Acta Facultatis Medicae Naissensis最新文献

{"title":"Enlarged perforating flaps of the anterior thoracic and abdominal wall: A novel approach to autologous breast reconstruction","authors":"Feyta Ruslanovych, Zhernov Andriyovych, Zhernov Oleksandrovych, Kozinets Pavlovych","doi":"10.5937/afmnai39-33865","DOIUrl":"https://doi.org/10.5937/afmnai39-33865","url":null,"abstract":"Aim. To develop methods for obtaining enlarged perforating flaps on the anterior surfaces of the thoracic and abdominal walls. Material and methods: The study included 16 patients with post-burn breast deformities aged from 5 to 22 years. In the upper part of the anterior surface of the chest, the flaps were formed on the basis of 2, 3 anterior intercostal artery perforator and thoracoacromial artery perforator with the inclusion of the vascular network of the thoracic branch of the supraclavicular artery. In the lower part of the thoracic and upper abdominal wall, there were also enlarged flaps based on the superior epigastric artery perforator with the connection of vascular networks 6, 7 anterior intercostal artery perforator and perforator deep inferior epigastric artery. The obtained flaps were moved by direct advancement, rotation and transposition methods. Results: The largest area of the wound defect was closed with flaps that were moved by rotation and it was 1.8 times larger than the area of wounds closed by direct advancement and 1.3 times larger than if using transposition flaps. The use of stretched perforating flaps from the anterior surface of the thoracic and abdominal walls allowed to obtain a positive result in 90.4% of cases with the short-term treatment, and in all cases with the long-term treatment. Conclusion: A simple technique based on the proposed methods can be widely used for the reconstruction of the upper and lower quadrants of the breast and intermammary and submammary spaces.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analysis of laboratory parameters of chronic kidney failure in elderly patients","authors":"E. Hasković, Harun Uzunalıć, S. Unčanin, S. Herenda, Denis Hasković, A. Selimović","doi":"10.5937/afmnai39-34954","DOIUrl":"https://doi.org/10.5937/afmnai39-34954","url":null,"abstract":"Aim: The chief aim of this study was monitoring of laboratory parameters of chronic kidney failure in elderly patients. Methods: All samples were taken and processed by standard methods and according to the principles of good laboratory practice. Data were collected in an organized and systematic manner in the form of a questionnaire with respect to ethical principles and as such were analyzed by statistical tests and analyses (Student's t-test, Analysis of variance-ANOVA, Pearson's and Spearman's correlation coefficients). The limit of statistical significance was set at p < 0.05. Results: Mean values of creatinine clearance and proteinuria for the total study population were: 41.30 ± 21.43 mL/min, 1.5 ± 2.3 g/L/24 h, respectively. Hematological parameters did not significantly differ from normal values. The highest frequency of comorbidities was observed in subjects aged ≥ 80 years with an average of 2.03 comorbidities per subject. Serum creatinine and urea values as well as creatinine clearance are good indicators of disease progression. Conclusion: The results of the presented research suggest that old age is a predisposing risk factor for the development of chronic kidney disease, and that in combination with comorbidities (hypertension and/or diabetes), it contributes to poor prognosis or disease progression.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mature gastric teratoma in a female infant associated with malrotation: A case report","authors":"Maja Zecevic, Z. Marjanovic, D. Živanović, M. Slavković","doi":"10.5937/afmnai39-31345","DOIUrl":"https://doi.org/10.5937/afmnai39-31345","url":null,"abstract":"Gastric teratoma is a extremely infrequent tumor and accounts for less than 1% of all teratomas among children population with male predominance. Case history: Herewith is described a case of mature gastric teratoma in a three months-old female infant associated with malrotation, hypotonia, torticollis and fusion of labia minoria. Due to the abdominal distension, the child was admitted to the Paediatric Surgery Department. On physical examination, a large lump was palpable, and laboratory findings were normal. Computed tomography (CT) showed a major heterogeneous solid formation within the right hemi-abdomen, extending to pelvis and crossing the midline. The infant underwent surgery and complete excision of tumor originating from lesser curvature of the stomach was made. Tumor was weighing 600 gr and was 10 x 14 x 8 cm large. The histopathological examination finding pointed to mature gastric teratoma. The post-operative course and further follow-up were eventless. Conclusion: As soon as the diagnosis is made, the surgery is of paramount importance, especially in cases of immature teratomas, where malignant alteration is possible.