Advanced Biomedical Research最新文献_第5页

A Comparative Study on the Usefulness of Fractional CO₂ and Fractional Er:YAG in Acne Scars: A Split-Face Trial. 分数CO2和分数Er:YAG治疗痤疮疤痕的比较研究:一项裂面试验。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_330_21

Fatemeh Mokhtari, Zahra Safavi, Gita Faghihi, Ali Asilian, Zabihollah Shahmoradi

{"title":"A Comparative Study on the Usefulness of Fractional CO2 and Fractional Er:YAG in Acne Scars: A Split-Face Trial.","authors":"Fatemeh Mokhtari, Zahra Safavi, Gita Faghihi, Ali Asilian, Zabihollah Shahmoradi","doi":"10.4103/abr.abr_330_21","DOIUrl":"https://doi.org/10.4103/abr.abr_330_21","url":null,"abstract":"Background: Acne is a dermatologic condition with a high burden in terms of psychosocial consequences as a result of scars remaining on the skin. Its effects are severe in adolescence and finding treatments with short therapy courses, superior results, and fewer adverse effects are of high importance. Materials and Methods: We included 30 individuals with acne vulgaris scars in Al-Zahra academic training hospital from June 2018 to Jan 2019. Each individual received both fractional CO2 and fractional Er:YAG lasers on right and left sides of the face, respectively. Three sessions of laser treatment were applied to each side with one-month intervals. Results were evaluated by patients according to subjective satisfaction and physicians’ assessment and photo evaluation by two blinded dermatologists. Improvement was graded by a quartile grading scale: less than 25%: mild, 25% to 50%: moderate, 51% to 75%: good, and 76% to 100%: excellent response. Assessments were obtained at baseline and one month after the last visit. Results: Based on subjective satisfaction (p < 0.05) and physicians’ assessment (p < 0.01), fractional CO2 laser was significantly more effective than Erbium:YAG laser. Also, Post-treatment side effects were mild and transient in both groups. Conclusion: Laser therapies are common in the treatment of scars and each modality has special advantages and disadvantages. Choosing among them should be based on various criteria. Fractional CO2 lasers have been revealed favorable results in most reports. Large comprehensive trials could help experts in choosing among alternatives for different subgroups.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"90"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/34/e7/ABR-12-90.PMC10241645.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9599251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spinal cord tumors: A review of demographic characteristics and treatment follow-up of patients in Isfahan city, Iran 脊髓肿瘤:伊朗伊斯法罕市患者的人口统计学特征和治疗随访的回顾

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_168_23

Mehdi Shafiei, Mehdi Mahmoodkhani, Majid Rezvani, Majid Aliyari, DonyaS Tehrani

{"title":"Spinal cord tumors: A review of demographic characteristics and treatment follow-up of patients in Isfahan city, Iran","authors":"Mehdi Shafiei, Mehdi Mahmoodkhani, Majid Rezvani, Majid Aliyari, DonyaS Tehrani","doi":"10.4103/abr.abr_168_23","DOIUrl":"https://doi.org/10.4103/abr.abr_168_23","url":null,"abstract":"Background: Given that spinal cord tumors cause damage to the central nervous system, the involvement of body organs, patients’ reduced quality of life, and the follow-up of patients should be performed with more sensitivity. Therefore, the present study aimed to determine the demographic characteristics and treatment follow-up of patients with spinal cord tumors. Materials and Methods: The present descriptive-analytical and cross-sectional study investigated all patients with spinal cord tumors in 2022 who visited the Kashani Hospital Isfahan. A total of 31 patients were included in the study using the census sampling method. The data were collected by a checklist and three pain scales, namely the Dennis pain scale, the Frankel Grade classification, and Nurick’s grading scales. Results: The present cross-sectional study examined 31 patients with spinal cord tumors who visited the Kashani Hospital in Isfahan. Most spinal cord tumors were ependymoma with nine cases (29%) and neurofibroma with six cases (19.35%). The tumor site was thoracic in 10 cases (33.33%). In the follow-up phase, 31 patients (53.4%) returned to their normal life with full recovery and 10 (17.2%) died. Three (75%) out of four cases of tumor recurrence had ependymoma tumors and one (25%) had astrocytoma. There was a significant difference between the patients’ pre- and post-operative pain severity and functional status as the post-operative scores decreased on all three scales. Conclusion: The results may contribute to improve the pre-operative planning, optimization, and monitoring of the patient’s recovery after the operation.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135318132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Posterior leaflet of mitral valve—Is it really tri-scalloped? —A morphological and morphometric study in North Indian cadaveric hearts 二尖瓣后小叶-它真的是三扇形的吗?北印度尸体心脏的形态学和形态计量学研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_330_22

