Acta Physiologiae Plantarum最新文献

{"title":"In vitro morphological and physiological traits of the rootstock GF677 (Prunus persica × P. amygdalus) under different blue–red LED intensities","authors":"Virginia Sarropoulou,&nbsp;Ilektra Sperdouli,&nbsp;Ioannis-Dimosthenis Adamakis,&nbsp;Aristarchos Konstantinidis,&nbsp;Katerina Grigoriadou","doi":"10.1007/s11738-024-03710-3","DOIUrl":"10.1007/s11738-024-03710-3","url":null,"abstract":"<div><p>Light (spectrum, intensity, photoperiod) is one of the most important factors that governs the proliferation of many plant species cultured in vitro<i>.</i> In this study, the in vitro shoot proliferation, photosynthetic pigments, photosystem II photochemistry, and leaf anatomy of the GF677 rootstock (<i>Prunus persica</i> x <i>Prunus amygdalus</i>) were investigated under three light intensities (20, 40 and 120 μmol m⁻² s⁻¹) provided by light-emitting diode (LED) lamps with a 430–690 nm absorption spectrum, emitted mainly by blue (21%) and red (63%) (BR, 1:3). The culture medium used was the MS supplemented with 3.1 μM benzyl adenine, 0.05 μM α-naphthalene acetic acid, 0.3 μM gibberellic acid, 20 g L⁻¹ sucrose (pH .8), and 6 g L⁻¹ Plant Agar (30 days, 16h photoperiod, 22 ± 2 °C). The results showed that shoot multiplication percentages (90.48–92.86%), proliferation rates (new shoots per explant) (1.95–2.10), shoot height (0.52 cm), maximum efficiency of photosystem II (PSII) photochemistry (<i>F</i>_v/<i>F</i>_m), and the efficiency of excitation energy capture by open PSII reaction centers (<i>F</i>_v′/<i>F</i>_m′) were significantly higher under 20 μmol m⁻² s⁻¹ and 40 μmol m⁻² s⁻¹ as compared to 120 μmol m⁻² s⁻¹. Moreover, under 20 μmol m⁻² s⁻¹, the photoprotective energy dissipation (Φ_NPQ), the quantum yield of non-regulated energy loss in PSII (Φ_NO), chlorophyll <i>b</i> (Chl <i>b</i>), total chlorophyll (<i>a</i> + <i>b</i>), and carotenoids were enhanced as compared to 40 μmol m⁻² s⁻¹, whereas the percentage of explants with macroscopically stress appearance symptoms (23.21%) and chlorophyll <i>a</i>/chlorophyll <i>b</i> (Chl <i>a</i>/Chl <i>b</i>) ratio were significantly lower under 20 μmol m⁻² s⁻¹. Leaves grown under 20 μmol m⁻² s⁻¹ exhibited a somehow typical dorsiventral leaf anatomy with distinguishable palisade and spongy parenchyma with more layers of palisade parenchyma as compared to the produced thin leaves with suppressed mesophyll tissue under 40 μmol m⁻² s⁻¹. Based on the results obtained, among the three different intensities tested, 20 μmol m⁻² s⁻¹ is the best and most beneficial lighting condition for GF677 proliferation.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using proteomic approaches to predict particulate matter stress response of ornamental plant","authors":"Arnon Setsungnern,&nbsp;Chairat Treesubsuntorn,&nbsp;Waleeporn Pongkua,&nbsp;Wararat Sriprapat,&nbsp;Sucheewin Krobthong,&nbsp;Yodying Yingchutrakul,&nbsp;Paitip Thiravetyan","doi":"10.1007/s11738-024-03714-z","DOIUrl":"10.1007/s11738-024-03714-z","url":null,"abstract":"<div><p>Previous studies reported that plants’ exposure to particulate matter (PM) may decrease the PM phytoremediation efficiency. This study aims to investigate the effect of PM on plants at the proteomic level. Here, five plant species were fumigated with cigarette PM (the initial PM_2.5 of 470–500 µg/m³) in a 15.6 L testing chamber for 6 days. PM removal efficiency and absorption were evaluated for protein pattern between plants exposed and non-exposure to PM for 24 h. The results showed that <i>Z. zamiifolia</i> and <i>S. trifasciata</i> can effectively remove indoor PM (35–40 μg/m³). Also, several plant proteins (heat shock, aconitate hydratase 3, chaperone protein, NADP-dependent malic enzyme, probably aquaporin TIP2-2, serine hydroxymethyltransferase 2, ATP-dependent zinc metalloprotease FTSH 8, V-type proton ATPase subunit B2, malate dehydrogenase, peptidyl-prolyl cis–trans isomerase, CYP38, peroxiredoxin-2E, and histone) reported significantly upregulated and downregulated about 100 times after PM exposure than control. Proteins related to water accumulation and H₂O₂ scavenging significantly changed (<i>p</i> value &lt; 0.05) after PM exposure. PM concentration was found low (&lt; 0.5 mg/m²) on the leaf surface after 150 h exposure. Herein, water accumulation and H₂O₂ scavenging protein might play an important role in plant PM stress.