Neurochemical Research最新文献_第9页

Retraction Note: Prenatal Exposure to COVID-19 mRNA Vaccine BNT162b2 Induces Autism-Like Behaviors in Male Neonatal Rats: Insights into WNT and BDNF Signaling Perturbations 产前暴露于COVID-19 mRNA疫苗BNT162b2诱导雄性新生大鼠的自闭症样行为：对WNT和BDNF信号扰动的见解。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-19 DOI: 10.1007/s11064-025-04492-x

Mumin Alper Erdogan, Orkun Gurbuz, Mehmet Fatih Bozkurt, Oytun Erbas

引用次数: 0

ANA-12 Targets and Inhibits BDNF/TrkB Signaling to Alleviate Pain Behaviors in Rheumatoid Arthritis Mice ANA-12靶向并抑制BDNF/TrkB信号减轻类风湿关节炎小鼠的疼痛行为

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-17 DOI: 10.1007/s11064-025-04487-8

Man Yuan, Long Zhang, Ye Zheng, Min Xie

{"title":"ANA-12 Targets and Inhibits BDNF/TrkB Signaling to Alleviate Pain Behaviors in Rheumatoid Arthritis Mice","authors":"Man Yuan, Long Zhang, Ye Zheng, Min Xie","doi":"10.1007/s11064-025-04487-8","DOIUrl":"10.1007/s11064-025-04487-8","url":null,"abstract":"<div><p>Rheumatoid arthritis (RA) is a chronic, systemic, inflammatory disease. Sensitization of central pain pathways by pro-inflammatory mediators has been implicated in RA pain. Locus coeruleus (LC) functions in pain pathways. Brain-derived neurotrophic factor (BDNF) participates in the modulation of nociception and pain. A mouse model of RA immunized with collagen-induced arthritis (CIA) was used for investigating the mechanisms of pain relief by administration of the tropomyosin receptor kinase B (TrkB) receptor antagonist ANA-12. We measured the pain behaviors and locomotor activity and found increased pain sensitivity and locomotor deficit in RA mice; ANA-12 treatment reduced pain behaviors and promoted locomotor function recovery. The glial activation and increased activities of BDNF/TrkB and MAPK signal pathways were found in LC of RA mice. The components of NLRP3 inflammasome were all increased and consequently enhanced the production of pro-inflammatory cytokine interleukin (IL)-1β. Upon ANA-12 treatment, glial cell activation was reduced, BDNF/TrkB and MAPK pathways were suppressed, and the expression levels of the above-mentioned proteins were reduced. Finally, U251 cells were conducted to further confirm the regulatory mechanisms of ANA-12 on inflammation. The results showed the colocalization of BDNF/NLRP3/IL-1β and GFAP. ANA-12 treatment decreased the protein levels of BDNF, TrkB, MAPK, NLRP3, and caspase-1 in IL-1β-induced cells. Besides, ANA-12 treatment decreased NLRC4 and AIM2 inflammasomes both in RA mice and IL-1β-induced cells. These results suggested that ANA-12 alleviates hyperalgesia in RA mice by inhibiting BDNF/TrkB signaling in LC, thereby reducing glial cell activation and inflammatory cytokine release.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impairment of Spatial Working Memory but Preservation of Recognition Memory in Female Rats with Spontaneous Absence Seizures 自发性失神发作对雌性大鼠空间工作记忆损害及识别记忆保留的影响。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-17 DOI: 10.1007/s11064-025-04485-w

Mariana Neuparth-Sottomayor, Tatiana P. Morais, Mark Good, Ana Maria Sebastião, Giuseppe Di Giovanni, Vincenzo Crunelli, Sandra H. Vaz

