Acta histochemica最新文献

{"title":"The link between autophagy and psoriasis","authors":"Nannan Liang,&nbsp;Kaiming Zhang","doi":"10.1016/j.acthis.2024.152166","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152166","url":null,"abstract":"<div><p>Autophagy is a lysosome-dependent, self-renewal mechanism that degrades and recycles cellular components in eukaryotic cells to maintain the homeostasis of the intracellular environment. Psoriasis is featured by increased inflammatory response, epidermal hyperproliferation and abnormal differentiation, infiltration of immune cells and increased expression levels of both endothelial adhesion molecules and angiogenic mediators. Evidence indicates that autophagy has important roles in many different types of cells, such as lymphocytes, keratinocytes, monocytes and mesenchymal stem cells (MSCs). This paper will review the role of autophagy in the pathogenesis of psoriasis and strategies for therapeutic modulation.</p><p>Key Message</p><p>Autophagy regulates the functions of cutaneous cells (MSCs, KCs, T cells and endothelial cells). Since reduced autophagy contributes in part to the pathogenesis of psoriasis, enhancement of autophagy can be an alternative approach to mitigate psoriasis.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152166"},"PeriodicalIF":2.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140813673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Saikosaponin-d regulates angiogenesis in idiopathic pulmonary fibrosis through angiopoietin/Tie-2 pathway” [Acta Histochem. 125(8) (2023) 152100]","authors":"Yan Wu ,&nbsp;Jun Zhang ,&nbsp;Xintian Wang ,&nbsp;Yuncong Xu ,&nbsp;Jinxu Zheng","doi":"10.1016/j.acthis.2024.152168","DOIUrl":"10.1016/j.acthis.2024.152168","url":null,"abstract":"","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152168"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000369/pdfft?md5=85ebd8252114cfed30311070175ce40e&pid=1-s2.0-S0065128124000369-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141320345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical localization of proteins involved in exocytosis of glutamate from P2X3 purinoceptor-expressing subserosal afferent nerve endings in the rat gastric antrum","authors":"Masato Hirakawa ,&nbsp;Takuya Yokoyama ,&nbsp;Sayed Sharif Abdali ,&nbsp;Yoshio Yamamoto ,&nbsp;Tomoyuki Saino","doi":"10.1016/j.acthis.2024.152170","DOIUrl":"10.1016/j.acthis.2024.152170","url":null,"abstract":"<div><p>We previously reported the presence of P2X3 purinoceptors (P2X3)-expressing subserosal afferent nerve endings consisting of net- and basket-like nerve endings in the rat gastric antrum. These nerve endings may morphologically be vagal mechanoreceptors activated by antral peristalsis. The present study investigated immunoreactivities for vesicular glutamate transporter (VGLUT) 1 and VGLUT2 as well as exocytosis-related proteins, i.e., core components of the SNARE complex (SNAP25, Stx1, and VAMP2) and synaptotagmin-1 (Syt1), in whole-mount preparations of the rat gastric antrum using double immunofluorescence. VGLUT1 immunoreactivity was not detected, whereas VGLUT2 immunoreactivity was observed in P2X3-immunoreactive subserosal nerve endings composed of both net- and basket-like endings. In net-like nerve endings, intense VGLUT2 immunoreactivity was localized in polygonal bulges of reticular nerve fibers and peripheral axon terminals. Furthermore, intense immunoreactivities for SNAP25, Stx1, and VAMP2 were localized in net-like nerve endings. Intense immunoreactivities for VAMP2 and Syt1 were observed in VGLUT2-immunoreactive net-like nerve endings. In basket-like nerve endings, VGLUT2 immunoreactivity was localized in pleomorphic terminal structures and small bulges surrounding the subserosal ganglion, whereas immunoreactivities for SNAP25, Stx1, and VAMP2 were weak in these nerve endings. VGLUT2-immunoreactive basket-like nerve endings were weakly immunoreactive for VAMP2 and Syt1. These results suggest that subserosal afferent nerve endings release glutamate by exocytosis mainly from net-like nerve endings to modulate their mechanoreceptor function.