Acta neurobiologiae experimentalis最新文献_第10页

Comprehensive analysis of lncRNA‑miRNA‑mRNA regulatory networks for Alzheimer's disease. 阿尔茨海默病lncRNA - miRNA - mRNA调控网络的综合分析。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-025

Fenghua Li, Zaihong Lin, Gengsheng Tian

{"title":"Comprehensive analysis of lncRNA‑miRNA‑mRNA regulatory networks for Alzheimer's disease.","authors":"Fenghua Li, Zaihong Lin, Gengsheng Tian","doi":"10.55782/ane-2022-025","DOIUrl":"https://doi.org/10.55782/ane-2022-025","url":null,"abstract":"Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative disease, associated with a decreased cognitive function and severe behavioral abnormalities. This study aimed to explore mechanisms of development and progression of AD. Comprehensive analysis of GSE16759 was performed to identify the differentially expressed lncRNAs (DElncRNAs), miRNAs (DEmiRNAs), and mRNAs (DEmRNAs). The differentially expressed RNAs (DERs) were used for the subsequent analysis, including module genes analysis, pathway enrichment analysis, and interaction network analysis. Finally, an AD‑associated network consisting of lncRNA‑miRNA‑mRNA‑pathway was constructed. A total of 431 DEmRNAs, 35 DElncRNAs, and 103 DEmiRNAs between the AD group and the normal control group were identified. DEmRNAs were significantly enriched in 13 pathways, such as focal adhesion, endocytosis, and mTOR signaling pathway. Three modules significantly related to AD were finally screened. The AD‑associated network was constructed, including 2 lncRNAs (A2M‑AS1 and ZNF571‑AS1), 1 miRNA (hsa‑miR‑206), 2 mRNAs (NOTCH3 and JAG1), and 2 pathways (notch signaling pathway and endocrine resistance). A2M‑AS1, ZNF571‑AS1, hsa‑miR‑206, NOTCH3 and JAG1 may be involved in the mechanisms of AD through notch signaling pathway and endocrine resistance.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 3","pages":"263-272"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33498382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Opioidergic and nitrergic systems mediate the anticonvulsant effect of mefloquine and chloroquine on seizures induced by pentylenetetrazol and maximal electroshock in mice. 阿片能和硝能系统介导甲氟喹和氯喹对戊四氮诱导的小鼠癫痫发作和最大电击的抗惊厥作用。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-014

Saina Saadat Boroujeni, Shahabaddin Solaimanian, Razieh Mohammad Jafari, Omid Sabzevari, Hamed Shafaroodi, Adeleh Maleki, Parsa Mohammadi, Elaheh Karimi, Ahmad Reza Dehpour

{"title":"Opioidergic and nitrergic systems mediate the anticonvulsant effect of mefloquine and chloroquine on seizures induced by pentylenetetrazol and maximal electroshock in mice.","authors":"Saina Saadat Boroujeni, Shahabaddin Solaimanian, Razieh Mohammad Jafari, Omid Sabzevari, Hamed Shafaroodi, Adeleh Maleki, Parsa Mohammadi, Elaheh Karimi, Ahmad Reza Dehpour","doi":"10.55782/ane-2022-014","DOIUrl":"10.55782/ane-2022-014","url":null,"abstract":"This study was designed to investigate the involvement of opioidergic/nitrergic systems in the anticonvulsant effect of mefloquine, compared with chloroquine, in mice. Seizures were induced by pentylenetetrazol and maximal electroshock. Mice were randomly subjected to receive mefloquine or chloroquine thirty minutes in advance. The role of opioidergic/nitrergic systems was shown by co‑administration of pharmacological intervention and nitrite levels measurement in mice hippocampi. Results indicated that mefloquine (40 mg/kg) and chloroquine (5 mg/kg) significantly decreased the occurrence of tonic hindlimb extension. Also, mefloquine 120 mg/kg and chloroquine 5 mg/kg significantly increased seizure latency and decreased mortality rate. Mefloquine decreased seizure frequency too. Besides, mefloquine (20 mg/kg) and chloroquine (5, 10 mg/kg) significantly increased seizure threshold. Interestingly, L‑NAME, 7‑NI and naltrexone pre‑treatment reversed the anticonvulsant effects of both mefloquine (20 mg/kg) and chloroquine (5 mg/kg). Moreover, co‑administration of minimal‑effective doses of morphine with mefloquine/chloroquine (both 1 mg/kg) potentiated anticonvulsant effects, which was reversed by naltrexone and endorsed the involvement of opioid receptors. Also, nitrite levels in mice hippocampi remarkably increased after treatment with both mefloquine (20 mg/kg) and chloroquine (5 mg/kg). To conclude, mefloquine could protect the central nervous system against seizures in PTZ/MES‑induced models through opioidergic/nitrergic pathways, with similarity to chloroquine effects.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" ","pages":"157-169"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40615346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of intranasal administration of caffeine on mPFC ischemia‑induced cognitive impairment in BALB/c mice. 鼻内给药咖啡因对BALB/c小鼠mPFC缺血引起的认知障碍的影响。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-028