{"title":"Impairment of Spatial Working Memory but Preservation of Recognition Memory in Female Rats with Spontaneous Absence Seizures","authors":"Mariana Neuparth-Sottomayor, Tatiana P. Morais, Mark Good, Ana Maria Sebastião, Giuseppe Di Giovanni, Vincenzo Crunelli, Sandra H. Vaz","doi":"10.1007/s11064-025-04485-w","DOIUrl":"10.1007/s11064-025-04485-w","url":null,"abstract":"<div><p>Epidemiological studies reveal gender-specific differences in epilepsy. Childhood absence epilepsy (CAE), which is more prevalent in females, is characterized by typical absence seizures (ASs) consisting of brief periods of unconsciousness, associated with 2.5–4 Hz spike-wave discharges (SWDs) in the electroencephalogram (EEG). Children with CAE often present neuropsychological comorbidities, including deficits in attention and executive function. In this study, we investigated anxiety-like behaviour and memory in female Genetic Absence Epilepsy Rat from Strasbourg (GAERS), a validated model of ASs, compared to Non-Epileptic Control (NEC) and Wistar rats. We found that female GAERS generally showed normal anxiety-like behaviour relative to both control strains, although some tests suggested a reduction in anxiety. Importantly, female GAERS showed impaired spatial working memory, while recognition memory was preserved. These findings when compared with previous data in males indicate that while anxiety levels in female GAERS are preserved as those of male GAERS, memory performance differs, with males showing impairments in both spatial working memory and recognition memory. These findings emphasize the importance of considering gender differences in both clinical and preclinical epilepsy research to better understand the neuropsychological comorbidities associates with ASs. This knowledge is crucial for the identification of gender-specific mechanism, as well as the development of gender-sensitive, personalized therapies targeting both seizures and associated cognitive impairments.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel GLP-1/GIP Dual Receptor Agonist Alleviates Neonatal Hypoxic-Ischemic Encephalopathy by Inhibiting TLR2/NF-κB/NLRP3 Mediated-Neuroinflammation 新型GLP-1/GIP双受体激动剂通过抑制TLR2/NF-κB/NLRP3介导的神经炎症减轻新生儿缺氧缺血性脑病：DA5-CH在新生儿缺氧缺血性脑病中的作用

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-17 DOI: 10.1007/s11064-025-04475-y

Weiqing Huang, Xionghui Wu, Shuting Chang, Xiaoming Peng, Xiao Li

{"title":"Novel GLP-1/GIP Dual Receptor Agonist Alleviates Neonatal Hypoxic-Ischemic Encephalopathy by Inhibiting TLR2/NF-κB/NLRP3 Mediated-Neuroinflammation","authors":"Weiqing Huang, Xionghui Wu, Shuting Chang, Xiaoming Peng, Xiao Li","doi":"10.1007/s11064-025-04475-y","DOIUrl":"10.1007/s11064-025-04475-y","url":null,"abstract":"<div><p>Hypoxic-ischemic encephalopathy (HIE) is an irreversible brain injury attributable to impaired blood oxygen delivery in the brain after perinatal asphyxia. The pathogeny of HIE is very complex, and there is still shortage of effective treatment. DA5-CH is a novel dual receptor agonist of glucose dependent insulin stimulating polypeptide (GIP) and glucagon like peptide-1 (GLP-1). However, the function and mechanism of DA5-CH in HIE remain unclear. In this paper, cultured cortical neurons were exposed to oxygen-glucose deprivation (OGD) and neonatal rats were subjected to hypoxic-ischemic damage to explore the protective effects of DA5-CH. Our work revealed that DA5-CH markedly increased cell viability, reduced intracellular ROS levels and DNA damage, and decreased cell apoptosis in OGD-treated cultured cortical neurons. In vivo, DA5-CH treatment significantly improved cognitive dysfunction and neuronal damage, decreased the infarct volume and neuronal death of hypoxic-ischemic (HI) neonatal rats. In addition, DA5-CH decreased TNFα, IL-1β and IL-6 levels in cortical tissue of HI neonatal rats and in microglia cells subjected to OGD. Moreover, DA5-CH treated microglia medium increased the cell viability, but decreased apoptosis of cortical neurons. DA5-CH suppressed NLRP3 inflammasome activation through inactivation of the TLR2/NF-κB signalling pathway. Furthermore, the protective effects of DA5-CH on the hypoxic-ischemic brain injury were antagonized by nigericin (an NLRP3 agonist). Taken together, our findings revealed that DA5-CH alleviates neonatal hypoxic-ischemic encephalopathy by inhibiting TLR2/NF-κB/NLRP3 mediated-neuroinflammation.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAD (Cath. a-Differentiated) Cells Produce Dopamine along with Dopamine-Synthesizing Enzymes CAD(导管。分化细胞与多巴胺合成酶一起产生多巴胺。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-16 DOI: 10.1007/s11064-025-04474-z

Sanghoon Kim, Edward Pajarillo, Alexis Digman, Itunu Ajayi, Deok-Soo Son, Michael Aschner, Eunsook Lee

