancers, they do not have high specificity and sensitivity. The emergence of whole-exome sequencing (WES) causes a significant increase in the diagnostic rate of cancer-causing variants in at-risk families.Materials and methods: We performed WES on the proband's DNA sample from an Iranian family with multiple cancer-affected members to identify potential causative variants. Multiple in silico tools were used to evaluate the candidate variants' pathogenicity and their effects on the protein's structure, function, and stability. Moreover, the candidate variants were co-segregated in the family with Sanger sequencing.Results: The WES data analysis identified two pathogenic variants (CHEK2: NM_007194.4: c.538C>T, p.Arg180Cys and MLH1: NM_000249.4, c.844G>A, p.Ala282Thr). Sanger sequencing data showed each of the variants was incompletely segregated with phenotype, but both of them explained the patient's phenotype together. Also, the structural analysis demonstrated that due to the variant (c.538C>T), a salt bridge between arginine 180 and glutamic acid 149 was lost. Indeed, several protein stability tools described both variants as destabilizing.Conclusion: Herein, we interestingly identify two distinct deleterious causative variants (CHEK2: NM_007194.4: c.538C>T, p.Arg180Cys and MLH1: NM_000249.4, c.844G>A, p.Ala282Thr) in a family with several cancer-affected members. Furthermore, this study's findings established the utility of WES in the genetic diagnostics of cancer.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"203"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/89/ABR-12-203.PMC10492615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10213726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Glycopeptide-Resistant Genotypes among Clinical Isolates of Enterococcus with Van B Phenotype. Van B型肠球菌临床分离株糖肽耐药基因型的研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_133_21

Reena Rajan

{"title":"A Study on Glycopeptide-Resistant Genotypes among Clinical Isolates of Enterococcus with Van B Phenotype.","authors":"Reena Rajan","doi":"10.4103/abr.abr_133_21","DOIUrl":"https://doi.org/10.4103/abr.abr_133_21","url":null,"abstract":"Background: Enterococci, although remarked as harmless commensals of the intestinal tract of humans and animals, have become a significant source of infection in hospitalized patients worldwide.The present study aimed to isolate and identify enterococci from clinical samples and to determine the genotypic characteristics of vancomycin-resistant enterococcus isolates.Materials and methods: A total of 774 isolates of enterococci from clinical samples were identified to the species level, and their anti-microbial susceptibility pattern was determined by Kirby Bauer Disk Diffusion method and Vitek 2 automated system. Screening for vancomycin resistance was performed by using brain heart infusion agar containing 6 micrograms/ml of vancomycin. The minimum inhibitory concentration (MIC) of vancomycin was determined by the agar dilution method and Epsilometer test (E test). Genotyping was carried out for resistant isolates by multiplex polymerase chain reaction.Results: Thirty (3.88%) isolates were resistant to vancomycin by agar screen method. Five isolates showed intermediate resistance with anMIC of 8-16 micrograms/ml for vancomycin by agar dilution. By the E test, two isolates displayed an MIC of ≥32 micrograms/ml for vancomycin and one isolate showed an MICof ≥32 micrograms/ml for teicoplanin.Van A was the common genotype isolated. The present study reports isolates of Enterococcus faecalis with a susceptible MIC for glycopeptide and the presence of the Van A gene.Conclusion: Heterogeneous resistance among clinical isolates of Enterococcus faecalis was observed in our study. The predominant phenotype and genotype detected among clinical isolates were Van A.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"196"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/09/ABR-12-196.PMC10492609.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study of Dialectical Behavior Therapy and Aripiprazole on Marital Instability of in Patients with Hypersexual. 辩证行为疗法与阿立哌唑治疗性亢进症患者婚姻不稳定的比较研究。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_161_22

Zahra Tavakoli, Hasan Rezaei-Jamalouei, Hamid Kazemi-Zahrani, Mohammad Hatef Khorrami, Iman Ghanaat

