Acta biochimica et biophysica Sinica最新文献_第7页

Artemisinin alleviates arsenic-induced myocardial injury in rats by modulating oxidative stress and inflammatory responses. 青蒿素通过调节氧化应激和炎症反应减轻砷致大鼠心肌损伤。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024225

Wenjuan Qin, Yifei Zhou, Chuncui Chen, Xueting Guo, Ruimeng Tian, Ruoxi Chen, Wenrong Shi, Lei Huang, Caiyun Zhang, Shanshan Dong, Guilin Lu

引用次数: 0

Cellular functions and biomedical applications of circular RNAs. 环状rna的细胞功能和生物医学应用。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-24 DOI: 10.3724/abbs.2024241

Zheyu Zhang, Zefeng Wang

引用次数: 0

The host gene CSTF2 regulates HBV replication via HBV PRE-induced nuclear export. 宿主基因CSTF2通过HBV预诱导核输出调控HBV复制。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-23 DOI: 10.3724/abbs.2024216

Jinyu Wang, Jing Li, Wentao Xie, Zhongliang Shen, Jingwen Wu, Richeng Mao, Mengji Lu, Jiming Zhang

{"title":"The host gene CSTF2 regulates HBV replication via HBV PRE-induced nuclear export.","authors":"Jinyu Wang, Jing Li, Wentao Xie, Zhongliang Shen, Jingwen Wu, Richeng Mao, Mengji Lu, Jiming Zhang","doi":"10.3724/abbs.2024216","DOIUrl":"10.3724/abbs.2024216","url":null,"abstract":"The persistent global burden of hepatitis B virus (HBV) infection has prompted ongoing investigations into host determinants of viral control. In this study, we investigate the regulatory influence of the host gene cleavage stimulation factor subunit 2 (CSTF2) on HBV replication dynamics. We demonstrate differential CSTF2 expression across the spectrum of HBV infection phases, with upregulated expression noted during the immune-reactive and inactive carrier states compared with the immune-tolerant phase. Notably, dose-responsive attenuation of HBV DNA, as well as surface and core protein levels, is observed subsequent to CSTF2 overexpression, whereas HBV RNA levels remain unaffected. Upon HBV transfection, a notable alteration in CSTF2 subcellular localization is discerned, suggesting active relocalization to the cytoplasm, potentially mediated through interaction with the HBV posttranscriptional regulatory element (PRE). This interaction appears to impede the nuclear export of HBV RNA. Additionally, distinct antiviral efficacies are attributed to the functional domains of the CSTF2 protein, indicating a multifaceted host defense mechanism. These insights increase the understanding of host-virus interplay and identify CSTF2 as a candidate for antiviral therapeutic strategies.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"486-496"},"PeriodicalIF":3.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of USP22 by miR-200b-5p represses gastric cancer cell proliferation and migration by targeting the NF-κB signaling pathway. miR-200b-5p抑制USP22通过靶向NF-κB信号通路抑制胃癌细胞的增殖和迁移。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-20 DOI: 10.3724/abbs.2024231

Yingying Guo, Panpan Zhang, Zhixing Gao, Xiaotian Liu, Chen Su, Su Chen, Tao An, Jingjing Hou

{"title":"Inhibition of USP22 by miR-200b-5p represses gastric cancer cell proliferation and migration by targeting the NF-κB signaling pathway.","authors":"Yingying Guo, Panpan Zhang, Zhixing Gao, Xiaotian Liu, Chen Su, Su Chen, Tao An, Jingjing Hou","doi":"10.3724/abbs.2024231","DOIUrl":"https://doi.org/10.3724/abbs.2024231","url":null,"abstract":"Gastric cancer (GC) is an aggressive tumor type with an intricate pathogenesis and limited therapeutic options. Ubiquitin-specific protease 22 (USP22) is a protein implicated in cell proliferation, metastasis, and tumorigenesis. However, the regulatory mechanisms governing USP22 in GC are still not fully understood. In this study, we perform bioinformatics analysis to identify conserved miRNA recognition sites for miR-200b-5p within the 3'UTR of USP22. Validation via luciferase reporter assay confirms the transcriptional regulation of USP22 by miR-200b-5p. Overexpression of miR-200b-5p markedly inhibits the proliferation and migration of GC cells in vitro and suppresses tumor growth in vivo. Conversely, ectopic expression of USP22 reversed this effect by modulating the NF-κB signaling pathway. Additionally, qPCR analysis reveals an inverse correlation between the miR-200b-5p level and USP22 expression in GC. Collectively, our findings indicate that miR-200b-5p-mediated inhibition of USP22 attenuates cell proliferation by targeting the NF-κB signaling pathway in GC, suggesting that miR-200b-5p and USP22 could serve as potential diagnostic or therapeutic targets for gastric cancer and other related human diseases.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

O-GlcNAcylation-related genes mediate tumor microenvironment characteristics and prediction of immunotherapy response in gastric cancer. o - glcn酰化相关基因介导胃癌肿瘤微环境特征及免疫治疗反应预测

