Acta neurobiologiae experimentalis最新文献_第4页

The effect of morphine administration on GluN3B NMDA receptor subunit mRNA expression in rat brain 吗啡对大鼠脑内 GluN3B NMDA 受体亚基 mRNA 表达的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-26 DOI: 10.55782/ane-2024-2545

Shahrzad Nazari, Mitra-Sadat Sadat-Shirazi, Ali Shahbazi, K. Ghaffari, N. Vousooghi

{"title":"The effect of morphine administration on GluN3B NMDA receptor subunit mRNA expression in rat brain","authors":"Shahrzad Nazari, Mitra-Sadat Sadat-Shirazi, Ali Shahbazi, K. Ghaffari, N. Vousooghi","doi":"10.55782/ane-2024-2545","DOIUrl":"https://doi.org/10.55782/ane-2024-2545","url":null,"abstract":"Opioid addiction is critically dependent on the activation of N‑methyl‑D‑aspartate (NMDA) receptors, which are widely found in the mesocorticolimbic system. Meanwhile, opioid addiction may affect the expression level of NMDA receptor subunits. The existence of GluN3 subunits in the NMDA receptor’s tetramer structure reduces the excitatory current of the receptor channel. We evaluated the changes in the mRNA expression pattern of the GluN3B subunit of the NMDA receptor in rat brains following acute and chronic exposure to morphine. Chronic, escalating intraperitoneal doses of morphine or saline were administered twice daily to male Wistar rats for six days. Two other groups were injected with a single acute dose of morphine or saline. The mRNA level of the GluN3B subunit of the NMDA receptor in the striatum, hippocampus, and nucleus accumbens (NAc) was measured by real‑time PCR. mRNA expression of the GluN3B subunit was considerably augmented (3.15 fold) in the NAc of animals chronically treated with morphine compared to the control group. The difference between rats that were chronically administered morphine and control rats was not statistically significant for other evaluated brain areas. In rats acutely treated with morphine, no significant differences were found for GluN3B subunit expression in the examined brain regions compared to the control group. It was concluded that chronic exposure to morphine notably increased the GluN3B subunit of the NMDA receptor in NAc. The extent of the impact of this finding on opioid addiction and its features requires further evaluation in future studies.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"101 2","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of neurogranin levels in a rat model of diffuse axonal injury 评估弥漫性轴突损伤大鼠模型中的神经粒蛋白水平

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-25 DOI: 10.55782/ane-2024-2543

Ozan Başkurt, Seckin Aydin, I. Avci, Pinar Ozay Nayir, M. V. Aydin

{"title":"Evaluation of neurogranin levels in a rat model of diffuse axonal injury","authors":"Ozan Başkurt, Seckin Aydin, I. Avci, Pinar Ozay Nayir, M. V. Aydin","doi":"10.55782/ane-2024-2543","DOIUrl":"https://doi.org/10.55782/ane-2024-2543","url":null,"abstract":"Diffuse axonal injury (DAI), one of the most common and devastating type of traumatic brain injury, is the result of the shear force on axons due to severe rotational acceleration and deceleration. Neurogranin (NRGN) is a postsynaptic protein secreted by excitatory neurons, and synaptic dysfunction can alter extracellular NRGN levels. In this study, we examined NRGN levels in serum and cerebrospinal fluid (CSF) after experimental DAI in terms of their diagnostic value. Experimental DAI was induced using the Marmarou technique in male Wistar albino rats. Serum and CSF NRGN levels of the sham group, one‑hour, six‑hour, 24‑hour, and 72‑hour post‑DAI groups were measured by ELISA method. DAI was verified by staining with hematoxylin‑eosin and β‑amyloid precursor protein in the rat brain samples. While no histopathological and immunohistochemical changes were observed in the early hours of the post‑DAI groups, the staining of the β‑APP visibly increased over time, with positivity being most frequent and intense in the 72‑hour group. It was found that serum NRGN levels were significantly lower in the 6‑hour group than in the sham group. The serum NRGN levels in the 24‑hour group were significantly higher than those in the sham group. This study showed a dichotomy of post‑DAI serum NRGN levels in consecutive time periods. NRGN levels in CSF were higher in the one‑hour group than in the sham group and returned to baseline by 72 hours, although not significantly. Our study provides an impression of serum and CSF NRGN levels in a rat DAI model in consecutive time periods. Further studies are needed to understand the diagnostic value of NRGN.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" December","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time‑dependent changes in blood cells, NIHSS and mRS according to reperfusion treatment type in stroke patients who developed hemorrhagic complication 发生出血性并发症的脑卒中患者的血细胞、NIHSS 和 mRS 随时间变化的再灌注治疗类型