{"title":"CAD (Cath. a-Differentiated) Cells Produce Dopamine along with Dopamine-Synthesizing Enzymes","authors":"Sanghoon Kim, Edward Pajarillo, Alexis Digman, Itunu Ajayi, Deok-Soo Son, Michael Aschner, Eunsook Lee","doi":"10.1007/s11064-025-04474-z","DOIUrl":"10.1007/s11064-025-04474-z","url":null,"abstract":"<div><p>Brain dopaminergic (DAergic) neurons play a critical role in mediating motor, reward, and cognitive processes, but are particularly vulnerable to toxic insults associated with Parkinson’s disease and manganism. Several cell lines have been used to study DAergic function and toxicity, and each has distinct advantages and limitations. Here, we investigated whether cath. a-differentiated (CAD) cells, a mouse-derived catecholaminergic cell line, are suitable for DAergic neurotoxicity research as an in vitro model, focusing on their ability to synthesize dopamine (DA) and the expression of key associated proteins. Manganese (Mn) was also tested to determine its DAergic toxicity potential. CAD cells were differentiated with serum deprivation. High-performance liquid chromatography, western blotting, and RT-qPCR were used to assess DA levels, and the expressions of DAergic proteins such as tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AAAD), vesicular monoamine transporter-2 (VMAT-2), and DA transporter (DAT). The results showed that differentiated CAD cells had higher DA levels compared to undifferentiated cells. L-3,4-dihydroxyphenylalanine (L-DOPA), the DA precursor, increased DA production, while carbidopa, an AAAD inhibitor, decreased its production. CAD cells also expressed AAAD protein, indicating that the latter participates in DA synthesis in this cell line. Moreover, Mn decreased DA as well as mRNA and protein levels of DA synthesizing enzymes, such as TH and AAAD, and VMAT-2, thus impairing the DAergic system. Taken together, differentiated CAD cells possess the capability to synthesize DA and express DA-synthesizing enzymes. In addition, Mn caused DAergic toxicity in CAD cells, suggesting that CAD cells are suitable for studying DAergic neurotoxicity.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Dynamin-2 Gene Modified OECs on Functional and Molecular Neuroprotection After Spinal Cord Injury in Rats 动力蛋白-2基因修饰oec对大鼠脊髓损伤后神经功能和分子保护的影响。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-16 DOI: 10.1007/s11064-025-04491-y

Zhijie Yin, Zhiyong Zhang, Haiming Li, Xingping Zhang, Baozhe Jin

{"title":"Effect of Dynamin-2 Gene Modified OECs on Functional and Molecular Neuroprotection After Spinal Cord Injury in Rats","authors":"Zhijie Yin, Zhiyong Zhang, Haiming Li, Xingping Zhang, Baozhe Jin","doi":"10.1007/s11064-025-04491-y","DOIUrl":"10.1007/s11064-025-04491-y","url":null,"abstract":"<div><p>Spinal cord injury (SCI) imposes a significant economic burden on individuals and society, with limited options for repairing the central nervous system after injury. Cell transplantation therapy, particularly using olfactory ensheathing cells (OECs), has shown promise. However, the neuroprotective mechanisms underlying genetically modified OECs remain unclear. This study aimed to investigate the effects of Dynamin-2 (Dnm2) gene-modified OECs (Dnm2-OECs) on SCI repair in a rat model. OECs were harvested from three-day-old rats. Adult rats were divided into sham, SCI, SCI-OEC, and SCI-Dnm2-OECs groups. Dnm2-OECs were transplanted one day post-SCI model, and recovery was assessed through BBB scores, histological analyses, and molecular markers of inflammation. Results showed that the purity and infection efficiency of OECs were 87.28 ± 7.65% and 89.04 ± 5.56%, respectively. Dnm2-OECs survived for at least 28 days post-transplantation and significantly improved BBB scores by day 21. Additionally, neuronal counts increased, syringomyelia decreased, demyelination lessened, and mitochondrial structure improved. Dnm2 levels rose in spinal tissue, while NF-κB pathway proteins and pro-inflammatory cytokines (TRAF6, IL-17, IL-1β and TNF-α) were reduced. Microglial cells (Iba1) decreased correspondingly. These results indicate that Dnm2-OECs transplantation enhances neurological recovery after by inhibiting the NF-κB signaling pathway, offering a potential therapeutic strategy for SCI repair.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