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the cell wall of flax phloem fibers during gravitropic reaction","authors":"Natalia Mokshina,&nbsp;Nadezda Ibragimova,&nbsp;Marina Ageeva,&nbsp;Polina Mikshina","doi":"10.1007/s11738-024-03718-9","DOIUrl":"10.1007/s11738-024-03718-9","url":null,"abstract":"<div><p>Flax (<i>Linum usitatissimum</i> L.) phloem fibers produce a tertiary cell wall as part of regular plant growth. The fiber tertiary cell wall is responsible for supporting the stem’s vertical position and is involved in the implementation of the negative gravitropic reaction when it is tilted. Rhamnogalacturonan I (RG-I) is one of the main matrix polysaccharides of the tertiary cell wall, playing an essential role in its functioning. The distribution of RG-I in fibers isolated from two stem sides (pulling and opposite) during graviresponse was studied. The increased content of galactose-containing polymers in the cell walls of fibers from the pulling stem sides was demonstrated. Differences in the distribution of RG-I epitopes on different sides of the stem were shown. RG-I with branched galactan side chains was found in the fiber tertiary cell walls; the labeling of such side chains in the cell wall of fibers from the pulling side of the stem was increased during gravistimulation. Using transcriptome analysis, the upregulation of genes encoding enzymes involved in RG-I metabolism (synthesis and modifications) in the fibers from the pulling stem side, mainly at the early stage of graviresponse, was revealed. A special role for fasciclin-like arabinogalactan proteins in response to mechanical stimuli was proposed. For the purpose of further modifying the plant cell wall properties, it would be worthwhile to have an understanding of the processes taking place in the fiber cell wall, both in normal conditions and during mechanical stress.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere microorganisms from the soil around the roots of Lycium barbarum L.","authors":"Zhi-bo Jiang,&nbsp;Ya-fei Ren,&nbsp;Yun-qi Hu,&nbsp;Jing-ling Yang,&nbsp;Ji-yuan Wei,&nbsp;Xiao-li Ma","doi":"10.1007/s11738-024-03720-1","DOIUrl":"10.1007/s11738-024-03720-1","url":null,"abstract":"<div><p>Plant of <i>Lycium barbarum</i> L., in Chinese name of “Goqi” tree, produce very important traditional Chinese medicine of Goqi berries for clinical application over 2000 years in China. In the early stage, people conducted extensive research on the bioactive components, planting methods, and deep processing of <i>L. barbarum</i> L., yet paid scant attention to the rhizosphere microorganisms surrounding their roots. In this context, the rhizosphere soil of <i>L. barbarum</i> L., collected in Ningxia province of China, was studied to investigate the microbial diversity by performing 16S rRNA/ITS sequencing, which led to identify 29 strains including 9 bacteria and 20 fungi. On the bases of the GNPS analysis of HPLC-q-ToF MS data of the fermentation broth, rich secondary metabolites were identified from twenty microorganisms. The molecular network clustering analysis suggests that the fermentation broth of bacteria may contain 607 compounds and that of fungi may contain 1359 compounds, involving multiple types. Antibacterial activity evaluation results revealed that rhizosphere microorganisms had inhibitory effects on <i>Staphylococcus aureus</i> and <i>Candida albicans</i>. This article lays a scientific foundation for the utilization of soil biological resources in <i>L. barbarum</i> L. cultivation and paves the way for the discovery of novel antibacterial active substances with unique structures.