Acta histochemica最新文献

{"title":"Immunolocalization patterns of histone-deacetylases in salivary glands of mice during postnatal development","authors":"Chubo Yang ,&nbsp;Xuejing Song ,&nbsp;Jiaqi Kong ,&nbsp;Huishu Li ,&nbsp;Yuanbo Zhan","doi":"10.1016/j.acthis.2024.152144","DOIUrl":"10.1016/j.acthis.2024.152144","url":null,"abstract":"<div><h3>Objective</h3><p>Histone-deacetylases (HDACs) are epigenetic modulators involved in the control of gene expression. No data are available on the expression or subcellular localization of HDACs in salivary glands. The present study aims to examine the subcellular distribution of HDACs in salivary glands during postnatal development.</p></div><div><h3>Design</h3><p>The major salivary glands of C57/BL6 mice were separately removed at 10, 25, 30,60 and 90 days after birth. Hematoxylin-eosin (H&amp;E) and immunohistochemical staining were performed for HDACs. Gene Expression of HDACs in C57BL/6. NOD-Aec1Aec2 mice salivary glands during the development of Sjögren's syndrome-like illness were also analyzed by using the gene expression datasets (GSE 15640).</p></div><div><h3>Results</h3><p>In the mice salivary gland, HDACs were found to have different localization patterns at various stages of development (10, 25, 30, 60, and 90 days). Apart from HDAC6, ductal cells of salivary glands were the primary sites for HDAC localization. HDAC2, 8, 5, 10 and 11 were expressed at high levels in the salivary gland after birth while HDAC6 showed no expression during postnatal development. This suggests that these HDAC subtypes may have different roles in salivary gland function. In the context of Sjögren's syndrome-like illness, HDAC 2, 8 and 10 showed low expression while HDAC1, 6,5,3 and 11 had relatively high expression in the salivary gland.</p></div><div><h3>Conclusions</h3><p>This study has provided an important reference for understanding the spatiotemporal-specific expression of HDACs in the salivary gland. These results offer new clues for the experimenters and hold promise for developing innovative therapeutic strategies for salivary gland-related diseases.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139926252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delineation of the healthy rabbit duodenum by immunohistochemistry – A short communication","authors":"Gabriella Meier Bürgisser ,&nbsp;Dorothea M. Heuberger ,&nbsp;Pietro Giovanoli ,&nbsp;Maurizio Calcagni ,&nbsp;Johanna Buschmann","doi":"10.1016/j.acthis.2024.152136","DOIUrl":"10.1016/j.acthis.2024.152136","url":null,"abstract":"<div><p>The duodenum acts as a vital organ that performs fundamental physiological functions like digestion and nutrient absorption. Situated in the lower abdomen, the duodenum is located between the stomach and the jejunum. Usually, the duodenum is divided into four anatomical portions. We here compare paraffin embedded and cryosections of the healthy rabbit duodenum for research purposes. This analysis evaluates the differential outcomes resulting from the application of these fixation methodologies in conjunction with immunohistochemical assays targeting extracellular matrix markers collagen I, collagen III, fibronectin, α-smooth muscle actin (α-SMA), and proliferation marker ki67 as well as inflammatory marker PAR-2. Subsequent recommendations are provided based on our findings. Furthermore, the advantage of an antigen retrieval step in immunohistochemical labelling of paraffin sections was demonstrated and confirmed with an isotype negative control. Basic classical histological stainings as HE, GT and elastin were also performed. Comparison of different stainings and labellings was performed in serial sections, showing that adjacent to the circular muscle of the duodenum, the connective tissue was composed of collagen I and fibronectin, while the artery and vein walls were predominantly α-SMA positive. Moreover, PAR-2 immunohistochemical staining was performed, where particularly a type of gland adjacent to Brunner’s glands showed prominent PAR-2 positive areas, while the Brunner’s glands themselves were PAR-2 negative. Proliferating ki67 positive cells facing the lumen were highly abundant in all kinds of glands except for the Brunner’s glands. This effort serves to furnish the research community with reference imagery pertinent to scientists opting for the rabbit duodenum model. The diversity of staining techniques employed herein establishes a foundational repository of images, primed for comparative analysis against pathological conditions. Furthermore, these images hold the potential to illustrate inter-species variations. For instance, they can be juxtaposed against murine or rat intestinal tracts, or even offer insights into the human context.