{"title":"Nisin作为强效抗生素对结直肠癌细胞的抗黏附作用。","authors":"Hesam Soleimanifar, Hamideh Mahmoodzadeh Hosseini, Sadra Samavarchi Tehrani, Seyed Ali Mirhosseini","doi":"10.4103/abr.abr_267_21","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by <i>Lactococcus lactis</i>. The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, <i>mmp-2</i> and <i>mmp-9</i>, in the colorectal cancer cell line.</p><p><strong>Materials and methods: </strong>For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.</p><p><strong>Results: </strong>Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability (<i>P</i> < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and <i>mmp</i>-2 and <i>mmp</i>-9 gene expressions (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Our findings suggested that Nisin could prevent metastasis and cancer progression.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"113"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/1e/ABR-12-113.PMC10241620.pdf","citationCount":"0","resultStr":"{\"title\":\"The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells.\",\"authors\":\"Hesam Soleimanifar, Hamideh Mahmoodzadeh Hosseini, Sadra Samavarchi Tehrani, Seyed Ali Mirhosseini\",\"doi\":\"10.4103/abr.abr_267_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by <i>Lactococcus lactis</i>. The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, <i>mmp-2</i> and <i>mmp-9</i>, in the colorectal cancer cell line.</p><p><strong>Materials and methods: </strong>For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.</p><p><strong>Results: </strong>Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability (<i>P</i> < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and <i>mmp</i>-2 and <i>mmp</i>-9 gene expressions (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Our findings suggested that Nisin could prevent metastasis and cancer progression.</p>\",\"PeriodicalId\":7225,\"journal\":{\"name\":\"Advanced Biomedical Research\",\"volume\":\"12 \",\"pages\":\"113\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/1e/ABR-12-113.PMC10241620.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Biomedical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/abr.abr_267_21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Biomedical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/abr.abr_267_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells.
Background: Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by Lactococcus lactis. The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, mmp-2 and mmp-9, in the colorectal cancer cell line.
Materials and methods: For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.
Results: Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability (P < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and mmp-2 and mmp-9 gene expressions (P < 0.05).
Conclusion: Our findings suggested that Nisin could prevent metastasis and cancer progression.
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