Acta neurobiologiae experimentalis最新文献_第5页

Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model 左乙拉西坦对颞叶癫痫模型中 DNA 氧化和谷胱甘肽含量的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-19 DOI: 10.55782/ane-2024-2537

Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez

{"title":"Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model","authors":"Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez","doi":"10.55782/ane-2024-2537","DOIUrl":"https://doi.org/10.55782/ane-2024-2537","url":null,"abstract":"Levetiracetam (LEV) is a drug commonly used as an anticonvulsant. However, recent evidence points to a possible role as an antioxidant. We previously demonstrated the antioxidant properties of LEV by significantly increasing catalase and superoxide dismutase activities and decreasing the hydrogen peroxide (H2O2) levels in the hippocampus of rats with temporal lobe epilepsy (TLE) showing scavenging properties against the hydroxyl radical. The aim of the present work was to evaluate, the effect of LEV on DNA oxidation, by determining 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG) levels, and glutathione content, through reduced (GSH) and oxidized (GSSG) glutathione levels, in the hippocampus of rats with TLE. Male Wistar rats were assigned to the control (CTRL), CTRL+LEV, epileptic (EPI) and EPI+LEV groups. TLE was induced using the lithium‑pilocarpine model. Thirteen weeks after TLE induction, LEV was administered for one week through osmotic pumps implanted subcutaneously. The determination of 8‑OHdG, GSH and GSSG levels were measured using spectrophotometric methods. We showed that LEV alone significantly increased 8‑OHdG and GSSG levels in the hippocampus of control rats compared to those in epileptic condition. No significant differences in GSH levels were observed. LEV could induce changes in the hippocampus increasing DNA oxidation and GSSG levels under nonepileptic condition but not protecting against the mitochondrial dysfunction observed in TLE probably by mechanisms related to changes in chromatin structure, neuroinflammation and alterations in redox components.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 2","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse 将本征信号光学成像作为描述幼鼠初级视觉皮层定向敏感性的工具

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-29 DOI: 10.55782/ane-2024-2397

I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel

{"title":"Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse","authors":"I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel","doi":"10.55782/ane-2024-2397","DOIUrl":"https://doi.org/10.55782/ane-2024-2397","url":null,"abstract":"We employed intrinsic signal optical imaging (ISOI) to investigate orientation sensitivity bias in the visual cortex of young mice. Optical signals were recorded in response to the moving light gratings stimulating ipsi‑, contra‑ and binocular eye inputs. ISOI allowed visualization of cortical areas activated by gratings of specific orientation and temporal changes of light scatter during visual stimulation. These results confirmed ISOI as a reliable technique for imaging the activity of large populations of neurons in the mouse visual cortex. Our results revealed that the contralateral ocular input activated a larger area of the primary visual cortex than the ipsilateral input, and caused the highest response amplitudes of light scatter signals to all ocular inputs. Horizontal gratings moved in vertical orientation induced the most significant changes in light scatter when presented contralaterally and binocularly, surpassing stimulations by vertical or oblique gratings. These observations suggest dedicated integration mechanisms for the combined inputs from both eyes. We also explored the relationship between point luminance change (PLC) of grating stimuli and ISOI time courses under various orientations of movements of the gratings and ocular inputs, finding higher cross-correlation values for cardinal orientations and ipsilateral inputs. These findings suggested specific activation of different neuronal assemblies within the mouse’s primary visual cortex by grating stimuli of the corresponding orientation. However, further investigations are needed to examine this summation hypothesis. Our study highlights the potential of optical imaging as a valuable tool for exploring functional‑anatomical relationships in the mouse visual system.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"15 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alarin potentiates ongoing epileptiform activity in rat brain slices: an in vitro electrophysiological study 阿拉林能增强大鼠大脑切片中的持续癫痫样活动：体外电生理学研究

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-21 DOI: 10.55782/ane-2024-2520

Ömer Faruk Kalkan, Hilal Öztürk, Zafer Şahin, Harun Başoğlu, Selcen Aydin Abidin, İ. Abidin