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000047/pdfft?md5=77821ef30acc3ddbfed686452ca254ef&pid=1-s2.0-S0065128124000047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139648365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioncogenic roles of USP9Y and DDX3Y in lung cancer: USP9Y stabilizes DDX3Y by preventing its degradation through deubiquitination","authors":"Lei Xiu ,&nbsp;Bo Ma ,&nbsp;Lili Ding","doi":"10.1016/j.acthis.2023.152132","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152132","url":null,"abstract":"<div><p>In previous studies, downregulation of USP9Y and DDX3Y in lung cancer (LC) tissues was identified, while their function in LC progression remains elusive. In our current work, we intended to elucidate the effect and mechanisms of USP9Y and DDX3Y in LC. Gene downregulation has been confirmed in our LC tissues and cells. The effect of USP9Y or DDX3Y on LC cell malignancies was analyzed by functional assay. Both USP9Y and DDX3Y overexpression showed suppressive impact on LC cell malignancies. USP9Y overexpression has also been demonstrated to inhibit tumorigenesis in vivo. Based on GEPIA database, it was found that there was a positive correlation between the levels of USP9Y and DDX3Y in LC tissues. The mRNA expression of DDX3Y was not affected by USP9Y overexpression, while its protein levels were significantly up-regulated in USP9Y overexpressed LC cells. Moreover, USP9Y interacted with DDX3Y and has been demonstrated to stabilize DDX3Y expression by preventing its degradation via deubiquitination. In conclusion, USP9Y and DDX3Y exerted antioncogenic effects on the cell proliferation potential, cell cycle process, apoptosis, and tumorigenesis of LC. USP9Y binds to DDX3Y to prevent DDX3Y degradation through deubiquitination.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128123001393/pdfft?md5=5121f558eb23049a58fe34a424eb92e9&pid=1-s2.0-S0065128123001393-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Peroxisomal trans-2-enoyl-CoA inhibits proliferation, migration and invasion of hepatocellular carcinoma cells” [Acta Histochem. 125 (2023) 152002]","authors":"Qingqing Luo ,&nbsp;Liewang Qiu ,&nbsp;Ke Zhan ,&nbsp;Lu Zeng ,&nbsp;Shengtao Liao ,&nbsp;Chuanfei Li ,&nbsp;Zhechuan Mei ,&nbsp;Lin Lv","doi":"10.1016/j.acthis.2023.152130","DOIUrl":"10.1016/j.acthis.2023.152130","url":null,"abstract":"","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S006512812300137X/pdfft?md5=cfe0280d753cd5dbf24be83128b7dcf3&pid=1-s2.0-S006512812300137X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139097104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT1 regulates endoplasmic reticulum stress-related organ damage","authors":"He Hu ,&nbsp;Weichao Xu ,&nbsp;Yan Li ,&nbsp;Zhicheng Wang ,&nbsp;Siyue Wang ,&nbsp;Yansheng Liu ,&nbsp;Minan Bai ,&nbsp;Yingying Lou ,&nbsp;Qian Yang","doi":"10.1016/j.acthis.2024.152134","DOIUrl":"https://doi.org/10.1016/j.acthis.2024.152134","url":null,"abstract":"<div><p>Endoplasmic reticulum (ER) stress plays a key role in the pathogenesis of several organ damages. Studies show that excessive ER stress (ERS) can destroy cellular homeostasis, causing cell damage and physiological dysfunction in various organs. In recent years, Sirtuin1 (SIRT1) has become a research hotspot on ERS. Increasing evidence suggests that SIRT1 plays a positive role in various ERS-induced organ damage via multiple mechanisms, including inhibiting cellular apoptosis and promoting autophagy. SIRT1 can also alleviate liver, heart, lung, kidney, and intestinal damage by inhibiting ERS. We discuss the possible mechanism of SIRT1, explore potential therapeutic targets of diseases, and provide a theoretical basis for treating ERS-related diseases.