Effect of intranasal administration of caffeine on mPFC ischemia‑induced cognitive impairment in BALB/c mice.

IF 1.4 4区医学 Q4 NEUROSCIENCES

Acta neurobiologiae experimentalis Pub Date : 2022-01-01 DOI:10.55782/ane-2022-028

Fatemeh Farokhi-Sisakht, Mehdi Farhoudi, Javad Mahmoudi, Fereshteh Farajdokht, Rana Kahfi-Ghaneh, Saeed Sadigh-Eteghad

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引用次数: 2

Abstract

Caffeine is a psychoactive compound used widely to enhance cognitive functions in human or animal studies. The present study examined the effects of caffeine on cognitive performance and inflammatory factors in mice with medial prefrontal cortex (mPFC) ischemia. Mice underwent a photothrombotic mPFC ischemic stroke and were treated with normal saline or caffeine at different doses intranasally for 7 days. The sham surgery animals received normal saline intranasally. The Morris water maze test and social interaction test were performed to assess spatial and social memories, respectively. In addition, the levels of inflammatory proteins, including tumor necrosis factor‑alpha, interleukin‑6, and interleukin‑10, were measured in the mPFC using immunoblotting. The results showed that mPFC ischemia impaired spatial memory and social behaviors, and caffeine at doses of 0.05 and 0.1 mg improved behavioral outcomes in the ischemic groups. Also, caffeine reversed ischemia‑induced high levels of pro‑inflammatory biomarkers and enhanced the expression of the anti‑inflammatory mediator. Our findings indicate that caffeine alleviated mPFC ischemia‑induced memory disturbances, probably through the modulation of the inflammatory mediators.

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鼻内给药咖啡因对BALB/c小鼠mPFC缺血引起的认知障碍的影响。

咖啡因是一种精神活性化合物，在人类或动物研究中被广泛用于增强认知功能。本研究探讨了咖啡因对内侧前额叶皮质缺血小鼠认知能力和炎症因子的影响。小鼠接受光血栓性mPFC缺血性中风，用不同剂量的生理盐水或咖啡因鼻内治疗7天。假手术动物鼻内注射生理盐水。Morris水迷宫测验和社会互动测验分别评估空间记忆和社会记忆。此外，使用免疫印迹法测量mPFC中炎症蛋白的水平，包括肿瘤坏死因子- α、白细胞介素- 6和白细胞介素- 10。结果表明，mPFC缺血对大鼠空间记忆和社会行为造成损害，0.05和0.1 mg咖啡因可改善缺血组的行为结果。此外，咖啡因逆转了缺血诱导的高水平促炎生物标志物，并增强了抗炎介质的表达。我们的研究结果表明，咖啡因减轻了mPFC缺血引起的记忆障碍，可能是通过调节炎症介质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta neurobiologiae experimentalis 医学-神经科学

CiteScore

2.20

自引率

7.10%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Neurobiologiae Experimentalis (ISSN: 0065-1400 (print), eISSN: 1689-0035) covers all aspects of neuroscience, from molecular and cellular neurobiology of the nervous system, through cellular and systems electrophysiology, brain imaging, functional and comparative neuroanatomy, development and evolution of the nervous system, behavior and neuropsychology to brain aging and pathology, including neuroinformatics and modeling.