Fatemeh Farokhi-Sisakht, Mehdi Farhoudi, Javad Mahmoudi, Fereshteh Farajdokht, Rana Kahfi-Ghaneh, Saeed Sadigh-Eteghad

{"title":"Effect of intranasal administration of caffeine on mPFC ischemia‑induced cognitive impairment in BALB/c mice.","authors":"Fatemeh Farokhi-Sisakht, Mehdi Farhoudi, Javad Mahmoudi, Fereshteh Farajdokht, Rana Kahfi-Ghaneh, Saeed Sadigh-Eteghad","doi":"10.55782/ane-2022-028","DOIUrl":"https://doi.org/10.55782/ane-2022-028","url":null,"abstract":"Caffeine is a psychoactive compound used widely to enhance cognitive functions in human or animal studies. The present study examined the effects of caffeine on cognitive performance and inflammatory factors in mice with medial prefrontal cortex (mPFC) ischemia. Mice underwent a photothrombotic mPFC ischemic stroke and were treated with normal saline or caffeine at different doses intranasally for 7 days. The sham surgery animals received normal saline intranasally. The Morris water maze test and social interaction test were performed to assess spatial and social memories, respectively. In addition, the levels of inflammatory proteins, including tumor necrosis factor‑alpha, interleukin‑6, and interleukin‑10, were measured in the mPFC using immunoblotting. The results showed that mPFC ischemia impaired spatial memory and social behaviors, and caffeine at doses of 0.05 and 0.1 mg improved behavioral outcomes in the ischemic groups. Also, caffeine reversed ischemia‑induced high levels of pro‑inflammatory biomarkers and enhanced the expression of the anti‑inflammatory mediator. Our findings indicate that caffeine alleviated mPFC ischemia‑induced memory disturbances, probably through the modulation of the inflammatory mediators.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 3","pages":"295-303"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33499397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Study on the molecular mechanism of Guipi decoction against sleep deprivation based on integrated pharmacology analysis and gene expression profiling. 基于综合药理学分析和基因表达谱的桂皮汤抗睡眠剥夺分子机制研究。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-039

Xu He, Xia Du, Jun Chen

{"title":"Study on the molecular mechanism of Guipi decoction against sleep deprivation based on integrated pharmacology analysis and gene expression profiling.","authors":"Xu He, Xia Du, Jun Chen","doi":"10.55782/ane-2022-039","DOIUrl":"https://doi.org/10.55782/ane-2022-039","url":null,"abstract":"Sleep disorder is a puzzling and complex health problem, and sleep deprivation (SD) may be a window for studying sleep disorder. Guipi decoction (GPD) is a classic Chinese prescription for the treatment of sleep disorder. However, the mechanism of GPD remains puzzling. In this paper, integrated pharmacological analysis and gene expression profiling were introduced to study the mechanism of GPD in treatment with SD. Firstly, the integrative pharmacology‑based research platform of traditional Chinese medicine (TCMIP) was applied to collect chemical compounds and corresponding targets for GPD. Secondly, SD‑related targets were obtained by gene expression profiling (GSE56931) from Gene Expression Omnibus (GEO) database. The String database screened the core targets according to protein‑protein interaction (PPI) network. Furthermore, kyoto encyclopedia of genes and genomes (KEGG) pathways were carried out based on the Database for Annotation, Visualization and Integrated Discovery (DAVID) database. Conclusively, the \"formula‑herbs‑compounds‑targets‑pathways\" network was established to explore the mechanism of GPD in the treatment of SD. In addition, molecular docking was carried out to verify the connection between hub compounds and targets. The results showed that GPD was mainly linked to 44 compounds, 19 targets and 5 pathways. GPD in the treatment of sleep deprivation through metabolic pathways and cAMP signaling pathway, which were related to NR3C1, MAPK3, PPARA and core compounds such as adenosine. This study preliminarily revealed the molecular mechanism of GPD for SD, and lays a foundation for the study of the mechanism against SD for GPD.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 4","pages":"409-423"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10725211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Intra‑LPGi microinjection of glutamate receptors antagonists abolish 17β‑estradiol‑induced analgesic effect in the ovariectomized rats. 谷氨酸受体拮抗剂在去卵巢大鼠LPGi内微注射可消除17β -雌二醇诱导的镇痛作用。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-050