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128124000023/pdfft?md5=561119be99ffbeaf95b6311f72cb7898&pid=1-s2.0-S0065128124000023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139487519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of FXN overexpression on ferroptosis in L-Glu-induced SH-SY5Y cells","authors":"Mengran Wang ,&nbsp;Tingting Xuan ,&nbsp;Haining Li ,&nbsp;Jing An ,&nbsp;Tianhui Hao ,&nbsp;Jiang Cheng","doi":"10.1016/j.acthis.2024.152135","DOIUrl":"10.1016/j.acthis.2024.152135","url":null,"abstract":"<div><h3>Background</h3><p><span>Alzheimer's disease (AD) is a complex, multifactorial neurodegenerative disease<span><span>. However, the pathogenesis remains unclear. Recently, an increasing number of studies have demonstrated that ferroptosis is a new type of iron-dependent </span>programmed cell death, contributes to the death of nerve cells in AD. By controlling </span></span>iron homeostasis<span> and mitochondrial function, the particular protein called frataxin<span> (FXN), which is situated in the mitochondrial matrix, is a critical regulator of ferroptosis disease. It is encoded by the nuclear gene FXN. Here, we identified a novel underlying mechanism through which ferroptosis mediated by FXN contributes to AD.</span></span></p></div><div><h3>Methods</h3><p><span><span><span>Human neuroblastoma cells (SH-SY5Y) were injured by L-glutamate (L-Glu). Overexpression of FXN by lentiviral transfection. In each experimental group, we assessed the </span>ultrastructure<span> of the mitochondria, the presence of iron and intracellular Fe2 + , the levels of reactive oxygen species<span>, the mitochondrial membrane potential (MMP), and lipid peroxidation. Quantification was done for </span></span></span>malondialdehyde<span><span> (MDA) and reduced glutathione<span> (GSH), as well as reactive oxygen species (ROS). Western blot and cellular </span></span>immunofluorescence assays<span> were used to detect the expression of xCT and GPX4 proteins which in System Xc-/GPX4 pathway, and the protein expressions<span> of ACSL4 and </span></span></span></span>TfR1 were investigated by Western blot.</p></div><div><h3>Results</h3><p><span><span>The present work showed: (1) The expression of FXN was reduced in the L-Glu group; (2) Compared with the Control group, MMP<span> was reduced in the L-Glu group, and mitochondria were observed to shrink and cristae were deformed, reduced or disappeared by </span></span>transmission electron microscopy<span>, and after FXN overexpression and ferrostatin-1 (Fer-1) (10 μmol/L) intervened, MMP was increased and mitochondrial morphology was significantly improved, suggesting that mitochondrial function was impaired in the L-Glu group, and overexpression of FXN could improve the manifestation of mitochondrial function impairment. (3) In the L-Glu group, ROS, MDA, iron ion concentration and Fe</span></span>²⁺<span> levels were increased, GSH was decreased. Elevated expression of ACSL4 and TfR1, important regulatory proteins<span> of ferroptosis, was detected by Western blot, and the expression of xCT and GPX4 in the System Xc-/GPX4 pathway was reduced by Western blot and cellular immunofluorescence. However, the above results were reversed when FXN overexpression and Fer-1 intervened.</span></span></p></div><div><h3>Conclusion</h3><p>To conclude, our research demonstrates that an elevated expression of FXN effectively demonstrates a robust neuroprotective effect against oxidative damage induced by L-Glu. Moreover, ","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139545165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1","authors":"Jiangxia Li ,&nbsp;Ke Xu ,&nbsp;Yunqing Cui ,&nbsp;Tianyuan Xu ,&nbsp;Wenchao Fei ,&nbsp;Cuiting Lyu ,&nbsp;Yinjue Yu ,&nbsp;Lina Yang ,&nbsp;Yang Hong ,&nbsp;Gong Yang","doi":"10.1016/j.acthis.2024.152133","DOIUrl":"10.1016/j.acthis.2024.152133","url":null,"abstract":"<div><p><span><span><span>Osteoporosis (OP) is a common disease among older adults. The promotion of </span>osteoblast differentiation plays a crucial role in alleviating OP symptoms. </span>Extracellular matrix protein<span><span> 1 (ECM1) has been reported to be