Acta neurobiologiae experimentalis最新文献

{"title":"Hydroxycinnamates alleviate chronic unpredictable mild stress‑induced depressive‑like behavior and neuroinflammation in mice.","authors":"Manas Kinra, Niraja Ranadive, Madhavan Nampoothiri, Jayesh Mudgal, Devinder Arora","doi":"10.55782/ane-2024-2681","DOIUrl":"https://doi.org/10.55782/ane-2024-2681","url":null,"abstract":"<p><p>The polyphenolic compounds ferulic acid (FA) and p‑coumaric acid (PCA) have been extensively studied for their free radical scavenging and anti‑inflammatory properties. Both compounds are present in food and beverages commonly consumed globally. Our molecular modeling, in‑vitro, and in‑vivo studies suggest that the compounds may be neuroprotective by modulating the nucleotide‑binding domain, leucine‑rich‑containing family, pyrin domain‑containing‑3 (NLRP3) inflammasome pathway. The current study explored the dose‑dependent neuroprotective potential of FA and PCA in a chronic unpredictable mild stress (CUMS) mouse model. Male Swiss albino mice were divided into nine groups consisting of control (CON), CUMS, FA10 (10 mg/kg FA), FA40 (40 mg/kg FA), FA160 (160 mg/kg FA), PCA10 (10 mg/kg PCA), PCA40 (40 mg/kg PCA), PCA160 (160 mg/kg PCA), and FLX (10 mg/kg fluoxetine). All animals, except the CON group, received random mild stressors for 21 days, and from day 22‑42, the treatments were administered alongside the stressors. Behavioral assessments were performed on day 42, followed by sample collection. Brain homogenates from CUMS‑exposed animals expressed elevated levels of the pro‑inflammatory cytokines interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑alpha (TNF‑α), and oxidative stress markers. Treatment with FA and PCA effectively reduced cytokine release and oxidative stress, alleviating the depressive‑like behavior.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"85 1","pages":"58-66"},"PeriodicalIF":1.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulation of miR‑24‑3p and miR‑186‑5p and GABA_A receptor expression in focal cortical dysplasia.","authors":"Arpna Srivastava, Yogesh Agarwal, Soumil Dey, Ramesh Doddamani, Mehar Chand Sharma, Manjari Tripathi, Poodipedi Sarat Chandra, Sanjeev Lalwani, Aparna Banerjee Dixit, Jyotirmoy Banerjee","doi":"10.55782/ane-2024-2656","DOIUrl":"https://doi.org/10.55782/ane-2024-2656","url":null,"abstract":"<p><p>Spontaneous synaptic activity mediated by GABAA receptor is associated with epileptogenicity in focal cortical dysplasia (FCD). miRNAs are potentially involved in the regulation of GABAA receptor subunit expression and activity. This study aimed to determine the expression of miRNAs in FCD and correlate their expression level with mRNA levels of GABAA receptor subunits. Expression of GABAA receptor subunits (α1 and α4) and miRNAs (miR‑155‑5p, miR‑186‑5p, and miR‑24‑3p) were evaluated using real‑time PCR in resected brain samples from FCD patients. miRNA levels were also determined in the serum of FCD patients. Spontaneous GABAA receptor‑mediated synaptic activity was measured using patch clamp technique. Significant increase in α1 and α4 subunit expression and miR‑155‑5p levels, while decrease in miR‑24‑3p and miR‑186‑5p levels, was observed in the brain samples of FCD. In the serum of FCD patients, miR‑155‑5p levels were increased, whereas miR‑24‑3p and miR‑186‑5p levels remained unaltered. Increased α4 subunit expression in FCD might be due to reduced levels of miR‑24‑3p and miR‑186‑5p. In addition, reduced miR‑186‑5p levels might be responsible for increased expression of α1 subunit. We also observed an increase in the spontaneous GABAA receptor‑mediated synaptic transmission in FCD. In conclusion, dysregulation of miRNAs and GABAA receptor expression suggest that these miRNAs may contribute to altered GABAA receptor‑mediated synaptic activity in FCD.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"85 1","pages":"16-28"},"PeriodicalIF":1.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The phenomenon of sensory processing: historical overview, theoretical models, and neurophysiological underpinnings.","authors":"Natalia Grobelna, Radosław Rutkowski, Filip Rybakowski, Janusz Rybakowski, Ewa Ferensztajn-Rochowiak","doi":"10.55782/ane-2024-2556","DOIUrl":"10.55782/ane-2024-2556","url":null,"abstract":"<p><p>The article provides a review of the sensory processing (SP) phenomenon, its origins, theoretical models, and neurophysiological foundations. Initiated by A. Jean Ayres' research on sensory integration in the 1960s and 70s, this field has evolved, leading to the development of concepts such as Winnie Dunn's four quadrant model and Miller's ecological model of sensory modulation. Over the years, based on theoretical considerations, the concepts of sensory processing disorder and sensory processing sensitivity were formulated. The article highlights the role of temperament and its impact on sensory processing, suggesting that individual differences can significantly affect how people respond to sensory stimuli. The neurophysiological basis including sensory gating, electrodermal responses, and neuroimaging methods is presented. There has been an interest in the relationship between SP and mental disorders in adults, despite the lack of a formal diagnosis in DSM‑5 and ICD classifications. The literature analysis reveals the complexity of the subject, indicating the need for further research in this field.