Acta neurobiologiae experimentalis最新文献_第3页

Dissociable neuronal mechanism for different crossmodal correspondence effects in humans 人类不同跨模态对应效应的可分离神经元机制

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-05-21 DOI: 10.55782/ane-2024-2439

C. Jaap, Michael Rose

{"title":"Dissociable neuronal mechanism for different crossmodal correspondence effects in humans","authors":"C. Jaap, Michael Rose","doi":"10.55782/ane-2024-2439","DOIUrl":"https://doi.org/10.55782/ane-2024-2439","url":null,"abstract":"Crossmodal correspondences (CMCs) refer to associations between seemingly arbitrary stimulus features in different sensory modalities. Pitch‑size correspondences refer to the strong association of e.g., small objects with high pitches. Pitch‑elevation correspondences refer to the strong association of e.g., visuospatial elevated objects with high pitches. We used functional magnetic resonance imaging (fMRI) to study the neural components, which underlie the CMCs in pitch‑size and spatial pitch‑elevation. This study focuses on answering the question of whether or not different CMCs are driven by similar neural mechanisms. The comparison of congruent against incongruent trials allows the estimation of CMC effects across different CMCs. The analysis of the measured neural activity in different CMCs strongly pointed toward different mechanisms which are involved in the processing of pitch‑size and pitch‑elevation correspondences. Differential, whole brain effects were observed within the superior parietal lobule (SPL), cerebellum and Heschls’ gyrus (HG). Further, the angular gyrus (AnG), the intraparietal sulcus (IPS) and anterior cingulate cortex (ACC) were engaged in processing the CMCs but showed different effects for processing congruent compared to incongruent stimulus presentations. Within pitch‑size significant effects in the AnG and ACC were found for congruent stimulus presentations whereas for pitch‑elevation, significant effects in the ACC and IPS were found for incongruent stimulus presentations. In summary, the present results indicated differential neural processing in different simple audio‑visual CMCs.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"85 4","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141116298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of professional dance training on characteristics of postural sway 专业舞蹈培训对姿势摇摆特征的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-05-21 DOI: 10.55782/ane-2024-2399

J. W. Błaszczyk, Artur Fredyk

{"title":"Impact of professional dance training on characteristics of postural sway","authors":"J. W. Błaszczyk, Artur Fredyk","doi":"10.55782/ane-2024-2399","DOIUrl":"https://doi.org/10.55782/ane-2024-2399","url":null,"abstract":"The stability of human upright posture determines the range and dynamics of movements performed. Consequently, the repertoire and quality of the movements performed by a dancer are mainly determined by the efficiency of postural control. This is of particular importance in professional dance training that should focus on shaping optimal movement‑posture interaction. To get a deeper insight into this problem, the impact of the training on postural sway characteristics during quiet stance was analyzed in 16 female students in the seventh grade of a ballet school and compared with the size‑ and age‑matched group of secondary school students. Center of pressure trajectories were recorded for 25.6 s while standing quiet with eyes open (EO) and then with eyes closed (EC). The assessment of postural control was based on novel normalized sway parameters including sway vector (SV), sway anteroposterior (AP) and mediolateral (ML) directional indices (DIAP and DIML), and sway ratios (SRAP and SRML). The results document a significant contribution of vision to postural stability control in ballet students, which seems to compensate for training‑related changes in joint mobility and altered activity ranges of the legs’ muscles. In the control group standing with EC, SV amplitude increased only by 18% whereas in the ballet students tested in the same conditions, the increase exceeded 72%. Under full control of standing posture (EO test), the training‑related increase of leg muscle forces allows dancers to maintain balance with lesser effort as documented here by 21% reduced SRAP. Additionally, the dancers while tested with their EC exhibited a 12% increase in the anteroposterior sway with a concomitant reduction of the mediolateral sway. The resulting changes in the postural control asymmetry were documented by both DIAP/DIML and SV azimuth. In conclusion, our novel analysis of postural sway seems a useful tool in monitoring the effects of trainingas well as the proper course of postural control development in children and adolescents.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"11 6","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141114436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcranial direct current stimulation in the treatment of alcohol, tobacco and opioid use disorder in clinical studies. 经颅直流电刺激治疗酒精、烟草和阿片类药物使用障碍的临床研究。

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-05-16 DOI: 10.55782/ane-2024-2479

