中华实验和临床病毒学杂志最新文献

{"title":"[Quantitative detection of serum procollagen III N-terminal peptide chemiluminescence enzyme immunoassay].","authors":"Jia Liu,&nbsp;Yan-Qing Feng,&nbsp;Yong-Ji Song,&nbsp;Jun Hou,&nbsp;Lin Chen,&nbsp;Jing Zhao,&nbsp;Ai-Xia Liu,&nbsp;Jing-Xia Guo,&nbsp;Jun Xu,&nbsp;Hong-Shan Wei,&nbsp;Bo-An Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To establish chemiluminescence enzyme immunoassay (CLEIA) for quantitative detection of procollagen III N-terminal peptide (P III NP) in serum.</p><p><strong>Methods: </strong>A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of P III NP as the catalytic enzyme and the luminol as the luminescence reagent. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as linear range, sensitivity, specificity, stability and so on. The CLEIA was compared with imported ELISA kits, by detecting clinical serum.</p><p><strong>Results: </strong>The linear range was 0.8-85 ng/ml. The detection limit was 0.5 ng/ml. Inter-assay and intra-assay RSD were both less than 10%. The recoveries of three different spiked concentration samples were 96.2%, 91.2% and 101.1%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.99 and RSD lower than 6%. The detected results of clinical sera with CLEIA closely corresponded to those with imported ELISA.</p><p><strong>Conclusion: </strong>Established CLEIA for quantity determination of serum P III NP has high accuracy, sensitivity and repeatability.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 5","pages":"385-7"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32190333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analyse related factors of impact and prognosis of 73 cases of severe hepatitis].","authors":"Jun-Mei Zhao,&nbsp;Lu Zhang,&nbsp;Qing-Wei Du,&nbsp;Cai-Qin Mu,&nbsp;Yv-Lian Ren,&nbsp;Lei-Ping Hu,&nbsp;Ge Shen,&nbsp;Li-Wei Zhuang,&nbsp;Yao Lu,&nbsp;Guo-Hua Qiu,&nbsp;Qing-Feng Sun,&nbsp;Yun-Zhong Wu,&nbsp;Min Yang,&nbsp;Ming-Hui Li,&nbsp;Yao Xie,&nbsp;Jun Cheng,&nbsp;Dao-Zhen Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>A retrospective study was conducted to investigate the clinical features and prognostic factors of 73 cases of severe hepatitis.</p><p><strong>Methods: </strong>To summarize clinical features of 73 cases of severe hepatitis, grouping by etiology and pathogenesis. A retrospective analysis was performed to evaluate the relationship between biochemical characteristics (liver function, renal function, electrolytes, PTA, etc) and complications (hepatic encephalopathy, upper gastrointestinal bleeding, hepatorenal syndrome, ascites, abdominal infections, etc) and prognosis.</p><p><strong>Results: </strong>(1) HBV infection alone accounted for 65.75%. Alcoholic liver disease, drug-induced liver injury, hepatitis E, autoimmune hepatitis, overlapping causes and other factors were five cases (6.85%), six cases (8.22%), two cases (2.74%), two cases (2.74%), seven cases (9.59%) and three cases (4.11%) respectively. According to the incidence rate, severity and underlying liver condition, subacute hepatitis, cases based on chronic hepatitis and on cirrhosis were 12 cases (16.43%), 11 cases (15.07%), 50 cases (68.49%) respectively. Clinical manifestations with or without hepatic encephalopathy accounted for 58.90% or 41.10%. (2) The highest mortality of severe hepatitis was alcoholic liver disease and patients on the basis of overlapping factors (66.67%), followed by autoimmune liver disease (50%). The mortality of HBV-related hepatitis was 18.75%. Overall mortality of 73 cases of severe hepatitis was 28.77%, of which cirrhosis group was higher than non-cirrhotic group (40% vs 4.3%, P = 0.002). The difference was statistically significant. Patients without hepatic encephalopathy had lower mortality than with hepatic encephalopathy (3.33% vs 46.51%). The mortality of patients with hepatic encephalopathy Stage III and IV was 72.73%. (3) Independent samples t test filtered nine factors associated with death, namely cirrhosis, upper gastrointestinal bleeding, hepatic encephalopathy, hepatorenal syndrome, serum creatinine, total bilirubin (TBIL), direct bilirubin (DBIL), albumin (ALB) and serum sodium. The results of multivariate conditional logistic regression analysis indicated that hepatic encephalopathy, serum creatinine levels were risk factors for death, whereas ALB as a protective factor.