Acta histochemica最新文献

{"title":"Epidermal and dermal cells from adult rat eccrine sweat gland-containing skin can reconstruct the three-dimensional structure of eccrine sweat glands","authors":"Cangyu Wang ,&nbsp;Manxiu Cao ,&nbsp;Junhong Zhao ,&nbsp;Anqi Hu ,&nbsp;Xiang Liu ,&nbsp;Zihua Chen ,&nbsp;Cuiping Zhang ,&nbsp;Haihong Li","doi":"10.1016/j.acthis.2023.152120","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152120","url":null,"abstract":"<div><h3>Background</h3><p>Previously, we have demonstrated that eccrine sweat gland cells (ESGCs) can reconstruct the three-dimensional (3D) structure of eccrine sweat glands (ESGs). However, there is still a need to explore source cells capable of regenerating ESG to address the issue of ESG regeneration in ESGC-deficient conditions, such as severe burns.</p></div><div><h3>Methods</h3><p><span>The epidermal cells and dermal cells in adult rat ventral foot skin (ESG-bearing) were isolated. The isolated single epidermal cells and dermal cells were mixed with Matrigel, and then the mixture was implanted into the axillary/inguinal fat pads of </span>nude mice<span>. Five weeks after implantation, the Matrigel plugs were harvested and the morphology and differentiation of the cells were examined by H&amp;E staining and fluorescent immunohistochemical staining for ESG markers, such as Na+ -K+ -2Cl- cotransporter 1 (NKCC1), Na+ -K+ -ATPase (NKA), Foxa1 and K14.</span></p></div><div><h3>Results</h3><p>The epidermal cells and dermal cells of adult rat ventral foot skin can reconstruct 3D structure and express specific markers of ESGs in skin, such as NKCC1, NKA and Foxa1, indicating the ESG-phenotypic differentiation of the 3D structures. Double immunofluorescence<span> staining showed that some 3D structures expressed both the myoepithelial cell marker alpha-SMA and the common marker K14 of duct cells and myoepithelial cells, while some 3D structures expressed only K14, indicating that ESG-like 3D structures differentiated into duct-like and secretory coiled cells.</span></p></div><div><h3>Conclusion</h3><p>Epidermal and dermal cells from adult ESG-bearing skin can be used as a cell source for ESG regeneration.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138466871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delineation of the healthy rabbit tongue by immunohistochemistry – A technical note","authors":"Gabriella Meier Bürgisser ,&nbsp;Dorothea M. Heuberger ,&nbsp;Julia Rieber ,&nbsp;Iris Miescher ,&nbsp;Pietro Giovanoli ,&nbsp;Maurizio Calcagni ,&nbsp;Johanna Buschmann","doi":"10.1016/j.acthis.2023.152127","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152127","url":null,"abstract":"<div><p>In the oral cavity the tongue is an important muscular organ that supports the swallowing of food and liquids. It is responsible for the sense of taste, based on the many different taste buds it contains. Research in the field of tongue diseases demands for suitable preclinical models. The healthy rabbit tongue may therefore serve as baseline and reference for the pathological situation. With this consideration, we covered the fixation and histological stainings as well as the immunohistochemical labelling of the healthy rabbit tongue. In this technical note, initial choice of the fixative is discussed, with a comparison of formalin fixation and subsequent paraffin embedding versus cryopreservation. Moreover, we delineate the effect of an antigen retrieval step for formalin fixation by several examples. Finally, we provide ECM markers collagen I, collagen III, fibronectin, α-SMA and elastin staining as well as ki67 for proliferative status and PAR-2 protein expression as a marker for inflammatory status and nociception in tongue sections, mainly from the tongue body. Technically, we found superiority of paraffin sections for collagen I, collagen III, fibronectin, ki67 and α-SMA labelling, for selected detections systems. As for ECM components, the lamina propria was very rich in collagen and fibronectin, while the muscular body of the tongue showed only collagen and fibronectin positive areas between the muscle fibers. Moreover, α-SMA was clearly expressed in the walls of arteries and veins. The inflammatory marker PAR-2 on the other hand was prominently expressed in the salivary glands and to some extent in the walls of the vessels. Particular PAR-2 expression was found in the excretory ducts of the tongue. This technical note has the aim to provide baseline images that can be used to compare the pathological state of the diseased rabbit tongue as well as for inter-species comparison, such as mouse or rat tongue. Finally, it can be used for the comparison with the human situation.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0065128123001344/pdfft?md5=c30344e7d79fc265d68c34a06b2f56a1&pid=1-s2.0-S0065128123001344-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138466869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalpol antagonizes LPS-mediated inflammation and promotes osteoblast differentiation through the miR-124-3p/DNMT3b/TRAF6 axis","authors":"Pan Zhang,&nbsp;Qun Feng,&nbsp;Wenxiao Chen,&nbsp;Xizhuang Bai","doi":"10.1016/j.acthis.2023.152118","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152118","url":null,"abstract":"<div><h3>Background</h3><p><span>Dysregulated inflammation and osteoblast<span> differentiation are implicated in osteoporosis. Exploring the activity of </span></span>catalpol in inflammation and osteoblast differentiation deepens the understanding of osteoporosis pathogenesis.