Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology最新文献_第10页

Herpes simplex virus type-specific antibodies detected by indirect and competition ELISA. Comparison of sera from patients with carcinoma of the uterine cervix, age matched controls and patients with recurrent genital herpes. 间接和竞争ELISA法检测单纯疱疹病毒类型特异性抗体。宫颈癌患者、年龄匹配对照组及复发性生殖器疱疹患者血清的比较。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-06-01

S N Najem, B F Vestergaard, C W Potter

引用次数: 0

Genetic transformation in Streptococcus sanguis. Kinetics of production in different media and specific interaction of competence factor and competence factor inactivator. 血链球菌的遗传转化。能力因子与能力因子失活剂在不同介质中的生产动力学及特定的相互作用。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-06-01

P Gaustad

{"title":"Genetic transformation in Streptococcus sanguis. Kinetics of production in different media and specific interaction of competence factor and competence factor inactivator.","authors":"P Gaustad","doi":"","DOIUrl":"","url":null,"abstract":"The production and the persistence of competence factor (CF) and competence factor inactivator (CFI) of four different strains of Streptococcus sanguis in three media (Todd Hewitt broth, medium II and medium IV) have been examined with respect to kinetics. Both the medium used and the strains examined offered implications on the activities of CF and CFI. CF was not detectable in the culture filtrates of strains Wicky (NCTC 9124) and 445. Strain Blackburn (NCTC 10231) showed activity only in Todd Hewitt broth and strain 14567 in Todd Hewitt broth and medium IV. The activity persisted for 2 to 7 hours during exponential growth. In culture filtrates of the strains Wicky and Blackburn the three media yielded high CFI activity persisting during the entire growth period. The strains 445 and 14567 were less potent CFI producers in medium II and medium IV, and no CFI was detected in the culture filtrates from Todd Hewitt broth. Medium II was the most suitable medium for CFI production and seems appropriate for further studies and isolation of the factor. In medium IV no strain had CF activity. This medium was supplemented with serum, albumin, tyrosine or glutamic acid, and CF and CFI production and persistence by strain 14567 were studied in relation to growth phases. CF and CFI activities were observed in the different supplemented media during short periods of growth, but could not be related to any given phase of growth. The presence of CFI in a culture eliminated or reduced the CF activity, and a specific interaction of CF and CFI was observed. The CFI from the strain Wicky inactivated CF from the strains Challis (NCTC 7868) and Blackburn, but not from the strains 13b and 14567. The CFI from the strains 445 and 14567 reduced the activity of CF produced by the strains 13b and 14567, but not CF from the strains Challis and Blackburn.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"91 3","pages":"193-200"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17929880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-antigenic corss-reactivity in Fusobacterium nucleatum: chemotype V lipopolysaccharides. 核梭杆菌o抗原交叉反应性:化学型V脂多糖。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-04-01

B Adnegard, T Horstad

引用次数: 0

The genetic control of virulence in group A streptococci. I. Conjugal transfer of plasmids and their effect on expression of some host cell properties. A群链球菌毒力的遗传控制。质粒的共轭转移及其对宿主细胞某些特性表达的影响。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-02-01

L E Ravdonikas

引用次数: 0

A rapid method to produce a sensitive Limulus amoebocyte lysate (LAL). I. Evaluation of inter and intra batch differences in LAL and hemolymph from Limulus polyphemus. 一种快速制备敏感鲎试剂的方法。1 .水蛭LAL和血淋巴的批间和批内差异评价。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-02-01

M Tvede, L Baek

{"title":"A rapid method to produce a sensitive Limulus amoebocyte lysate (LAL). I. Evaluation of inter and intra batch differences in LAL and hemolymph from Limulus polyphemus.","authors":"M Tvede, L Baek","doi":"","DOIUrl":"","url":null,"abstract":"An improved method for preparing Limulus amoebocyte lysate (LAL) is described and compared with two other well-known procedures. In five Limuli, three different bleeding procedures and three different cell rupture methods were studied. Increased sensitivity of LAL was accomplished by avoidance of the anticoagulant N-ethylmaleimid (NEM) in the bleeding procedure, optimal methods for mechanical cell rupture and use of pyrogen free conditions. Reaction of LAL with as little as 10(-15) gram Lipopolysaccharide (LPS) per ml was generally obtained. To evaluate a possible explanation for the differences in reactivity of LAL between different Limuli, found in one experiment, six Limuli were bled, and the pH and the concentration of protein, Ca2+, Mg2+, Cu2+, Na+ and K+ were determined in LAL and cell-free hemolymph. Inter and intra batch variations were found in LAL, but there was no correlation between the sensitivity of LAL and the content of the above mentioned constituents in LAL and cell-free hemolymph. Experiments with use of NEM in the bleeding procedure and addition of NEM to homogenized cells in different concentrations, showed that NEM inhibits the reactivity of LAL to LPS. It is concluded that the modified method of producing LAL by bleeding without NEM and by using optimal methods for mechanical cell rupture is quick, simple and produces a very sensitive reagent for the Limulus test.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"91 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17920144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance types in Escherichia coli. III. beta-lactamase production. 大肠杆菌的耐药类型。3beta-lactamase生产。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-02-01

