The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells.

Advanced Biomedical Research Pub Date : 2023-01-01 DOI:10.4103/abr.abr_267_21

Hesam Soleimanifar, Hamideh Mahmoodzadeh Hosseini, Sadra Samavarchi Tehrani, Seyed Ali Mirhosseini

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Abstract

Background: Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by Lactococcus lactis. The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, mmp-2 and mmp-9, in the colorectal cancer cell line.

Materials and methods: For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.

Results: Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability (P < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and mmp-2 and mmp-9 gene expressions (P < 0.05).

Conclusion: Our findings suggested that Nisin could prevent metastasis and cancer progression.

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Nisin作为强效抗生素对结直肠癌细胞的抗黏附作用。

背景:细菌素是一种由益生菌产生的抗菌肽。它们已被研究作为可能的治疗药物，并已被用于抑制食物中的细菌生长。乳链球菌素是乳球菌产生的一种具有抑菌和抗癌特性的强效菌素。本研究旨在探讨Nisin对结直肠癌细胞系细胞黏附及其相关基因mmp-2和mmp-9的影响。材料和方法:为此，用不同浓度的Nisin处理HT-29细胞，使用MTT法、细胞粘附法和实时荧光定量PCR评估细胞毒性、细胞粘附和基因表达。结果:Nisin浓度为32 ~ 1024 μg/ml时，细胞活力显著降低(P < 0.05)。128和256 μg/ml的Nisin显著降低了细胞黏附，降低了mmp-2和mmp-9基因的表达(P < 0.05)。结论:Nisin具有预防肿瘤转移和进展的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Advanced Biomedical Research

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