{"title":"Alarin potentiates ongoing epileptiform activity in rat brain slices: an in vitro electrophysiological study","authors":"Ömer Faruk Kalkan, Hilal Öztürk, Zafer Şahin, Harun Başoğlu, Selcen Aydin Abidin, İ. Abidin","doi":"10.55782/ane-2024-2520","DOIUrl":"https://doi.org/10.55782/ane-2024-2520","url":null,"abstract":"Alarin is a newly discovered neuropeptide that belongs to the galanin peptide family with a wide range of bioactivity in the nervous system. Its function in the brain’s autonomic areas has been studied, and it has been reported that alarin is involved in the regulation of excitability in hypothalamic neurons. Its role in the regulation of excitability in the hippocampus, however, is unknown. In this study, we investigated if alarin induced any synchronous discharges or epileptiform activity, and if it had any effect on already initiated epileptiform discharges. We used thick acute horizontal hippocampal slices obtained from 30‑ to 35‑day‑old rats. Extracellular field potential recordings were evaluated in the CA1 region of the hippocampus. Our data demonstrated that, alarin application did not result in any epileptiform activity or abnormal discharges. 4‑aminopyridine was applied to induce epileptiform activity in the slices. We found that alarin increased the frequency of interictal‑like events and the mean power of local field potentials in the CA1 region of the hippocampus, which was induced by 4‑aminopyridine. These results demonstrated for the first time that alarin has a modulatory effect on synchronized neuronal discharges and showed the contribution of the neuropeptide alarin to epilepsy‑like conditions.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"20 6","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140444422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abnormal expression of long non‑coding RNA LINC01270 in glioma and its correlation with X‑ray computed tomography signs 胶质瘤中长非编码 RNA LINC01270 的异常表达及其与 X 射线计算机断层扫描征象的相关性

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-21 DOI: 10.55782/ane-2024-2521

Ying Liu, Yuntao Zhang, Yongjun E, Xianglin Zhang, Heji Ma, Furen Dong

{"title":"Abnormal expression of long non‑coding RNA LINC01270 in glioma and its correlation with X‑ray computed tomography signs","authors":"Ying Liu, Yuntao Zhang, Yongjun E, Xianglin Zhang, Heji Ma, Furen Dong","doi":"10.55782/ane-2024-2521","DOIUrl":"https://doi.org/10.55782/ane-2024-2521","url":null,"abstract":"This study focused on the association of LINC01270 and computed tomography (CT) signs with glioma development, to evaluate their potential in the early detection of glioma. Serum LINC01270 was evaluated in glioma patients and healthy individuals using PCR. The involvement of LINC01270 in glioma onset and development was evaluated by ROC and chi‑square test. The association of LINC01270 with the CT signs and their combined effects in the diagnosis in glioma were also estimated. Serum LINC01270 was significantly elevated in glioma patients, which was closely associated with patients’ advanced WHO grades and lower KPS. Both LINC01270 upregulation and CT findings showed significant potential in diagnosing glioma, and LINC01270 correlated significantly with the invasion risk and metastasis indicated on CT. The combination of LINC01270 expression and CT findings significantly improved the sensitivity and specificity of glioma diagnosis. Upregulated LINC01270 in glioma is associated with malignant and severe disease development and has significant diagnostic value. Combined detection of LINC01270 and CT findings could improve the diagnostic efficacy in glioma cases, thus optimizing clinical diagnosis.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"197 3","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140443504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of morphine on conditioned place preference and pain are independent of uptake‑2 吗啡对条件性场所偏好和疼痛的影响与摄取-2 无关

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-14 DOI: 10.55782/ane-2024-2517

Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh

{"title":"Effects of morphine on conditioned place preference and pain are independent of uptake‑2","authors":"Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh","doi":"10.55782/ane-2024-2517","DOIUrl":"https://doi.org/10.55782/ane-2024-2517","url":null,"abstract":"Morphine changes neurotransmitter release, including norepinephrine, dopamine, and serotonin. Decynium‑22 (D22) inhibits an alternative neurotransmitter removal pathway, namely uptake‑2. Uptake‑2 includes plasma membrane monoamine transporter (PMAT) and organic cation transporters that have a low affinity, but high capacity for uptake of various monoamines such as norepinephrine, dopamine, and serotonin. This study was done to assess the effect of uptake‑2 inhibition on morphine‑induced conditioned place preference (CPP) and analgesia. In this study, the effects of morphine and/or D22 on CPP were evaluated following intraperitoneal injection in mice. Afterward, changes in motor activity were evaluated by the open field test. Using the tail‑flick model, the effects of D22 and/or morphine were evaluated on the pain threshold. The results showed that 20 mg/kg of morphine induced a place preference response. D22, at the dose of 0.03 mg/kg, caused place avoidance, while at the dose of 0.3 mg/kg, it produced a notable place preference response. Co‑administration of D22 and morphine showed that morphine reversed the CPP aversion induced by D22 at the lowest dose. Motor activity did not alter. In the tail‑flick test, morphine, at the dose of 3 mg/kg but not 1 mg/kg, increased the pain threshold. D22 induced significant analgesic responses. Co‑administration of D22 and morphine caused considerable analgesic effects. The findings revealed that D22 induced both conditioned aversion and preference depending on the dose while morphine induced CPP. Both drugs produced analgesia.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"51 5","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139779078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of morphine on conditioned place preference and pain are independent of uptake‑2 吗啡对条件性场所偏好和疼痛的影响与摄取-2 无关

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-14 DOI: 10.55782/ane-2024-2517

Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh

{"title":"Effects of morphine on conditioned place preference and pain are independent of uptake‑2","authors":"Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh","doi":"10.55782/ane-2024-2517","DOIUrl":"https://doi.org/10.55782/ane-2024-2517","url":null,"abstract":"Morphine changes neurotransmitter release, including norepinephrine, dopamine, and serotonin. Decynium‑22 (D22) inhibits an alternative neurotransmitter removal pathway, namely uptake‑2. Uptake‑2 includes plasma membrane monoamine transporter (PMAT) and organic cation transporters that have a low affinity, but high capacity for uptake of various monoamines such as norepinephrine, dopamine, and serotonin. This study was done to assess the effect of uptake‑2 inhibition on morphine‑induced conditioned place preference (CPP) and analgesia. In this study, the effects of morphine and/or D22 on CPP were evaluated following intraperitoneal injection in mice. Afterward, changes in motor activity were evaluated by the open field test. Using the tail‑flick model, the effects of D22 and/or morphine were evaluated on the pain threshold. The results showed that 20 mg/kg of morphine induced a place preference response. D22, at the dose of 0.03 mg/kg, caused place avoidance, while at the dose of 0.3 mg/kg, it produced a notable place preference response. Co‑administration of D22 and morphine showed that morphine reversed the CPP aversion induced by D22 at the lowest dose. Motor activity did not alter. In the tail‑flick test, morphine, at the dose of 3 mg/kg but not 1 mg/kg, increased the pain threshold. D22 induced significant analgesic responses. Co‑administration of D22 and morphine caused considerable analgesic effects. The findings revealed that D22 induced both conditioned aversion and preference depending on the dose while morphine induced CPP. Both drugs produced analgesia.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"608 ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glymphatic system and aquaporin‑4 in epilepsy. 癫痫中的淋巴系统和水传导蛋白-4。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-12-29 DOI: 10.55782/ane-2023-2498

Dorota Nowicka

引用次数: 0

Pylabianca: comprehensive and user‑friendly Python package for single‑neuron data analysis. Pylabianca：用于单神经元数据分析的全面且用户友好的 Python 软件包。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-12-28 DOI: 10.55782/ane-2023-2440

Mikołaj Magnuski, Władysław Średniawa, Katarzyna Paluch, Davor Ivanovski, Harish Babu, Jan Kaminski

引用次数: 0

Molecular mechanisms and treatment strategies for methamphetamine‑induced neurodegeneration, inflammation and neurotoxicity. 甲基苯丙胺诱导的神经变性、炎症和神经毒性的分子机制和治疗策略。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-12-28 DOI: 10.55782/ane-2023-2488