Ashwini Kumar, Bhagya Shree, RajanKumar Singla, Sachin Soni, SanjayKumar Sharma, SushantSwaroop Das, Nidhi Puri

{"title":"Posterior leaflet of mitral valve—Is it really tri-scalloped? —A morphological and morphometric study in North Indian cadaveric hearts","authors":"Ashwini Kumar, Bhagya Shree, RajanKumar Singla, Sachin Soni, SanjayKumar Sharma, SushantSwaroop Das, Nidhi Puri","doi":"10.4103/abr.abr_330_22","DOIUrl":"https://doi.org/10.4103/abr.abr_330_22","url":null,"abstract":"Background: Maintaining normal left ventricular geometry and function depends on the mitral valve's normal integrity. Irreparable damage to the mitral valve calls for its replacement using either a valve made up of biological tissue or metal, pyrolytic carbon, and similar materials. Materials and Methods: The material consists of 50 formalin-fixed adults, seemingly normal cadaveric hearts of either sex which were received from the Department of Anatomy of various institutes in the north region. These hearts were cut open to access the mitral valve in the left ventricle. Results: In this study, the posterior leaflet was semi-oval in shape being 3.72 cm wide at the base. Usually said to be tri-scalloped, interestingly, it was found so only in 56% of the hearts; being bi-scalloped in 20% and single-cusped in 16% of the hearts. Even four scallops and six scallops were observed in three (6%) and one (2%) hearts, respectively. Conclusions: To conclude, notable variation has been seen in the scallops of posterolateral cusps in the present study. The number of scallops varies greatly as single, double, three, four, or tetra-scalloped and most significant six or hexa-scalloped which has never been reported in the previous studies. To understand the rationale behind each unique architectural layout, such noticeable variations are crucial for scientists around the world. Cardiothoracic surgeons could find this information valuable for mitral valve surgery repair.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"165 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135212535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Vitrectomy: An Effective Treatment for Acute Postcataract Surgery Endophthalmitis. 早期玻璃体切除术:急性白内障术后眼内炎的有效治疗方法。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_44_21

Farhad Fazel Najafabadi, Ali Salehi, Mohammad Hadi Vaezi, Heshmatollah Ghanbari, Mohamadreza Fazel Najafabadi, Nima Koosha, Zahra Jafarzadeh

{"title":"Early Vitrectomy: An Effective Treatment for Acute Postcataract Surgery Endophthalmitis.","authors":"Farhad Fazel Najafabadi, Ali Salehi, Mohammad Hadi Vaezi, Heshmatollah Ghanbari, Mohamadreza Fazel Najafabadi, Nima Koosha, Zahra Jafarzadeh","doi":"10.4103/abr.abr_44_21","DOIUrl":"https://doi.org/10.4103/abr.abr_44_21","url":null,"abstract":"Background: Endophthalmitis is a rare but a high morbid complication after cataract surgery, and a gold standard treatment is not recommended yet. In this study, we aim to evaluate the effect of early vitrectomy on the visual acuity of patients with postcataract endophthalmitis.Materials and methods: This study was a single-arm clinical trial on 27 patients with postcataract surgery endophthalmitis. Early vitrectomy was the intervention. Visual acuity as the primary outcome was evaluated and compared at baseline, at discharge, and 1 and 3 months after the intervention.Results: From 27 patients who included in our study, six patients gain favorable visual acuity of 5/10 and above (success rate = 22%), and four of them have no improvement in their visual acuity. Retinal detachment was reported as a complication in just one case. Negative culture was a predictor for success in terms of visual acuity after the surgery. All patients who gain favorable results, presented in the first 15 days after the cataract surgery.Conclusion: The result of our study showed that, considering complete, early vitrectomy for the treatment of postcataract surgery endophthalmitis, especially for those who presented in the first 15 days of cataract surgery and for those who have negative culture is promising.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"79"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/21/c2/ABR-12-79.PMC10186043.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9845423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormone Therapy with or without Platelet-Rich Plasma (PRP) for Treatment Asherman Syndrome; A Randomized Clinical Trial. 富血小板血浆(PRP)激素治疗阿什曼综合征一项随机临床试验。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_411_21

