低温和抑制环氧化酶联合作用下冷冻胎盘提取物的胃细胞保护作用

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL
F. Hladkykh
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引用次数: 17

摘要

介绍。非甾体抗炎药(NSAIDs)是世界上最有效的抗炎镇痛药物,其副作用是可以预见的。这就是为什么预防与非甾体抗炎药相关的不良反应,特别是与其致溃疡性相关的不良反应,仍然是一个严重的全球问题。目标探讨低温与双氯芬酸钠联合作用下低温保存胎盘胃细胞保护作用的机制。材料和方法。研究对象为42只体重200-220克的雄性大鼠。双氯芬酸钠单次注射50 mg/kg双氯芬酸钠可引起大鼠急性胃病。采用分光光度法测定胃黏膜匀浆中丙二醛含量、过氧化氢酶活性、前列腺素合成酶活性及一氧化氮代谢物含量。结果和讨论。研究表明,双氯芬酸钠诱导的胃病大鼠预防性给予胎盘冷冻提取物与胃黏膜抗氧化系统活性增加有关,过氧化氢酶活性比对照大鼠提高了40.0%。在低温和胎盘冷冻提取物联合作用下,抗氧化-促氧化平衡的调节被认为是胃细胞保护作用的主要机制之一。与双氯芬酸钠诱导的胃病大鼠相比,研究组抗氧化-促氧化指数增加2.2倍,具有统计学意义(p < 0.05)。胎盘冷冻提取物可使双氯芬酸钠诱导的胃病大鼠前列腺素合成酶活性提高2倍,从而减轻医源性胃粘膜前列腺素缺乏。此外,与双氯芬酸钠诱发胃病的大鼠相比,低温和胎盘冷冻提取物的联合作用与一氧化氮代谢物水平增加(70.1%)有统计学意义(p < 0.05)。结论。调节胃粘膜促氧化-抗氧化稳态、增加一氧化氮代谢物含量和前列腺素合成酶活性是低温保存胎盘提取物对双氯芬酸钠诱发胃病胃保护作用的主要机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gastrocytoprotective properties of cryopreserved placenta extract in combined action of low temperatures and inhibition of cyclooxygenase
Introduction. Non-steroidal anti-inflammatory drugs (NSAIDs) are ranked first among the world's effective anti-inflammatory and analgesic drugs with anticipated side effects. That is why the prevention of development of adverse reactions associated with NSAIDs, in particular, of those related to their ulcerogenicity, remains a serious global problem. Aims. To characterize the mechanisms of gastrocytoprotective activity of cryopreserved placenta in combined action of low temperatures and diclofenac sodium. Material and methods. The study was performed on 42 male rats weighing 200-220 g. Acute diclofenac sodium-induced gastropathy was reproduced by a single injection of the latter in rats at the dose of 50 mg/kg. The content of malonic dialdehyde, catalase activity, prostaglandin synthase activity and the content of nitrogen monoxide metabolites in homogenates of gastric mucosa were determined by spectrophotometric method. Results and discussion. The study showed that prophylactic administration of placental cryoextract in rats with diclofenac sodium-induced gastropathy is associated with increased activity of antioxidant system in gastric mucosa as demonstrated by an elevated catalase activity by 40.0% as compared with control rats. Modulation of antioxidant-prooxidant homeostasis is believed to be one of the principal mechanisms of gastrocytoprotective action in combined action of low temperatures and cryoextract of the placenta. This is shown by a statistically significant (p < 0.05) 2.2-fold increase of antioxidant-prooxidant index in the study group as compared with rats with diclofenac sodium-induced gastropathy. Administration of placental cryoextract was found to increase prostaglandin synthase activity in rats with diclofenac sodium-induced gastropathy by two times as compared with control rats, which would reduce iatrogenic prostaglandin deficiency in gastric mucosa. Also, the combined action of low temperatures and of placenta cryoextract was associated with a statistically significant (p < 0.05) increase in the level of metabolites of nitrogen monoxide (by 70.1%) as compared with rats with diclofenac sodium-induced gastropathy. Conclusions. Modulation of prooxidant-antioxidant homeostasis in gastric mucosa and increase in contents of nitrogen monoxide metabolites and prostaglandin synthase activity are the leading mechanisms of gastroprotective activity of cryopreserved placental extract in diclofenac sodium-induced gastropathy.
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来源期刊
Acta Facultatis Medicae Naissensis
Acta Facultatis Medicae Naissensis MEDICINE, GENERAL & INTERNAL-
CiteScore
0.70
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0.00%
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13
审稿时长
12 weeks
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