{"title":"A Comparative Study of Dialectical Behavior Therapy and Aripiprazole on Marital Instability of in Patients with Hypersexual.","authors":"Zahra Tavakoli, Hasan Rezaei-Jamalouei, Hamid Kazemi-Zahrani, Mohammad Hatef Khorrami, Iman Ghanaat","doi":"10.4103/abr.abr_161_22","DOIUrl":"https://doi.org/10.4103/abr.abr_161_22","url":null,"abstract":"Background: Sexual desire and sexual activity are natural needs of human beings, which can be problematic and lead to various sexual disorders, if not used in the right way, including hypersexuality. The present study aimed to compare the effect of dialectical behavior therapy (DBT) and aripiprazole drug on marital instability in patients with hypersexuality.Materials and methods: This experimental case--control Pretest--Posttest Control Group Design with follow up was done on 27 male and female patients with hypersexuality having at least a higher education degree selected from four hospitals and psychiatric centers including Khorshid Hospital, Asgariyeh Specialized Hospital, Farhangian Clinic and Imam Reza Medical Center in Isfahan and were randomly assigned to two groups of treatment (nine patients in every group) and one group of control (nine patients) after adjusting the age and gender. Pretest phase was done for both three groups using Marital Instability Index (MII). The first treatment group underwent DBT intervention for eight sessions of 2 hours (once a week), and the second experimental group was prescribed aripiprazole for 2 months. Afterwards, the posttest and follow-up were performed for all the three groups. The data were analyzed using SPSS 24 and multivariate analysis of covariance (MANCOVA).Results: The findings showed that DBT and aripiprazole had little effect on the problem of marital instability in patients with hypersexuality (p > 0.05); also, there was no significant difference between the effect of DBT and aripiprazole (p > 0.05).Conclusion: DBT and the drug aripiprazole cannot have a significant effect on the marital instability in patients.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"190"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/a2/ABR-12-190.PMC10492616.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation and Comparison of Sensitivity and Specificity of Ultrasonography in Placenta Accreta Diagnosis in the Second and Third Trimesters. 超声诊断妊娠中晚期增生性胎盘的敏感性和特异性评价及比较。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_188_22

Minoo Movahedi, Farinaz Farahbod, Mahsa Mootamedi

{"title":"Evaluation and Comparison of Sensitivity and Specificity of Ultrasonography in Placenta Accreta Diagnosis in the Second and Third Trimesters.","authors":"Minoo Movahedi, Farinaz Farahbod, Mahsa Mootamedi","doi":"10.4103/abr.abr_188_22","DOIUrl":"https://doi.org/10.4103/abr.abr_188_22","url":null,"abstract":"Background: Ultrasound is the selected technique for the detection of placenta accreta spectrum (PAS). This method can detect PAS in 80%-50% of cases. This study aimed to assess and compare the sensitivity and specificity of ultrasonography in the diagnosis of PAS after the first trimester.Materials and methods: In this prospective study that was performed in 2020-2021 on 79 patients at high risk of PAS, all cases underwent ultrasonography in both 18-22 weeks of gestational age (GA) and 32-34 weeks of GA for evaluation of accreta. As per the risk factors, the delivery plan for all mothers was cesarean section. During the cesarean section, the placenta was examined for accreta, and if it was attached to the uterus, a diagnosis of placenta accreta was ascertained and a total abdominal hysterectomy was performed if the patient's bleeding was not controlled during the operation. The final diagnosis of PAS was made based on the pathology report.Results: Ultrasound evaluation for PAS in 18-22 weeks of GA had 79.17% specificity, 51.61% sensitivity, 61.54% positive predictive value, and 71.70% negative predictive value. Ultrasound imaging for PAS in 32-34 weeks of GA had 60.8% specificity, 90% sensitivity, 62.52% positive predictive value, and 90.33% negative predictive value.Conclusion: It should be concluded that PAS is a critical condition and if the patient is diagnosed in the second or third trimester, special strategies should be applied.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"188"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/19/06/ABR-12-188.PMC10492602.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Apoptotic Impacts of Hazelnut Oil on the Colorectal Cancer Cell Line. 榛子油对结直肠癌细胞凋亡影响的研究。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_297_21

Mina Ramezan, Hamideh Mahmoodzadeh Hosseini, Ali Salimi, Yousef Ramezan

{"title":"Study of the Apoptotic Impacts of Hazelnut Oil on the Colorectal Cancer Cell Line.","authors":"Mina Ramezan, Hamideh Mahmoodzadeh Hosseini, Ali Salimi, Yousef Ramezan","doi":"10.4103/abr.abr_297_21","DOIUrl":"https://doi.org/10.4103/abr.abr_297_21","url":null,"abstract":"Background: Hazelnut oil has a unique structure with a high oleic acid content, tocopherol, tocotrienols, and other bioactive compounds, such as phytosterols. These biochemical compounds have been widely studied because of their potential health properties. Understanding the process of apoptosis is the basis of new therapies contributing to cancer cells' death. Recently, the potential role of the evolutionary-reserved bcl-2 protein family in tumor progression and prognosis of some malignancies has been addressed in several studies. The present study is aimed at evaluating the effect of apoptotic properties of hazelnut oil on colorectal cancer cells through the major members of this family (bax and bcl-2).Materials and methods: MTT assay, apoptotic cell staining (using Annexin V and propidium iodide), flow cytometry, and real-time PCR were used to evaluate the toxicity, percentage of apoptotic cells, and bax and bcl-2 genes' expression after exposing HT29 cells to hazelnut oil.Results: After hazelnut treatment, significant decreases in cell viability, and the gene expression of bax and bcl-2 were observed compared to the control group (P < 0.05). In addition, the total percentage of apoptotic cells after hazelnut oil treatment showed a significant increase in comparison with the negative control group (P < 0.05).Conclusion: Hazelnut oil appears to cause the death of cancerous cells through an apoptotic mechanism.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"76"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/67/38/ABR-12-76.PMC10186048.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9544488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells. Nisin作为强效抗生素对结直肠癌细胞的抗黏附作用。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_267_21