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152170"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000382/pdfft?md5=c74d10fb49258e2095898871d4428b78&pid=1-s2.0-S0065128124000382-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of teleost rodlet cells with apicomplexan cells","authors":"Joseph A. DePasquale","doi":"10.1016/j.acthis.2024.152167","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152167","url":null,"abstract":"<div><p>Rodlet cells are unique pear-shaped cells found primarily in the epithelium of the teleost fishes. The rodlet cell was first identified by Thèlohan in 1892 who named it <em>Rhabdospora thelohani</em> as it was believed to be a protozoan parasite of the phylum Apicomplexa. The rodlet cell as parasite paradigm persisted for several decades afterwards but has since faded in the last 20 years or so. The rodlet cell is now generally believed to be an immune cell, functioning as an early responder to parasite intrusion. This short review makes a detailed comparison of apicomplexan structure and behavior with that of the rodlet cell to further strengthen the argument against a parasitic nature for the fish cell. It is then proposed that apical microvilli of the rodlet cell serve as a mechanical trigger for rodlet discharge as possible defense against larger ectoparasites.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152167"},"PeriodicalIF":2.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple and economical method for unbiasedly quantifying the alveolar size of entire mouse lung tissue utilizing Hematoxylin and Eosin Staining","authors":"Chunyan Li ,&nbsp;Xinru Zhang ,&nbsp;Maolin Wang ,&nbsp;Zhu Wang ,&nbsp;Qi Cheng ,&nbsp;Junrong Du ,&nbsp;Tao Jia","doi":"10.1016/j.acthis.2024.152169","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152169","url":null,"abstract":"<div><p>Alveolar, the smallest structural and functional units within the respiratory system, play a crucial role in maintaining lung function. Alveolar damage is a typical pathological hallmark of respiratory diseases. Nevertheless, there is currently no simple, rapid, economical, and unbiased method for quantifying alveolar size for entire lung tissue. Here, firstly, we conducted lung sample slicing based on the size, shape, and distribution of airway branches of different lobes. Next, we performed HE staining on different slices. Then, we provided an unbiased quantification of alveolar size using free software ImageJ. Through this protocol, we demonstrated that C57Bl/6 mice exhibit varying alveolar sizes among different lobes. Collectively, we provided a simple and unbiased method for a more comprehensive quantification of alveolar size in mice, which holds promise for a broader range of respiratory research using mouse models.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152169"},"PeriodicalIF":2.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141291581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sex differences on osteoarthritic changes after anterior cruciate ligament reconstruction in rats","authors":"Akinori Kaneguchi ,&nbsp;Marina Kanehara ,&nbsp;Kaoru Yamaoka ,&nbsp;Takuya Umehara ,&nbsp;Junya Ozawa","doi":"10.1016/j.acthis.2024.152172","DOIUrl":"10.1016/j.acthis.2024.152172","url":null,"abstract":"<div><p>The prevalence of primary osteoarthritis is higher in females than males. However, it remains unclear if there are sex differences in the incidence of post-traumatic osteoarthritis after anterior cruciate ligament (ACL) reconstruction. In this study, we aimed to investigate the effects of sex on osteoarthritic changes after ACL reconstruction using an animal model. Rats were divided into the following four groups: male control, male ACL reconstruction, female control, and female ACL reconstruction. ACL reconstruction surgery was performed on the right knees of rats in the ACL reconstruction groups, while rats in the control groups did not undergo knee surgery. At 1, 4, and 12 weeks after surgery, cartilage degeneration in the medial tibial plateau and osteophyte formation in the proximal tibia were histologically assessed. After ACL reconstruction, an increase in the Mankin score, cartilage fissures, and osteophyte formation were detected within 12 weeks in both male and female rats, with similar degrees of these changes between males and females. However, changes in cartilage thickness and chondrocyte density after ACL reconstruction differed between males and females. Cartilage thickening was observed in male rats but not in female rats. The increase in chondrocyte density in the anterior region was detected in both males and females but was more pronounced in female rats. In conclusion, osteoarthritic changes were observed after ACL reconstruction in both male and female rats, but differences in changes in cartilage thickness and chondrocyte density were observed between males and females.