Acta Physiologiae Plantarum最新文献

{"title":"Protective effect of nitric oxide on lettuce plants subjected to salt stress in semi-hydroponic system","authors":"Isabelly Cristina da Silva Marques, João Domingos Rodrigues, Elizabeth Orika Ono, Dayane Mércia Ribeiro Silva, Geane Lourenço Bispo, Francisco de Assis de Oliveira","doi":"10.1007/s11738-023-03645-1","DOIUrl":"https://doi.org/10.1007/s11738-023-03645-1","url":null,"abstract":"<p>The use of low-quality water, with high salt concentrations, can cause several changes in plant metabolism, requiring strategies to facilitate its use in agriculture. Nitric oxide (NO) is an alternative among biological or synthetic substances that has promising effects on the physiological and biochemical responses of plants under salt stress conditions, as it promotes acclimation and favors plant growth. The aim of this research was to evaluate the effect of NO on lettuce plants grown under salt stress. A randomized block experimental design was used in a 5 × 2 factorial arrangement consisting of five concentrations of sodium nitroprusside (SNP) (0, 50, 100, 150, and 200 µM) and two salinity levels of the irrigation water (0.2 and 3.5 dS m⁻¹: without and with salt stress, respectively). SNP concentrations were applied weekly (foliar application), totaling three applications during the crop cycle. The variables analyzed included: chlorophyll <i>a</i> fluorescence, lipid peroxidation, hydrogen peroxide, antioxidant enzymes, and proline content. The foliar application of NO by SNP donor, at concentrations between 100 and 150 µM provided greater efficiency of energy use in the photosystems and stimulated plant defense by increasing enzyme activity and proline contents, promoting cell detoxification. Therefore, exogenous application of NO, by donors, to plants under low-quality water conditions is important to mitigate the harmful effects of salt stress.</p>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139509322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin supplementation alleviates drought stress in peach (Prunus persica) seedlings by improving photosynthesis, root morphological traits, and antioxidant defense system","authors":"Shahid Iqbal,&nbsp;Faisal Hayat,&nbsp;Mujahid Hussain,&nbsp;Naveed Mushtaq,&nbsp;Muneer Rehman,&nbsp;Alaiha Asif,&nbsp;Ummara Khan,&nbsp;Muhammad Adnan Shahid","doi":"10.1007/s11738-023-03634-4","DOIUrl":"10.1007/s11738-023-03634-4","url":null,"abstract":"<div><p>Plant yield and productivity are being impacted significantly by global climate change. Since water scarcity is one of the main risks to agriculture's future, developing plants that can withstand water stress is crucial. The peach is an essential fruit crop for examining how plants respond to drought stress, which lowers crop performance through yield and quality losses. Melatonin, a versatile, beneficial stress-relieving hormone, can be used to alter how plants respond to environmental stresses. The current investigation examined how melatonin may improve early peach seedlings' resistance to drought stress. Our findings revealed a significant decrease in plant biomass and photosynthetic activity and increased oxidative damage under drought stress. Melatonin application promotes plant development and reduces oxidative damage by increasing photosynthetic activity and chlorophyll contents. Melatonin also increases soluble sugars and proline contents. Furthermore, an increase in the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) was also recorded in peach seedlings. According to the results of our investigation, melatonin applied at a concentration of 100 µM had the highest effect on increasing plant survival under drought stress and contributed to the development of a practical approach for peaches under drought stress conditions.