Elham Naghshineh, Safoura Rouholamin, Zahra Derakhshandeh

{"title":"Hormone Therapy with or without Platelet-Rich Plasma (PRP) for Treatment Asherman Syndrome; A Randomized Clinical Trial.","authors":"Elham Naghshineh, Safoura Rouholamin, Zahra Derakhshandeh","doi":"10.4103/abr.abr_411_21","DOIUrl":"https://doi.org/10.4103/abr.abr_411_21","url":null,"abstract":"Background: Asherman syndrome is a controversial issue in obstetrics and gynecology without any consensus on its management and treatment. It is characterized by variable lesions inside the uterine cavity and also causes menstrual irregularities, infertility, and placental abnormalities. The study aimed to assess the platelet-rich plasma (PRP) effect in women with intrauterine adhesions by evaluating the improvement of the menstrual cycle and intrauterine adhesion (IUA) stage.Materials and methods: This clinical trial study was performed on 60 women with Asherman syndrome in two groups of 30. For the first group, only hormone therapy was performed and for the second group, hormone therapy with platelet-rich plasma after hysteroscopy. Recovery of Asherman syndrome and IUA stage was assessed at 6 to 8 weeks after hysteroscopy and compared between the two groups.Results: Our results demonstrated that there was no significant difference between demographic data in the two groups as well as the menstrual pattern of both groups before or after treatment (P > 0.05). Frequency distribution of IUA after the intervention in the PRP + hormone therapy group in grade I, II, and III were equal to 73.3%, 20%, and 6.7% and in the hormone therapy group were 53.3%, 26.7%, and 20%, respectively (P = 0.22). In addition, hypo menorrhea was observed in 33.3% of PRP + hormone therapy group and 40% of the hormone therapy group with no significant difference between the two groups (P = 0.71).Conclusion: Hormone therapy with PRP compared to hormone therapy alone after routine surgical treatment had not a significant effect on the IUA stage, duration, and severity of menstruation.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"17"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/75/ABR-12-17.PMC10012034.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9132687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Quality Assessment of Computed Tomography Images using a Channelized Hoteling Observer: Optimization of Protocols in Clinical Practice. 使用通道化旅馆观察者的计算机断层成像质量评估:临床实践中方案的优化。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_353_21