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of water and nutrient on the growth of Digitaria nuda obtained from areas without reported herbicide resistance","authors":"Wellington Costa da Silva,&nbsp;Frederico Monteiro Feijó,&nbsp;Siumar Pedro Tironi,&nbsp;Renan Cantalice de Souza,&nbsp;Gildemberg Amorim Leal Junior","doi":"10.1007/s11738-024-03716-x","DOIUrl":"10.1007/s11738-024-03716-x","url":null,"abstract":"<div><p>The occurrence of control failure after herbicide application suggests the emergence of tolerant <i>D. nuda</i> in sugarcane crops. However, the presence of species within the herbicide-resistant weed community before the selection process can be partially attributed to ecological adaptability. Biological studies of <i>Digitaria</i> spp. in areas without resistance reports are essential to understand the initial characteristics of populations before resistance events. In the present study, the predominance of <i>Digitaria</i> species was evaluated in ten areas of sugarcane cultivation without records of populations resistant to photosystem II-inhibiting herbicides belonging to four mills. First, we determined the sensitivity of <i>Digitaria</i> populations to herbicides. In addition, interspecific competitiveness and growth under nutrient and water availability conditions were determined for each identified species of <i>Digitaria</i>. The survey revealed a predominance of <i>D. horizontalis</i> and <i>D. nuda</i> in populations composed of <i>D. bicornis</i> and <i>D. ciliaris</i>, and all them were susceptible to herbicides. The combinations of competition between species showed no significant differences. <i>D. nuda</i> has a greater increase of aboveground biomass under water availability, which may favor its adaptation in areas with sugarcane straw left on the soil. The predominance of susceptible <i>D. nuda</i> in some populations is primarily due to its biological characteristics and its adaptation to cultivation practices. However, <i>D. nuda</i> plants obtained from the two susceptible populations showed differential responses to diuron, indicating diversity in populations in terms of herbicide response.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 10","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous melatonin and serotonin improve boron toxicity tolerance in tomato seedlings","authors":"Ufuk Celikkol Akcay,&nbsp;Esra Acar","doi":"10.1007/s11738-024-03712-1","DOIUrl":"10.1007/s11738-024-03712-1","url":null,"abstract":"<div><p>Boron toxicity is an abiotic stress restricting agricultural production in arid and semiarid parts of the world. This study aimed to investigate the effects of serotonin and melatonin on tomato seedlings under short (5 days) and long-term (8 days) boron toxicity applied as 7.5 mM boric acid in the nutrient solution. Shoot and root lengths, dry weights and water contents, ion leakage levels, malondialdehyde, proline and relative water contents were used as morphological and physiological stress indicators. Expression levels of the enzymatic antioxidative defense genes, <i>FeSOD</i>, <i>CAT2</i>, <i>GR1</i>, <i>APX1</i>, <i>P5CS</i>, ethylene biosynthesis gene <i>ACS2</i>, <i>DEADBOX</i> RNA helicase, and two protein kinase genes <i>CPK2 </i>and <i>MPK3 </i>were also investigated. Melatonin application (10 µM) completely reversed necrotic and chlorotic lesions on leaves, while serotonin application (5 µM) partly ameliorated the visible boron toxicity symptoms. Both indoleamines reduced membrane damage and increased osmoprotectant proline levels under long-term boron toxicity. Exogenous melatonin and serotonin applications also reduced <i>ACS2</i> gene expression while increasing the transcript levels of <i>CPK2</i>, as well as enzymatic antioxidative defense system genes under long-term boron toxicity. The study showed that both indoleamine compounds interacted with early and late stress responses and successfully mitigated boron toxicity stress in tomato.