{"title":"Serology of Campylobacter fetus ss. jejuni. 2 Serotyping of live bacteria by slide, latex and co-agglutination tests.","authors":"T. Kosunen, D. Danielsson, J. Kjellander","doi":"10.1111/J.1699-0463.1982.TB00104.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1982.TB00104.X","url":null,"abstract":"Antisera against 12 campylobacter strains (8 C. fetus ss. jejuni, 2 ss fetus and 2 ss. intestinalis) were obtained from rabbits immunized with formalin treated bacteria. Cross testing of live boiled and autoclaved organisms of these strains were performed with direct slide and tube agglutinations, and with agglutinations of latex particles and protein A-containing staphylococci coated with antibodies. Confirmed by absorption experiments both heat labile and heat stable antigens were demonstrated. The highest specificity for serological differentiation was obtained with slide and latex agglutination with live bacteria, which were subsequently used for differentiation of C. fetus ss. jejuni strains. For this purpose we applied 22 C. fetus ss. jejuni antisera absorbed with boiled homologous bacteria. Clearcut homologous reactions, with few and weak crossreactions, indicated the presence of a variety of heat labile antigens, which could form a basis for serotyping. Preliminary typing of 170 clinical isolates with slide agglutination using 17 unabsorbed C. fetus ss. jejuni antisera demonstrated the applicability of this approach for epidemiological studies. We found antigenically similar strains in family outbreaks, antigenic differences in strains of different origin, new combinations of cross reactions not seen in strains used in immunization, antigenically \"new\" strains not reacting with these antisera, and the dominance of a few antigenic types among the isolates.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"29 1","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90010220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Herpes simplex virus type-specific antibodies detected by indirect and competition ELISA. Comparison of sera from patients with carcinoma of the uterine cervix, age matched controls and patients with recurrent genital herpes.","authors":"S. Najem, B. Vestergaard, C. Potter","doi":"10.1111/J.1699-0463.1983.TB00034.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1983.TB00034.X","url":null,"abstract":"A total of 85 human sera (32 from patients with carcinoma of the uterine cervix, 32 from matched controls, and 21 from patients with recurrent genital herpes) were tested in two different ELISAs for detection of HSV type-specific antibodies. The prevalence of HSV type 2 specific antibodies in the three groups were 69, 37 and 71% respectively. The prevalence of HSV type 1 specific antibodies in the three groups were 88, 75 and 76% respectively. Analysis of IgG and total antibodies gave identical results.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"42 1","pages":"205-7"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85094326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Burova, L. E. Ravdonikas, P. Christensen, C. Schalén, A. Totolian
{"title":"The genetic control of virulence in group A streptococci. II. Trigger effect by plasmids on anti-phagocytic activity, opacity factor and IgG and IgA Fc-receptors.","authors":"L. Burova, L. E. Ravdonikas, P. Christensen, C. Schalén, A. Totolian","doi":"10.1111/J.1699-0463.1983.TB00010.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1983.TB00010.X","url":null,"abstract":"The erythromycin-resistance marker of two plasmids, pSM19035 and pERL1, could be transferred by conjugation in matings within group A streptococci and between group H and group A streptococci, with a frequency of 10(-5) to 8 X 10(-7). Two of the recipient strains used, 22v-1 and 22v-2, showed traces of opacity factor (OF) activity. This activity increased markedly upon transfer of both plasmids to the strains, whereas a number of other recipients, viz. 22h-21, 12Teiko-1 and Challis 6-1, remained OF-negative after conjugation. Furthermore, the transconjugants resulting from matings using strains 22v-1 and 22v-2 as recipients expressed anti-phagocytic activity. Attempts were made to type the transconjugants but they did not belong to type M12, M22 (the types from which the parents were derived) M3 or M1. Concomitant with the expression of anti-phagocytic activity, IgG and IgA Fc-receptor activity occurred in the transconjugants of 22v-1 and 22v-2, whereas the donor and recipient cells were without receptors. It was not possible to demonstrate extrachromosomal DNA in transconjugants possessing anti-phagocytic, OF or Fc-receptor activity, although they retained their ability to serve as donors of the Emr marker. It is suggested that the triggering by plasmids of anti-phagocytic activity, OF and IgG and IgA Fc-receptors is due to insertion of plasmid DNA into the chromosome.