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrocytoprotective properties of cryopreserved placenta extract in combined action of low temperatures and inhibition of cyclooxygenase","authors":"F. Hladkykh","doi":"10.5937/afmnai39-33036","DOIUrl":"https://doi.org/10.5937/afmnai39-33036","url":null,"abstract":"Introduction. Non-steroidal anti-inflammatory drugs (NSAIDs) are ranked first among the world's effective anti-inflammatory and analgesic drugs with anticipated side effects. That is why the prevention of development of adverse reactions associated with NSAIDs, in particular, of those related to their ulcerogenicity, remains a serious global problem. Aims. To characterize the mechanisms of gastrocytoprotective activity of cryopreserved placenta in combined action of low temperatures and diclofenac sodium. Material and methods. The study was performed on 42 male rats weighing 200-220 g. Acute diclofenac sodium-induced gastropathy was reproduced by a single injection of the latter in rats at the dose of 50 mg/kg. The content of malonic dialdehyde, catalase activity, prostaglandin synthase activity and the content of nitrogen monoxide metabolites in homogenates of gastric mucosa were determined by spectrophotometric method. Results and discussion. The study showed that prophylactic administration of placental cryoextract in rats with diclofenac sodium-induced gastropathy is associated with increased activity of antioxidant system in gastric mucosa as demonstrated by an elevated catalase activity by 40.0% as compared with control rats. Modulation of antioxidant-prooxidant homeostasis is believed to be one of the principal mechanisms of gastrocytoprotective action in combined action of low temperatures and cryoextract of the placenta. This is shown by a statistically significant (p < 0.05) 2.2-fold increase of antioxidant-prooxidant index in the study group as compared with rats with diclofenac sodium-induced gastropathy. Administration of placental cryoextract was found to increase prostaglandin synthase activity in rats with diclofenac sodium-induced gastropathy by two times as compared with control rats, which would reduce iatrogenic prostaglandin deficiency in gastric mucosa. Also, the combined action of low temperatures and of placenta cryoextract was associated with a statistically significant (p < 0.05) increase in the level of metabolites of nitrogen monoxide (by 70.1%) as compared with rats with diclofenac sodium-induced gastropathy. Conclusions. Modulation of prooxidant-antioxidant homeostasis in gastric mucosa and increase in contents of nitrogen monoxide metabolites and prostaglandin synthase activity are the leading mechanisms of gastroprotective activity of cryopreserved placental extract in diclofenac sodium-induced gastropathy.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cipargamin could inhibit human adenosine receptor A3 with higher binding affinity than Plasmodium falciparum P-type ATPase 4: An In silico study","authors":"T. Fatoki, Oladoja A. Awofisayo, B. Faleye","doi":"10.5937/afmnai39-31499","DOIUrl":"https://doi.org/10.5937/afmnai39-31499","url":null,"abstract":"Aim: This study aimed to predict the molecular targets of cipargamin in humans and estimate the structural dynamics and binding affinity of their interactions compared to that of Plasmodium falciparum P-type ATPase 4 (PfATP4). Methods: In silico methods were used in this study which include target prediction, structure modeling and dynamics, and molecular docking. Results: The results showed that cipargamin had 100% probability of binding to the human adenosine A3 receptor (ADORA3) and about 15% for other human targets which include tyrosine-protein kinase JAK2, adenosine A2a receptor, phosphodiesterase 5A and cathepsin K. The results of molecular docking showed that binding energy of cipargamin to PfATP4 and hADORA3 were-12.40 kcal/mol-1 and-13.40 kcal/mol-1 respectively. The docking was validated by the binding of enprofylline and fostamatinib to PfATP4 and hADORA3. Overall, the binding of cipargamin was closely similar to that of fostamatinib. This study shows the potential of cipargamin to modulate the activities of PfATP4 of the parasite (P. falciparum) as well as ADORA3 of the host (Homo sapiens). Conclusion: All the previous studies of cirpagamin have not implicated its action on hADORA3, thus this study provides an insight into a possible role of hADORA3 in the mechanism of malarial infection.