{"title":"泛醌、beclin-1与肝脏线粒体关系的研究","authors":"Fang Xie, Chao Zhang, Yue-Ke Zhu, Hui Liu, Xue-Mei Liu, Lin Jia, Ke-Fei Wang, De-Xi Chen, Qing-Hua Meng","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To study whether CO-Q10 can protect liver injury caused by acute on chronic liver failure (ACLF) by autophagy.</p><p><strong>Methods: </strong>Rats were separated into three groups: control group, acute on chronic liver failure (ACLF) and intervenient group, liver tissues were observed by optical microscopy and electron microscopy. The levels of Beclin-1 expression were determined by real-time PCR. And Western Blot.</p><p><strong>Results: </strong>Areas of necrosis detected in intervenient group were alleviated than in ACLF significantly. Most mitochondrias had been degradated in ACLF group while alive in intervenient group. Real-time PCR and Western Blot revealed level of beclin-1 in ACLF was lower than control and intervenient group.</p><p><strong>Conclusion: </strong>Intervenient group may ameliorate rat liver injury by promoting autophagy.</p>","PeriodicalId":70973,"journal":{"name":"中华实验和临床病毒学杂志","volume":"27 4","pages":"250-2"},"PeriodicalIF":0.0000,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Research of the relationship of ubiquinone and beclin-1 and liver mitochondria].\",\"authors\":\"Fang Xie, Chao Zhang, Yue-Ke Zhu, Hui Liu, Xue-Mei Liu, Lin Jia, Ke-Fei Wang, De-Xi Chen, Qing-Hua Meng\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To study whether CO-Q10 can protect liver injury caused by acute on chronic liver failure (ACLF) by autophagy.</p><p><strong>Methods: </strong>Rats were separated into three groups: control group, acute on chronic liver failure (ACLF) and intervenient group, liver tissues were observed by optical microscopy and electron microscopy. The levels of Beclin-1 expression were determined by real-time PCR. And Western Blot.</p><p><strong>Results: </strong>Areas of necrosis detected in intervenient group were alleviated than in ACLF significantly. Most mitochondrias had been degradated in ACLF group while alive in intervenient group. Real-time PCR and Western Blot revealed level of beclin-1 in ACLF was lower than control and intervenient group.</p><p><strong>Conclusion: </strong>Intervenient group may ameliorate rat liver injury by promoting autophagy.</p>\",\"PeriodicalId\":70973,\"journal\":{\"name\":\"中华实验和临床病毒学杂志\",\"volume\":\"27 4\",\"pages\":\"250-2\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中华实验和临床病毒学杂志\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验和临床病毒学杂志","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Research of the relationship of ubiquinone and beclin-1 and liver mitochondria].
Objective: To study whether CO-Q10 can protect liver injury caused by acute on chronic liver failure (ACLF) by autophagy.
Methods: Rats were separated into three groups: control group, acute on chronic liver failure (ACLF) and intervenient group, liver tissues were observed by optical microscopy and electron microscopy. The levels of Beclin-1 expression were determined by real-time PCR. And Western Blot.
Results: Areas of necrosis detected in intervenient group were alleviated than in ACLF significantly. Most mitochondrias had been degradated in ACLF group while alive in intervenient group. Real-time PCR and Western Blot revealed level of beclin-1 in ACLF was lower than control and intervenient group.
Conclusion: Intervenient group may ameliorate rat liver injury by promoting autophagy.
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