Acta biochimica et biophysica Sinica最新文献_第7页

Melanoma-derived versican reactivates tumor-associated macrophages by upregulating pyruvate carboxylase through TLR2-MyD88-RelB axis under normoxia. 在正常缺氧条件下，黑色素瘤来源的versican通过TLR2-MyD88-RelB轴上调丙酮酸羧化酶来重新激活肿瘤相关的巨噬细胞。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-24 DOI: 10.3724/abbs.2025011

Yuxin Shu, Linmin Zhou, Jinqin Qian, Wei-Guo Zhu

引用次数: 0

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-23 DOI: 10.3724/abbs.2024236

Qian Chen, Huaying Zhang, Daokun Wang, Wenjing Liao, Yazhou Liu, Yurui Cai, Siyou Wang, Mengqian Yu

{"title":"mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein.","authors":"Qian Chen, Huaying Zhang, Daokun Wang, Wenjing Liao, Yazhou Liu, Yurui Cai, Siyou Wang, Mengqian Yu","doi":"10.3724/abbs.2024236","DOIUrl":"https://doi.org/10.3724/abbs.2024236","url":null,"abstract":"Mitochondrial dysfunction is implicated in numerous disorders, including type 2 diabetes, Alzheimer's disease and cancer. Long non-coding RNAs (lncRNAs) are emerging as pivotal regulators of cellular energy metabolism, yet their roles remain largely unclear. In this study, we identify an lncRNA named linc-PMB, which is associated with mTOR and promotes mitochondrial biogenesis, through microarray analysis. We demonstrate that the knockdown of linc-PMB results in significantly impaired mitochondrial respiration and biogenesis, along with altered expressions of related genes. Conversely, overexpression of linc-PMB markedly increases mitochondrial function. We further reveal that linc-PMB interacts with the RNA-binding protein HuR, promoting the stabilization of SIRT1 mRNA and a substantial increase in SIRT1 expression, which in turn activates the PGC-1α/mtTFA pathway and mitochondrial biogenesis. Collectively, our findings reveal a novel regulatory pathway in which linc-PMB, through its interaction with HuR, modulates the SIRT1/PGC-1α/mtTFA axis to maintain mitochondrial biogenesis and function.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Head-to-head: IL-21 triumphs over IL-15 in NK cell therapy for glioblastoma. 头对头：IL-21战胜IL-15在NK细胞治疗胶质母细胞瘤。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-23 DOI: 10.3724/abbs.2025009

Jake C Miller, Bihui Cao, Jia Shen

引用次数: 0

Daphnetin-mediated mitophagy alleviates intervertebral disc degeneration via the Nrf2/PINK1 pathway. 瑞香素介导的线粒体自噬通过Nrf2/PINK1途径缓解椎间盘退变。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-22 DOI: 10.3724/abbs.2025002

Yiting Tu, Jiaping Ren, Weiyuan Fang, Chencheng Zhou, Binli Zhao, Tianyong Hua, Yiqi Chen, Zhenya Chen, Yongzeng Feng, Haiming Jin, Xiangyang Wang

{"title":"Daphnetin-mediated mitophagy alleviates intervertebral disc degeneration via the Nrf2/PINK1 pathway.","authors":"Yiting Tu, Jiaping Ren, Weiyuan Fang, Chencheng Zhou, Binli Zhao, Tianyong Hua, Yiqi Chen, Zhenya Chen, Yongzeng Feng, Haiming Jin, Xiangyang Wang","doi":"10.3724/abbs.2025002","DOIUrl":"https://doi.org/10.3724/abbs.2025002","url":null,"abstract":"Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and effective therapies are still lacking. Reactive oxygen species (ROS) stress induces NLRP3 inflammasome activation, and this, along with extracellular matrix metabolism (ECM) degradation in nucleus pulposus cells (NPCs), plays a crucial role in the progression of IDD. Daphnetin (DAP) is a biologically active phytochemical extracted from plants of the Genus Daphne, which possesses various bioactivities, including antioxidant properties. In the present study, we demonstrate that DAP significantly attenuates tert-butyl hydroperoxide (TBHP)-induced ECM degradation, oxidative stress and NLRP3 inflammasome activation in NPCs. Furthermore, DAP could facilitate mitophagy to increase the removal of damaged mitochondria, consequently reducing mitochondrial ROS accumulation and alleviating NLRP3 inflammasome activation. Mechanistically, we unveil that DAP activates mitophagy by stimulating the Nrf2/PINK1 signaling pathway in TBHP-induced NPCs. In vivo experiments further corroborate the protective effect of DAP against IDD progression in a rat model induced by disc puncture. Accordingly, our findings reveal that DAP could be a promising therapeutic candidate for the treatment of IDD.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunopathological characteristics and therapeutic effects of UC-MSCs in a pigeon breeder's lung mouse model. UC-MSCs在养鸽人肺小鼠模型中的免疫病理特征及治疗作用。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-21 DOI: 10.3724/abbs.2025010

