Acta histochemica最新文献_第3页

Cryopreservation and passaging optimization for Galea spixii (Wagler, 1831) adult skin fibroblast lines: A step forward in species management and genetic studies Galea spixii (Wagler, 1831) 成体皮肤成纤维细胞系的低温保存和传代优化：在物种管理和基因研究方面向前迈进了一步。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-26 DOI: 10.1016/j.acthis.2024.152185

Leonardo Vitorino Costa de Aquino , Samara Lima Olindo , Yara Letícia Frutuoso e Silva , Lhara Ricarliany Medeiros de Oliveira , Yasmin Beatriz França Moura , Ana Lívia Rocha Rodrigues , Érika Almeida Praxedes , Moacir Franco de Oliveira , Alexandre Rodrigues Silva , Alexsandra Fernandes Pereira

{"title":"Cryopreservation and passaging optimization for Galea spixii (Wagler, 1831) adult skin fibroblast lines: A step forward in species management and genetic studies","authors":"Leonardo Vitorino Costa de Aquino , Samara Lima Olindo , Yara Letícia Frutuoso e Silva , Lhara Ricarliany Medeiros de Oliveira , Yasmin Beatriz França Moura , Ana Lívia Rocha Rodrigues , Érika Almeida Praxedes , Moacir Franco de Oliveira , Alexandre Rodrigues Silva , Alexsandra Fernandes Pereira","doi":"10.1016/j.acthis.2024.152185","DOIUrl":"10.1016/j.acthis.2024.152185","url":null,"abstract":"<div><h3>Background</h3><p><em>In vitro</em> culture of fibroblasts is a technique based on cell isolation, physiological characterization, and cryopreservation. This technique has not been described for <em>Galea spixii</em>, therefore, it can be used to learn about its cellular biology and genetic diversity.</p></div><div><h3>Objective</h3><p>We established fibroblast lines of six <em>G. spixii</em> individuals from several passages (second, fifth, eighth, and tenth) and cryopreserved them.</p></div><div><h3>Methods</h3><p>Fibroblasts recovered from skin biopsies were identified based on morphology, immunocytochemistry, and karyotyping. The cells were analyzed for morphology, ultrastructure, viability, proliferation, metabolism, oxidative stress, bioenergetic potential, and apoptosis before and after cryopreservation.</p></div><div><h3>Results</h3><p>After the eighth passage, the fibroblasts showed morphological and karyotypic changes, although their viability, metabolism, and proliferation did not change. An increase in oxidative stress and bioenergetic potential from the fifth to the eighth passages were also observed. Post cryopreservation, cell damage with respect to the ultrastructure, viability, proliferative rate, apoptotic levels, oxidative stress, and bioenergetic potential were verified.</p></div><div><h3>Conclusion</h3><p>Fibroblasts up to the tenth passage could be cultured <em>in vitro</em>. However, cells at the fifth passage were of better quality to be used for reproductive techniques. Additionally, optimization of the cryopreservation protocol is essential to improve the physiological parameters of these cells.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141764809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells 从人脐带间充质干细胞中提取的外泌体刺激小鼠毛发再生。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-24 DOI: 10.1016/j.acthis.2024.152184

Yang Jiao , Qing-Min Sun , Yu-Chen Shen , Qing-Shan Li , Yong-Jun Piao , Lin Gong

{"title":"Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells","authors":"Yang Jiao , Qing-Min Sun , Yu-Chen Shen , Qing-Shan Li , Yong-Jun Piao , Lin Gong","doi":"10.1016/j.acthis.2024.152184","DOIUrl":"10.1016/j.acthis.2024.152184","url":null,"abstract":"<div><h3>Background</h3><p>There is an urgent need for new treatments to solve hair loss problem. As mesenchymal stem cells were proved to have effects on promoting tissue repair and regeneration, in which the exosome plays a vital role, we aim to investigate the influence of umbilical cord mesenchymal stem cells exosome (UCMSC-Exos) on hair growth and its mechanism.</p></div><div><h3>Methods</h3><p>The hUCMSC-Exos were extracted by ultracentrifugation. Primary fibroblasts were cultured with or without hUCMSC-Exos and cell proliferation was evaluated by CCK-8 assay. C57BL/6 mice model of depilation-induced hair regrowth was treated with either hUCMSC-Exos (200 μg/mL) or PBS on one side of the dorsal back. Real time quantitative PCR, flow cytometry analysis, immunohistochemistry and Immunofluorescent staining were used to analyze the regulative effect of hUCMSC-Exos on hair follicle stem/progenitor cells and Wnt/β-catenin pathway.</p></div><div><h3>Results</h3><p>The proliferation of fibroblasts incubated with hUCMSC-Exos at the concentration of 200 μg/mL was greater than other groups. Treatment with hUCMSC-Exos resulted in rapid reentry into anagen. Hair follicle stem/progenitor cell markers (K15, Lgr5, Lgr6, CD34 and Lrig1) and Wnt/β-catenin pathway related factors (Wnt5, Lef1, Lrp5 and β-catenin) were increased in hUCMSC-Exos-injected region.</p></div><div><h3>Conclusion</h3><p>hUCMSC-Exos promote fibroblasts proliferation and accelerate mouse hair regrowth by upregulating hair follicle stem/progenitor cell and Wnt/β-catenin pathway, which suggests potential therapeutic approaches for hair loss disorders.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myeloid-derived suppressor cells: Implication in myeloid malignancies and immunotherapy 髓源性抑制细胞：髓源性抑制细胞：对髓系恶性肿瘤和免疫疗法的影响。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-18 DOI: 10.1016/j.acthis.2024.152183