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"359-370"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperine relieves neuropathic pain induced by paclitaxel in mice.","authors":"Toktam Sahranavard, Vahideh Ghorani, Fatemeh Forouzanfar, Samaneh Sadat Asadi Kakhki, Ebrahim Nikfar Dastaki, Ebrahim Golmakani, Ramin Rezaee","doi":"10.55782/ane-2024-2590","DOIUrl":"10.55782/ane-2024-2590","url":null,"abstract":"<p><p>Piperine is an amide alkaloid isolated from the black pepper plant. This study examined the pain‑relieving activity of piperine against paclitaxel (PTX)‑induced neuropathy. Male mice were divided into 6 groups: Sham‑operated group (remained intact), PTX group (PTX‑treated mice receiving normal saline), PTX+ piperine 10, 25, and 50 mg/kg groups (PTX‑treated mice receiving piperine) and positive control group (PTX‑treated mice receiving imipramine 10 mg/kg). Neuropathic pain was induced by PTX 2 mg/kg/day on days 1, 3, 5 and 7. On day 7, behavioral tests were conducted and serum levels of interleukin‑6 (IL‑6), tumor necrosis factor‑alpha (TNF‑α), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were assayed. PTX produced significant thermal hyperalgesia compared to the sham group. Piperine at all doses alleviated neuropathic pain, and significantly decreased IL‑6, TNF‑α, and MDA, but induced CAT and SOD activities compared to the control group. Piperine could confer beneficial effects against neuropathic pain, at least partially, via reduction of inflammatory and oxidative stress markers.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"332-340"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leflunomide exerts neuroprotective effects in an MPTP‑treated mouse model of Parkinsonism.","authors":"Yeşim Civil Ürkmez, Seda Kirmizikan, Caner Günaydin, Esra Cikler, Sirri Bilge, Bahattin Avci, Sebati Sinan Ürkmez","doi":"10.55782/ane-2024-2579","DOIUrl":"10.55782/ane-2024-2579","url":null,"abstract":"<p><p>Neuroinflammation and the immune response are recognized as significant mechanisms contributing to the progression and pathophysiology of Parkinson's disease (PD). Consequently, extensive research is being conducted on drugs targeting inflammation and immune response. Leflunomide, known for its anti‑inflammatory and immunomodulatory properties, is currently used as a disease‑modifying agent for the treatment of rheumatoid arthritis. The objective of this study was to investigate the effect of leflunomide on PD. The PD model was established by administering 18 mg/kg of 1‑methyl‑4‑phenyl‑1,2,3,6‑tetrahydropyridine (MPTP) intraperitoneally for 5 consecutive days. Leflunomide was administered intraperitoneally at doses of 1, 5, and 10 mg/kg for 14 days. Motor and behavioral deficits were assessed using the rotarod test, locomotor activity assessment, hanging wire test, and pole test. MPTP administration impaired motor function and locomotor activity, and caused muscle weakness and bradykinesia. Leflunomide at a dose of 10 mg/kg mitigated the severity of motor deficits and muscle weakness. Furthermore, leflunomide at a dose of 10 mg/kg suppressed the MPTP‑induced elevation of interleukin‑2, interleukin‑6, and tumor necrosis factor‑alpha levels in the brain tissue. Similarly, leflunomide attenuated the increased expression of nuclear factor kappa B and inducible nitric oxide synthase caused by MPTP treatment. Moreover, leflunomide at a dose of 10 mg/kg preserved neuronal integrity and prevented the loss of tyrosine hydroxylase expression induced by MPTP administration. Based on our findings, leflunomide exhibited a beneficial effect on the MPTP‑induced PD model, potentially through modulation of anti‑inflammatory mechanisms.