</p><p><strong>Conclusion: </strong>Hepatic encephalopathy, serum creatinine levels were risk factors for severe hepatitis death, But ALB was protective factor. Nucleotide analogs using was the main reason why the mortality of hepatitis B was as low as 18.75%.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 5","pages":"366-9"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32187774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Model building-up and observation on the mouse carried chronic hepatitis B and nonalcoholic fatty liver disease].","authors":"Lu Lu,&nbsp;Yin-Lan Liu,&nbsp;Wen-Jun Yang,&nbsp;Jing Liu,&nbsp;Yan Luo,&nbsp;Zhen-Jie Zhuang,&nbsp;Jian-Yu Chen,&nbsp;Dong-Xue Bian,&nbsp;Yun-Hao Xun,&nbsp;Jun-Ping Shi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Establish the model of mouse with chronic hepatitis B virus (HBV) and nonalcoholic fatty liver disease (NAFLD).</p><p><strong>Methods: </strong>Take 100 HBV transgenic, BALB/c mice of 4 weeks old, with each gender half. Then pick out 70 mice in one group to feed high-fat feed and the rest to feed normal feed. At the end of week 16, random kill 10 mice of high-fat, then liver tissue and serological detection target identification model is established in this paper. After that, divide the mice into model group and comparison group with 30 mice in each group. Feed model group with high-fat feed, comparison group with normal feed and normal group with normal feed till week 72 (including previous 16 weeks). Kill 10 mice of each group at the end of week 24, 48 and 72 respectively, fully automatic biochemical instrument detection of serum ALT, AST, TC, TG, FBG, fluorescence quantitative PCR method to detect HBV-DNA, chemiluminescence detection of HBsAg, liver biopsy after HE staining to evaluate histology change, observe mice model of dynamic evolution.</p><p><strong>Results: </strong>(1) Feed high fat feed after 16 weeks, mice's weight, serum ALT, AST, TC, TG, FBG and blood biochemical indicators increased, HBV-DNA positive, liver HE staining obviously big blister fatty degeneration of liver cells and within the lobule lymphocytes infiltration, NAFLD activity score (NAS) getting close to NASH, the model of chronic HBV carries with NAFLD mouse built successfully. (2) The TC and TG values of model group in each period were higher than that of comparison group and normal group. (3) In week 24 and 72, HBV-DNA values of each group are obvious different from the other two groups and the difference can be applied to statistical significance (P < 0.05). (4) In week 48 and 72, NAS of each group are obvious different from the other two groups and the difference can be applied to statistical significance (P < 0.05).</p><p><strong>Conclusions: </strong>(1) Chronic HBV carries with NAFLD mice model can be established by HBV transgenic mice fed by high fat feed. (2) NAFLD accelerates the liver disease of the mice carrying HBV to some extent.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 5","pages":"332-5"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32188340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification and characterization of enterovirus 71 isolated in Yunnan province].","authors":"Shi-Wen Zhao,&nbsp;Wei-Dong Yin,&nbsp;Jian-Ping Cun,&nbsp;Qiang Gao,&nbsp;Jie Yin,&nbsp;Guo-Jun Liu,&nbsp;Wen Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.</p><p><strong>Methods: </strong>Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.</p><p><strong>Result: </strong>All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.</p><p><strong>Conclusion: </strong>Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"263-5"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparative study on the clinical characteristics of HBV infection patients with different pathologic inflammation grade].","authors":"Can-Hui Xiao,&nbsp;Hai-Xia Sun,&nbsp;Ka Zhang,&nbsp;Xing-Fei Pan,&nbsp;Fei-Fei Huang,&nbsp;Qi-Huan Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to compare the biochemical and virological characteristics among patients infected with hepatitis B virus (HBV) according to pathologic inflammation grade.