closely associated with osteogenic differentiation. In this study, we constructed U2OS cell lines with ECM1 knockdown and ECM1a overexpression based on knockdown, and identified the target </span>miRNA<span><span><span> (miR-1260b) by sequencing. Overexpression of miR-1260b promoted the osteogenic differentiation of U2OS and MG63 cells, as demonstrated by increased alkaline phosphatase (ALP) activity, matrix </span>mineralization, and Runt-Related Transcription Factor 2 (RUNX2), </span>Osteopontin (OPN), Collagen I (COL1A1), and </span></span></span>Osteocalcin<span><span> (OCN) protein expressions, whereas low expression of miR-1260b had the opposite effect. In addition, miR-1260b expression was decreased in OP patients than in non-OP patients. Next, we predicted the target gene of miRNA through TargetScan and miRDB and found that miR-1260b negatively regulated </span>GDP dissociation inhibitor<span><span> 1 (GDI1) by directly binding to its 3′-untranslated region. Subsequent experiments revealed that GDI1 overexpression decreased ALP activity and calcium deposit, reduced RUNX2, OPN, COL1A1, and OCN expression levels, and reversed the effects of miR-1260b on osteogenic differentiation. In conclusion, ECM1-related miR-1260b promotes osteogenic differentiation by targeting GDI1 in U2OS and MG63 cells. Thus, this study has significant implication for osteoporosis </span>treatment.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139545162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Astrocyte S100β expression and selective differentiation to GFAP and GS in the entorhinal cortex during ageing in the 3xTg-Alzheimer's disease mouse model","authors":"J.J. Rodríguez ,&nbsp;E. Gardenal ,&nbsp;F. Zallo ,&nbsp;A. Arrue ,&nbsp;Joan Cabot ,&nbsp;X. Busquets","doi":"10.1016/j.acthis.2023.152131","DOIUrl":"10.1016/j.acthis.2023.152131","url":null,"abstract":"<div><p>The study of astrocytes and its role in the development and evolution of neurodegenerative diseases, including Alzheimer’s disease (AD) is essential to fully understand their aetiology. The aim if this study is to deepen into the concept of the heterogeneity of astrocyte subpopulations in the EC and in particular the identification of differentially functioning astrocyte subpopulations that respond differently to AD progression. S100β protein belongs to group of small calcium regulators of cell membrane channels and pumps that are expressed by astrocytes and is hypothesised to play and have a relevant role in AD development. We analysed the selective differentiation of S100β-positive astrocytes into Glutamine synthetase (GS) and Glial fibrillary acidic protein (GFAP)-positive sub-groups in the entorhinal cortex (EC) of AD triple transgenic animal model (3xTg-AD). EC is the brain region earliest affected in humans AD but also in this closest animal model regarding their pathology and time course. We observed no changes in the number of S100β-positive astrocytes between 1 and 18 months of age in the EC of 3xTg-AD mice. However, we identified relevant morphological changes in S100β/GFAP positive astrocytes showing a significant reduction in their surface and volume whilst an increase in number and percentage. Furthermore, the percentage of S100β/GS positive astrocyte population was also increased in 18 months old 3xTg-AD mice compared to the non-Tg mice. Our findings reveal the presence of differentially controlled astrocyte populations that respond differently to AD progression in the EC of 3xTg-AD mice. These results highpoints the major astrocytic role together with its early and marked affection in AD and arguing in favour of its importance in neurogenerative diseases and potential target for new therapeutic approaches.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128123001381/pdfft?md5=74a87340393f7b796d855ae2eb902a7c&pid=1-s2.0-S0065128123001381-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139063545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aflatoxin B1 and viruses’ combined pathogenesis: A mini systematics review of invitro and invivo studies","authors":"Mehdi Ahmadi ,&nbsp;Ramin Shahbahrami ,&nbsp;Fatemeh Khajeh ,&nbsp;Sepideh Khodaeivandi ,&nbsp;Ehsan Kakavandi ,&nbsp;Reza Hazrati Raziabad ,&nbsp;Kiandokht Ghanati","doi":"10.1016/j.acthis.2023.152116","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152116","url":null,"abstract":"<div><h3>Introduction</h3><p><span><span>The combined pathogenesis of Aflatoxin B1<span> (AFB1) and several viruses such as </span></span>HBV, </span>EBV and influenza virus have been investigated yet the molecular mechanism of their interaction and possible synergistic effects is not fully understood.