Samareh Omidvari, Zahra Azimzadeh, Fariborz Rashnoo, Foozhan Tahmasebinia, Aliasghar Keramatinia, Navid Ahmady Roozbahany, Hojjat Allah Abbaszadeh, Shahram Darabi

{"title":"Molecular mechanisms and treatment strategies for methamphetamine‑induced neurodegeneration, inflammation and neurotoxicity.","authors":"Samareh Omidvari, Zahra Azimzadeh, Fariborz Rashnoo, Foozhan Tahmasebinia, Aliasghar Keramatinia, Navid Ahmady Roozbahany, Hojjat Allah Abbaszadeh, Shahram Darabi","doi":"10.55782/ane-2023-2488","DOIUrl":"10.55782/ane-2023-2488","url":null,"abstract":"Methamphetamine (METH) is a highly addictive psychostimulant known for its profound impact on the nervous system. Chronic METH use leads to neurotoxicity characterized by various molecular and structural alterations in the brain. This review article primarily aims to elucidate the mechanisms underlying METH‑induced neurotoxicity. METH's mechanism of action involves the inhibition of dopamine, serotonin, and norepinephrine reuptake, resulting in altered synaptic function. Prolonged METH exposure triggers oxidative stress, endoplasmic reticulum stress, mitochondrial dysfunction, impaired axonal transport, autophagy, and programmed cell death, ultimately contributing to neurotoxicity. These neurotoxic effects manifest as increased neuronal firing rate, disruptions in intracellular ion balance (Ca2+ and Na+), energy production imbalances, and excessive reactive oxygen species production. The blood‑brain barrier is compromised, leading to structural, functional, and neurochemical alterations, particularly in the fronto‑striatal circuit. While our comprehensive review addresses these intricate molecular and structural changes induced by METH, we also examined the latest therapeutic strategies designed to mitigate neurotoxicity. Our investigation sheds light on the critical need to comprehend the complex pathways underlying METH‑induced neurotoxicity and develop effective treatment approaches.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 4","pages":"414-431"},"PeriodicalIF":1.4,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139465822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early development of the Tsc1 Purkinje cell specific mouse knockouts. Tsc1浦肯野细胞特异性基因敲除小鼠的早期发育。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2023-12-12 DOI: 10.55782/ane-2023-2472

Anna Sługocka, Marta Anna Przybyła, Jarosław Jerzy Barski

{"title":"Early development of the Tsc1 Purkinje cell specific mouse knockouts.","authors":"Anna Sługocka, Marta Anna Przybyła, Jarosław Jerzy Barski","doi":"10.55782/ane-2023-2472","DOIUrl":"10.55782/ane-2023-2472","url":null,"abstract":"Tsc1 is a gene which expression results in hamartin, a protein involved in regulation of the mTOR1 pathway. Inactivation of Tsc1 gives rise to hyperactivation of the mTOR1 machinery, increased proliferation and growth of cells with subsequent cell degeneration and cell death. In humans, mutations of Tsc1 result in an inherited disorder ‑ tuberous sclerosis complex (TSC) with the concomitant multiorgan non‑malignant tumors (tubers), epileptic seizures and autistic‑like manifestations. General mouse knock‑outs, homozygous for the inactivated Tsc1 alleles do not survive and die at early embryonal stages. To circumvent this problem, we utilized the Cre/loxP system and removed Tsc1 specifically in Purkinje cells using the pcp2/L7Cre mouse strain and the Tsc1tmDjk/J strains. Because of the published results showing the autistic‑like symptoms after the same crossbred, we have decided to look closer at the early postnatal period of these mutants. Surprisingly no evidence of any behavioral alterations were found, including the ultrasonic vocalizations of newborns. We decided to focus more attention on the interpretation of data, including a more detailed statistical evaluation of our results.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"83 4","pages":"404-413"},"PeriodicalIF":1.4,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139465794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0