Acta histochemica最新文献_第2页

Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells 从人脐带间充质干细胞中提取的外泌体刺激小鼠毛发再生。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-24 DOI: 10.1016/j.acthis.2024.152184

{"title":"Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells","authors":"","doi":"10.1016/j.acthis.2024.152184","DOIUrl":"10.1016/j.acthis.2024.152184","url":null,"abstract":"<div><h3>Background</h3><p>There is an urgent need for new treatments to solve hair loss problem. As mesenchymal stem cells were proved to have effects on promoting tissue repair and regeneration, in which the exosome plays a vital role, we aim to investigate the influence of umbilical cord mesenchymal stem cells exosome (UCMSC-Exos) on hair growth and its mechanism.</p></div><div><h3>Methods</h3><p>The hUCMSC-Exos were extracted by ultracentrifugation. Primary fibroblasts were cultured with or without hUCMSC-Exos and cell proliferation was evaluated by CCK-8 assay. C57BL/6 mice model of depilation-induced hair regrowth was treated with either hUCMSC-Exos (200 μg/mL) or PBS on one side of the dorsal back. Real time quantitative PCR, flow cytometry analysis, immunohistochemistry and Immunofluorescent staining were used to analyze the regulative effect of hUCMSC-Exos on hair follicle stem/progenitor cells and Wnt/β-catenin pathway.</p></div><div><h3>Results</h3><p>The proliferation of fibroblasts incubated with hUCMSC-Exos at the concentration of 200 μg/mL was greater than other groups. Treatment with hUCMSC-Exos resulted in rapid reentry into anagen. Hair follicle stem/progenitor cell markers (K15, Lgr5, Lgr6, CD34 and Lrig1) and Wnt/β-catenin pathway related factors (Wnt5, Lef1, Lrp5 and β-catenin) were increased in hUCMSC-Exos-injected region.</p></div><div><h3>Conclusion</h3><p>hUCMSC-Exos promote fibroblasts proliferation and accelerate mouse hair regrowth by upregulating hair follicle stem/progenitor cell and Wnt/β-catenin pathway, which suggests potential therapeutic approaches for hair loss disorders.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myeloid-derived suppressor cells: Implication in myeloid malignancies and immunotherapy 髓源性抑制细胞：髓源性抑制细胞：对髓系恶性肿瘤和免疫疗法的影响。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-18 DOI: 10.1016/j.acthis.2024.152183

引用次数: 0

Association of diabetes mellitus with risk of reproductive impairment in females: A comprehensive review 糖尿病与女性生殖障碍风险的关系：综述

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-14 DOI: 10.1016/j.acthis.2024.152173

Nida Andlib, Mohd Sajad, Sonu Chand Thakur

{"title":"Association of diabetes mellitus with risk of reproductive impairment in females: A comprehensive review","authors":"Nida Andlib, Mohd Sajad, Sonu Chand Thakur","doi":"10.1016/j.acthis.2024.152173","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152173","url":null,"abstract":"<div><p>Reproductive impairment is the most prevalent yet most ignored complication of diabetes mellitus. In diabetes, the problem associated with reproductive health is comprehensive in both males and females. Diabetic females have problems like delayed menarche, irregular menstrual cycle, subfertility, complications in pregnancy and early menopause. This may decrease reproductive age in diabetic females as the menarche is delayed and menopause is early in them. Like diabetic males, diabetic females also have the negative effect of oxidative stress on the reproductive system. This may lead to dysfunction of the ovary. It affects the physiological cycle like the ovary’s maturation, embryo development and pregnancy. These complications also affect the offspring, and they may also become diabetic. This review aims to concentrate on the effect of diabetes on the reproductive system of females and the impairment caused by it. We will also discuss in detail the role of the hypothalamus-pituitary ovary axis, diabetes impact on different reproductive phases of females, and the sexual disorders that occur in them.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141605122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artesunate inhibits vasculogenic mimicry in choroidal melanoma through HIF-1 α/ VEGF/PDGF pathway 青蒿琥酯通过HIF-1 α/ VEGF/PDGF途径抑制脉络膜黑色素瘤的血管生成模拟。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-07-07 DOI: 10.1016/j.acthis.2024.152174

Qing-yue Ma , Xiao-yan Xu , Yuan-zhang Zhu, Ning-ning Yao, Yi-chong Liu, Xiao-di Gao, Qian Zhang, Wen-juan Luo

引用次数: 0

The link between autophagy and psoriasis 自噬与牛皮癣之间的联系

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152166

Nannan Liang, Kaiming Zhang

引用次数: 0

Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum 参与大鼠胃窦中表达 P2X3 嘌呤受体的浆膜下传入神经末梢谷氨酸外排的蛋白质的免疫组织化学定位。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152170

Masato Hirakawa , Takuya Yokoyama , Sayed Sharif Abdali , Yoshio Yamamoto , Tomoyuki Saino