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-22 DOI: 10.55782/ane-2024-2540

H. Kömürcü, E. Gözke, Irmak Salt, Isil Kalyoncu Aslan, Pelin Doğan Ak

{"title":"Time‑dependent changes in blood cells, NIHSS and mRS according to reperfusion treatment type in stroke patients who developed hemorrhagic complication","authors":"H. Kömürcü, E. Gözke, Irmak Salt, Isil Kalyoncu Aslan, Pelin Doğan Ak","doi":"10.55782/ane-2024-2540","DOIUrl":"https://doi.org/10.55782/ane-2024-2540","url":null,"abstract":"Hemorrhagic complications may be seen following reperfusion therapy with rtPA and/or thrombectomy after acute ischemic stroke (AIS). Neutrophils, lymphocytes, and platelets have important roles in the inflammatory and immune responses that develop in these patients. We investigated time‑dependent changes in blood cells, NIHSS and mRS values according to type of reperfusion therapy in AIS patients who developed cerebral hemorrhage. In AIS patients who underwent rtPA and/or thrombectomy and developed cerebral hemorrhage within the first 24 hours after treatment, leukocyte, neutrophil, lymphocyte, platelet counts and their ratios were recorded on admission, 1st, 3rd, and 7th days. NIHSS values on admission, 3rd days and mRS values on admission, discharge, and the 3rd month were recorded. These values were compared according to the type of reperfusion therapy. Out of 436 AIS patients, rtPA was applied in 50.5%, thrombectomy in 28.2%, and rtPA+thrombectomy in 21.3%. Hemorrhage developed in 25.5% of the patients. Patients treated with thrombectomy had a greater rate of cerebral hemorrhage. Pre‑stroke mRS values were lower in all therapy types than mRS scores at discharge and the 3rd month. The NIHSS scores did not differ significantly in 3 days. Depending on the type of reperfusion treatment, there are a few time‑dependent significant changes observed in the blood cell counts and ratios. In conclusion, there is a relation between the type of reperfusion therapy and the time‑dependent changes in blood cells and ratios as well as mRS scores among AIS patients who have undergone rtPA and/or thrombectomy and developed cerebral hemorrhage.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 11","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventive effects of crocin, a natural compound from saffron, against nicotine‑induced oxidative stress and neurobehavioral disturbances 藏红花中的天然化合物黄花苷对尼古丁诱导的氧化应激和神经行为紊乱的预防作用

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-19 DOI: 10.55782/ane-2024-2538