Begüm Merve Çabuk, Zozan Guleken

{"title":"Transcranial direct current stimulation in the treatment of alcohol, tobacco and opioid use disorder in clinical studies.","authors":"Begüm Merve Çabuk, Zozan Guleken","doi":"10.55782/ane-2024-2479","DOIUrl":"https://doi.org/10.55782/ane-2024-2479","url":null,"abstract":"<p><p>Transcranial direct current stimulation (tDCS) is a promising research tool to address substance abuse, including alcohol, tobacco, opioid, and drug use disorders. The present literature review compared previous studies conducted with various current intensities, application regions, durations of stimulations, and different region targets of the brain. Studies based on the analyses conducted after tDCS administration in substance use disorder were promising for the use of tDCS as adjunctive therapy to reduce visible psychological and neurological symptoms of the addiction. Therefore, we aimed to provide an insight into the current state of research on tDCS as a therapeutic intervention in substance use disorders, identify gaps in the literature, and emphasize future investigation areas. Ultimately, the review sought to contribute to the understanding of the role of tDCS in addressing the complex challenges posed by substance use disorder, and its potential as a complementary or adjunctive treatment modality in addiction care. The present study identified that the left dlPFC and brain regions were effective targets for 1 mA and 2 mA tDCS current density in tobacco/nicotine use disorder. Also, the left dlPFC and 2 mA current density were identified as effective targets for tDCS in alcohol use disorder. Furthermore, left dlPFC and 2 mA current density were identified as effective targets for tDCS in opioid use disorder. Additionally, the right/left dlPFC, orbital frontal cortex, thalamus, and 2 mA current density were identified as effective targets for tDCS in other drug or substance use disorders. Animal studies demonstrated that tDCS was promising in reducing neuropathic pain, modulating neuropeptide Y activity, and reducing the redevelopment of ethanol consumption in animal models. However, further research is required to fully understand the optimal tDCS application parameters.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 2","pages":"111-127"},"PeriodicalIF":1.4,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141858745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel insight into the neuroprotective effects ofcannabidiol: maintained apelin/dopamine synthesis, NRF2 signaling, and AKT/CREB/BDNF gene expressions 对大麻二酚神经保护作用的新认识：维持芹菜素/多巴胺合成、NRF2信号传导和AKT/CREB/BDNF基因表达

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-26 DOI: 10.55782/ane-2024-2546

Melike DOĞAN ÜNLÜ, S. Asci, O. Imeci, Adem Milletsever, Özlem Özmen, S. Sezer, Serpil Demirci

{"title":"A novel insight into the neuroprotective effects ofcannabidiol: maintained apelin/dopamine synthesis, NRF2 signaling, and AKT/CREB/BDNF gene expressions","authors":"Melike DOĞAN ÜNLÜ, S. Asci, O. Imeci, Adem Milletsever, Özlem Özmen, S. Sezer, Serpil Demirci","doi":"10.55782/ane-2024-2546","DOIUrl":"https://doi.org/10.55782/ane-2024-2546","url":null,"abstract":"Neuroinflammation is a process associated with degeneration and loss of neurons in different parts of the brain. The most important damage mechanisms in its formation are oxidative stress and inflammation. This study aimed to investigate the protective effects of cannabidiol (CBD) against neuroinflammation through various mechanisms. Thirty‑two female rats were randomly divided into 4 groups as control, lipopolysaccharide (LPS), LPS + CBD and CBD groups. After six hours following LPS administration, rats were sacrificed, brain and cerebellum tissues were obtained. Tissues were stained with hematoxylin‑eosin for histopathological analysis. Apelin and tyrosine hydroxylase synthesis were determined immunohistochemically. Total oxidant status and total antioxidant status levels were measured, and an oxidative stress index was calculated. Protein kinase B (AKT), brain-derived neurotrophic factor (BDNF), cyclic‑AMP response element‑binding protein (CREB) and nuclear factor erythroid 2‑related factor 2 (NRF2) mRNA expression levels were also determined. In the LPS group, hyperemia, degeneration, loss of neurons and gliosis were seen in all three tissues. Additionally, Purkinje cell loss in the cerebellum, as well as neuronal loss in the cerebral cortex and hippocampus, were found throughout the LPS group. The expressions of AKT, BDNF, CREB and NRF2, apelin and tyrosine hydroxylase synthesis all decreased significantly. CBD treatment reversed these changes and ameliorated oxidative stress parameters. CBD showed protective effects against neuroinflammation via regulating AKT, CREB, BDNF expressions, NRF2 signaling, apelin and tyrosine hydroxylase synthesis.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"8 7","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140378719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of morphine administration on GluN3B NMDA receptor subunit mRNA expression in rat brain 吗啡对大鼠脑内 GluN3B NMDA 受体亚基 mRNA 表达的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-26 DOI: 10.55782/ane-2024-2545

