Long-You Zhao, Yong-Ping Ji, De-Bin Wang, Jie Zhuang, Bin Zhou
{"title":"两种酶联免疫吸附法检测丙型肝炎抗体的比较","authors":"Long-You Zhao, Yong-Ping Ji, De-Bin Wang, Jie Zhuang, Bin Zhou","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.</p><p><strong>Methods: </strong>A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.</p><p><strong>Results: </strong>To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.</p><p><strong>Conclusions: </strong>The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"304-6"},"PeriodicalIF":0.0000,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C].\",\"authors\":\"Long-You Zhao, Yong-Ping Ji, De-Bin Wang, Jie Zhuang, Bin Zhou\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.</p><p><strong>Methods: </strong>A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.</p><p><strong>Results: </strong>To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.</p><p><strong>Conclusions: </strong>The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 4\",\"pages\":\"304-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C].
Objective: To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA.
Methods: A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay (RIBA) and HCV-RNA.
Results: To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40.9%, 59.1% and 2.2% respectively. The positive rates of them were 100%, but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times.
Conclusions: The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.
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