M. Motaghinejad, M. Motevalian, Antoni Sureda

{"title":"Preventive effects of crocin, a natural compound from saffron, against nicotine‑induced oxidative stress and neurobehavioral disturbances","authors":"M. Motaghinejad, M. Motevalian, Antoni Sureda","doi":"10.55782/ane-2024-2538","DOIUrl":"https://doi.org/10.55782/ane-2024-2538","url":null,"abstract":"Nicotine is a psychostimulant that induces neurochemical and behavioral changes upon chronic administration, leading to neurodegenerative conditions associated with smoking. As of now, no preventive or therapeutic strategies are known to counteract nicotine‑induced neurodegeneration. In this study, we explore the neuroprotective effects of crocin, a bioactive agent commonly found in saffron – a spice derived from the flower of Crocus sativus – using a rat model. The dose‑dependent effects of crocin were evaluated in nicotine‑induced neurodegeneration and compared with a control group. Neurobehavioral changes, assessed through the elevated plus maze, the open field test, the forced swim test, and the Morris water maze, as well as oxidative stress in the hippocampus, were evaluated. Interestingly, nicotine administration resulted in depression, anxiety, and abnormal motor and cognitive functions, while crocin treatment protected the rat brain from these abnormalities. The beneficial effects of crocin were associated with reduced oxidative stress biomarkers such as malondialdehyde, along with increases in superoxide dismutase, glutathione peroxidase, and glutathione reductase activities. These results demonstrate that crocin can mitigate nicotine‑induced neurodegeneration by reducing oxidative stress, potentially offering a protective measure against neurodegenerative effects in smokers.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 7","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model 左乙拉西坦对颞叶癫痫模型中 DNA 氧化和谷胱甘肽含量的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-19 DOI: 10.55782/ane-2024-2537

Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez

{"title":"Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model","authors":"Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez","doi":"10.55782/ane-2024-2537","DOIUrl":"https://doi.org/10.55782/ane-2024-2537","url":null,"abstract":"Levetiracetam (LEV) is a drug commonly used as an anticonvulsant. However, recent evidence points to a possible role as an antioxidant. We previously demonstrated the antioxidant properties of LEV by significantly increasing catalase and superoxide dismutase activities and decreasing the hydrogen peroxide (H2O2) levels in the hippocampus of rats with temporal lobe epilepsy (TLE) showing scavenging properties against the hydroxyl radical. The aim of the present work was to evaluate, the effect of LEV on DNA oxidation, by determining 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG) levels, and glutathione content, through reduced (GSH) and oxidized (GSSG) glutathione levels, in the hippocampus of rats with TLE. Male Wistar rats were assigned to the control (CTRL), CTRL+LEV, epileptic (EPI) and EPI+LEV groups. TLE was induced using the lithium‑pilocarpine model. Thirteen weeks after TLE induction, LEV was administered for one week through osmotic pumps implanted subcutaneously. The determination of 8‑OHdG, GSH and GSSG levels were measured using spectrophotometric methods. We showed that LEV alone significantly increased 8‑OHdG and GSSG levels in the hippocampus of control rats compared to those in epileptic condition. No significant differences in GSH levels were observed. LEV could induce changes in the hippocampus increasing DNA oxidation and GSSG levels under nonepileptic condition but not protecting against the mitochondrial dysfunction observed in TLE probably by mechanisms related to changes in chromatin structure, neuroinflammation and alterations in redox components.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 2","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse 将本征信号光学成像作为描述幼鼠初级视觉皮层定向敏感性的工具

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-29 DOI: 10.55782/ane-2024-2397

I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel

{"title":"Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse","authors":"I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel","doi":"10.55782/ane-2024-2397","DOIUrl":"https://doi.org/10.55782/ane-2024-2397","url":null,"abstract":"We employed intrinsic signal optical imaging (ISOI) to investigate orientation sensitivity bias in the visual cortex of young mice. Optical signals were recorded in response to the moving light gratings stimulating ipsi‑, contra‑ and binocular eye inputs. ISOI allowed visualization of cortical areas activated by gratings of specific orientation and temporal changes of light scatter during visual stimulation. These results confirmed ISOI as a reliable technique for imaging the activity of large populations of neurons in the mouse visual cortex. Our results revealed that the contralateral ocular input activated a larger area of the primary visual cortex than the ipsilateral input, and caused the highest response amplitudes of light scatter signals to all ocular inputs. Horizontal gratings moved in vertical orientation induced the most significant changes in light scatter when presented contralaterally and binocularly, surpassing stimulations by vertical or oblique gratings. These observations suggest dedicated integration mechanisms for the combined inputs from both eyes. We also explored the relationship between point luminance change (PLC) of grating stimuli and ISOI time courses under various orientations of movements of the gratings and ocular inputs, finding higher cross-correlation values for cardinal orientations and ipsilateral inputs. These findings suggested specific activation of different neuronal assemblies within the mouse’s primary visual cortex by grating stimuli of the corresponding orientation. However, further investigations are needed to examine this summation hypothesis. Our study highlights the potential of optical imaging as a valuable tool for exploring functional‑anatomical relationships in the mouse visual system.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"15 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alarin potentiates ongoing epileptiform activity in rat brain slices: an in vitro electrophysiological study 阿拉林能增强大鼠大脑切片中的持续癫痫样活动：体外电生理学研究