Shahrzad Nazari, Mitra-Sadat Sadat-Shirazi, Ali Shahbazi, K. Ghaffari, N. Vousooghi

{"title":"The effect of morphine administration on GluN3B NMDA receptor subunit mRNA expression in rat brain","authors":"Shahrzad Nazari, Mitra-Sadat Sadat-Shirazi, Ali Shahbazi, K. Ghaffari, N. Vousooghi","doi":"10.55782/ane-2024-2545","DOIUrl":"https://doi.org/10.55782/ane-2024-2545","url":null,"abstract":"Opioid addiction is critically dependent on the activation of N‑methyl‑D‑aspartate (NMDA) receptors, which are widely found in the mesocorticolimbic system. Meanwhile, opioid addiction may affect the expression level of NMDA receptor subunits. The existence of GluN3 subunits in the NMDA receptor’s tetramer structure reduces the excitatory current of the receptor channel. We evaluated the changes in the mRNA expression pattern of the GluN3B subunit of the NMDA receptor in rat brains following acute and chronic exposure to morphine. Chronic, escalating intraperitoneal doses of morphine or saline were administered twice daily to male Wistar rats for six days. Two other groups were injected with a single acute dose of morphine or saline. The mRNA level of the GluN3B subunit of the NMDA receptor in the striatum, hippocampus, and nucleus accumbens (NAc) was measured by real‑time PCR. mRNA expression of the GluN3B subunit was considerably augmented (3.15 fold) in the NAc of animals chronically treated with morphine compared to the control group. The difference between rats that were chronically administered morphine and control rats was not statistically significant for other evaluated brain areas. In rats acutely treated with morphine, no significant differences were found for GluN3B subunit expression in the examined brain regions compared to the control group. It was concluded that chronic exposure to morphine notably increased the GluN3B subunit of the NMDA receptor in NAc. The extent of the impact of this finding on opioid addiction and its features requires further evaluation in future studies.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"101 2","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of neurogranin levels in a rat model of diffuse axonal injury 评估弥漫性轴突损伤大鼠模型中的神经粒蛋白水平

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-25 DOI: 10.55782/ane-2024-2543

Ozan Başkurt, Seckin Aydin, I. Avci, Pinar Ozay Nayir, M. V. Aydin

{"title":"Evaluation of neurogranin levels in a rat model of diffuse axonal injury","authors":"Ozan Başkurt, Seckin Aydin, I. Avci, Pinar Ozay Nayir, M. V. Aydin","doi":"10.55782/ane-2024-2543","DOIUrl":"https://doi.org/10.55782/ane-2024-2543","url":null,"abstract":"Diffuse axonal injury (DAI), one of the most common and devastating type of traumatic brain injury, is the result of the shear force on axons due to severe rotational acceleration and deceleration. Neurogranin (NRGN) is a postsynaptic protein secreted by excitatory neurons, and synaptic dysfunction can alter extracellular NRGN levels. In this study, we examined NRGN levels in serum and cerebrospinal fluid (CSF) after experimental DAI in terms of their diagnostic value. Experimental DAI was induced using the Marmarou technique in male Wistar albino rats. Serum and CSF NRGN levels of the sham group, one‑hour, six‑hour, 24‑hour, and 72‑hour post‑DAI groups were measured by ELISA method. DAI was verified by staining with hematoxylin‑eosin and β‑amyloid precursor protein in the rat brain samples. While no histopathological and immunohistochemical changes were observed in the early hours of the post‑DAI groups, the staining of the β‑APP visibly increased over time, with positivity being most frequent and intense in the 72‑hour group. It was found that serum NRGN levels were significantly lower in the 6‑hour group than in the sham group. The serum NRGN levels in the 24‑hour group were significantly higher than those in the sham group. This study showed a dichotomy of post‑DAI serum NRGN levels in consecutive time periods. NRGN levels in CSF were higher in the one‑hour group than in the sham group and returned to baseline by 72 hours, although not significantly. Our study provides an impression of serum and CSF NRGN levels in a rat DAI model in consecutive time periods. Further studies are needed to understand the diagnostic value of NRGN.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" December","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140383196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time‑dependent changes in blood cells, NIHSS and mRS according to reperfusion treatment type in stroke patients who developed hemorrhagic complication 发生出血性并发症的脑卒中患者的血细胞、NIHSS 和 mRS 随时间变化的再灌注治疗类型