</p><p><strong>Methods: </strong>428 patients with chronic HBV infection accept liver biopsy, liver function test, HBeAg detection and HBV DNA levels detection. They were studied and subdivided into four groups according to pathologic inflammation grade. The biochemical and virological characteristics were studied. Univariate analysis was performed with the SPSS 16.0.</p><p><strong>Results: </strong>In different inflammation grading group, mean age and sex composition were no difference. Serum levels of ALT was highest in group G3 and lowet in group G0-1, there was statistically significant among groups (P = 0.005); AST and TBil were all highest in group G4 and lowest in group G0-1, statistically significant also found among groups (P = 0.000 & 0.004). Serum levels of ALB and PTA were all highest in group G0-1 and lowest in group G4, had statistically significant among groups (P = 0.000 & 0.000). There was no difference of HBV DNA level and percentage of HBeAg (+) among four groups (P = 0.565 & 0.065).</p><p><strong>Conclusions: </strong>The serum AST, TBil, ALB and PTA were different and can partly reflect the inflammation degree of liver damage in patients with HBV infection. ALT and PTA can reflect the inflammation degree of G0-1, G2 and G3; AST, TBil, ALB and PTA reflect the G3 and G4. HBV DNA level and HBeAg status can not indicate the inflammation degree in HBV infection patients.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"270-2"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research of the relationship of ubiquinone and beclin-1 and liver mitochondria].","authors":"Fang Xie,&nbsp;Chao Zhang,&nbsp;Yue-Ke Zhu,&nbsp;Hui Liu,&nbsp;Xue-Mei Liu,&nbsp;Lin Jia,&nbsp;Ke-Fei Wang,&nbsp;De-Xi Chen,&nbsp;Qing-Hua Meng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study whether CO-Q10 can protect liver injury caused by acute on chronic liver failure (ACLF) by autophagy.</p><p><strong>Methods: </strong>Rats were separated into three groups: control group, acute on chronic liver failure (ACLF) and intervenient group, liver tissues were observed by optical microscopy and electron microscopy. The levels of Beclin-1 expression were determined by real-time PCR. And Western Blot.</p><p><strong>Results: </strong>Areas of necrosis detected in intervenient group were alleviated than in ACLF significantly. Most mitochondrias had been degradated in ACLF group while alive in intervenient group. Real-time PCR and Western Blot revealed level of beclin-1 in ACLF was lower than control and intervenient group.</p><p><strong>Conclusion: </strong>Intervenient group may ameliorate rat liver injury by promoting autophagy.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"250-2"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular epidemiological study of measles viruses isolated in Qinghai Province during 2000-2011].","authors":"Li-Xia Fan,&nbsp;Zhuo-Ma Ba,&nbsp;Sheng-Cang Zhao,&nbsp;Hu Yi,&nbsp;Shuang-Ying Jiang,&nbsp;Yan Zhang,&nbsp;Hui-Ling Wang,&nbsp;Wen-Bo Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To carry out the molecular epidemiological study of the wild-type measles virus isolated in Qinghai Province during 2000-2011, and provide a scientific basis for the measles elimination.</p><p><strong>Methods: </strong>Measles viruses were isolated using B95a cell line or Vero/SLAM cell line from throat swabs collected from suspected measles cases during measles outbreak and sporadic in 6 prefectures during 2000-2011. The fragment of 696 nucleotides of N gene carboxy terminal was amplified by using RT-PCR methods. The PCR products were sequenced and analyzed. The phylogenetic tree was conducted with the viruses isolated in viruses from other province.</p><p><strong>Results: </strong>Total 19 measles viruses were isolated during 2000-2011 in Qinghai province and all belong to genotype H1a. The results of phylogenetic tree showed that viruses in 2000-2005 and in 2009-2011 were distributed in two different lineages, and it revealed that these strains belonged to at least 2 viral transmission chains and the viruses circulated during 2000-2005 were not detected after 2005.</p><p><strong>Conclusion: </strong>Genotype H1a was the predominant genotype circulated in Qinghai province during 2000-2011. Qinghai measles virus strains had not evolved independently, but coevolved with the measles virus strains in other provinces in mainland China. The variation of important amino acid sites of measles virus should be continuous monitored and provide the scientific strategy for the measles elimination.