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139090594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenylalanine affects betalain biosynthesis and promotes ascorbic acid, α-tocopherol, and retinol accumulation in Amaranthus tricolor seedlings","authors":"Monika Kumari,&nbsp;Hidam Bishworjit Singh,&nbsp;Mohammad Imtiyaj Khan","doi":"10.1007/s11738-023-03629-1","DOIUrl":"10.1007/s11738-023-03629-1","url":null,"abstract":"<div><p>Betalains are health-promoting plant pigments accumulated in non-anthocyanic plants. Owing to its limited distribution in nature, metabolic trade-offs of inhibiting them are poorly understood. The aim of this study was to investigate the effects of inhibition of betalains in <i>Amaranthus tricolor</i> seedlings to gain insights into the relationship of betalain biosynthesis with other biosynthetic pathways in betalain-accumulating plants. Phenylalanine (Phe; 12.5, 25, and 50 mmol L⁻¹) and 3-methyl-2-benzothiazolinone hydrazone (MBTH) (25 mmol L⁻¹) were treated to inhibit betalain biosynthesis in ten-day-old <i>A. tricolor</i> (red) seedlings. After two and six days of treatment, target (betalain-related metabolites) and non-target metabolites were analyzed. In two days, Phe content increased by 2.6, 8.5, and 17.4-fold in Phe (12.5, 25, and 50 mmol L⁻¹)-treated seedlings, respectively, compared to control, indicating the uptake of Phe by the seedlings. Phe treatment led to a 10.1–18% decrease in betacyanins, while MBTH caused an 18.3% decrease in two days. In both treatments, <i>cyclo</i>-DOPA formation, which is essential for betacyanin biosynthesis, seems to be inhibited, albeit through different mechanisms. Betalain biosynthetic precursors and intermediates, viz., tyrosine, L-DOPA, and dopamine decreased differentially. Ascorbic acid, <i>α</i>-tocopherol, and retinol contents increased in both treatments concomitant with the reduction in betalains, total phenols, and antioxidant enzymes. Therefore, Phe treatment is beneficial in enhancing antioxidant metabolites in betalain-accumulating plants. However, the mechanism of increasing ascorbic acid on inhibiting betalains needs further investigation in other betalain-producing plants also to understand if ascorbic acid is involved in regulating betalain biosynthesis.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal concentration of melatonin enhances drought stress tolerance in fenugreek","authors":"Hamzeh Amiri,&nbsp;Zohreh Zamani,&nbsp;Marino B. Arnao,&nbsp;Ahmad Ismaili,&nbsp;Parvaneh Hemmati Hassan Gavyar,&nbsp;Hamed Khodayari","doi":"10.1007/s11738-023-03643-3","DOIUrl":"10.1007/s11738-023-03643-3","url":null,"abstract":"<div><p>Fenugreek (<i>Trigonella foenum-graecum</i> L.) is a valuable medicinal plant in farm and pharmaceutical industry, and on the other hand, drought stress is one of the major problems of crop production in most countries. To explore the effect of drought stress on fenugreek, we first examined three levels of water deficit (− 3.0, − 5.0, − 7.0 Bar water potentials using polyethylene glycol) and assessed plant responses to polyethylene glycol-induced stress. In the second experiment, the effect of different concentrations of melatonin (50, 100, 300 and 500 µM) on the alleviation of detrimental effects of water stress was investigated in the highest level of drought stress (− 7 Bar PEG). The finding in the first experiment (drought stress applying) demonstrated that water deficit decreased length, fresh and dry weight of shoot and root, chlorophyll content and antioxidant enzyme activities, such as ascorbate peroxidase, catalase, superoxide dismutase and peroxidase, and also led to an increase in proline content. Also, melatonin treatment (especially 100 mM) improved photosynthetic performance by increase in net photosynthesis (P_N) and transpiration rate (E), reducing chlorophyll degradation but increasing the antioxidant enzyme activities. These results revealed that fenugreek tolerated water deficit by increasing the endogenous melatonin and trigonelline, as well as the general physiological responses.