Suncica Kapor , Milica Radojković , Juan F. Santibanez

引用次数: 0

Association of diabetes mellitus with risk of reproductive impairment in females: A comprehensive review 糖尿病与女性生殖障碍风险的关系：综述

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-14 DOI: 10.1016/j.acthis.2024.152173

Nida Andlib, Mohd Sajad, Sonu Chand Thakur

{"title":"Association of diabetes mellitus with risk of reproductive impairment in females: A comprehensive review","authors":"Nida Andlib, Mohd Sajad, Sonu Chand Thakur","doi":"10.1016/j.acthis.2024.152173","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152173","url":null,"abstract":"<div><p>Reproductive impairment is the most prevalent yet most ignored complication of diabetes mellitus. In diabetes, the problem associated with reproductive health is comprehensive in both males and females. Diabetic females have problems like delayed menarche, irregular menstrual cycle, subfertility, complications in pregnancy and early menopause. This may decrease reproductive age in diabetic females as the menarche is delayed and menopause is early in them. Like diabetic males, diabetic females also have the negative effect of oxidative stress on the reproductive system. This may lead to dysfunction of the ovary. It affects the physiological cycle like the ovary’s maturation, embryo development and pregnancy. These complications also affect the offspring, and they may also become diabetic. This review aims to concentrate on the effect of diabetes on the reproductive system of females and the impairment caused by it. We will also discuss in detail the role of the hypothalamus-pituitary ovary axis, diabetes impact on different reproductive phases of females, and the sexual disorders that occur in them.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141605122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artesunate inhibits vasculogenic mimicry in choroidal melanoma through HIF-1 α/ VEGF/PDGF pathway 青蒿琥酯通过HIF-1 α/ VEGF/PDGF途径抑制脉络膜黑色素瘤的血管生成模拟。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-07 DOI: 10.1016/j.acthis.2024.152174

Qing-yue Ma , Xiao-yan Xu , Yuan-zhang Zhu, Ning-ning Yao, Yi-chong Liu, Xiao-di Gao, Qian Zhang, Wen-juan Luo

引用次数: 0

The link between autophagy and psoriasis 自噬与牛皮癣之间的联系

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152166

Nannan Liang, Kaiming Zhang

引用次数: 0

Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum 参与大鼠胃窦中表达 P2X3 嘌呤受体的浆膜下传入神经末梢谷氨酸外排的蛋白质的免疫组织化学定位。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152170

Masato Hirakawa , Takuya Yokoyama , Sayed Sharif Abdali , Yoshio Yamamoto , Tomoyuki Saino

{"title":"Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum","authors":"Masato Hirakawa , Takuya Yokoyama , Sayed Sharif Abdali , Yoshio Yamamoto , Tomoyuki Saino","doi":"10.1016/j.acthis.2024.152170","DOIUrl":"10.1016/j.acthis.2024.152170","url":null,"abstract":"<div><p>We previously reported the presence of P2X3 purinoceptors (P2X3)-expressing subserosal afferent nerve endings consisting of net- and basket-like nerve endings in the rat gastric antrum. These nerve endings may morphologically be vagal mechanoreceptors activated by antral peristalsis. The present study investigated immunoreactivities for vesicular glutamate transporter (VGLUT) 1 and VGLUT2 as well as exocytosis-related proteins, i.e., core components of the SNARE complex (SNAP25, Stx1, and VAMP2) and synaptotagmin-1 (Syt1), in whole-mount preparations of the rat gastric antrum using double immunofluorescence. VGLUT1 immunoreactivity was not detected, whereas VGLUT2 immunoreactivity was observed in P2X3-immunoreactive subserosal nerve endings composed of both net- and basket-like endings. In net-like nerve endings, intense VGLUT2 immunoreactivity was localized in polygonal bulges of reticular nerve fibers and peripheral axon terminals. Furthermore, intense immunoreactivities for SNAP25, Stx1, and VAMP2 were localized in net-like nerve endings. Intense immunoreactivities for VAMP2 and Syt1 were observed in VGLUT2-immunoreactive net-like nerve endings. In basket-like nerve endings, VGLUT2 immunoreactivity was localized in pleomorphic terminal structures and small bulges surrounding the subserosal ganglion, whereas immunoreactivities for SNAP25, Stx1, and VAMP2 were weak in these nerve endings. VGLUT2-immunoreactive basket-like nerve endings were weakly immunoreactive for VAMP2 and Syt1. These results suggest that subserosal afferent nerve endings release glutamate by exocytosis mainly from net-like nerve endings to modulate their mechanoreceptor function.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000382/pdfft?md5=c74d10fb49258e2095898871d4428b78&pid=1-s2.0-S0065128124000382-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway” [Acta Histochem. 125(8) (2023) 152100] 通过血管生成素/Tie-2途径调节特发性肺纤维化中的血管生成"[《组织化学学报》(Acta Histochem.) 125(8) (2023) 152100]的更正。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152168

Yan Wu , Jun Zhang , Xintian Wang , Yuncong Xu , Jinxu Zheng

引用次数: 0

A comparison of teleost rodlet cells with apicomplexan cells 鱼类啮齿动物细胞与类囊体细胞的比较

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152167

Joseph A. DePasquale

引用次数: 0

A simple and economical method for unbiasedly quantifying the alveolar size of entire mouse lung tissue utilizing Hematoxylin and Eosin Staining 利用苏木精和伊红染色法对整个小鼠肺组织的肺泡大小进行无偏量化的简单经济方法

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152169

Chunyan Li , Xinru Zhang , Maolin Wang , Zhu Wang , Qi Cheng , Junrong Du , Tao Jia

引用次数: 0