Jingran Xu, Li Li, Yaping Zhou, Zulipikaer Abudureheman, Lexin Xue, Chao Wu, Xiaoguang Zou

{"title":"Immunopathological characteristics and therapeutic effects of UC-MSCs in a pigeon breeder's lung mouse model.","authors":"Jingran Xu, Li Li, Yaping Zhou, Zulipikaer Abudureheman, Lexin Xue, Chao Wu, Xiaoguang Zou","doi":"10.3724/abbs.2025010","DOIUrl":"10.3724/abbs.2025010","url":null,"abstract":"Hypersensitivity pneumonitis (HP), including pigeon breeder's lung (PBL), often progresses from acute inflammation to fibrosis, impairing lung function and limiting targeted therapeutic strategies. Mechanistic studies on PBL progression are limited by the lack of preclinical animal models and a predominant focus on patient data. This study explores the immunopathological characteristics of all stages of PBL in mice and evaluates the therapeutic potential of human umbilical cord-derived mesenchymal stem cells (UC-MSCs) during the non-fibrotic stage. PBL models are created in A/J mice through tracheal instillation of pigeon dropping extract (PDE) protein powder. Different doses (0.4 × 10 6, 0.8 × 10 6, and 1.6 × 10 6 cells per animal) and frequencies (1-2 times) are administered to the model. The immunopathological characteristics of PBL and the therapeutic effects of UC-MSCs are assessed using micro-CT, pulmonary function, histopathology, cell counts in BALF, HYP levels, inflammatory factor levels, immunohistochemistry, and fibrosis marker expression in lung tissues. The results show that PDE exposure consistently impairs pulmonary function and increases the levels of inflammation and fibrosis markers as the disease progresses. Model mice experience non-fibrotic stages (acute inflammation) from days 0-36, mild fibrosis from days 37-77, and severe fibrosis from day 78 onwards. UC-MSCs, particularly at the highest dose (1.6 × 10 6 cells), effectively treat non-fibrotic PBL by improving pulmonary function (lung ventilation area recovers) and reducing inflammation and fibrosis. This study successfully establishes PBL mouse models reflecting both the acute (inflammatory) and chronic (fibrotic) stages, and UC-MSCs have the potential to delay fibrosis, providing new therapeutic options for PBL and other inflammation-induced lung fibrotic diseases.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"473-485"},"PeriodicalIF":3.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annexins: central regulators of plant growth and stress signaling. 膜联蛋白：植物生长和胁迫信号的中心调节因子。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-15 DOI: 10.3724/abbs.2024228

Chen Xie, Mingyue Zhu, Ruirui Shi, Liu Yang, Xiaoya An, Chao Wang

{"title":"Annexins: central regulators of plant growth and stress signaling.","authors":"Chen Xie, Mingyue Zhu, Ruirui Shi, Liu Yang, Xiaoya An, Chao Wang","doi":"10.3724/abbs.2024228","DOIUrl":"https://doi.org/10.3724/abbs.2024228","url":null,"abstract":"Annexins are a family of multifunctional calcium-dependent and phospholipid-binding proteins that are widely distributed in the plant kingdom. They have a highly conserved evolutionary history that dates back to single-celled protists. Plant annexins, as soluble proteins, can flexibly bind to endomembranes and plasma membranes, exhibiting unique calcium-dependent and calcium-independent characteristics. Members of the annexin family have diverse functions, including binding to F-actin, participating in ATP and GTP hydrolysis, and even serving as peroxidases or cation channels. Annexins play pivotal roles in plant growth and stress signaling. They can respond sensitively to environmental, metabolic, and developmental signals, thereby affecting cytoskeleton remodeling and exocytosis mechanisms. Plant annexin gene families have been successfully identified in multiple species, and their expression and intracellular localization are precisely regulated by developmental processes and environmental factors. Although research on plant annexins has aroused great interest, their depth and breadth still need further expansion compared with those of animal annexins. This article provides a comprehensive and in-depth review of the characteristics and functions of plant annexin families, revealing their core roles in plant growth and adaptation, and yielding valuable references and insights for future research.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF3 triggers M2 macrophage polarization to protect against pulp inflammation through WNT4 regulation. ATF3通过WNT4调控触发M2巨噬细胞极化，保护牙髓炎症。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-13 DOI: 10.3724/abbs.2025005