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-22 DOI: 10.55782/ane-2024-2540

H. Kömürcü, E. Gözke, Irmak Salt, Isil Kalyoncu Aslan, Pelin Doğan Ak

{"title":"Time‑dependent changes in blood cells, NIHSS and mRS according to reperfusion treatment type in stroke patients who developed hemorrhagic complication","authors":"H. Kömürcü, E. Gözke, Irmak Salt, Isil Kalyoncu Aslan, Pelin Doğan Ak","doi":"10.55782/ane-2024-2540","DOIUrl":"https://doi.org/10.55782/ane-2024-2540","url":null,"abstract":"Hemorrhagic complications may be seen following reperfusion therapy with rtPA and/or thrombectomy after acute ischemic stroke (AIS). Neutrophils, lymphocytes, and platelets have important roles in the inflammatory and immune responses that develop in these patients. We investigated time‑dependent changes in blood cells, NIHSS and mRS values according to type of reperfusion therapy in AIS patients who developed cerebral hemorrhage. In AIS patients who underwent rtPA and/or thrombectomy and developed cerebral hemorrhage within the first 24 hours after treatment, leukocyte, neutrophil, lymphocyte, platelet counts and their ratios were recorded on admission, 1st, 3rd, and 7th days. NIHSS values on admission, 3rd days and mRS values on admission, discharge, and the 3rd month were recorded. These values were compared according to the type of reperfusion therapy. Out of 436 AIS patients, rtPA was applied in 50.5%, thrombectomy in 28.2%, and rtPA+thrombectomy in 21.3%. Hemorrhage developed in 25.5% of the patients. Patients treated with thrombectomy had a greater rate of cerebral hemorrhage. Pre‑stroke mRS values were lower in all therapy types than mRS scores at discharge and the 3rd month. The NIHSS scores did not differ significantly in 3 days. Depending on the type of reperfusion treatment, there are a few time‑dependent significant changes observed in the blood cell counts and ratios. In conclusion, there is a relation between the type of reperfusion therapy and the time‑dependent changes in blood cells and ratios as well as mRS scores among AIS patients who have undergone rtPA and/or thrombectomy and developed cerebral hemorrhage.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 11","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140387185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preventive effects of crocin, a natural compound from saffron, against nicotine‑induced oxidative stress and neurobehavioral disturbances 藏红花中的天然化合物黄花苷对尼古丁诱导的氧化应激和神经行为紊乱的预防作用

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-19 DOI: 10.55782/ane-2024-2538

M. Motaghinejad, M. Motevalian, Antoni Sureda

{"title":"Preventive effects of crocin, a natural compound from saffron, against nicotine‑induced oxidative stress and neurobehavioral disturbances","authors":"M. Motaghinejad, M. Motevalian, Antoni Sureda","doi":"10.55782/ane-2024-2538","DOIUrl":"https://doi.org/10.55782/ane-2024-2538","url":null,"abstract":"Nicotine is a psychostimulant that induces neurochemical and behavioral changes upon chronic administration, leading to neurodegenerative conditions associated with smoking. As of now, no preventive or therapeutic strategies are known to counteract nicotine‑induced neurodegeneration. In this study, we explore the neuroprotective effects of crocin, a bioactive agent commonly found in saffron – a spice derived from the flower of Crocus sativus – using a rat model. The dose‑dependent effects of crocin were evaluated in nicotine‑induced neurodegeneration and compared with a control group. Neurobehavioral changes, assessed through the elevated plus maze, the open field test, the forced swim test, and the Morris water maze, as well as oxidative stress in the hippocampus, were evaluated. Interestingly, nicotine administration resulted in depression, anxiety, and abnormal motor and cognitive functions, while crocin treatment protected the rat brain from these abnormalities. The beneficial effects of crocin were associated with reduced oxidative stress biomarkers such as malondialdehyde, along with increases in superoxide dismutase, glutathione peroxidase, and glutathione reductase activities. These results demonstrate that crocin can mitigate nicotine‑induced neurodegeneration by reducing oxidative stress, potentially offering a protective measure against neurodegenerative effects in smokers.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 7","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model 左乙拉西坦对颞叶癫痫模型中 DNA 氧化和谷胱甘肽含量的影响