P Søgaard

{"title":"Resistance types in Escherichia coli. III. beta-lactamase production.","authors":"P Søgaard","doi":"","DOIUrl":"","url":null,"abstract":"The beta-lactamase activity with ampicillin (A) and cephalothin (Ce) as substrate was examined in 22 E. coli strains by a micro-iodometric - (MIA) and an ultraviolet assay (UVA). The strains were divided into three groups according to their sensitivity to A and carbenicillin (Ca). Group 1 contained 6 A-Ca sensitive (A-s/Ca-s) strains, group 2 contained 8 A-resistant (A-r)/Ca-s strains and group 3 eight A-r/Ca-r ones. MIA with A as substrate showed high activity in group 3. The activities in groups 1 and 2 did not differ and were very low. Group 1 had the lowest and group 3 the highest IC50 with A. UVA with Ce as substrate showed high activity in group 2, lower in group 3 and lowest in group 1. IC50 corresponded to this order. MIA with Ce as substrate showed high activity (measured 25 min after enzyme was added) in group 3, lower activity in group 2 and lowest in group 1. The initial velocity was highest in group 2, but decreased with time. In group 3 the velocity increased with time. Group 2 (A-r/Ca-s) strains contained cephalothinases that apparently were inhibited by iodine. The beta-lactam resistance was probably chromosomally mediated. Group 3 (A-r/Ca-r) strains contained beta-lactamases with predominantly ampicillinase activity and were not inhibited by iodine. The A-resistance was probably plasmid mediated.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"91 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17407027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genetic control of virulence in group A streptococci. II. Trigger effect by plasmids on anti-phagocytic activity, opacity factor and IgG and IgA Fc-receptors. A群链球菌毒力的遗传控制。2质粒对抗吞噬活性、不透明因子及IgG和IgA fc受体的触发作用。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-02-01

L A Burova, L E Ravdonikas, P Christensen, C Schalén, A A Totolian

{"title":"The genetic control of virulence in group A streptococci. II. Trigger effect by plasmids on anti-phagocytic activity, opacity factor and IgG and IgA Fc-receptors.","authors":"L A Burova, L E Ravdonikas, P Christensen, C Schalén, A A Totolian","doi":"","DOIUrl":"","url":null,"abstract":"The erythromycin-resistance marker of two plasmids, pSM19035 and pERL1, could be transferred by conjugation in matings within group A streptococci and between group H and group A streptococci, with a frequency of 10(-5) to 8 X 10(-7). Two of the recipient strains used, 22v-1 and 22v-2, showed traces of opacity factor (OF) activity. This activity increased markedly upon transfer of both plasmids to the strains, whereas a number of other recipients, viz. 22h-21, 12Teiko-1 and Challis 6-1, remained OF-negative after conjugation. Furthermore, the transconjugants resulting from matings using strains 22v-1 and 22v-2 as recipients expressed anti-phagocytic activity. Attempts were made to type the transconjugants but they did not belong to type M12, M22 (the types from which the parents were derived) M3 or M1. Concomitant with the expression of anti-phagocytic activity, IgG and IgA Fc-receptor activity occurred in the transconjugants of 22v-1 and 22v-2, whereas the donor and recipient cells were without receptors. It was not possible to demonstrate extrachromosomal DNA in transconjugants possessing anti-phagocytic, OF or Fc-receptor activity, although they retained their ability to serve as donors of the Emr marker. It is suggested that the triggering by plasmids of anti-phagocytic activity, OF and IgG and IgA Fc-receptors is due to insertion of plasmid DNA into the chromosome.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"91 1","pages":"61-7"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17407029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of frozen erythrocytes in macrophage studies. 2. Attachment and phagocytosis mediated by the two immunological receptors of the macrophages. 巨噬细胞研究中冷冻红细胞的使用。2. 巨噬细胞的两种免疫受体介导的附着和吞噬作用。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1983-02-01

V Myhrvold, J Jonsen, B Mørland

引用次数: 0

Bacterial adhesiveness and invasiveness in cell culture monolayer. 2. In vitro invasiveness of 45 strains belonging to the family Enterobacteriaceae. 细菌在细胞培养单层中的粘附性和侵袭性。2. 45株肠杆菌科细菌的体外侵袭性研究。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1982-12-01

G Bukholm, J Lassen

引用次数: 0

Experimental endocarditis in rabbits. 7. Results of long-term combined therapy of Streptococcus faecalis endocarditis with penicillin and gentamicin. 兔实验性心内膜炎。7. 青霉素与庆大霉素长期联合治疗粪链球菌心内膜炎的疗效观察。

Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology Pub Date : 1982-08-01

E Gutschik

{"title":"Experimental endocarditis in rabbits. 7. Results of long-term combined therapy of Streptococcus faecalis endocarditis with penicillin and gentamicin.","authors":"E Gutschik","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to investigate the efficacy of long-term penicillin-gentamicin treatment on experimental S. faecalis endocarditis due to selected strains; one strain was homogeneously resistant to 8000 micrograms/ml streptomycin (IC50 and MIC greater than 8000 micrograms ml), the other strain heterogeneously resistant to 8000 micrograms/ml streptomycin (IC50: 3300 micrograms/ml, MIC: greater than 8000 micrograms/ml. Both strains showed low-grade resistance to gentamicin (MIC 10.5 and 25 micrograms/ml, respectively). The results showed that there was significant effect of the treatment in rabbits with endocarditis due to both strains, as measured by lowered mortality and by high bacteriologic cure rate. Despite effective antibiotic treatment, a high incidence (57%) of congestive heart failure was noted in rabbits late in the treatment period or after termination of antibiotic treatment, probably due to healing processes on the aortic valves. In human cases of S. faecalis endocarditis the antibiotic treatment should be either penicillin plus streptomycin or penicillin plus gentamicin. However, in cases where the infecting strain is homogeneously resistant to 8000 micrograms/ml streptomycin, the treatment with penicillin plus gentamicin is clearly the drug combination of choice.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"90 4","pages":"295-302"},"PeriodicalIF":0.0,"publicationDate":"1982-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17864665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0