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant growth-promoting rhizobacteria affect growth and sulforaphane content in broccoli seedlings (Brassica.oleracea var. Italica)","authors":"Luz María Isabel Miguel-Cruz,&nbsp;Marco Polo Carballo-Sanchez,&nbsp;Juan José Almaraz-Suarez,&nbsp;Rubén San Miguel-Chávez","doi":"10.1007/s11738-024-03706-z","DOIUrl":"10.1007/s11738-024-03706-z","url":null,"abstract":"<div><p>In this study, the effect of inoculation with PGPR on growth, sulfur and DL-sulforaphane content of broccoli seedlings was evaluated. Rhizobacterial strains of <i>Bacillus pumilus</i> R44, <i>Pseudomonas tolaasii</i> P61 and <i>P. tolaasii</i> A46 were screened for total indole production and P solubilization. Broccoli seedlings were inoculated with these strains in order to establish a greenhouse experiment. Seedlings were harvested 30 days after inoculation to determine fresh weight, dry weight, root volume, height, and relative chlorophyll content, also S uptake and sulforaphane content. Treatment with PGPR resulted in an increase in almost all the variables evaluated compared to the control. Outstanding results were obtained with the A46 and R44 strains, being 18 and 49% higher for sulforaphane content. P61 was the strain with the best results related to indole production (384% higher than A46), phosphate solubilization (287% higher than A46), and root volume (44% higher than control). There were no significant differences in relative chlorophyll content and sulfur accumulation among the treatments. This is the first work that showed the positive effect of PGPR on sulforaphane content in broccoli seedlings, a compound reported with anticancer activity.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of harmful effects of prolong storage at low temperature in harvested pomegranate fruit by exogenous proline","authors":"Sanaz Molaei,&nbsp;Ali Soleimani,&nbsp;Vali Rabiei,&nbsp;Farhang Razavi","doi":"10.1007/s11738-024-03711-2","DOIUrl":"10.1007/s11738-024-03711-2","url":null,"abstract":"<div><p>The effect of exogenous administration of two concentrations of proline (10 and 20 mM) on postharvest values and chilling injury (CI) of ‘Malaseh Saveh’ pomegranate (<i>Punica granatum</i> L.) fruits was investigated during 90 days at 4 °C. Proline treatment attenuated CI symptoms and prevented membrane lipids peroxidation, and reduction of cell membrane integrity in treated fruits, which were represented by malondialdehyde and electrolyte leakage, respectively. Therewith, a higher amount of endogenous proline (352.48 µg g⁻¹ FW) was perceived in 20 mM proline-treated fruits at the end of the cold storage time. Furthermore, proline treatment increased antioxidant capacity and antioxidant enzyme activity, namely ascorbate peroxidase, superoxide dismutase, and catalase, and also retained ascorbic acid amount at higher levels. This treatment decreased the activity of polyphenol oxidase and enhanced the phenylalanine ammonia-lyase activity which led to high accumulation of total phenol, flavonoids, and anthocyanin. All in all, exogenous application of proline, especially 20 mM concentration, as a safe, natural, and environment-friendly osmolyte substance, could alleviate harmful effects of CI and retain eating values of pomegranate fruits all over long-term postharvest and shelf life.