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-03-19 DOI: 10.55782/ane-2024-2537

Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez

{"title":"Effect of levetiracetam on DNA oxidation and glutathione content in a temporal lobe epilepsy model","authors":"Itzel Jatziri Contreras-García, C. Bandala, Iván Ignacio-Mejía, L. A. Pichardo-Macías, J. Mendoza-Torreblanca, M. E. García-Cruz, Saúl Gómez‑Manzo, N. Cárdenas-Rodríguez","doi":"10.55782/ane-2024-2537","DOIUrl":"https://doi.org/10.55782/ane-2024-2537","url":null,"abstract":"Levetiracetam (LEV) is a drug commonly used as an anticonvulsant. However, recent evidence points to a possible role as an antioxidant. We previously demonstrated the antioxidant properties of LEV by significantly increasing catalase and superoxide dismutase activities and decreasing the hydrogen peroxide (H2O2) levels in the hippocampus of rats with temporal lobe epilepsy (TLE) showing scavenging properties against the hydroxyl radical. The aim of the present work was to evaluate, the effect of LEV on DNA oxidation, by determining 8‑hydroxy‑2‑deoxyguanosine (8‑OHdG) levels, and glutathione content, through reduced (GSH) and oxidized (GSSG) glutathione levels, in the hippocampus of rats with TLE. Male Wistar rats were assigned to the control (CTRL), CTRL+LEV, epileptic (EPI) and EPI+LEV groups. TLE was induced using the lithium‑pilocarpine model. Thirteen weeks after TLE induction, LEV was administered for one week through osmotic pumps implanted subcutaneously. The determination of 8‑OHdG, GSH and GSSG levels were measured using spectrophotometric methods. We showed that LEV alone significantly increased 8‑OHdG and GSSG levels in the hippocampus of control rats compared to those in epileptic condition. No significant differences in GSH levels were observed. LEV could induce changes in the hippocampus increasing DNA oxidation and GSSG levels under nonepileptic condition but not protecting against the mitochondrial dysfunction observed in TLE probably by mechanisms related to changes in chromatin structure, neuroinflammation and alterations in redox components.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":" 2","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse 将本征信号光学成像作为描述幼鼠初级视觉皮层定向敏感性的工具

IF 1.4 4区医学

Acta neurobiologiae experimentalis Pub Date : 2024-02-29 DOI: 10.55782/ane-2024-2397

I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel

{"title":"Optical imaging of the intrinsic signal as a tool tocharacterize orientation sensitivity in the primaryvisual cortex of the young mouse","authors":"I. Raciborska, Piotr Dzwiniel, Katarzyna Kordecka, A. Posłuszny, W. Waleszczyk, Andrzej Wróbel","doi":"10.55782/ane-2024-2397","DOIUrl":"https://doi.org/10.55782/ane-2024-2397","url":null,"abstract":"We employed intrinsic signal optical imaging (ISOI) to investigate orientation sensitivity bias in the visual cortex of young mice. Optical signals were recorded in response to the moving light gratings stimulating ipsi‑, contra‑ and binocular eye inputs. ISOI allowed visualization of cortical areas activated by gratings of specific orientation and temporal changes of light scatter during visual stimulation. These results confirmed ISOI as a reliable technique for imaging the activity of large populations of neurons in the mouse visual cortex. Our results revealed that the contralateral ocular input activated a larger area of the primary visual cortex than the ipsilateral input, and caused the highest response amplitudes of light scatter signals to all ocular inputs. Horizontal gratings moved in vertical orientation induced the most significant changes in light scatter when presented contralaterally and binocularly, surpassing stimulations by vertical or oblique gratings. These observations suggest dedicated integration mechanisms for the combined inputs from both eyes. We also explored the relationship between point luminance change (PLC) of grating stimuli and ISOI time courses under various orientations of movements of the gratings and ocular inputs, finding higher cross-correlation values for cardinal orientations and ipsilateral inputs. These findings suggested specific activation of different neuronal assemblies within the mouse’s primary visual cortex by grating stimuli of the corresponding orientation. However, further investigations are needed to examine this summation hypothesis. Our study highlights the potential of optical imaging as a valuable tool for exploring functional‑anatomical relationships in the mouse visual system.","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"15 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140415917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0