Hanieh Feyzi, Fatemeh Khakpai, Roghaieh Khakpay, Farzam Sheikhzadeh Hesari, Saeed Semnanian

{"title":"Intra‑LPGi microinjection of glutamate receptors antagonists abolish 17β‑estradiol‑induced analgesic effect in the ovariectomized rats.","authors":"Hanieh Feyzi, Fatemeh Khakpai, Roghaieh Khakpay, Farzam Sheikhzadeh Hesari, Saeed Semnanian","doi":"10.55782/ane-2022-050","DOIUrl":"https://doi.org/10.55782/ane-2022-050","url":null,"abstract":"This study was designed to investigate a possible interaction between 17β‑estradiol and glutamate receptors of the paragigantocellularis lateralis (LPGi) nucleus on pain coping behavior using the formalin test in ovariectomized (OVX) rats. The results showed that intra‑LPGi injection of 17β‑estradiol declined flexing behavior in both phases of the formalin test. Still, it only diminished the late phase of licking behavior in the OVX rats. NMDA receptor antagonist, AP5, reversed the analgesic effect of 17β‑estradiol on flexing behavior in both phases of the formalin test in the OVX rats. The 17β‑estradiol‑induced anti‑nociceptive effect on the flexing duration was prevented by CNQX (AMPA receptor antagonist) only in the early phase of the formalin test in the OVX rats. AP5 and CNQX reduced the anti‑nociceptive effect of 17β‑estradiol in the late phase, but not the early phase of licking response in the OVX rats. These results suggested: (i) The intra‑LPGi injection of 17β‑estradiol is satisfactory in producing modest analgesia on the formalin‑induced inflammatory pain in the OVX rats; (ii) Co‑treatment of glutamate receptors (NMDA and AMPA) antagonists and 17β‑estradiol in the LPGi nucleus decrease the analgesic effect of 17β‑estradiol in the OVX rats; (iii) There is a possible association between 17β‑estradiol and glutamate receptors of the LPGi nucleus on pain coping behavior in the OVX rats.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 4","pages":"521-533"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10725212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of social hierarchy on innate fear‑induced panic responses. 社会等级对先天恐惧引起的恐慌反应的影响。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-012

Soomaayeh Heysieattalab, Roghaieh Khakpay, Mahshad Fadaeimoghadam Heydarabadi, Maryam Aboureihani Mohammadi, Soheila Hashemi, Fatemeh Bagheri

{"title":"Effects of social hierarchy on innate fear‑induced panic responses.","authors":"Soomaayeh Heysieattalab, Roghaieh Khakpay, Mahshad Fadaeimoghadam Heydarabadi, Maryam Aboureihani Mohammadi, Soheila Hashemi, Fatemeh Bagheri","doi":"10.55782/ane-2022-012","DOIUrl":"https://doi.org/10.55782/ane-2022-012","url":null,"abstract":"Studies have previously demonstrated a relationship between social status and anxiety disorders such as panic disorder. Repeated episodes of panic attacks do not occur in combination with an actual fear stimulus or stressor. However, social ranking modulates the perception of the social signals of a threat or stressor. The hypothalamic nuclei are well‑known for their role in the elaboration of fear‑induced reactions. The dorsomedial hypothalamus (DMH) and the ventromedial hypothalamic (VMH) nuclei are hypothalamic subnuclei involved in the processing of threatening stimuli‑evoked aversive response and innate fear development. These structures are also located in the medial amygdala‑hypothalamus‑brainstem circuit that modulates innate fear‑induced defensive behaviors. This work aimed to investigate the relationship between social hierarchy and innate fear‑induced panic‑like responses in male rats. In our study, the dominance tube test was used to determine the social hierarchy. Then, DMH/VMH nuclei were unilaterally implanted with a guide cannula. After intra‑DMH/VMH injection of bicuculline (GABAA receptor antagonist), both innate fear induction and differences in dominant/subordinate rats were evaluated by the open field test. Intra‑DMH/VMH bicuculline increased the frequency of defensive immobility, forward escape movements, and crossing behaviors, as well as the duration of defensive immobility and forward escape movements in dominant rats. Subordinate rats showed a higher frequency of defensive attention, defensive immobility, and crossing than dominant rats. Additionally, dominant rats demonstrated a lower duration of defensive attention and defensive immobility than subordinate rats. Dominant rats seemed to adopt a form of innate‑fear characterized by increased proactivity with the environment. In contrast, subordinate rats exhibited a reactive form of innate‑fear characterized by passivity and freezing.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 2","pages":"133-146"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10812702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Serum biomarkers based neurotrauma severity scale: a study in the mice model of fluid percussion injury. 基于血清生物标志物的神经损伤严重程度量表:液体冲击损伤小鼠模型的研究。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-013