When the Stars Misfire: Astrocytic Dysfunctions in Major Depressive Disorder 当星星失火：星形细胞功能障碍在重度抑郁症。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-12 DOI: 10.1007/s11064-025-04483-y

Candela González-Arias, Gertrudis Perea

引用次数: 0

Combined Chronic Oral Methylphenidate and Fluoxetine Decreases D2R Levels in the Caudate Putamen and Nucleus Accumbens 慢性口服哌甲酯和氟西汀联合降低尾状壳核和伏隔核中的D2R水平。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-11 DOI: 10.1007/s11064-025-04481-0

George Lagamjis, Huy Lu, Nicole M Roeder, Brittany J Richardson, Matthew Marion, Teresa Quattrin, Lucy D. Mastrandrea, Michael Hadjiargyrou, David E. Komatsu, Panayotis K. Thanos

{"title":"Combined Chronic Oral Methylphenidate and Fluoxetine Decreases D2R Levels in the Caudate Putamen and Nucleus Accumbens","authors":"George Lagamjis, Huy Lu, Nicole M Roeder, Brittany J Richardson, Matthew Marion, Teresa Quattrin, Lucy D. Mastrandrea, Michael Hadjiargyrou, David E. Komatsu, Panayotis K. Thanos","doi":"10.1007/s11064-025-04481-0","DOIUrl":"10.1007/s11064-025-04481-0","url":null,"abstract":"<div><p>Methylphenidate (MP) is a commonly prescribed psychostimulant for treating Attention-Deficit/Hyperactive Disorder (ADHD). Many patients with ADHD also experience anxiety and depression, often leading to co-dosing with selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine (FLX), commonly used for ADHD-related and adolescent depression. Our laboratory and others have shown that MP increases striatal dopamine (DA) transporters and DA type 1 receptor binding (D1R) in rats, and FLX has been shown to affect the DA reward pathway through the effect DA receptors play on increased cellular serotonin (5-HT). However, the effects of combined MP and FLX on DA receptor binding remain unclear. This study investigated how MP, FLX, and their combination affect D1R and DA type 2 (D2R) binding. At three weeks of age, adolescent rats received four weeks of oral drug treatments via a previously established dosing paradigm that replicates human pharmacokinetics. Rats were separated into four groups, receiving water, MP, FLX, or MP + FLX. Following treatment, autoradiography binding was conducted on coronal brain sections and showed chronic combined treatment with MP + FLX resulted in significant decreases in D2R levels relative to controls in the: Dorsal Caudate Putamen (DCPU) (51.5%), Dorsolateral Caudate Putamen (DLCPU) (50.4%), Nucleus Accumbens Core (Nac Core) (44.8%), Ventral Caudate Putamen (VCPU) (47.7%), and Ventromedial Caudate Putamen (VMCPU) (49.1%). No significant effects were reported for D1R binding. Thus, the combined treatment of MP + FLX in attenuating D2R levels may be involved in the mechanism that prior literature has described an increased risk for substance use disorder, cognitive deficits and motor dysregulation.</p></div>","PeriodicalId":719,"journal":{"name":"Neurochemical Research","volume":"50 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Graptophyllum Grandulosum Prevents Nicotine Addiction in Pubescent Adolescent Rats: Evidence on Dopaminergic, Cholinergic, BDNF and Neuroinflammatory Pathways 更正：大叶葡萄防止青春期大鼠尼古丁成瘾：多巴胺能、胆碱能、BDNF和神经炎症途径的证据。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-10 DOI: 10.1007/s11064-025-04477-w

Steve Brunel Kenfack Ngoufack, Gwladys Temkou Ngoupaye, Nadège Emégam Kouémou, Jospin Chirac Noubouwo, Aurelien Fossueh Foutsop

引用次数: 0

Correction: Mechanisms of Fat Mass and Obesity-Associated Protein in Regulating Cognitive Impairment Induced by Sevoflurane Anesthesia in Neonatal Rats 修正：脂肪量和肥胖相关蛋白调节七氟醚麻醉致新生大鼠认知功能障碍的机制。

IF 3.8 3区医学

Neurochemical Research Pub Date : 2025-07-10 DOI: 10.1007/s11064-025-04482-z

Chan Shen, Yanghongyun Guo, Jun Zheng, Jiayu Wang, Liangcheng Zhang

引用次数: 0