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-21 DOI: 10.55782/ane-2024-2520

Ömer Faruk Kalkan, Hilal Öztürk, Zafer Şahin, Harun Başoğlu, Selcen Aydin Abidin, İ. Abidin

{"title":"Alarin potentiates ongoing epileptiform activity in rat brain slices: an in vitro electrophysiological study","authors":"Ömer Faruk Kalkan, Hilal Öztürk, Zafer Şahin, Harun Başoğlu, Selcen Aydin Abidin, İ. Abidin","doi":"10.55782/ane-2024-2520","DOIUrl":"https://doi.org/10.55782/ane-2024-2520","url":null,"abstract":"Alarin is a newly discovered neuropeptide that belongs to the galanin peptide family with a wide range of bioactivity in the nervous system. Its function in the brain’s autonomic areas has been studied, and it has been reported that alarin is involved in the regulation of excitability in hypothalamic neurons. Its role in the regulation of excitability in the hippocampus, however, is unknown. In this study, we investigated if alarin induced any synchronous discharges or epileptiform activity, and if it had any effect on already initiated epileptiform discharges. We used thick acute horizontal hippocampal slices obtained from 30‑ to 35‑day‑old rats. Extracellular field potential recordings were evaluated in the CA1 region of the hippocampus. Our data demonstrated that, alarin application did not result in any epileptiform activity or abnormal discharges. 4‑aminopyridine was applied to induce epileptiform activity in the slices. We found that alarin increased the frequency of interictal‑like events and the mean power of local field potentials in the CA1 region of the hippocampus, which was induced by 4‑aminopyridine. These results demonstrated for the first time that alarin has a modulatory effect on synchronized neuronal discharges and showed the contribution of the neuropeptide alarin to epilepsy‑like conditions.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"20 6","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140444422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abnormal expression of long non‑coding RNA LINC01270 in glioma and its correlation with X‑ray computed tomography signs 胶质瘤中长非编码 RNA LINC01270 的异常表达及其与 X 射线计算机断层扫描征象的相关性

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-21 DOI: 10.55782/ane-2024-2521

Ying Liu, Yuntao Zhang, Yongjun E, Xianglin Zhang, Heji Ma, Furen Dong

{"title":"Abnormal expression of long non‑coding RNA LINC01270 in glioma and its correlation with X‑ray computed tomography signs","authors":"Ying Liu, Yuntao Zhang, Yongjun E, Xianglin Zhang, Heji Ma, Furen Dong","doi":"10.55782/ane-2024-2521","DOIUrl":"https://doi.org/10.55782/ane-2024-2521","url":null,"abstract":"This study focused on the association of LINC01270 and computed tomography (CT) signs with glioma development, to evaluate their potential in the early detection of glioma. Serum LINC01270 was evaluated in glioma patients and healthy individuals using PCR. The involvement of LINC01270 in glioma onset and development was evaluated by ROC and chi‑square test. The association of LINC01270 with the CT signs and their combined effects in the diagnosis in glioma were also estimated. Serum LINC01270 was significantly elevated in glioma patients, which was closely associated with patients’ advanced WHO grades and lower KPS. Both LINC01270 upregulation and CT findings showed significant potential in diagnosing glioma, and LINC01270 correlated significantly with the invasion risk and metastasis indicated on CT. The combination of LINC01270 expression and CT findings significantly improved the sensitivity and specificity of glioma diagnosis. Upregulated LINC01270 in glioma is associated with malignant and severe disease development and has significant diagnostic value. Combined detection of LINC01270 and CT findings could improve the diagnostic efficacy in glioma cases, thus optimizing clinical diagnosis.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"197 3","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140443504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of morphine on conditioned place preference and pain are independent of uptake‑2 吗啡对条件性场所偏好和疼痛的影响与摄取-2 无关