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and simple laboratory data associated with fatal outcomes in patients with acute pancreatitis","authors":"T. Formanchuk, V. Shaprinskiy, A. Formanchuk","doi":"10.5937/afmnai39-32308","DOIUrl":"https://doi.org/10.5937/afmnai39-32308","url":null,"abstract":"Aims. The aim of the study was to evaluate the association of mortality in acute pancreatitis with clinical and simple laboratory data received on the day of admission. Patients and methods. In our retrospective study, the clinical and laboratory parameters of 99 patients with moderate and severe acute pancreatitis were analyzed. All patients were divided into two groups: deceased and survivors. Results. We did not find a significant difference in age and gender distribution between the comparison groups. However, a significant predominance of alcoholic etiology of acute pancreatitis, early hospitalization (up to 6 hours from the onset of the disease) of patients, and the number of necrotizing infected type in the deceased group were found. Concomitant pathology did not significantly differ in comparison groups. In patients from the deceased group, the total number of all complications was significantly higher than in the group of survivors-21 (100%) and 42 (53.8%) (p = 0.0001), respecting. Among the laboratory parameters determined on the day of admission, in the deceased group, there was a significant increase in stabs to 19.8 ± 9.8 and ESR, AST to 225.3 ± 47.5 U/L, urea to 11.2 ± 7.7 mmol/L, and creatinine to 173.6 ± 26.1 mmol/L. Conclusion. The alcoholic genesis of acute pancreatitis, necrotizing infected type of inflammation of the pancreas, presence of late complications, and comorbidities were significantly higher in the deceased group. The levels of stabs, ESR, AST, urea, and creatinine determined on the admission significantly dominated in the deceased group, which requires further study for the prediction of mortality of acute pancreatitis.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid: A case report","authors":"Dejan Dobrijević, Nataša Kaćanski-Rađenović, Jasmina Katanić, J. Kolarovic","doi":"10.5937/afmnai39-35735","DOIUrl":"https://doi.org/10.5937/afmnai39-35735","url":null,"abstract":"Introduction: Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid is a rare phenomenon caused by autoantibodies against platelet membrane with overall prevalence of 0.1% in the general population. This phenomenon is an in vitro artifact and with different anticoagulant used, the patient had the physiological platelet count. Case report: This case is a report of a 2-year-old patient, whose initial complete blood count analysis revealed low platelet count (18 x 10 9 /L) without any visible signs of hemorrhage. After detecting multiple platelet clumps in the peripheral blood smear, the complete blood count was repeated using 3.2% sodium-citrate. The analysis of the new specimen revealed the physiological platelet count. Thus, the patient was diagnosed with pseudothrombocytopenia induced by ethylenediaminetetraacetic acid. Conclusion: Pseudothrombocytopenia induced by ethylenediaminetetraacetic acid should always be suspected whenever thrombocytopenia without bleeding tendency is presented. The peripheral blood smear examination is the most convenient and the cheapest diagnostic test for excluding this artifact.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sanders type III calcaneal fracture fixed with a locking angular plate: A case report","authors":"M. Mladenović, S. Milenkovic, P. Stojiljković, Andrija Krstić","doi":"10.5937/afmnai39-33264","DOIUrl":"https://doi.org/10.5937/afmnai39-33264","url":null,"abstract":"Introduction. The calcaneus is a tarsal bone which plays a major role in transferring weight from the lower leg down through the ankle joint onto the forefoot and toes. It participates in the formation of the outer and inner longitudinal arch of the foot and has a significant role in walking and transferring body weight. Calcaneal fractures most often occur by jumping, falling from a height, or less often, in traffic accidents. Calcaneal fractures can be extra-articular and intra-articular. Intra-articular fractures can entirely damage joint surfaces, which results in deformity and immobility of the foot. They can be extra-articular and intraarticular, when disruption of the entire articular surfaces, deformity, and weakness of the foot occur. Case report. This case report presents a 40-year-old male with an intra-articular calcaneal fracture. He was injured by falling from a height of 3 meters and sustained an intra-articular fracture type IIIAB where one fracture line went laterally and the other centrally over the posterior calcaneal facet. Surgery was performed on the sixth