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"253-6"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effects of recombinant human beta-defensin-3 on expression of interleukin-17A and interleukin-22 in BEAS-2B cell].","authors":"Bing-Ya Guo, Guang-Cheng Xie, Zhao-Jun Duan, Li-Jun Xia","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To research the effects of recombinant human beta-defensin-3 (hBD-3) on expression of interleukin-17A (IL-17A) and interleukin-22 (IL-22) in BEAS-2B cell.</p><p><strong>Methods: </strong>The BEAS-2B cells were stimulated with different concentrations of hBD-3 for 6 hours and 24 hours, respectively. Toll-like receptor 2 (TLR2), IL-17A and IL-22 mRNA expression levels were determined by real-time PCR, and the expression levels of IL-17A and IL-22 protein were examined by enzyme linked immune-sorbent assay.</p><p><strong>Results: </strong>TLR2 mRNA in BEAS-2B cells were significantly increased in a concentration-and time-dependent manner after stimulating by hBD-3 for 24 hours compared to 6 hours. The IL-17A has significantly increased in mRNA and protein levels stimulated 24 hours in a concentration of 100 ng/ml, however, IL-17A mRNA expression has increased while protein didn't change stimulated 6 hours in a concentration of 50 ng/ml. The IL-22 mRNA and protein expression reached peak levels after stimulating in a concentration of 50 ng/ml of hBD-3 while IL-22 expression declined in mRNA and protein levels as the concentration of hBD-3 increased.</p><p><strong>Conclusions: </strong>Recombinant hBD-3 can up-regulated the expression of TLR2, IL-17A and IL-22, lower concentration of hBD-3 mainly increased the expression of IL-22 while higher concentration of hBD-3 mainly increased the expression of IL-17A. These results show that different concentrations of hBD-3 maybe activate different transcription factors which was mediated by TLR2, initiating host immune response.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"260-2"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning and expressing of golgi protein73 gene fragment and preparation of monoclonal antibodies against the recombinant protein].","authors":"Jing-Xia Guo,&nbsp;Hong-Shan Wei,&nbsp;Yong-Ji Song,&nbsp;Jing Zhao,&nbsp;Ai-Xia Liu,&nbsp;Jia Liu,&nbsp;Lin Chen,&nbsp;Jun Xu,&nbsp;Bo-An Li,&nbsp;Yuan-Li Mao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To clone and express human Golgi glycoprotein73 protein, and prepare the monoclonal antibody (mAb) against the protein.</p><p><strong>Methods: </strong>GP73 gene was amplified from HepG2 cells by RT-PCR, then ligated with pQE31 to form recombinant plasmid pQE-GP73 and transformed into E. coli BL21. The protein induced by IPTG was purified by 6 x His-tag and used to immunize the BALB/c mice. The specific monoclonal antibodies (mAbs) were prepared by the cell fusion technique. Western Blot was used to detect specificity of mAbs.</p><p><strong>Results: </strong>The prokaryotic plasmid expressing the recombinant protein was constructed, and the GP73 recombinant protein was expressed and purified. Five hybridoma cell lines that secreted anti-GP73 mAbs were obtained. 2 of 5 mAbs were the IgG1 subtype. Western Blot indicated the mAbs showed specific combination with GP73 protein.</p><p><strong>Conclusion: </strong>The GP73 recombinant protein is highly purified and has strong antigenicity. The anti-GP73 mAbs were prepared successfully.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"301-3"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C].","authors":"Long-You Zhao,&nbsp;Yong-Ping Ji,&nbsp;De-Bin Wang,&nbsp;Jie Zhuang,&nbsp;Bin Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.</p><p><strong>Methods: </strong>A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.</p><p><strong>Results: </strong>To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.</p><p><strong>Conclusions: </strong>The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"304-6"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32161786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}