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-14 DOI: 10.55782/ane-2024-2517

Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh

{"title":"Effects of morphine on conditioned place preference and pain are independent of uptake‑2","authors":"Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh","doi":"10.55782/ane-2024-2517","DOIUrl":"https://doi.org/10.55782/ane-2024-2517","url":null,"abstract":"Morphine changes neurotransmitter release, including norepinephrine, dopamine, and serotonin. Decynium‑22 (D22) inhibits an alternative neurotransmitter removal pathway, namely uptake‑2. Uptake‑2 includes plasma membrane monoamine transporter (PMAT) and organic cation transporters that have a low affinity, but high capacity for uptake of various monoamines such as norepinephrine, dopamine, and serotonin. This study was done to assess the effect of uptake‑2 inhibition on morphine‑induced conditioned place preference (CPP) and analgesia. In this study, the effects of morphine and/or D22 on CPP were evaluated following intraperitoneal injection in mice. Afterward, changes in motor activity were evaluated by the open field test. Using the tail‑flick model, the effects of D22 and/or morphine were evaluated on the pain threshold. The results showed that 20 mg/kg of morphine induced a place preference response. D22, at the dose of 0.03 mg/kg, caused place avoidance, while at the dose of 0.3 mg/kg, it produced a notable place preference response. Co‑administration of D22 and morphine showed that morphine reversed the CPP aversion induced by D22 at the lowest dose. Motor activity did not alter. In the tail‑flick test, morphine, at the dose of 3 mg/kg but not 1 mg/kg, increased the pain threshold. D22 induced significant analgesic responses. Co‑administration of D22 and morphine caused considerable analgesic effects. The findings revealed that D22 induced both conditioned aversion and preference depending on the dose while morphine induced CPP. Both drugs produced analgesia.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"51 5","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139779078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of morphine on conditioned place preference and pain are independent of uptake‑2 吗啡对条件性场所偏好和疼痛的影响与摄取-2 无关

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-14 DOI: 10.55782/ane-2024-2517

Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh

{"title":"Effects of morphine on conditioned place preference and pain are independent of uptake‑2","authors":"Mohammad Saeid Souri, M. Alavi, Ali Ahmadian Salami, A. Roohbakhsh","doi":"10.55782/ane-2024-2517","DOIUrl":"https://doi.org/10.55782/ane-2024-2517","url":null,"abstract":"Morphine changes neurotransmitter release, including norepinephrine, dopamine, and serotonin. Decynium‑22 (D22) inhibits an alternative neurotransmitter removal pathway, namely uptake‑2. Uptake‑2 includes plasma membrane monoamine transporter (PMAT) and organic cation transporters that have a low affinity, but high capacity for uptake of various monoamines such as norepinephrine, dopamine, and serotonin. This study was done to assess the effect of uptake‑2 inhibition on morphine‑induced conditioned place preference (CPP) and analgesia. In this study, the effects of morphine and/or D22 on CPP were evaluated following intraperitoneal injection in mice. Afterward, changes in motor activity were evaluated by the open field test. Using the tail‑flick model, the effects of D22 and/or morphine were evaluated on the pain threshold. The results showed that 20 mg/kg of morphine induced a place preference response. D22, at the dose of 0.03 mg/kg, caused place avoidance, while at the dose of 0.3 mg/kg, it produced a notable place preference response. Co‑administration of D22 and morphine showed that morphine reversed the CPP aversion induced by D22 at the lowest dose. Motor activity did not alter. In the tail‑flick test, morphine, at the dose of 3 mg/kg but not 1 mg/kg, increased the pain threshold. D22 induced significant analgesic responses. Co‑administration of D22 and morphine caused considerable analgesic effects. The findings revealed that D22 induced both conditioned aversion and preference depending on the dose while morphine induced CPP. Both drugs produced analgesia.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"608 ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139839087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0