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"319-331"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The integral role of <i>PTEN</i> in brain function: from neurogenesis to synaptic plasticity and social behavior.","authors":"Natalia Chwin, Anna Kiryk","doi":"10.55782/ane-2024-2657","DOIUrl":"10.55782/ane-2024-2657","url":null,"abstract":"<p><p>The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) gene is a critical tumor suppressor that plays an essential role in the development and functionality of the central nervous system. Located on chromosome 10 in humans and chromosome 19 in mice, PTEN encodes a protein that regulates cellular processes such as division, proliferation, growth, and survival by antagonizing the PI3K‑Akt‑mTOR signaling pathway. In neurons, PTEN dephosphorylates phosphatidylinositol‑3,4,5‑trisphosphate (PIP3) to PIP2, thereby modulating key signaling cascades involved in neurogenesis, neuronal migration, and synaptic plasticity. PTEN is crucial for embryonic neurogenesis, controlling the proliferation of neural progenitor cells and guiding the migration and proper lamination of neurons in cortical and hippocampal structures. It also regulates dendritic growth and axon guidance, ensuring correct neuronal connectivity. In postnatal neurogenesis, PTEN maintains the balance of stem cell proliferation and integration of new neurons into existing circuits, particularly in the hippocampal dentate gyrus. Animal models with PTEN deletion or mutation exhibit significant structural and functional neuronal abnormalities, including enlarged soma and dendritic hypertrophy, increased synaptic density, and altered synaptic plasticity mechanisms such as long‑term potentiation and long‑term depression. These changes lead to deficits in learning and memory tasks, as well as impairments in social behaviors. PTEN mutations are associated with neurodevelopmental disorders like intellectual disability, epilepsy, and autism spectrum disorders accompanied by macrocephaly. Understanding PTEN's mechanisms offers valuable insights into its contributions to neurodevelopmental disorders and presents potential therapeutic targets for cognitive impairments and neurodegenerative diseases. Future research should focus on elucidating PTEN's functions in mature neurons and its influence on established neuronal networks, which may have significant implications for memory enhancement and behavioral modifications.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"309-318"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different faces of autism: Patients with mutations in <i>PTEN</i> and <i>FMR1</i> genes.","authors":"Adam Gorlewicz, Ewelina Kanpska","doi":"10.55782/ane-2024-2664","DOIUrl":"10.55782/ane-2024-2664","url":null,"abstract":"<p><p>Autism spectrum disorder (ASD) is among the most common neurodevelopmental conditions in humans. While public awareness of the challenges faced by individuals with autism is steadily increasing, the underlying causes of abnormalities observed in ASD remains incompletely understood. The autism spectrum is notably broad, with symptoms that can manifest in various forms and degrees of severity. Core features of ASD, such as communication difficulties, impaired social interactions, and restricted patterns of behavior, interests, and activities, are often accompanied by other co‑occurring conditions, such as anxiety. ASD affects individuals regardless of gender, race, or ethnicity. Although we are currently unable to pinpoint a single definitive cause of autism, it is clear that genetics play a crucial role in its development. The first genes associated with an increased risk for ASD were discovered in rare monogenic disorders, such as fragile X syndrome (FXS), caused by mutations in the fragile X messenger ribonucleoprotein 1 (FMR1) gene, and macrocephaly, linked to mutations in the phosphatase and tensin homolog (PTEN) gene. This review aims to summarize the current knowledge of ASD in patients with mutations in the FMR1 and PTEN genes.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"352-358"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the neurobiological mechanisms of LPS‑induced memory impairment.","authors":"Ahmad Golkar, Mohammad Dalfardi, Mahdiyeh Hedayati-Moghadam, Hedyeh Askarpour, Mahmoud Hosseini, Yousef Baghcheghi","doi":"10.55782/ane-2024-2629","DOIUrl":"10.55782/ane-2024-2629","url":null,"abstract":"<p><p>In recent years, growing evidence suggests that lipopolysaccharide (LPS), a bacterial endotoxin found in the outer membrane of gram‑negative bacteria, can influence cognitive functions, particularly memory formation and retrieval. However, the underlying mechanisms through which LPS exerts its effects on memory remain incompletely understood. This review used various electronic databases, including PubMed, Scopus, and Web of Science, to identify relevant studies published between 2000 and 2024. Articles were selected based on their focus on LPS‑induced memory impairments, including experimental models, molecular pathways, and neurochemical alterations. LPS administration has been consistently shown to disrupt