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"53 1","pages":"61-7"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75550306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The antibiotic effect of the anti-depressive drug femoxetine and its stereo-isomeric analogs on diarrhoea producing enterobacteriaceae.","authors":"J. Kristiansen, I. Mortensen, K. Gaarslev","doi":"10.1111/J.1699-0463.1986.TB03027.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1986.TB03027.X","url":null,"abstract":"The present investigation has been undertaken to illustrate the antibacterial effect on 20 diarrhoea producing enterobacteriaceae of an anti-depressive drug available as femoxetine and its three analogs. It has been shown that the stereo-isomeric trans forms of femoxetine are more than twice as active as the cis forms and inhibited all the strains below 400 microgram/ml (1.2 mM). The two cis compounds only inhibited 11 and 9 of the 20 strains respectively in the investigated area 100 microgram/ml - 800 microgram/ml (0.3 mM - 2.4 mM). Our investigations point out that the bacterial cell has a target for psychopharmacologically active agents. Thus the known psychopharmaca and their stereo-isomeric analogs may represent a pool of potentially new antimicrobial drugs. Furthermore the bacterial model may be useful as a model system in the study of the interaction of neuropharmacological agents and other membrane active compounds with biological membranes.","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"30 1","pages":"103-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82251452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Infection due to \"Corynebacterium ulcerans\", producing diphtheria toxin--a case report from Denmark.","authors":"C Pers","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A diphtheria toxin producing strain of \"C. ulcerans\" was isolated from the throat of a 9-year-old boy who was previously immunized against diphtheria. This is the first reported case of human infection due to this organism in Denmark.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"95 6","pages":"361-2"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14564029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cutaneous alternariosis. Case report.","authors":"J Stenderup, M Bruhn, C Gadeberg, A Stenderup","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A case of cutaneous alternariosis is reported in the wife of a 73-year-old farmer. The diagnosis was verified culturally and histologically. The skin lesions were localized to legs, arms and face and were characterized by alternating spontaneous healing and formation of new lesions possibly due to autoinoculation from the lesions or re-exposure to the environment. The patient had a decreased resistance to infections owing to cancer - for which she had received X-ray treatment, chemotherapy and cortisone. This is the first reported Danish case.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"95 1","pages":"79-81"},"PeriodicalIF":0.0,"publicationDate":"1987-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14686803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The antibacterial effect of selected phenothiazines and thioxanthenes on slow-growing mycobacteria.","authors":"J E Kristiansen, B Vergmann","doi":"10.1111/j.1699-0463.1986.tb03073.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03073.x","url":null,"abstract":"<p><p>The aim of the present investigation was to illustrate the antibacterial effect of various phenothiazine and thioxanthene derivatives on mycobacteria in vitro. It was demonstrated that clopenthixol is about twice as potent as chlorpromazine (CPZ) and levomepromazine-maleate is about half as potent as CPZ, measured by the inhibitory effect on the growth of the mycobacterial strains. Measured in the same way the stereoisomeric compounds of flupenthixol are shown to be more potent than the stereo-isomeric compounds of clopenthixol and chlorprothixen. The two last-named compounds are equal in potency. The stereo-isomeric analogs of the thioxanthene derivatives are equal in antibacterial potency against the slow-growing mycobacteria. The mycobacterial strains investigated show no difference in sensitivity between the cis (Z)--and and trans (E)--compounds of the thioxanthenes. It seems particularly promising that also the more resistant mycobacteria other than Mycobacterium tuberculosis, e.g. M. avium and M. intracellulare, are sensitive in the concentration range investigated. Considered as a whole, these results might be a stimulus to investigate the antimicrobial effect of the thioxanthenes in vivo.