Hesam Soleimanifar, Hamideh Mahmoodzadeh Hosseini, Sadra Samavarchi Tehrani, Seyed Ali Mirhosseini

{"title":"The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells.","authors":"Hesam Soleimanifar, Hamideh Mahmoodzadeh Hosseini, Sadra Samavarchi Tehrani, Seyed Ali Mirhosseini","doi":"10.4103/abr.abr_267_21","DOIUrl":"https://doi.org/10.4103/abr.abr_267_21","url":null,"abstract":"Background: Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by Lactococcus lactis. The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, mmp-2 and mmp-9, in the colorectal cancer cell line.Materials and methods: For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.Results: Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability (P < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and mmp-2 and mmp-9 gene expressions (P < 0.05).Conclusion: Our findings suggested that Nisin could prevent metastasis and cancer progression.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"113"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/1e/ABR-12-113.PMC10241620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Interfering Substance in Gel Tubes Affects Vitamin D Measurement by HPLC. 凝胶管中干扰物质对HPLC测定维生素D的影响。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_252_22

Mohammad Reza Haeri, Narges Emamnejad

{"title":"An Interfering Substance in Gel Tubes Affects Vitamin D Measurement by HPLC.","authors":"Mohammad Reza Haeri, Narges Emamnejad","doi":"10.4103/abr.abr_252_22","DOIUrl":"https://doi.org/10.4103/abr.abr_252_22","url":null,"abstract":"The demand for measuring vitamin D has increased dramatically, thus vitamin D measurement is one of the most frequently requested laboratory tests.[1] One of the most common methods to measure 25‐hydroxy vitamin D3 (25‐OH‐D3) is high‐performance liquid chromatography (HPLC) with enough reliability and high selectivity.[2] There is little information about the potential impact of blood collection tubes on 25(OH) D3 concentrations. However, many factors may affect the accuracy of the measurements, mainly pre‐analytical variables such as sample type and interfering factors.[3] The type of blood samples (plasma or serum) or collecting tube (plain or clot‐activating tube) sent from the hospital wards to the laboratory may vary, depending on the tests requested for the patient. The question of this study was whether the amounts of 25(OH) D3 measured by the HPLC method in serum (prepared in tubes containing gel and clot activator) and plasma are the same. For this purpose, blood samples from eight patients were collected simultaneously in tubes containing gel and clot activator, and in tubes containing EDTA (without gel). All tubes were centrifuged for 10 min at 3000 × g. Further, 25(OH) D3 was measured in all samples by HPLC (Agilent, USA) equipped with a C18 column and ultraviolet (UV) detector adjusted to 264 nm.[4] The mobile phase consisted of acetonitrile/methanol (90/10). To prepare samples, 400 μL of the patient sample and 400 μL of the precipitation and extraction reagents were dispensed into test tubes. To obtain a precipitate, the tubes were vortex‐mixed for 10 s and centrifuged at 10,000 RCF for 5 min. Finally, 250 microliters from the supernatant were injected into the HPLC, the mobile phase was applied with a flow rate of 1 mL/min in isocratic elution mode. Results were compared with the student’s t‐test using the GraphPad Prism 8.2.1 software. The significance level was defined as P ≤ 0.05.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"104"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/08/d9/ABR-12-104.PMC10241623.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9599252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is Chronic Kidney Disease, a Predictor of In-Hospital Mortality in Coronavirus Disease 2019 (COVID-19) Patients? 慢性肾脏疾病是2019冠状病毒病(COVID-19)患者住院死亡率的预测因子吗?