Mohd Aleem, Nidhi Goswami, Kailash Manda

{"title":"Serum biomarkers based neurotrauma severity scale: a study in the mice model of fluid percussion injury.","authors":"Mohd Aleem, Nidhi Goswami, Kailash Manda","doi":"10.55782/ane-2022-013","DOIUrl":"https://doi.org/10.55782/ane-2022-013","url":null,"abstract":"The study aimed to investigate the significance of serum biomarkers in the severity grading of traumatic brain injury (TBI). For this purpose, mice underwent fluid percussion injury (FPI) at three discrete severity levels, mild, moderate, and severe. The severity of trauma was verified by the qualitative and quantitative histopathology of the brain. The serum samples were analyzed for the potential changes in ubiquitin C‑terminal hydrolase‑1 (UCHL‑1), S100β, interleukin‑6 (IL‑6), corticosterone, and β‑endorphin at 24 and 72 h post injury. A multifold increase in the values of UCHL‑1 was reported at all severity extents of FPI. However, TBI severity‑dependent increase in UCHL‑1 was reported on 72 h following FPI but not at 24 h. S100β values were significantly augmented in the mild and moderate group at both the time point but not in the severe group. Serum level of IL‑6 was significantly increased in the mild injury group at 24 h but not in the moderate and severe. At 72 h, IL‑6 showed a reverse trend. β‑endorphin and corticosterone were sensitive at an early stage only. Such unique dynamics of each biomarker enable us to propose TBI severity scale in the term of biomarkers codes to predict the extent of neurotrauma. Our preclinical study presents a predictive model for further clinical validation.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" ","pages":"147-156"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40615345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repeated acetaminophen administration damaged hippocampal tissue but did not affect prefrontal cortex or anxiety behaviors. 反复给药对乙酰氨基酚损伤海马组织，但不影响前额叶皮层或焦虑行为。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-015

Asli Karakilic, Servet Kizildag, Zeynep Yuce, Yasemin Seval Celik, Sevim Kandis, Hemdem Rodi Bozan, Basar Koc, Guven Guvendi, Mehmet Ates, Sevinc Inan, Nazan Uysal

{"title":"Repeated acetaminophen administration damaged hippocampal tissue but did not affect prefrontal cortex or anxiety behaviors.","authors":"Asli Karakilic, Servet Kizildag, Zeynep Yuce, Yasemin Seval Celik, Sevim Kandis, Hemdem Rodi Bozan, Basar Koc, Guven Guvendi, Mehmet Ates, Sevinc Inan, Nazan Uysal","doi":"10.55782/ane-2022-015","DOIUrl":"https://doi.org/10.55782/ane-2022-015","url":null,"abstract":"Acetaminophen is one of the most widely used over‑the‑counter drugs worldwide for the treatment of pain and fever. Although acetaminophen use is known to impair hippocampus‑related learning and memory, its effect on anxiety is not clear. Insulin‑like growth factor‑1 (IGF‑1) and matrix metalloproteinase‑2 (MMP2) are important for cellular survival, maintenance and tissue integrity. The aim of this study was to investigate the dose‑dependent effects of acetaminophen on anxiety levels as well as on hippocampus, prefrontal cortex and liver tissue. Doses of 100, 200 and 400 mg/kg acetaminophen were administered to male Sprague Dawley rats for 11 days and anxiety tests were conducted on the last day. Twenty‑four hours after the last acetaminophen administration, all animals were sacrificed and hippocampus, prefrontal cortex and liver tissues were removed for analyses. Hippocampal IGF‑1 and MMP2 levels were shown to decrease only at the highest dose of acetaminophen, which was accompanied by pathological changes in histology. The prefrontal cortex was not affected. Behavioral analyses also did not indicate changes in anxiety levels in the rats. Liver IGF‑1 and MMP2 levels decreased in all experimental groups. Serum alanine aminotransferase and aspartate aminotransferase levels increased in the 200 mg/kg and 400 mg/kg acetaminophen groups. Our findings showed that varying doses of acetaminophen did not affect the prefrontal cortex or anxiety levels. Further research is needed to elucidate the hippocampal and hepatic protective roles of IGF‑1 and MMP2 in acetaminophen toxicity and their potential use in therapeutic approaches.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" ","pages":"170-178"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40615783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of potential biomarkers and small‑molecule compounds related to intracerebral hemorrhage with bioinformatics analysis. 用生物信息学分析鉴定与脑出血相关的潜在生物标志物和小分子化合物。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-017

