Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology最新文献_第3页

Repeated passage of freshly isolated group A streptococci on blood agar. II. Effect on adherence capacity. 新鲜分离的A组链球菌在血琼脂上反复传代。2对依从能力的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-12-01 DOI: 10.1111/j.1699-0463.1986.tb03075.x

A Stjernquist-Desatnik, D N Kurl, P Christensen

引用次数: 4

An alternative approach to typing of Streptococcus pneumoniae strains by coagglutination. 一种通过凝血分型肺炎链球菌菌株的替代方法。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-12-01 DOI: 10.1111/j.1699-0463.1986.tb03076.x

L E Smart, J Henrichsen

引用次数: 11

Quantitative determination of beta-lactamase production in Staphylococcus aureus strains compared to qualitative testing by a microbiological clover leaf test, a chromogenic cephalosporin test and a iodometric test. 金黄色葡萄球菌菌株β -内酰胺酶产量的定量测定与微生物三叶草试验、显色头孢菌素试验和碘量试验定性试验的比较

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-12-01 DOI: 10.1111/j.1699-0463.1986.tb03077.x

J O Jarløv, V T Rosdahl

{"title":"Quantitative determination of beta-lactamase production in Staphylococcus aureus strains compared to qualitative testing by a microbiological clover leaf test, a chromogenic cephalosporin test and a iodometric test.","authors":"J O Jarløv, V T Rosdahl","doi":"10.1111/j.1699-0463.1986.tb03077.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03077.x","url":null,"abstract":"One hundred and twelve beta-lactamase producing strains of Staphylococcus aureus with known quantitative production were investigated together with 45 non-beta-lactamase producing derivatives by three qualitative beta-lactamase tests: the microbiological clover leaf test, the iodometric tube test and a chromogenic cephalosporin disc test. For the beta-lactamase producing strains strong positive reactions in the qualitative tests were not correlated with high beta-lactamase production, large degree of extracellularity or high induction ratio in any of the tests. Low beta-lactamase production was not correlated to weak reactions. Best results in identifying beta-lactamase production were obtained using the iodometric test in which 109 of 112 beta-lactamase producing strains were positive and no false positive reactions were observed. Some strains had only weak positive reactions but they were easily read. The clover leaf test and the chromogenic cephalosporin test detected 105 and 107 of 112 strains, respectively. The chromogenic cephalosporin disc test had more weakly reacting strains, which were difficult to read, especially among strains of phage type 95. Three non-beta-lactamase producing strains of phage type 95 were positive in the clover leaf test. It is concluded that the iodometric tube test is the most useful one for qualitative determination of beta-lactamase production in S. aureus. It is recommended as control when normal susceptibility testing for penicillin has shown susceptibility or doubtful susceptibility. Furthermore, it is useful when no susceptibility testing has been carried out and a rapid result is needed.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"415-21"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03077.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14618729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Non-immune F(ab')2- and Fc-mediated interactions of mammalian immunoglobulins with S. aureus and group C and G streptococci. 哺乳动物免疫球蛋白与金黄色葡萄球菌、C群和G群链球菌的非免疫F(ab’)2-和fc介导的相互作用

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-12-01 DOI: 10.1111/j.1699-0463.1986.tb03071.x

M Erntell, E B Myhre, G Kronvall

{"title":"Non-immune F(ab')2- and Fc-mediated interactions of mammalian immunoglobulins with S. aureus and group C and G streptococci.","authors":"M Erntell, E B Myhre, G Kronvall","doi":"10.1111/j.1699-0463.1986.tb03071.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03071.x","url":null,"abstract":"The distribution among mammalian species of non-immune F(ab')2- and Fc-mediated immunoglobulin interactions with surface proteins of S. aureus (protein A) and of group C and G streptococci was studied. Serum samples from 48 mammalian species representing 15 orders were first tested for their capacity to inhibit streptococcal F(ab')2-mediated binding; 26 of these sera were also tested for streptococcal IgG Fc-mediated binding. Analogous inhibition experiments were then carried out with staphylococci. All mammalian species studied inhibited both types of immunoglobulin binding to streptococci, viz the serum samples contained both F(ab')2- and Fc-reactive immunoglobulins. The reactivity was equal to that of human serum in 26 out of 47 mammalian sera. Seven sera showed a low degree of inhibition compared to human serum. The inhibiting capacities of the two streptococcal non-immune interactions showed a direct correlation (r = 0.91, p less than 0.0001 for the r-value) for individual species. The inhibition patterns observed with S. aureus differed from the profiles recorded with the streptococcal strains, suggesting that these organisms interact with separate sites on the immunoglobulin molecules. Isolated F(ab')2-binding was recorded in 5 out of 24 sera, and Fc-binding alone was noted in 7 sera. Taken together, the present studies demonstrate that mammalian immunoglobulins possess F(ab')2- and Fc-binding sites for protein A and for receptors on group C and G streptococci. The F(ab')2-mediated binding to streptococci is associated with Fc-reactivity, in contrast to protein A which may interact exclusively with a complementary structure in either the F(ab')2- or the Fc-portion of the immunoglobulins.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"377-85"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03071.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14686892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

A thin-layer chromatographic method for separating methyl esters of mycobacterial mycolic acids. 分离分枝杆菌霉菌酸甲酯的薄层色谱方法。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-12-01 DOI: 10.1111/j.1699-0463.1986.tb03070.x