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in leaf N allocation and mesophyll conductance to CO2 in four tree species under low soil P stress in subtropical China","authors":"Jingchao Tang,&nbsp;Baodi Sun,&nbsp;Ruimei Cheng,&nbsp;Zuomin Shi,&nbsp;Da Luo,&nbsp;Shirong Liu,&nbsp;Mauro Centritto","doi":"10.1007/s11738-024-03707-y","DOIUrl":"10.1007/s11738-024-03707-y","url":null,"abstract":"<div><p>Low P (LP) levels in leaves can affect their photosynthetic N-use efficiency (PNUE), internal N allocation, and mesophyll conductance to CO₂ (<i>g</i>_m). The changes in leaf internal N allocation and <i>g</i>_m in N-fixing trees and the consequent changes in PNUE under low soil P treatments are not well understood. In this study, we exposed seedlings of <i>Dalbergia odorifera</i>, <i>Erythrophleum fordii</i> (N-fixing trees), <i>Castanopsis hystrix</i>, and <i>Betula alnoides</i> (non-N-fixing trees) to three levels of soil P. The effects were not consistent among species, and LP had no specific effect on N-fixing species. Saturated net CO₂ assimilation rate (<i>A</i>_sat) values in <i>D. odorifera</i> and <i>C. hystrix</i> were remarkably lower under LP than under high P (HP) because <i>C</i>_c in <i>D. odorifera</i> and <i>V</i>_cmax and <i>J</i>_max in <i>C. hystrix</i> were reduced. <i>N</i>_area values in <i>D. odorifera</i> and <i>C. hystrix</i> were also reduced under LP, and the degree of reduction of <i>N</i>_area was larger than that of <i>A</i>_sat, which resulted in decreased PNUE in these species. <i>P</i>_R and <i>g</i>_m in <i>D. odorifera</i> and <i>P</i>_R, <i>P</i>_B, and <i>g</i>_m in <i>C. hystrix</i> significantly decreased under LP and were internal factors affecting the variation in PNUE in these two trees. <i>P</i>_CW was significantly and linearly related to <i>P</i>_R only in <i>C. hystrix</i>, indicating that more N was invested in the cell walls to resist the damage caused by low soil P, at the expense of Rubisco N. Our results showed that soil P deficiency affected leaf N utilization, photosynthetic efficiency, and seedling growth.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crosstalk mechanism of hydrogen sulfide and nitric oxide in regulating the nickel induced toxicity of cyanobacteria Nostoc muscorum and Anabaena sp.","authors":"Garima Singh,&nbsp;Sheo Mohan Prasad","doi":"10.1007/s11738-024-03705-0","DOIUrl":"10.1007/s11738-024-03705-0","url":null,"abstract":"<div><p>The present study examined the interactive role of hydrogen sulfide (H₂S) and nitric oxide (NO) in alleviation of nickel (Ni) induced toxicity on growth, photosynthetic pigments, PS II photochemistry, and impact on oxidative stress biomarkers and antioxidant defense systems of cyanobacteria, i.e., <i>Nostoc muscorum</i> and <i>Anabaena</i> sp., respectively. To substantiate the potential function of H₂S [as sodium hydrosulfide (NaHS); 8 µM)] and NO [as sodium nitroprusside (SNP); 10 µM] test organisms was exposed under 1 µM Ni stress and distinct physio-biochemical parameters was evaluated. The Ni stress declined the growth (by 19% and 23% in <i>N. muscorum</i> and <i>Anabaena</i> sp. respectively), photosynthetic pigments, photosynthetic oxygen evolution, and imbalanced the values of chlorophyll <i>a</i> fluorescence kinetics parameters (Phi_Po, Psi_o, Phi_Eo, PI_ABS decreased together with Fv/Fo) whereas, energy flux parameters were increased (ABS/RC, TRo/RC, ETo/RC and DIo/RC). The exogenous supplementation of NaHS and SNP significantly reduced reactive oxygen species (ROS) in both the cyanobacteria and induced substantial improvement in growth and photosynthesis under Ni stress. Besides this, under NaHS and SNP treatment the activity of antioxidant enzymes like superoxide dismutase, peroxidase, catalase and glutathione-s-transferase downregulated the level of oxidative biomarkers (superoxide radicals, hydrogen peroxide and malondialdehyde equivalents). Further, the interplay role of H₂S and NO was evaluated using H₂S scavenger [hypotaurine (HT; 20 µM)] and inhibitor [propargylglycine (PAG; 50 µM)] of H₂S and scavenger of NO [2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO; 20 µM)] and NO inhibitor [NG-nitro-L-arginine methyl ester (L-NAME; 100 µM)]. The present study points towards the combined mechanism with downstream signaling of H₂S on NO mediated response in alleviation of Ni induced toxicity in rice field cyanobacteria.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":"46 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}