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-18 DOI: 10.3724/abbs.2024222

Wangwen Wang, Xi Lu, Chengjun Zhu, Jie Li, Yue Liu, Zhangchao Yao, Xiaolin Li

{"title":"O-GlcNAcylation-related genes mediate tumor microenvironment characteristics and prediction of immunotherapy response in gastric cancer.","authors":"Wangwen Wang, Xi Lu, Chengjun Zhu, Jie Li, Yue Liu, Zhangchao Yao, Xiaolin Li","doi":"10.3724/abbs.2024222","DOIUrl":"https://doi.org/10.3724/abbs.2024222","url":null,"abstract":"We aim to identify molecular clusters related to O-GlcNAcylation and establish a novel scoring system for predicting prognosis and immunotherapy efficacy in patients with gastric cancer (GC). The transcriptomic and clinical data are obtained from XENA-UCSC and GEO databases. The O-GlcNAcylation-related genes are obtained from the GSEA database. Consensus clustering analysis is employed to identify O-GlcNAcylation-related molecular clusters, and principal component analysis (PCA) is utilized to develop a novel prognostic scoring system for predicting GC outcomes and immunotherapy efficacy. The prognostic accuracy of the scoring system is assessed across five real-world cohorts. The biological function of actin alpha 2, smooth muscle (ACTA2) in GC is determined through experimental verification. Using 34 O-GlcNAcylation-related genes associated with prognosis in GC patients, these individuals are divided into two distinct subgroups characterized by different outcomes, tumor microenvironment profiles, and clinical case characteristics. The DEGs between the two subgroups are subsequently used to further divide the GC patients into two subgroups by consensus cluster analysis. PCA is used to construct a prognostic scoring system, which reveal that patients in the low-score subgroup have a better prognosis and greater benefit from immunotherapy. The accuracy of the scoring system is confirmed through validation in a cohort of patients receiving immunotherapy in the real world. ACTA2 promotes proliferation and inhibits apoptosis in GC cells. These findings suggest that we successfully establish molecular clusters associated with O-GlcNAcylation and develop a scoring system that demonstrates strong performance in predicting the prognosis of patients with GC and the effect of immunotherapy interventions.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATF4 promotes glutaminolysis and glycolysis in colorectal cancer by transcriptionally inducing SLC1A5. ATF4通过转录诱导SLC1A5促进结直肠癌中的谷氨酰胺解和糖酵解。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-18 DOI: 10.3724/abbs.2024226

Zengli Zhou, Shufang Ye, Jingyu Chen, Fei Dai, Luyi Chen, Ran Ye, Jianmei Zhang, Gefei Chen, Yanjiao Wang, Yangyang Liu

{"title":"ATF4 promotes glutaminolysis and glycolysis in colorectal cancer by transcriptionally inducing SLC1A5.","authors":"Zengli Zhou, Shufang Ye, Jingyu Chen, Fei Dai, Luyi Chen, Ran Ye, Jianmei Zhang, Gefei Chen, Yanjiao Wang, Yangyang Liu","doi":"10.3724/abbs.2024226","DOIUrl":"https://doi.org/10.3724/abbs.2024226","url":null,"abstract":"Glutaminolysis and glycolysis promote the malignant progression of colorectal cancer. The role of activating transcription factor 4 (ATF4) in solute carrier family 1 member 5 (SLC1A5)-mediated glutaminolysis and glycolysis remains to be elucidated. SLC1A5 and ATF4 expression levels are detected in colorectal cancer tissues. ATF4 is knocked down or overexpressed to assess its role in cell viability, migration and invasion. SLC1A5 is knocked down to evaluate its role in cell viability, migration, invasion, and metastasis and the metabolism of glutamine and glucose. The regulatory effect of the transcription factor ATF4 on SLC1A5 transcription and expression is determined using a luciferase reporter assay and chromatin immunoprecipitation (ChIP) techniques. Upregulated ATF4 and SLC1A5 expressions are observed in tumor tissue, which is positively correlated with the tumor, node, and metastasis (TNM) stages. ATF4-overexpressing SW480 cells show the increased cell viability, migration and invasion. Conversely, ATF4 knockdown decreases the viability, migration and invasion of HCT-116 cells. SLC1A5 knockdown inhibits viability, migration, invasion, and metastasis and the metabolism of glutamine and glucose in HT-29 cells, as well as the expressions of two key glycolytic enzymes, hexokinase 2 (HK2) and pyruvate kinase M2 (PKM2). The luciferase activity of the SLC1A5 promoter is increased by ATF4 overexpression. SLC1A5 promoter enrichment is increased by anti-ATF4 antibody immunoprecipitation in ATF4-overexpressing colorectal cells, indicating that ATF4 targets SLC1A5 to promote glutamine and glucose metabolism in these cells. In summary, the ATF4/SLC1A5 axis plays a significant role in the progression of colorectal cancer by regulating glutamine metabolism and glycolysis.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CD40 ligation-induced ERK activation leads to enhanced radiosensitivity in cervical carcinoma cells via promoting autophagy. CD40结扎诱导的ERK激活通过促进自噬导致宫颈癌细胞放射敏感性增强。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-18 DOI: 10.3724/abbs.2024229