Ziqi Yang, Ruonan Wang, Xingyu Chen, Dexi Zhao

{"title":"Identification of potential biomarkers and small‑molecule compounds related to intracerebral hemorrhage with bioinformatics analysis.","authors":"Ziqi Yang, Ruonan Wang, Xingyu Chen, Dexi Zhao","doi":"10.55782/ane-2022-017","DOIUrl":"https://doi.org/10.55782/ane-2022-017","url":null,"abstract":"This study aimed to further explore the underlying molecular mechanism of intracerebral hemorrhage (ICH), gene expression profile GSE24265, containing perihematomal tissues, contralateral grey and white matters were retrieved and analyzed. The data was hierarchically clustered and the differentially expressed genes (DEGs) were screened. Functional analysis and protein interaction analysis of DEG hubs were performed, and the miRNA‑transcription factor (TF)‑target network was built. In addition, the candidate small-molecule compounds that might reverse the expression of an ICH‑linked gene were identified by CMap. This method revealed a total of 408 DEGs. Five modules including chemokinerelated, antigen immune-related, pathogen infection, cell reaction, and positive regulation of tyrosine phosphorylation and MAPK cascade were identified. The expression levels of CCL5, CXCL8, ICAM1, IL-1B, IL-6, VCAM1, and VEGFA were correlated with ICH among the top 10 hub genes obtained in the protein-protein interaction (PPI) network. A total of 237 miRNA‑TF‑target regulatory relationships were obtained, including 6 TFs, 11 miRNAs and 105 target genes. Finally, the CMap database identified Prestwick-1083, xamoterol, ifosfamide, methyldopate, nifurtimox, propranolol, and methoxamine as potential therapeutic agents for ICH while doxorubicin, menadione and azacitidine may increase its pathogenicity. Furthermore, CCL5, CXCL8 and VEGFA may be novel candidate susceptibility genes for ICH. Some small-molecule drugs, including xamoterol may be used for the treatment of ICH.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" ","pages":"187-196"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40615785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Physical exercise and flaxseed oil supplementation influence the glial plasticity in the rat hippocampus. 体育锻炼和补充亚麻籽油对大鼠海马神经胶质可塑性的影响。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI: 10.55782/ane-2022-043

Karina Maia Paiva, Rodrigo Freire Oliveira, Livia Helena Morais de Freitas, Acydália Madruga de Mendonça Florêncio de Melo, Gabriel Sousa da Rocha, Kalina Fernandes Freire, Jeferson de Souza Cavalcante, Paulo Leonardo Araújo de Gois Morais, José Rodolfo Lopes de Paiva Cavalcanti

{"title":"Physical exercise and flaxseed oil supplementation influence the glial plasticity in the rat hippocampus.","authors":"Karina Maia Paiva, Rodrigo Freire Oliveira, Livia Helena Morais de Freitas, Acydália Madruga de Mendonça Florêncio de Melo, Gabriel Sousa da Rocha, Kalina Fernandes Freire, Jeferson de Souza Cavalcante, Paulo Leonardo Araújo de Gois Morais, José Rodolfo Lopes de Paiva Cavalcanti","doi":"10.55782/ane-2022-043","DOIUrl":"https://doi.org/10.55782/ane-2022-043","url":null,"abstract":"Brain benefits from physical exercise associated with antioxidant supplements such as flaxseed oil. This low cost and simple association may improve hippocampal plasticity, which may work as a preventive and effective therapy in neuroprotection and neuroplasticity processes. This work evaluated the effects of physical exercise with flaxseed oil supplementation (Linum usitatissimum L.) in the hippocampus of Wistar rats. We separated male Wistar rats into four experimental groups: control group (sedentary), a sedentary group with a supplemental diet of flaxseed oil, a group under exercise program with flaxseed oil supplementation, and a group exclusively under exercise program. The swimming exercise consisted of a progressive 28‑day protocol followed by behavioral assessment, brain perfusion, microtomy, immunohistochemistry for glial fibrillary acidic protein (GFAP), cellular morphology, and optical density analysis. We used the ANOVA test with Tukey's post‑test for behavioral analysis. The exercise program with flaxseed oil supplementation was able to alter the GFAP expression in astrocytes in the CA1, CA3 and dentate gyrus regions of the hippocampus and modulate the behavioral aspects of memory and anxiety.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"82 4","pages":"448-461"},"PeriodicalIF":1.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10692757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0