</p></div><div><h3>Objectives</h3><p>The aim of the current systematic review<span> was to review in-vitro and in-vivo studies investigating the combined pathogenesis of aflatoxins and viruses.</span></p></div><div><h3>Methods</h3><p>This systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines. PECO (Population, Exposure, Comparator, and Outcome) criteria for invitro and invivo studies were used to evaluate the eligibility of the studies for systematic review.</p></div><div><h3>Results</h3><p>21 studies were eligible for qualitative analysis based on the inclusion criteria. Of all the included studies, 9 (42.9 %) were invivo, 7 (33.3 %) were invitro-invivo and 5(23.8) articles conducted only invitro assay. Furthermore 14 (66.6 %) article explored hepatitis B virus (HBV) combination with AFB1, 4 (19 %) studied influenza A virus (SIV), 2 (9.7 %) were about Epstein–Barr virus (EBV) and only 1 (4.7 %) included hepatitis C virus (HCV).</p></div><div><h3>Conclusion</h3><p>The limited collected evidence suggests that AFB1 enhanced EBV and influenza virus pathogenesis. AFB1 also operated as a cofactor for HBV and EBV-mediated carcinogenesis. On the other hand HBV and HCV also induced AFB-1 carcinogenesis. Due to the limited amount of included studies and the inconsistency of their results further studies especially on HBV and SIV are essential for better understanding of their combined mechanisms.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138678407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergies in stem cell research: Integrating technologies, strategies, and bionanomaterial innovations","authors":"Sharda Bharti,&nbsp;Awanish Kumar","doi":"10.1016/j.acthis.2023.152119","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152119","url":null,"abstract":"<div><p><span>Since the 1960 s, there has been a substantial amount of research directed towards investigating the biology of several types of stem cells, including embryonic stem cells, brain cells, and </span>mesenchymal stem cells<span>. In contemporary times, a wide array of stem cells has been utilized to treat several disorders, including bone marrow transplantation<span>. In recent years, stem cell treatment has developed as a very promising and advanced field of scientific research. The progress of therapeutic methodologies has resulted in significant amounts of anticipation and expectation. Recently, there has been a notable proliferation of experimental methodologies aimed at isolating and developing stem cells, which have emerged concurrently. Stem cells possess significant vitality and exhibit vigorous proliferation, making them suitable candidates for in vitro modification. This article examines the progress made in stem cell isolation and explores several methodologies employed to promote the differentiation of stem cells. This study also explores the method of isolating bio-nanomaterials and discusses their viewpoint in the context of stem cell research. It also covers the potential for investigating stem cell applications in bioprinting and the usage of bionanomaterial in stem cell-related technologies and research. In conclusion, the review article concludes by highlighting the importance of incorporating state-of-the-art methods and technological breakthroughs into the future of stem cell research. Putting such an emphasis on constant innovation highlights the ever-changing character of science and the never-ending drive toward unlocking the maximum therapeutic potential of stem cells. This review would be a useful resource for researchers, clinicians, and policymakers in the stem cell research area, guiding the next steps in this fast-developing scientific concern.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}