P Valero-Guillén, F Martin-Luengo, J Jimenez, L Larsson

引用次数: 4

Effect of encephalomyocarditis (EMC) virus on murine foetal and placental growth monitored by quantification of maternal plasma levels of pregnancy-associated murine protein-2 and alpha-fetoprotein. 通过测定母体血浆妊娠相关蛋白-2和甲胎蛋白水平监测脑心肌炎(EMC)病毒对小鼠胎儿和胎盘生长的影响

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-10-01 DOI: 10.1111/j.1699-0463.1986.tb03063.x

J Hau, K Buschard

引用次数: 7

Independent binding of native and aggregated IgG in group A streptococci. A组链球菌中天然和聚集IgG的独立结合。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-10-01 DOI: 10.1111/j.1699-0463.1986.tb03062.x

C Schalén, D Kurl, P Christensen

{"title":"Independent binding of native and aggregated IgG in group A streptococci.","authors":"C Schalén, D Kurl, P Christensen","doi":"10.1111/j.1699-0463.1986.tb03062.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03062.x","url":null,"abstract":"Irrespective of IgG Fc-receptor activity, earlier characterized, many group A streptococci were recently found to bind aggregated IgG Fab and/or light chains. In the present study, binding of glutaraldehyde-aggregated, radiolabelled, intact human IgG (a*IgG) to group A streptococci was tested, and strains representing several M-types were found reactive. In particular, high binding was observed among type M12 strains, earlier found devoid of Fc-receptors for monomeric IgG; accordingly, unlabelled, native IgG had little influence on the binding. The sites binding a*IgG were highly sensitive to trypsin and relatively resistant to heat treatment. The binding to M12 was inhibited by human fibrinogen and, to a lesser extent, by heat-aggregated serum albumin. Rabbit antiserum to M12 was more inhibitory than antiserum to a heterologous type of group A streptococci or normal rabbit serum. Our results indicate that streptococcal M-protein binds a*IgG by a multipoint requiring interaction of low specificity and that previously described Fc-receptors binding native IgG are not involved. For comparison, in Cowan I staphylococci and one strain of group G streptococci tested, high binding of a*IgG was also observed; however, this binding was inhibited by native IgG, indicating that protein A and group G streptococcal Fc-receptor, earlier known to bind untreated IgG, also bound a*IgG.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 5","pages":"333-8"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03062.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14661093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Population analysis of susceptibility to ciprofloxacin and nalidixic acid in Staphylococcus, Pseudomonas aeruginosa, and Enterobacteriaceae. 葡萄球菌、铜绿假单胞菌和肠杆菌科细菌对环丙沙星和萘啶酸的敏感性群体分析。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-10-01 DOI: 10.1111/j.1699-0463.1986.tb03066.x

P Søgaard, B Gahrn-Hansen

{"title":"Population analysis of susceptibility to ciprofloxacin and nalidixic acid in Staphylococcus, Pseudomonas aeruginosa, and Enterobacteriaceae.","authors":"P Søgaard, B Gahrn-Hansen","doi":"10.1111/j.1699-0463.1986.tb03066.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03066.x","url":null,"abstract":"Susceptibility to ciprofloxacin (Cfl) and nalidixic acid (Nal) was tested in vitro by means of the population analysis technique against six strains of Staphylococcus, one strain of Pseudomonas aeruginosa, and seven strains from five genera of Enterobacteriaceae. All strains of Staphylococcus were uniformly resistant to Nal as was the Pseudomonas aeruginosa strain, all bacteria being resistant to 250- less than 500 micrograms/ml. The Enterobacteriaceae were heterogeneous as regards susceptibility to Nal. With some strains minority populations of highly-resistant bacteria could be detected with frequencies of about 10(-6.3). The MIC for Cfl for the staphylococci varied between 0.25 and 0.50 microgram/ml. There were no differences in MIC of penicillinase-producing and penicillin-susceptible strains, either in Staphylococcus aureus or in Staphylococcus epidermidis. The MIC for Cfl in the enterobacteria varied between 0.004 and 0.03 microgram/ml. The MIC for Cfl in the Pseudomonas aeruginosa was 0.25 microgram/ml. MIC for Cfl increased in all strains when the parental strains were compared to bacteria selected from the plates with the highest concentration permitting growth, indicating heterogeneity against Cfl. But while the MIC of the selected enterobacteria were lower than one fourth of the level obtainable in serum, the MIC of the selected staphylococci and Pseudomonas aeruginosa were either exceeding the level obtainable in serum or were only a little lower than this level. While Cfl thus seems to be a promising antimicrobial agent in the treatment of infections caused by enterobacteria, the suitability for infections caused by staphylococci and Pseudomonas aeruginosa should be further explored.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 5","pages":"351-6"},"PeriodicalIF":0.0,"publicationDate":"1986-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03066.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14229441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Collection of bacterial aerosols by means of slit-sampler: a face-mask study. 用狭缝取样器收集细菌气溶胶:面罩研究。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-10-01 DOI: 10.1111/j.1699-0463.1986.tb03060.x

K L Melvaer, H Ringstad

引用次数: 0

The ultrastructure of antibiotic-susceptible and multi-resistant strains of group JK diphtheroid rods isolated from clinical specimens. 临床分离的JK组白喉棒耐药和多耐药菌株的超微结构。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1986-10-01 DOI: 10.1111/j.1699-0463.1986.tb03057.x

J Blom, O Heltberg

引用次数: 3