Liu Liu, Jie Wang, Jie Yu, Jing Wang, Jinhua Yu

{"title":"ATF3 triggers M2 macrophage polarization to protect against pulp inflammation through WNT4 regulation.","authors":"Liu Liu, Jie Wang, Jie Yu, Jing Wang, Jinhua Yu","doi":"10.3724/abbs.2025005","DOIUrl":"https://doi.org/10.3724/abbs.2025005","url":null,"abstract":"Pulpitis is a common inflammatory oral disease that can lead to pulp necrosis. The aim of this study is to investigate the expression and regulatory mechanisms of ATF3, a potential therapeutic marker, in pulpitis. A mouse pulpitis model with different degrees of inflammation is established, and the expression of ATF3 in pulpitis is explored. The histological features of healthy pulp and pulpitis are analyzed by HE staining, and classical inflammatory factors are detected by immunohistochemistry (IHC). In an in vitro study, we investigate the role of ATF3 in the regulation of WNT4 transcription and explore the effects of the ATF3/WNT4 axis on the polarization of RAW264.7 macrophages, the inflammatory response and the osteogenic differentiation of human dental pulp stem/stromal cells (hDPSCs). Our results show that ATF3 is expressed at low levels in inflamed pulp tissues; overexpression of ATF3 reduces the area of pulp necrosis, decreases the level of pro-inflammatory factors, and promotes macrophage polarization toward the M2 type. Furthermore, we reveal that ATF3 binds to the WNT4 promoter region and positively regulates the expression of WNT4 and that ATF3 downregulates M1 markers and increases the expression of M2 markers by regulating WNT4 expression. In addition, ATF3 promotes the osteogenic differentiation of dental pulp stem cells. In summary, this study reveals that ATF3 promotes M2 macrophage polarization by regulating WNT4, which in turn inhibits pulpal inflammatory responses and promotes the osteogenic differentiation of dental pulp stem cells. These findings suggest that ATF3 may be a potential target for pulpitis treatment.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of six commercially available angiotensin II type 1 receptor antibodies. 六种市售血管紧张素II型1受体抗体的验证。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-08 DOI: 10.3724/abbs.2024199

Bingjie Li, Xi Zhang, Fei Sun, Xingzhong Zhang, Huirong Liu, Suli Zhang

引用次数: 0

Decreased CCL5 expression in endometrial stromal cells induces deficient CCR5 ⁺CD4 ⁺ T cells in endometriosis. 子宫内膜间质细胞CCR5表达降低导致子宫内膜异位症患者CCR5 +CD4 + T细胞缺失。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-08 DOI: 10.3724/abbs.2024178

Yue Li, Yunyun Li, Yewei Lu, Yikong Lin, Xiaolin Wang, Yizhun Zhu, Qiongjing Zeng, Meirong Du

{"title":"Decreased CCL5 expression in endometrial stromal cells induces deficient CCR5 +CD4 + T cells in endometriosis.","authors":"Yue Li, Yunyun Li, Yewei Lu, Yikong Lin, Xiaolin Wang, Yizhun Zhu, Qiongjing Zeng, Meirong Du","doi":"10.3724/abbs.2024178","DOIUrl":"https://doi.org/10.3724/abbs.2024178","url":null,"abstract":"Endometriosis (EMS) is a benign gynecological disease characterized by the growth of endometrial tissue outside the uterine cavity. Evidence shows that the survival of patients with ectopic endometrial implants is associated with a dysregulated immune microenvironment. CD4 + T cells can regulate EMS through diverse cytokines, the inflammatory response, and angiogenesis. CCR5 +CD4 + T cells exhibit increased cellular immunogenicity and play a role in infectious diseases, host defense, and cancer progression. However, the specific mechanisms of CCR5 +CD4 + T cells in EMS remain unknown. In the present study, flow cytometry and RNA-seq are utilized to assess the proportions and features of CCR5 +CD4 + T cells in EMS patients, RT-PCR and ELISA are used to assess the production of CCL5 by ectopic endometrial stromal cells (ecESCs). Two EMS models are established through C57B6 wild-type and CCL5 ‒/‒ mice and utilized to explore the in vivo effects of CCR5 +CD4 + T cells on ectopic lesions. Compared with CCR5 ‒CD4 + T cells, CCR5 +CD4 + T cells display a more activated and cytotoxic phenotype. Diminished CCR5 +CD4 + T cells and their impaired ability to produce IFN-γ are observed in the ectopic lesions of EMS patients and in murine EMS models. Impaired production of CCL5 has been detected in human ecESCs. Moreover, endometria stripped from CCL5 ‒/‒ mice are more likely to generate ectopic lesions in the peritoneum of recipient mice. These findings demonstrate that the attenuated recruitment of CCR5 + CD4 + T cells in ectopic lesions caused by decreased production of CCL5 in ecESCs may facilitate the progression of EMS.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor occupancy limits DNA methylation and determines ICAM1 expression in breast cancer. 转录因子占用限制DNA甲基化并决定乳腺癌中ICAM1的表达。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2025-01-08 DOI: 10.3724/abbs.2024237

Mingcang Chen, Ying Zhou, Zhengwei Fu, Chunyu Wu

引用次数: 0