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138947712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological, biochemical, and antioxidant responses of oregano subspecies (Origanum vulgare L. ssp. gracile and ssp. vulgare) to NaCl stress","authors":"Zahra Azimzadeh,&nbsp;Abbas Hassani,&nbsp;Babak Abdollahi Mandoulakani,&nbsp;Ebrahim Sepehr","doi":"10.1007/s11738-023-03636-2","DOIUrl":"10.1007/s11738-023-03636-2","url":null,"abstract":"<div><p>Salinity is an outstanding barrier against the production of agricultural crops, especially in arid and semi-arid regions. Oregano (<i>Origanum vulgare</i> L.), a valuable herb of the Lamiaceae family, contains various types of biologically active constituents such as essential oils, tannins, resins, sterols, flavonoids, and phenolic glycosides. The present research was carried out to investigate the influence of salinity stress on some physiological and biochemical attributes and antioxidant responses in two oregano subspecies (ssp. <i>vulgare</i> and ssp. <i>gracile</i>). Salt treatments were applied using irrigation with different sodium chloride concentrations (0, 25, 50, and 100 mM NaCl). The results revealed a remarkable decline in relative water content (RWC) and photosynthetic pigments in both subspecies under NaCl stress. Total soluble sugars (TSS) decreased in plants exposed to severe salt stress (100 mM NaCl), whereas H₂O₂ production, electrolyte leakage (EL), malondialdehyde (MDA), and leaf proline contents increased in these plants compared to control plants. A positive relationship was found between H₂O₂ production with EL and MDA. Furthermore, salinity improved phenolic content, antioxidant capacity, and activity of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) enzymes. The highest total flavonoid content (TFC) was achieved at 50 mM NaCl salinity, which increased by 19.33% compared to control plants. A positive relationship between the activity of phenylalanine ammonia-lyase (PAL) and TPC and TFC was observed. Analysis of phenolic compounds by HPLC showed that the amounts of gallic acid, caffeic acid, chlorogenic acid, and quercetin in ssp. <i>gracile</i> and caffeic acid, cinnamic acid, and quercetin in ssp. <i>vulgare</i> significantly increased with increasing salinity stress. In general, the findings of this study demonstrated that oregano subspecies ameliorate salt-induced osmotic and oxidative damages through increasing proline accumulation, antioxidant enzymes activity, and secondary metabolites production.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138944413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-photochemical quenching triggered by abscisic acid mitigates the inhibition of photosynthesis in rice under drought","authors":"Xia Zhao,&nbsp;Baohua Feng,&nbsp;Ranfang Liu,&nbsp;Guanfu Fu","doi":"10.1007/s11738-023-03633-5","DOIUrl":"10.1007/s11738-023-03633-5","url":null,"abstract":"<div><p>Abscisic acid (ABA) plays a key role in drought. Non-photochemical quenching (NPQ), as a photoprotective mechanism, also plays an important role in different adversities. Although this mechanism is relatively understood, the relationship between ABA synthesis and NPQ induction under drought remains poorly defined. To understand the role of ABA in the mechanism underlying the photosynthetic response to drought, two rice cultivars with different chlorophyll contents were exposed to drought and exogenous ABA. Drought significantly stimulated the accumulation of endogenous ABA in leaf, and the application of exogenous ABA under drought further stimulated it through the up-regulated expression of the genes encoding 9′-<i>cis</i>-epoxycarotenoid dioxygenase. The application of exogenous ABA triggered higher NPQ under drought, especially in Zhefu802. The expression of <i>PsbS1</i> was significantly increased by drought, while the expression of <i>PsbS1</i> was further up-regulated by spraying ABA under drought. However, the high zeaxanthin content was not further up-regulated by spraying ABA under drought. These results indicated that ABA increased the expression of <i>PsbS1</i> and further triggered NPQ as a strategy to decrease the overexcitation of the photosynthetic apparatus in rice. This phenomenon was validated by slowing the decrease of the net photosynthetic rate under the application of exogenous ABA in the two rice cultivars. Except for the regulation of photoprotection, exogenous ABA significantly decreased the malondialdehyde level in Zhefu802 and the H₂O₂ level in Fgl. In conclusion, exogenous ABA significantly increases endogenous ABA and then triggers NPQ to mitigate the inhibition of photosynthesis in drought.