{"title":"云南省分离的肠病毒71型的鉴定与鉴定","authors":"Shi-Wen Zhao, Wei-Dong Yin, Jian-Ping Cun, Qiang Gao, Jie Yin, Guo-Jun Liu, Wen Xu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.</p><p><strong>Methods: </strong>Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.</p><p><strong>Result: </strong>All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.</p><p><strong>Conclusion: </strong>Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"263-5"},"PeriodicalIF":0.0000,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Identification and characterization of enterovirus 71 isolated in Yunnan province].\",\"authors\":\"Shi-Wen Zhao, Wei-Dong Yin, Jian-Ping Cun, Qiang Gao, Jie Yin, Guo-Jun Liu, Wen Xu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.</p><p><strong>Methods: </strong>Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.</p><p><strong>Result: </strong>All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.</p><p><strong>Conclusion: </strong>Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 4\",\"pages\":\"263-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Identification and characterization of enterovirus 71 isolated in Yunnan province].
Objective: Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.
Methods: Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.
Result: All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.
Conclusion: Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