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152172"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141465399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dihydroartemisinin breaks the immunosuppressive tumor niche during cisplatin treatment in Hepatocellular carcinoma","authors":"Yanguang Yang ,&nbsp;Yuting Gao ,&nbsp;Yi Gong ,&nbsp;Junlan Lu ,&nbsp;Shenghao Li ,&nbsp;Yajun Xiong ,&nbsp;Yuman Zhang ,&nbsp;Dan Wang ,&nbsp;Peng Gong ,&nbsp;Yunfeng Li ,&nbsp;Xinli Shi","doi":"10.1016/j.acthis.2024.152171","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152171","url":null,"abstract":"<div><h3>Objective</h3><p>Hepatocellular carcinoma, characterized by high mortality rates, often exhibits limited responsiveness to conventional treatments such as surgery, radiotherapy, and chemotherapy. Therefore, identifying a sensitizer for cisplatin has become crucial. Dihydroartemisinin, known for its potent role of tumor treatment, arises as a prospective candidate for cisplatin sensitization in clinical settings.</p></div><div><h3>Methods</h3><p>A mouse model of liver tumor was established through chemical induction of DEN/TCPOBOP. Upon successful model establishment, ultrasound was employed to detect tumors, Hematoxylin and eosin staining was conducted for observation of liver tissue pathology, and ELISA was utilized to assess cytokine changes (IFN-γ, IL-2, IL-4, IL-10, TGF-β, IL-1β, CCL2, and CCL21) in peripheral blood, para-tumor tissues, and tumor tissues. The infiltration of CD8⁺T cells and macrophages in tumor tissue sections was detected by immunofluorescence.</p></div><div><h3>Results</h3><p>Dihydroartemisinin combined with cisplatin obviously restrained the growth of liver tumors in mice and improved the weight and spleen loss caused by cisplatin. Cisplatin treatment of liver tumor mice increased the content of CCL2 and the number of macrophages in tumor tissues and promoted the formation of an immunosuppressive microenvironment. The combination therapy decreased the content of TGF-β in tumor tissues while increasing CCL2 levels in para-tumor tissues. Both combination therapy and cisplatin alone increased the number of CD8⁺T cells in tumor tissue, but there was no difference between them.</p></div><div><h3>Conclusion</h3><p>Dihydroartemisinin combined with cisplatin obviously prevented the deterioration of liver tumor in hepatocellular carcinoma mice and improve the therapeutic effect of cisplatin by improving the immunosuppressive microenvironment induced by cisplatin. Our findings provide a theoretical basis for considering dihydroartemisinin as an adjuvant drug for cisplatin in the treatment of hepatocellular carcinoma in the future.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 4","pages":"Article 152171"},"PeriodicalIF":2.3,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical localization and expression of heat shock proteins (HSP27, HSP60, HSP70, and HSP90) during the oestrous cycle, pregnancy, and lactation in rat ovaries","authors":"Banu Kandil ,&nbsp;Nevin Kurtdede ,&nbsp;Alev Gürol Bayraktaroglu","doi":"10.1016/j.acthis.2024.152157","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152157","url":null,"abstract":"<div><p>This study aimed to determine the expressions of HSP27, HSP60, HSP70, and HSP90 in rat ovaries during the oestrous cycle, pregnancy, and lactation. In follicle cells, HSP27 and HSP70 expression was not observed. HSP60 in oocytes was higher in the early stages of follicular development but decreased and disappeared as the follicle grew. HSP60 in granulosa and theca cells increased with follicle development and decreased with atresia. HSP90 in follicle cells did not change during follicle development or atresia. The expression of HSPs in interstitial cells was higher in the proestrus and estrus phases of the estrous cycle. The expression of HSPs in these cells was higher on day 5 of pregnancy, decreased on day 10, and decreased further on days 15 and 20. The expression of HSPs, which decreased in the second half of pregnancy, increased again on the first day of lactation. The expression of HSPs then decreased on day 5 of lactation and further decreased on days 10 and 20. HSP60 and HSP90 were positive in new and old corpus luteums (CLs) and their expression did not change during luteal development or regression. HSP27 and HSP70 were absent in new CLs. HSP27 was positive in old CLs and showed the same staining pattern during luteal regression. HSP70 expression was determined in old cyclic CLs during the oestrous cycle and pregnancy and decreased with luteal regression. HSP70 expression in old pregnancy CLs during lactation was very weak compared to the oestrous cycle and pregnancy. In conclusion, HSP60 and HSP90 may participate in folliculogenesis, luteal development, and steroidogenesis in luteal cells, and HSP27, HSP60, HSP70, and HSP90 may be effective in luteal regression and steroidogenesis in interstitial cells. HSP27 and HSP70 may be used as markers to identify old CLs in rats.