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"393-8"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03073.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14236407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"beta-lactamase production in coagulase-negative micrococcaceae.","authors":"V T Rosdahl, J O Jarløv, A M Knudsen","doi":"10.1111/j.1699-0463.1986.tb03078.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03078.x","url":null,"abstract":"<p><p>A total of 8 penicillin-susceptible and 55 penicillin-resistant coagulase-negative Micrococcaceae were investigated for their beta-lactamase production by one quantitative and three qualitative methods. Seven of the 8 penicillin-susceptible strains had no beta-lactamase production; one had a very low production. Among the 55 penicillin-resistant strains, 54 produced beta-lactamase (mean 63 units/mg bacteria). Methicillin/gentamicin-resistant strains produced less enzyme than strains susceptible to these antibiotics. The percentage extracellularity (mean 18%) was lower than in Staphylococcus aureus, whereas the induction ratio (mean 56) was higher. No significant difference between the different species could be observed, but 70% of the strains were Staphylococcus epidermidis. Among the 55 beta-lactamase producing strains, the microbiological clover-leaf method detected 51, the iodometric method 40 and the chromogenic cephalosporin method 38. None of the three qualitative methods gave any false positive reactions.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"423-7"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03078.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14673629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evidence that an extract of Staphylococcus capitis can precipitate human serum HDL2.","authors":"A Osland, A T Høstmark","doi":"10.1111/j.1699-0463.1986.tb03072.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03072.x","url":null,"abstract":"<p><p>The high density lipoprotein (HDL) fraction involved in the precipitation with Staphylococcus capitis can be quantitated by single radial diffusion in agar containing an extract of the bacteria. Both whole serum and purified HDL produce a precipitation ring with bacterial extract. When dilutions of serum are tested, the squared radius of the precipitation ring plotted against serum concentration gives a linear relationship. Cumulative density flotation of HDL, followed by quantitative analysis of precipitating activity and apo A, indicates that the precipitating activity is present in the less dense fraction of serum HDL (i.e. HDL2). This suggestion is supported by the finding that the precipitating activity correlates positively with HDL2 (but not with HDL3) in human sera. Precipitating activity was higher in sera from women than from men, in accordance with the sex difference in serum HDL. The results raise the question whether precipitation with an extract of S. capitis might serve as a simple, direct method to assess serum HDL2 levels.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"387-92"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03072.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14686893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vaginal lactobacilli inhibiting growth of Gardnerella vaginalis, Mobiluncus and other bacterial species cultured from vaginal content of women with bacterial vaginosis.","authors":"A Skarin, J Sylwan","doi":"10.1111/j.1699-0463.1986.tb03074.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1986.tb03074.x","url":null,"abstract":"<p><p>On a solid agar medium the growth-inhibitory effect of 9 Lactobacillus strains cultured from vaginal content was tested on bacteria cultured from vaginal content of women with bacterial vaginosis: Mobiluncus, Gardnerella vaginalis, Bacteroides and anaerobic cocci. Inhibition zones were observed in the growth of all of the strains isolated from women with bacterial vaginosis around all lactobacilli. The inhibitory effect of the lactobacilli was further tested on various anaerobic and facultatively anaerobic species, both type strains and fresh extragenitally cultured strains. Four Bacteroides fragilis strains as well as 2 out of 4 Staphylococcus aureus strains were clearly inhibited by the lactobacilli. The inhibition zones were generally wider at pH 5.5 than at 6.0. For all inhibited strains, (the S. aureus excepted) a low pH on the agar around the lactobacilli correlated to wider growth-inhibition zones.</p>","PeriodicalId":7045,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology","volume":"94 6","pages":"399-403"},"PeriodicalIF":0.0,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1699-0463.1986.tb03074.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14618728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}