memory processes in both animals and humans, although the magnitude and duration of memory impairments might vary depending on factors such as dose, timing, and context of LPS exposure. Several potential mechanisms have been proposed to explain LPS‑induced memory deficits, including neuroinflammation, alterations in synaptic plasticity, disruption of neurotransmitter systems, and dysfunction of the blood‑brain barrier. Moreover, LPS has been found to activate immune signaling pathways, such as toll‑like receptors, interleukins, and microglia, which can further contribute to cognitive impairments. Such insights may pave the way for the development of targeted therapeutic interventions aimed at ameliorating memory deficits associated with conditions involving LPS exposure, including bacterial infections, sepsis, and neuroinflammatory disorders.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"371-394"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of miRNA to treatment with <i>Hypericum perforatum</i> L. oil in multiple sclerosis.","authors":"Huri Dedeakayogullari, Zozan Guleken, Sahabettin Selek, Nur Dogan, Busra Yuce, Emine Seyda Teloglu, Gulreyhan Sonuc, Cagla Yildiz, Esra Tiftik, Aysu Kilic, Beren Yildizbas, Zeynep Ece Bulut","doi":"10.55782/ane-2024-2571","DOIUrl":"10.55782/ane-2024-2571","url":null,"abstract":"<p><p>MicroRNA‑regulated gene expression plays an important role in autoimmune diseases, such as multiple sclerosis (MS). This study investigated the expression patterns of microRNAs (miRNAs) in MS in brain tissues using an animal experimental autoimmune encephalomyelitis (EAE) model treated with Hypericum perforatum (HP) oil. C57BL/6 J mice were divided into two groups: MS and control. The MS group was subdivided into sham (MS) and MS+HP. After the EAE induction treatment protocol, the patterns of miRNA expression profiles were determined in brain samples of the groups. The array data identified eleven miRNAs and candidate miRNA validation was performed by RT‑qPCR. A literature review of the validated miRNAs found that six of the eleven miRNAs (miR‑200a‑3p, miR‑200b‑3p, miR‑200c‑3p, miR‑182‑5p, miR‑183‑5p, and miR‑1298‑5p) were directly associated with MS. These miRNAs have been suggested as biomarkers of MS because they are highly correlated with the pathology of the disease. Furthermore, miRNA array analysis identified five candidate miRNAs (miR‑299a‑5p, miR‑206‑3p, miR‑325‑5p, miR‑10b‑5p, miR‑429‑3p) that are highly likely to be associated with MS pathogenesis, which could be helpful in the diagnosis and treatment of MS disease. This research offers vital insights that could be utilized in creating biomarkers and advancing treatments for MS.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 4","pages":"341-351"},"PeriodicalIF":1.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long‑term effects of vitexin against development of pentylenetetrazole‑induced kindling in rats.","authors":"Denise Dias De Oliveira, Cassio Prinholato da Silva, Luiz Luciano Falconi-Sobrinho, Rene Oliveira Beleboni","doi":"10.55782/ane-2024-2575","DOIUrl":"https://doi.org/10.55782/ane-2024-2575","url":null,"abstract":"<p><p>Evidence is provided that the glycosylated flavonoid vitexin (apigenin‑8‑C‑beta‑D‑glucopyranoside) attenuates pentylenetetrazole (PTZ)‑induced acute tonic‑clonic seizures in rats. However, the effects of chronic and systemic vitexin in PTZ‑kindled rats remain unknown. The aim of this work was to investigate the effect of long‑term treatment with vitexin in the PTZ‑kindling model of epilepsy. Male Wistar rats received intraperitoneal injections of PTZ at a subconvulsive dose of 35 mg/kg every other day for 29 days. Either saline containing dimethyl sulfoxide - DMSO 1%  (vehicle), diazepam (2 mg/kg; positive control) or vitexin (2.5 mg/kg) was administered intraperitoneally 30 min before each PTZ injection. The behavioral reactions were recorded by 30 min immediately after each PTZ injection. Furthermore, on the 31st day, that is, 48 h after the latter dose of PTZ, the animals were euthanized and renal and hepatic biochemical markers were evaluated in blood serum. Chronic treatment with either diazepam or vitexin attenuated the seizures provoked by PTZ injections. Neither diazepam nor vitexin caused changes in renal levels of creatinine and urea and in hepatic levels of aspartate aminotransferase and alanine aminotransferase. Our findings suggest that chronic administration of vitexin attenuates the progression of PTZ‑induced kindling without causing side effects on kidneys and liver.</p>","PeriodicalId":7032,"journal":{"name":"Acta neurobiologiae experimentalis","volume":"84 3","pages":"266-274"},"PeriodicalIF":1.4,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}