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138947053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of water-deficit tolerance in Upland cotton (Gossypium hirsutum L.) assessing morphological, biochemical, molecular and yield attributes","authors":"Muhammad Saeed,&nbsp;Muhammad Naeem,&nbsp;Alia Javed,&nbsp;Shagufta Perveen,&nbsp;Iqra Sajjad,&nbsp;Muhammad Zaid Yousaf,&nbsp;M. Shahid Munir Chohan,&nbsp;Muhammad Riaz,&nbsp;Sana Ullah,&nbsp;Xianliang Song","doi":"10.1007/s11738-023-03641-5","DOIUrl":"10.1007/s11738-023-03641-5","url":null,"abstract":"<div><p>Upland cotton (<i>Gossypium</i> <i>hirsutum</i> L.) is an important crop in the world due to its natural fiber production. As a result of climate change, its production is affected due to frequent occurrence of water-deficit conditions during its growth period. Five cotton varieties, namely FH-114, FH-142, FH-152, CIM-473 and CIM-496 were evaluated under limited irrigation conditions to characterize water-deficit tolerance. The data were recorded for plant height, content of biochemical parameters (carotenoid, chlorophyll, wax, proline) and seed cotton yield per plant. The plant material was genotyped with 23 highly polymorphic simple sequence repeats markers. The cotton varieties, CIM-473 and FH-142, with higher content of carotenoid, chlorophyll, epicuticular wax, and proline maintained sustained vegetative growth and development due to osmoregulation; optimal photosynthesis and photochemical efficiency; and maintenance of balance in the C/N metabolism and energy homeostasis; which resulted in better seed cotton yield (&gt; 30% than the drought susceptible varieties) under limited irrigation conditions. Significant associations of simple sequence repeat DNA markers with plant height, carotenoid content, chlorophyll content, wax content, and seed cotton yield were identified. Especially, the DNA markers BNL1153 (chromosome 25) and BNL3031 (chromosome 9) might be the markers flanking the genes involved in the biosynthesis of proteins/enzymes (such as Late Embryogenesis Abundant proteins; chaperones; antioxidants; L-ascorbate peroxidase; enzymes in the ABA, proline, carotenoids and epicuticular wax biosynthesis) catalyzing the metabolic pathways leading to water-deficit tolerance in cotton and thus would be valuable resources for molecular breeding programs to develop water-deficit-tolerant cotton cultivars.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138818544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome analysis of Iris japonica var. variegata for chlorophyll and photosynthesis-related pathways involved in white–green striped leaves","authors":"Xiaofang Yu,&nbsp;Linjie Yue,&nbsp;Qingling He,&nbsp;Shurui Li,&nbsp;Yunhao Zheng,&nbsp;Zhiwen Wang,&nbsp;Heting Wang,&nbsp;Rouzhi Liu,&nbsp;Xin Huang,&nbsp;Xue Xiao,&nbsp;Lijuan Yang,&nbsp;Ting Lei","doi":"10.1007/s11738-023-03638-0","DOIUrl":"10.1007/s11738-023-03638-0","url":null,"abstract":"<div><p>As a well-known garden plant, many beautiful iris flower phenotypes and leaf variegation have been created. <i>Iris japonica</i> var. <i>variegata</i> is a variant of <i>I. japonica</i> that has a white–green striped leaf color genotype. It has high horticultural value, but its underlying molecular mechanisms remain unclear. Here, we analyzed the physiological features and ultrastructural observation of white and green tissues. In our study, the results showed that the phenotype was related to the abnormal chloroplast morphology and deficiencies in chlorophyll. To understand the mechanisms for these observations, we used Illumina sequencing technology to identify the transcriptome and profile differentially expressed genes (DEGs). We identified 1327 DEGs in white leaf tissues compared with green leaf tissues, including 342 upregulated genes and 985 downregulated DEGs. The downregulated DEGs included porphyrin and chlorophyll metabolism, chloroplast development, and photosynthetic pathways. Changes in the expression of genes (<i>CYSG</i> and <i>GLK</i>) involved in these pathways could be responsible for the phenotype of <i>I. japonica</i> var. <i>variegata</i>. To our knowledge, this is the first time that evidence has been provided to better understand the primary cause of chloroplast structure in white–green striped leaves for iris and will be valuable for the iris breeding industry.