day after the injury. The prepared locking plate for calcaneus was adapted and fixed with spongy screws in relatively preserved joint fragments: sustentaculum tali, tuberositas lateralis calcanei and tuber calcanei. In this way, we ensured the position of the repaired fragments, and then we placed 4 more spongy screws inside the healthy bone tissue, which was enabled with the use of this plate. With this procedure, the calcaneal axis, i.e. the varus deformity, height, width, length and angles of the bone (Bohler's and Gissane's angle) were corrected. From day one, the patient started to move his toes, and on the third day the patient started to move the ankle and began to walk with the help of props with no support on the leg. Conclusion. The preoperative value of Bohler's angle is a significant correlation between the severity of the injury and displacement of fragments in intra-articular calcaneal fractures. The goal of the surgery was to restore the posterior calcaneal articular facet, avoid soft tissue infection and form a normal shape and position of a foot.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast milk components and factors that may affect lactation success","authors":"Jelena Miolski, M. Raus, Milica Radusinović, V. Zdravković","doi":"10.5937/afmnai39-31436","DOIUrl":"https://doi.org/10.5937/afmnai39-31436","url":null,"abstract":"Introduction. Breastfeeding is the most important way to feed a newborn, infant and a young child. Lactogenesis is the ability of secreting milk from the mammary gland and includes secretory initiation and activation. The birth of the placenta and decrease in progesterone levels stimulate an increase in prolactin levels and the secretion of milk from the breast. Human milk contains carbohydrates, lipids, proteins, vitamins, minerals, fatty acids, amino acids and trace elements. In addition, numerous cells, macrophages, bacteria, chemokines, cytokines, immunoglobulins, hormones, growth factors, and mucin are present. The composition of milk differs depending on the phase of secretion. Colostrum is secreted for the first five days after birth, then comes transitional milk, and mature milk two weeks after birth. During the very act of breastfeeding, there is a difference between pre-milk and last milk. In pre-milk, the fat content is low and increases with the duration of lactation, and in breast milk it is relatively high. Aim: To point out the importance of milk composition and factors that are influential for breastfeeding. Conclusion: Maternal parity, age or socioeconomic status and education can affect the concentration of certain components of breast milk. Adequate social policy and support, along with education related to early breastfeeding experiences, can be the key factors in initiating and maintaining breastfeeding. Besides the mother and the child, the entire social community would benefit from that.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation of biologics for alternative routes of administration: Current problems and perspectives","authors":"S. Stankovic, M. Tasić-Kostov","doi":"10.5937/afmnai39-35426","DOIUrl":"https://doi.org/10.5937/afmnai39-35426","url":null,"abstract":"Introduction: Biologics (biopharmaceuticals) present new promising therapies for many diseases such as cancers, chronical inflammatory diseases and today's biggest challenge - COVID-19. Research: Today, most biologics have been synthetized using modern methods of biotechnology, in particular DNA recombinant technology. Current pharmaceutical forms of protein/peptide biopharmaceuticals are intended for parenteral route of administration due to their instability and large size of molecules. In order to improve patient compliance, many companies are working on developing adequate forms of biopharmaceuticals for alternative, non-invasive routes of administration. The aim of this work is to review current aspirations and problems in formulation of biopharmaceuticals for alternative (non-parenteral) routes of administration and to review the attempts to overcome them. These alternative routes of administration could be promising in prevention and treatment of COVID-19, among other serious diseases. Conclusion: The emphasis is on stabilizing monoclonal antibodies into special formulations and delivery systems; their application should be safer, more comfortable and reliable. When it comes to hormones, vaccines and smaller peptides, some companies have already registered drugs intended for nasal and oral delivery.","PeriodicalId":7132,"journal":{"name":"Acta Facultatis Medicae Naissensis","volume":"1 1","pages":""},"PeriodicalIF":0.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71195593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}