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_352_21

Firouzeh Moeinzadeh, Vahideh Raeisi, Media Babahajiani, Mojgan Mortazavi, Samaneh Pourajam, Shiva Seirafian, Mohammad Shirzadi, Shahram Taheri, Mehrdad Salahi, Marjan Mansourian, Arash Toghyani, Zahra Zamani

{"title":"Is Chronic Kidney Disease, a Predictor of In-Hospital Mortality in Coronavirus Disease 2019 (COVID-19) Patients?","authors":"Firouzeh Moeinzadeh, Vahideh Raeisi, Media Babahajiani, Mojgan Mortazavi, Samaneh Pourajam, Shiva Seirafian, Mohammad Shirzadi, Shahram Taheri, Mehrdad Salahi, Marjan Mansourian, Arash Toghyani, Zahra Zamani","doi":"10.4103/abr.abr_352_21","DOIUrl":"https://doi.org/10.4103/abr.abr_352_21","url":null,"abstract":"Background: Chronic kidney disease (CKD) is an important comorbidity in Coronavirus Disease 2019 (COVID-19) patients considering its high prevalence. We aimed to figure out the relationship between CKD and COVID-19 mortality in this study.Materials and methods: In total, 116 CKD patients (estimated glomerular filtration rate [eGFR] lower than 60 mL/min/1.73 m2) and 147 control subjects confirmed with COVID-19 were studied. Data regarding demographics, sign and symptoms, laboratory findings, and chest computed tomography were collected. Association between CKD and in-hospital mortality were analyzed using logistic regression models adjusted for confounders.Results: Mortality rate was significantly higher in CKD than non-CKD (30.17 vs 4.76, P < 0.001) COVID-19 patients. Multivariate logistic regression showed that CKD was significantly correlated with in-hospital mortality in the total sample (Odds ratio (OR) = 8.64, confidence interval (CI): 3.67-20.35) and gender subgroups (females: OR = 4.77, CI: 1.38-16.40, males: OR = 13.43, CI: 3.85-46.87) (P < 0.05) of COVID-19 patients in the crude model. Whereas, the correlation did not remain significant in the fully adjusted model in the total sample (OR = 1.70, CI: 0.35-8.19) and gender subgroups (females: OR = 1.07 CI: 0.06-19.82, males: OR = 0.87, CI: 0.07-10.33) (P > 0.05) of COVID-19 patients.Conclusion: This study suggested an independent association between CKD and in-hospital mortality in COVID-19 patients. Therefore, more intensive surveillance of COVID-19 patients with CKD is to be warranted.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"39"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/52/58/ABR-12-39.PMC10086659.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9674916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Cancer/Testis Antigens Related to Gastric Cancer Prognosis Based on Co-Expression Network and Integrated Transcriptome Analyses. 基于共表达网络和整合转录组分析的胃癌预后相关癌/睾丸抗原鉴定

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_400_21

Sara Ansari, Parvaneh Nikpour

{"title":"Identification of Cancer/Testis Antigens Related to Gastric Cancer Prognosis Based on Co-Expression Network and Integrated Transcriptome Analyses.","authors":"Sara Ansari, Parvaneh Nikpour","doi":"10.4103/abr.abr_400_21","DOIUrl":"https://doi.org/10.4103/abr.abr_400_21","url":null,"abstract":"Background: Gastric cancer is a worldwide life-threatening cancer. The underlying cause of it is still unknown. We have noticed that some cancer/testis antigens (CTAs) are up-regulated in gastric cancer. The role of these genes in gastric cancer development is not fully understood. The main aim of the current study was to comprehensively investigate CTAs' expression and function in stomach adenocarcinoma (STAD).Materials and methods: A comprehensive list of CTA genes was compiled from different databases. Transcriptome profiles of STAD were downloaded from the cancer genome atlas (TCGA) database and analyzed. Differentially-expressed CTAs were identified. Pathway enrichment analysis, weighted gene correlation network analysis (WGCNA), and overall survival (OS) analysis were performed on differentially-expressed CTA genes.Results: Pathway enrichment analysis indicates that CTA genes are involved in protein binding, ribonucleic acid processing, and reproductive tissues. WGCNA showed that six differentially-expressed CTA genes, namely Melanoma antigen gene (MAGE) family member A3, A6, A12 and chondrosarcoma associated gene (CSAG) 1, 2, and 3, were correlated. Up-regulation of MAGEA11, MAGEC3, Per ARNT SIM domain containing 1 (PASD1), placenta-specific protein 1 (PLAC1) and sperm protein associated with the nucleus X-linked family member (SPANXB1) were significantly associated with lower OS of patients.Conclusion: MAGEA11, MAGEC3, PASD1, PLAC1, and SPANXB1 can be investigated as prognostic biomarkers in basic and clinical studies. Further functional experiments are needed to understand the exact interaction mechanisms of these genes.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"52"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/8e/ABR-12-52.PMC10086657.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9360022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1