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 3","pages":"Article 152157"},"PeriodicalIF":2.5,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140350151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropeptides in the rat claustrum – An immunohistochemical detection","authors":"Anna Lipiec-Borowicz ,&nbsp;Artur Pałasz ,&nbsp;Aleksandra Suszka-Świtek ,&nbsp;Łukasz Filipczyk ,&nbsp;Alessandra Della Vecchia ,&nbsp;John J. Worthington ,&nbsp;Aneta Piwowarczyk-Nowak","doi":"10.1016/j.acthis.2024.152156","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152156","url":null,"abstract":"<div><p>Neuropeptides are involved in numerous brain activities and are responsible for a wide spectrum of higher mental functions. The main purpose of this outline structural qualitative study was to identify the possible immunoreactivity of classical neuropeptides, as well as novel ones such as nesfatin-1, phoenixin (PNX), spexin (SPX), neuromedin U (NMU) and respective receptors within the rat claustrum for the first time. The study shows the novel identification of peptidergic neurotransmission in the rat claustrum which potentially implicates a contribution of this neuropeptide to numerous central neurosecretory mechanisms.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 3","pages":"Article 152156"},"PeriodicalIF":2.5,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140180435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sheet-like interstitial cells connect epithelial and smooth muscle cells in the mouse prostate","authors":"Yoshihiro Kawaguchi ,&nbsp;Keisuke Ohta ,&nbsp;Tasuku Hiroshige ,&nbsp;Kei-ichiro Uemura ,&nbsp;Akinobu Togo ,&nbsp;Kei-ichiro Nakamura ,&nbsp;Tsukasa Igawa","doi":"10.1016/j.acthis.2024.152153","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152153","url":null,"abstract":"<div><p>Epithelial−stromal relationship in the prostate gland is crucial for maintaining homeostasis, including functional differentiation, proliferation, and quiescence. Pathological stromal changes are believed to cause benign prostatic hyperplasia (BPH). The prostate stromal tissue is known to have several subtypes of interstitial cells that connect the epithelium and smooth muscle. However, the characteristics of their morphology and connection patterns are not fully understood. Therefore, we aimed to investigated the three-dimensional morphology and intercellular interactions of interstitial cells in the prostate ventral lobe of mature wild-type mice using immunohistochemistry and focused ion beam–scanning electron microscopy tomography (FIB–SEM tomography). The prostate interstitial cells exhibited immunohistochemical subtypes, including PDGFRα single-positive, CD34 single-positive, and CD34 and PDGFRα double-positive. PDGFRα single-positive cells were observed as elongated cells just below the epithelium, CD34 single-positive cells were observed as polygonal cells in the area away from the epithelium, and double-positive cells were observed as elongated cells situated slightly deeper than PDGFRα single-positive cells. Furthermore, connexin43-immunoreactive puncta were observed on interstitial cells just beneath the epithelium, suggestive of possible electrical connections among the PDGFRα single-positive interstitial cells. Three-dimensional structural analysis using FIB–SEM tomography revealed sheet-like multilayered interstitial cells that appear to separate the glandular terminal from the deeper interstitial tissue, which includes smooth muscle and capillaries. Further, epithelial cells might be indirectly connected to the smooth muscle and nerve fibers via these sheet-like multilayered interstitial cellular networks. These findings suggest that the cellular network that separates the glandular terminals from the deep interstitial tissue functionally bridges the epithelium and smooth muscle, possibly playing a pivotal role in prostate tissue homeostasis through the epithelial−smooth muscle or epithelial−stromal relationships.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":"126 3","pages":"Article 152153"},"PeriodicalIF":2.5,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000217/pdfft?md5=29f84e630c9a812073b56d284b3365cc&pid=1-s2.0-S0065128124000217-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140123149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}