Mohammad Reza Choopani, Iraj Abedi, Fatemeh Dalvand

{"title":"Quality Assessment of Computed Tomography Images using a Channelized Hoteling Observer: Optimization of Protocols in Clinical Practice.","authors":"Mohammad Reza Choopani, Iraj Abedi, Fatemeh Dalvand","doi":"10.4103/abr.abr_353_21","DOIUrl":"https://doi.org/10.4103/abr.abr_353_21","url":null,"abstract":"Background: This study investigated the feasibility of channelized hoteling observer (CHO) model in computed tomography (CT) protocol optimization regarding the image quality and patient exposure. While the utility of using model observers such as to optimize the clinical protocol is evident, the pitfalls associated with the use of this method in practice require investigation.Materials and methods: This study was performed using variable tube current and adaptive statistical iterative reconstruction (ASIR) level (ASIR 10% to ASIR 100%). Various criteria including noise, high-contrast spatial resolution, CHOs model were used to compare image quality at different captured levels. For the implementation of CHO, we first tuned the model in a restricted dataset and then it to the evaluation of a large dataset of images obtained with different reconstruction ASIR and filtered back projection (FBP) levels.Results: The results were promising in terms of CHO use for the stated purposes. Comparisons of the noise of reconstructed images with 30% ASIR and higher levels of noise in rebuilding images using the FBP approach showed a significant difference (P < 0.05). The spatial resolution obtained using various ASIR levels and tube currents were 0.8 pairs of lines per millimeter, which did not differ significantly from the FBP method (P > 0.05).Conclusions: Based on the results, using 80% ASIR can reduce the radiation dose on lungs, abdomen, and pelvis CT scans while maintaining image quality. Furthermore using ASIR 60% only for the reconstruction of lungs, abdomen, and pelvis images at standard radiation dose leads to optimal image quality.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/dc/ABR-12-8.PMC10012030.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9138624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Effect of Carvedilol in Preventing Right Ventricular Dysfunction in Breast Cancer Patients Receiving Anthracycline. 卡维地洛预防蒽环类药物治疗乳腺癌患者右心室功能障碍的效果评价。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_134_21

Mersede Karvandi, Mojtaba Ghadyani, Nahid Mohebbi, Mehdi Tabarraee, Sina Salari

{"title":"Evaluation of the Effect of Carvedilol in Preventing Right Ventricular Dysfunction in Breast Cancer Patients Receiving Anthracycline.","authors":"Mersede Karvandi, Mojtaba Ghadyani, Nahid Mohebbi, Mehdi Tabarraee, Sina Salari","doi":"10.4103/abr.abr_134_21","DOIUrl":"https://doi.org/10.4103/abr.abr_134_21","url":null,"abstract":"Background: Today, it has been shown that it is possible for right ventricular (RV) wall motion abnormalities or RV functional disorders to occur during cancer treatment. Now, considering the effect of carvedilol on beta 1, 2, and alpha receptors and its antioxidant properties, it seems that it can prevent RV abnormalities. Therefore, the aim of this study was to investigate the possible protective effects of carvedilol in preventing RV dysfunction in patients with breast cancer treated with anthracyclines.Materials and methods: The present single-blind clinical trial study was performed on 23 patients with breast cancer that 12 of them received only the anthracycline antineoplastic doxorubicin (Adriamycin®) chemotherapy (control group) and 11 patients received carvedilol in addition to anthracycline. To evaluate the effect of carvedilol, patients underwent transthoracic echocardiography before intervention and 2 weeks after the end of treatment with anthracyclines.Results: The two parameters of RV ejection fraction and RV fractional area change in the carvedilol group with a mean of 66.41% ± 8.10% and 51.85% ± 6.89% were slightly higher than the control group with a mean of 64.58% ± 6.83% and 50.48 ± 5.79%, respectively, which was not statistically significant (P > 0.05). In contrast, RV S wave tissue Doppler imaging (S-TDI) in the control group with a mean of 0.13 ± 0.02 m/s was significantly lower than the carvedilol group with a mean of 0.14 ± 0.02 m/s (P = 0.022).Conclusion: According to the results of the present study, the effect of using carvedilol as a preservative on improving RV function was seen compared to the control group, although this difference was not statistically significant.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/45/ABR-12-5.PMC10012032.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9188063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review on the Cytotoxicity and Antibacterial Effect of Marine Organisms of Persian Gulf. 波斯湾海洋生物的细胞毒性及抗菌作用研究进展。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_290_22

Seyed Erfan Mousavi, Sheyda Razaghi, Nafiseh Emami, Afsaneh Yegdaneh

引用次数: 0

Two Distinct Deleterious Causative Variants in a Family with Multiple Cancer-Affected Patients. 多癌患者家族中两种不同的有害致病变异