{"title":"Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum","authors":"Masato Hirakawa , Takuya Yokoyama , Sayed Sharif Abdali , Yoshio Yamamoto , Tomoyuki Saino","doi":"10.1016/j.acthis.2024.152170","DOIUrl":"10.1016/j.acthis.2024.152170","url":null,"abstract":"<div><p>We previously reported the presence of P2X3 purinoceptors (P2X3)-expressing subserosal afferent nerve endings consisting of net- and basket-like nerve endings in the rat gastric antrum. These nerve endings may morphologically be vagal mechanoreceptors activated by antral peristalsis. The present study investigated immunoreactivities for vesicular glutamate transporter (VGLUT) 1 and VGLUT2 as well as exocytosis-related proteins, i.e., core components of the SNARE complex (SNAP25, Stx1, and VAMP2) and synaptotagmin-1 (Syt1), in whole-mount preparations of the rat gastric antrum using double immunofluorescence. VGLUT1 immunoreactivity was not detected, whereas VGLUT2 immunoreactivity was observed in P2X3-immunoreactive subserosal nerve endings composed of both net- and basket-like endings. In net-like nerve endings, intense VGLUT2 immunoreactivity was localized in polygonal bulges of reticular nerve fibers and peripheral axon terminals. Furthermore, intense immunoreactivities for SNAP25, Stx1, and VAMP2 were localized in net-like nerve endings. Intense immunoreactivities for VAMP2 and Syt1 were observed in VGLUT2-immunoreactive net-like nerve endings. In basket-like nerve endings, VGLUT2 immunoreactivity was localized in pleomorphic terminal structures and small bulges surrounding the subserosal ganglion, whereas immunoreactivities for SNAP25, Stx1, and VAMP2 were weak in these nerve endings. VGLUT2-immunoreactive basket-like nerve endings were weakly immunoreactive for VAMP2 and Syt1. These results suggest that subserosal afferent nerve endings release glutamate by exocytosis mainly from net-like nerve endings to modulate their mechanoreceptor function.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000382/pdfft?md5=c74d10fb49258e2095898871d4428b78&pid=1-s2.0-S0065128124000382-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway” [Acta Histochem. 125(8) (2023) 152100] 通过血管生成素/Tie-2途径调节特发性肺纤维化中的血管生成"[《组织化学学报》(Acta Histochem.) 125(8) (2023) 152100]的更正。

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152168

Yan Wu , Jun Zhang , Xintian Wang , Yuncong Xu , Jinxu Zheng

引用次数: 0

A comparison of teleost rodlet cells with apicomplexan cells 鱼类啮齿动物细胞与类囊体细胞的比较

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152167

Joseph A. DePasquale

引用次数: 0

A simple and economical method for unbiasedly quantifying the alveolar size of entire mouse lung tissue utilizing Hematoxylin and Eosin Staining 利用苏木精和伊红染色法对整个小鼠肺组织的肺泡大小进行无偏量化的简单经济方法

IF 2.5 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152169

Chunyan Li , Xinru Zhang , Maolin Wang , Zhu Wang , Qi Cheng , Junrong Du , Tao Jia

引用次数: 0

Effects of sex differences on osteoarthritic changes after anterior cruciate ligament reconstruction in rats 性别差异对大鼠前十字韧带重建后骨关节炎变化的影响

IF 2.3 4区生物学

Acta histochemica Pub Date : 2024-05-01 DOI: 10.1016/j.acthis.2024.152172

Akinori Kaneguchi , Marina Kanehara , Kaoru Yamaoka , Takuya Umehara , Junya Ozawa

{"title":"Effects of sex differences on osteoarthritic changes after anterior cruciate ligament reconstruction in rats","authors":"Akinori Kaneguchi , Marina Kanehara , Kaoru Yamaoka , Takuya Umehara , Junya Ozawa","doi":"10.1016/j.acthis.2024.152172","DOIUrl":"10.1016/j.acthis.2024.152172","url":null,"abstract":"<div><p>The prevalence of primary osteoarthritis is higher in females than males. However, it remains unclear if there are sex differences in the incidence of post-traumatic osteoarthritis after anterior cruciate ligament (ACL) reconstruction. In this study, we aimed to investigate the effects of sex on osteoarthritic changes after ACL reconstruction using an animal model. Rats were divided into the following four groups: male control, male ACL reconstruction, female control, and female ACL reconstruction. ACL reconstruction surgery was performed on the right knees of rats in the ACL reconstruction groups, while rats in the control groups did not undergo knee surgery. At 1, 4, and 12 weeks after surgery, cartilage degeneration in the medial tibial plateau and osteophyte formation in the proximal tibia were histologically assessed. After ACL reconstruction, an increase in the Mankin score, cartilage fissures, and osteophyte formation were detected within 12 weeks in both male and female rats, with similar degrees of these changes between males and females. However, changes in cartilage thickness and chondrocyte density after ACL reconstruction differed between males and females. Cartilage thickening was observed in male rats but not in female rats. The increase in chondrocyte density in the anterior region was detected in both males and females but was more pronounced in female rats. In conclusion, osteoarthritic changes were observed after ACL reconstruction in both male and female rats, but differences in changes in cartilage thickness and chondrocyte density were observed between males and females.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0