Baocai Liu, Yadong Zhang, Quan Wang, Qian Wang, Zhixin Wang, Li Feng

引用次数: 0

CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1. CARF通过PABPC1调控小鼠精子发生过程中的选择性剪接和piwi/piRNA复合物。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-18 DOI: 10.3724/abbs.2024224

Yuming Cao, Shengnan Wang, Jie Liu, Jinfeng Xu, Yan Liang, Fei Ao, Zexiao Wei, Li Wang

{"title":"CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1.","authors":"Yuming Cao, Shengnan Wang, Jie Liu, Jinfeng Xu, Yan Liang, Fei Ao, Zexiao Wei, Li Wang","doi":"10.3724/abbs.2024224","DOIUrl":"https://doi.org/10.3724/abbs.2024224","url":null,"abstract":"ADP-ribosylation factor collaborator (CARF), which is also known as CDKN2AIP, was first recognized as an ADP-ribosylation factor-interacting protein that participates in the activation of the ARF-p53-p21 (WAF1) signaling pathway under different conditions, such as oxidative and oncogenic stresses. The activation of this pathway often leads to cell growth arrest and apoptosis as well as senescence. Previous studies revealed that CARF, an RNA-binding protein, is critical for maintaining stem cell pluripotency and somatic differentiation. Nevertheless, its involvement in spermatogenesis has not been well examined. In this study, we show that male mice deficient in Carf expression present impaired spermatogenesis and fertility. IP-MS and RNA-seq analyses reveal that CARF/ Carf interacts with multiple key splicing factors, such as PABPC1, and directly targets 356 different types of mRNAs in spermatocytes. Carf-associated mRNAs display aberrant splicing patterns when Carf expression is deficient. In addition, our results demonstrate that PIWIL1 expression and localization are altered in the Carf -/ - mouse model through the downregulation of PABPC1, which further affects the ratio of pachytene-piRNA. Our study suggests that CARF is critical for regulating alternative splicing in mammalian spermatogenesis and determining infertility in male mice.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-lipoic acid targets KLF7 expression to inhibit cervical cancer progression. α -硫辛酸靶向KLF7表达抑制宫颈癌进展

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-17 DOI: 10.3724/abbs.2024212

Yi Mao, Hongtao Li, Gang Xu, Jiazhen Tian, Yuechan Chen, Zhiwei Zhang

{"title":"Alpha-lipoic acid targets KLF7 expression to inhibit cervical cancer progression.","authors":"Yi Mao, Hongtao Li, Gang Xu, Jiazhen Tian, Yuechan Chen, Zhiwei Zhang","doi":"10.3724/abbs.2024212","DOIUrl":"10.3724/abbs.2024212","url":null,"abstract":"It is unclear what part KLF7 plays in cervical cancer. In this study, immunohistochemical and bioinformatics analyses reveal that KLF7 expression is lower in normal cervical tissues than in cervical cancer tissues, and the high level of KLF7 transcripts in cervical cancer tissues is negatively correlated with patients' overall and disease-free survival. In addition, KLF7 overexpression facilitates the proliferation, migration, and invasion of cervical cells, reduces PFKL expression, and increases the expressions of KLF4, Nanog, OCT4, CD44, SOX2, and ACADL. Additionally, knocking out the Exon 2 of KLF7 in HeLa cells results in a decrease in the total expression of KLF7 but an increase in the nuclear expression of KLF7, an increase in the capacity for proliferation, migration, invasion, and oncogenicity, and an increase in the density and ridge density of mitochondria. Consistent with these findings, RNA-seq analysis shows that knocking out the Exon 2 of KLF7 facilitates the expression of gene sets associated with cancer compared with that in wild-type HeLa cells. Moreover, the administration of alpha-lipoic acid (ALA) leads to a reduction in KLF7 expression in cells and tumor tissues, a suppression of the proliferation, migration, and invasion of HeLa and SiHa cells, and an increase in the carcinogenic potential of HeLa cells, while KLF7 overexpression shows the opposite effect on the expressions of ACADL and PFKL in HeLa and SiHa cells. In conclusion, KLF7 promotes the development of cervical cancer, and ALA can downregulate KLF7 expression and play a positive role in cervical cancer treatment.","PeriodicalId":6978,"journal":{"name":"Acta biochimica et biophysica Sinica","volume":" ","pages":"237-249"},"PeriodicalIF":3.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Buzhong Yiqi Decoction accelerates skeletal muscle regeneration. 补中益气汤促进骨骼肌再生。

IF 3.3 2区生物学

Acta biochimica et biophysica Sinica Pub Date : 2024-12-10 DOI: 10.3724/abbs.2024223

Tian Gao, Xiaodi Hu, Yingxi Chen, Qianni Yang, Xingchen Niu, Hu Li, Dahai Zhu, Ping Zeng, Yong Zhang, Dan Zhang

引用次数: 0