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138742995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative assessment between liquid culture in static and temporary immersion systems on multiplication, morpho-physiological and biochemical characteristics of Bambusa vulgaris Schrad. ex Wendl. shoots","authors":"Yudith García-Ramírez,&nbsp;Marisol Freire-Seijo,&nbsp;Raúl Barbón Rodríguez,&nbsp;Sinesio Torres Garcia","doi":"10.1007/s11738-023-03637-1","DOIUrl":"10.1007/s11738-023-03637-1","url":null,"abstract":"<div><h3>Key message</h3><p>Shoots of <i>B. vulgaris</i> cultured in TIS showed better morpho-physiological and biochemical characteristics, without hyperhydricity during in vitro multiplication.</p><h3>Abstract</h3><p>The aim of this study was to compare two in vitro culture methods: the liquid culture in static and temporary immersion system (TIS) and in terms of shoot multiplication and morpho-physiological response of shoots of <i>Bambusa vulgaris</i> Schrad. ex Wendl. The morpho-physiological parameters such as number of shoots, shoot length and number of leaves per shoot were measured οn the plants cultivated in vitro. In addition, water content, total phenolic content, and lignin content were determined οn the shoots developed under both conditions. The results of this study show that the use of TIS favored the propagation, morphology and biochemistry of the shoots grown in TIS. In this system, the length, number of shoots and leaves per explant increased. Likewise, the highest values for phenolic and lignin content were obtained. However, shoots grown in static liquid culture medium experienced an increase in water content and hyperhydricity. Lignin and total phenolic content also decreased. This study shows that in vitro multiplication of shoots at TIS can be a successful alternative to the conventional liquid culture system, while maintaining morphological and biochemical quality for reforestation purposes.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic variation in nitrogen use efficiency trait in 35 cucumber inbred lines","authors":"Ali Mirhosseyni,&nbsp;Moazzam Hassanpour Asil,&nbsp;Jamal-Ali Olfati,&nbsp;Mohammad Bagher Farhangi","doi":"10.1007/s11738-023-03642-4","DOIUrl":"10.1007/s11738-023-03642-4","url":null,"abstract":"<div><p>Nitrogen is an essential nutrient for plant growth and yield, however excess nitrogen fertilizer application can lead to detrimental environmental impacts. Identifying cucumber genotypes with high nitrogen use efficiency (NUE) could facilitate sustainable crop production through optimized nitrogen management. This study was conducted in March 2021 at the research greenhouse of Department of Horticultural Sciences, University of Guilan, Iran. The objective of this study was to assess growth, yield components, nitrogen accumulation, NUE, and nitrate reductase activity in 60 cucumber genotypes under sufficient (NS) and deficient (ND) nitrogen conditions. Nitrogen deficiency caused reductions in growth and yield across all genotypes, although the severity varied. Nitrogen content was higher in all plant organs under NS compared to ND. NUE and its components nitrogen uptake efficiency (NUpE) and nitrogen utilization efficiency (NUtE) were significantly higher under ND versus NS. Line 57 exhibited the best NUE and yield under both NS and ND, and could be considered a nitrogen-efficient genotype. Based on NUE performance, lines were categorized as efficient, semi-efficient, low-efficient, and inefficient. Nitrate reductase activity was higher in leaves versus roots, and positively correlated with NUE, suggesting a relationship between enzyme activity and nitrogen uptake efficiency in roots. This study demonstrates genotype-specific responses to nitrogen availability in cucumber. The identification of genotypes efficient in NUE, such as line 57, can inform breeding efforts to improve nitrogen responsiveness. Characterizing nitrogen responsiveness across genotypes also allows for optimized, genotype-specific nitrogen management recommendations to be made, facilitating sustainable crop production.</p></div>","PeriodicalId":6973,"journal":{"name":"Acta Physiologiae Plantarum","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138743103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}