[重组人 beta-defensin-3 对 BEAS-2B 细胞中白细胞介素-17A 和白细胞介素-22 表达的影响]。

中华实验和临床病毒学杂志 Pub Date : 2013-08-01
Bing-Ya Guo, Guang-Cheng Xie, Zhao-Jun Duan, Li-Jun Xia
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引用次数: 0

摘要

目的研究重组人 beta-防御素-3(hBD-3)对 BEAS-2B 细胞中白细胞介素-17A(IL-17A)和白细胞介素-22(IL-22)表达的影响:方法:分别用不同浓度的 hBD-3 刺激 BEAS-2B 细胞 6 小时和 24 小时。方法:分别用不同浓度的 hBD-3 刺激 BEAS-2B 细胞 6 小时和 24 小时,用实时 PCR 检测 Toll 样受体 2(TLR2)、IL-17A 和 IL-22 mRNA 的表达水平,用酶联免疫吸附试验检测 IL-17A 和 IL-22 蛋白的表达水平:结果:与 6 小时相比,经 hBD-3 刺激 24 小时后,BEAS-2B 细胞中的 TLR2 mRNA 以浓度和时间依赖性的方式显著增加。在浓度为 100 ng/ml 的刺激下,24 小时后 IL-17A 的 mRNA 和蛋白水平明显升高,但在浓度为 50 ng/ml 的刺激下,6 小时后 IL-17A mRNA 表达升高,蛋白水平无变化。在 50 ng/ml 浓度的 hBD-3 中刺激 6 小时后,IL-22 mRNA 和蛋白表达达到峰值水平,而随着 hBD-3 浓度的增加,IL-22 的 mRNA 和蛋白表达水平下降:重组hBD-3能上调TLR2、IL-17A和IL-22的表达,低浓度的hBD-3主要增加IL-22的表达,而高浓度的hBD-3主要增加IL-17A的表达。这些结果表明,不同浓度的 hBD-3 可能会激活不同的转录因子,而这些转录因子是由 TLR2 介导的,从而启动宿主免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The effects of recombinant human beta-defensin-3 on expression of interleukin-17A and interleukin-22 in BEAS-2B cell].

Objective: To research the effects of recombinant human beta-defensin-3 (hBD-3) on expression of interleukin-17A (IL-17A) and interleukin-22 (IL-22) in BEAS-2B cell.

Methods: The BEAS-2B cells were stimulated with different concentrations of hBD-3 for 6 hours and 24 hours, respectively. Toll-like receptor 2 (TLR2), IL-17A and IL-22 mRNA expression levels were determined by real-time PCR, and the expression levels of IL-17A and IL-22 protein were examined by enzyme linked immune-sorbent assay.

Results: TLR2 mRNA in BEAS-2B cells were significantly increased in a concentration-and time-dependent manner after stimulating by hBD-3 for 24 hours compared to 6 hours. The IL-17A has significantly increased in mRNA and protein levels stimulated 24 hours in a concentration of 100 ng/ml, however, IL-17A mRNA expression has increased while protein didn't change stimulated 6 hours in a concentration of 50 ng/ml. The IL-22 mRNA and protein expression reached peak levels after stimulating in a concentration of 50 ng/ml of hBD-3 while IL-22 expression declined in mRNA and protein levels as the concentration of hBD-3 increased.

Conclusions: Recombinant hBD-3 can up-regulated the expression of TLR2, IL-17A and IL-22, lower concentration of hBD-3 mainly increased the expression of IL-22 while higher concentration of hBD-3 mainly increased the expression of IL-17A. These results show that different concentrations of hBD-3 maybe activate different transcription factors which was mediated by TLR2, initiating host immune response.

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