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_366_22

Erfan Khorram, Mohammad A Tabatabaiefar, Mehrdad Zeinalian

{"title":"Two Distinct Deleterious Causative Variants in a Family with Multiple Cancer-Affected Patients.","authors":"Erfan Khorram, Mohammad A Tabatabaiefar, Mehrdad Zeinalian","doi":"10.4103/abr.abr_366_22","DOIUrl":"https://doi.org/10.4103/abr.abr_366_22","url":null,"abstract":"Background: Only 5 to 10% of cancers are hereditary, but they are particularly important since they can be passed down from generation to generation, and family members are at elevated risk. Although screening methods are one of the essential strategies for dealing with hereditary cancers, they do not have high specificity and sensitivity. The emergence of whole-exome sequencing (WES) causes a significant increase in the diagnostic rate of cancer-causing variants in at-risk families.Materials and methods: We performed WES on the proband's DNA sample from an Iranian family with multiple cancer-affected members to identify potential causative variants. Multiple in silico tools were used to evaluate the candidate variants' pathogenicity and their effects on the protein's structure, function, and stability. Moreover, the candidate variants were co-segregated in the family with Sanger sequencing.Results: The WES data analysis identified two pathogenic variants (CHEK2: NM_007194.4: c.538C>T, p.Arg180Cys and MLH1: NM_000249.4, c.844G>A, p.Ala282Thr). Sanger sequencing data showed each of the variants was incompletely segregated with phenotype, but both of them explained the patient's phenotype together. Also, the structural analysis demonstrated that due to the variant (c.538C>T), a salt bridge between arginine 180 and glutamic acid 149 was lost. Indeed, several protein stability tools described both variants as destabilizing.Conclusion: Herein, we interestingly identify two distinct deleterious causative variants (CHEK2: NM_007194.4: c.538C>T, p.Arg180Cys and MLH1: NM_000249.4, c.844G>A, p.Ala282Thr) in a family with several cancer-affected members. Furthermore, this study's findings established the utility of WES in the genetic diagnostics of cancer.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"203"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/89/ABR-12-203.PMC10492615.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10213726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Glycopeptide-Resistant Genotypes among Clinical Isolates of Enterococcus with Van B Phenotype. Van B型肠球菌临床分离株糖肽耐药基因型的研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_133_21

Reena Rajan

{"title":"A Study on Glycopeptide-Resistant Genotypes among Clinical Isolates of Enterococcus with Van B Phenotype.","authors":"Reena Rajan","doi":"10.4103/abr.abr_133_21","DOIUrl":"https://doi.org/10.4103/abr.abr_133_21","url":null,"abstract":"Background: Enterococci, although remarked as harmless commensals of the intestinal tract of humans and animals, have become a significant source of infection in hospitalized patients worldwide.The present study aimed to isolate and identify enterococci from clinical samples and to determine the genotypic characteristics of vancomycin-resistant enterococcus isolates.Materials and methods: A total of 774 isolates of enterococci from clinical samples were identified to the species level, and their anti-microbial susceptibility pattern was determined by Kirby Bauer Disk Diffusion method and Vitek 2 automated system. Screening for vancomycin resistance was performed by using brain heart infusion agar containing 6 micrograms/ml of vancomycin. The minimum inhibitory concentration (MIC) of vancomycin was determined by the agar dilution method and Epsilometer test (E test). Genotyping was carried out for resistant isolates by multiplex polymerase chain reaction.Results: Thirty (3.88%) isolates were resistant to vancomycin by agar screen method. Five isolates showed intermediate resistance with anMIC of 8-16 micrograms/ml for vancomycin by agar dilution. By the E test, two isolates displayed an MIC of ≥32 micrograms/ml for vancomycin and one isolate showed an MICof ≥32 micrograms/ml for teicoplanin.Van A was the common genotype isolated. The present study reports isolates of Enterococcus faecalis with a susceptible MIC for glycopeptide and the presence of the Van A gene.Conclusion: Heterogeneous resistance among clinical isolates of Enterococcus faecalis was observed in our study. The predominant phenotype and genotype detected among clinical isolates were Van A.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"196"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/09/ABR-12-196.PMC10492609.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0