</p></div><div><h3>Methods</h3><p>LPS<span><span><span><span> was used to treated hFOB1.19 cells to induce inflammation and repress osteoblast differentiation. FOB1.19 cells were induced in osteoblast differentiation medium and treated with LPS and catalpol. Cell viability<span> was assessed using CCK-8. ALP and </span></span>Alizarin red S<span><span> staining were conducted for analyzing osteoblast differentiation. The levels of IL-1β, TNF-α and IL-6 were examined by ELISA. The </span>methylation<span> of TRAF6 promoter was examined through MS-PCR. The binding of miR-124–3p to </span></span></span>DNMT3b<span> and DNMT3b to TRAF6 promoter was determined with dual luciferase reporter and </span></span>ChIP assays.</span></p></div><div><h3>Results</h3><p>LPS enhanced secretion of inflammatory cytokines and suppressed osteoblast differentiation. MiR-124–3p and TRAF6 were upregulated and DNMT3b was downregulated in LPS-induced hFOB1.19 cells. Catalpol protected hFOB1.19 cells against LPS via inhibiting inflammation and promoting osteoblast differentiation. MiR-124–3p targeted DNMT3b, and its overexpression abrogated catalpol-mediated protection in LPS-treated hFOB1.19 cells. In addition, DNMT3b methylated TRAF6 promoter to restrain its expression. Catalpol exerted protective effects through suppression of the miR-124–3p/DNMT3b/TRAF6 axis in hFOB1.19 cells.</p></div><div><h3>Conclusion</h3><p>Catalpol antagonizes LPS-mediated inflammation and suppressive osteoblast differentiation via controlling the miR-124–3p/DNMT3b/TRAF6 axis.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138466870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BrdU does not induce hepatocellular damage in experimental Wistar rats","authors":"Abril Alondra Barrientos-Bonilla ,&nbsp;Paola Belem Pensado-Guevara ,&nbsp;Abraham Puga-Olguín ,&nbsp;Rasajna Nadella ,&nbsp;Aurora del Carmen Sánchez-García ,&nbsp;Laura Mireya Zavala-Flores ,&nbsp;Arnulfo Villanueva-Olivo ,&nbsp;Iliana Tamara Cibrián-Llanderal ,&nbsp;María de Jesús Rovirosa-Hernández ,&nbsp;Daniel Hernandez-Baltazar","doi":"10.1016/j.acthis.2023.152117","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152117","url":null,"abstract":"<div><p><span>Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and </span>neurogenesis<span><span><span>. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar </span>rats<span><span> in 30 %-partial hepatectomy (PHx) model. The rats received BrdU 50 mg/Kg by intraperitoneal injection at 0.5, 1, 2, 3, 6, 9 and 16 days after 30 %-PH. The rats were distributed into four groups as follows, control, sham, PHx/BrdU(-) and PHx/BrdU(+). On day 16, we evaluated hepatocellular nuclei and analyzed histopathological features by haematoxylin-eosin stain and apoptotic profile was qualified by caspase-3 presence. The systemic effect was evaluated by liver markers such as alanine<span> transferase (ALT), aspartate aminotransferase (AST), </span></span>lactate dehydrogenase<span> (LDH), alkaline phosphatase (AP), </span></span></span>bilirubin<span><span>, total proteins and serum albumin content. The statistical analysis consisted of a student t-test and one-way ANOVA. BrdU did not induce </span>apoptosis<span> or hepatocellular damage<span> in male rats. Multiple administrations of BrdU in male rats did not induce significant decrease body weight, but increased serum ALT and LDH levels were found. Our results show that the BrdU does not produce hepatocellular damage.</span></span></span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138439541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and cytochemical analysis of the brain under conditions of hypobaric hypoxia-induced oxygen deficiency in albino rats","authors":"Ruzanna Shushanyan,&nbsp;Anna Grigoryan,&nbsp;Tamara Abgaryan,&nbsp;Anna Karapetyan","doi":"10.1016/j.acthis.2023.152114","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152114","url":null,"abstract":"<div><p><span><span><span>High altitude sickness is a life-threatening disease that occurs among acclimatized individuals working or living at a high altitude accompanied by hypobaric </span>hypoxia<span> exposure. The prolonged influence of hypobaric hypoxia on the brain may trigger neuronal damage and cell death due to an oxygen deficiency. The purpose of the current study was to investigate the histomorphological changes in the </span></span>hippocampus<span><span>, cerebral cortex, </span>cerebellar cortex, and striatum of the rat’s brain following chronic hypobaric hypoxia. Fourteen </span></span>albino rats<span><span> were used for this investigation. The animals were exposed to chronic hypobaric hypoxia in the special decompression chamber at an altitude of 7000 m for 7 days. The histological analysis was conducted via </span>toluidine<span><span><span> staining and silver impregnation. DNA damage and cell apoptosis were assessed via </span>Feulgen staining. The histochemical assessment revealed increased dark neurons in the hippocampus with cell swelling. Silver impregnation showed increased argyrophilic neurons in the cerebellar cortex, striatum, CA1 subfield of the hippocampus, and cerebral cortex. The cytochemical analysis determined the increased apoptotic cells with hyperchromatic condensation and </span>pyknosis in the hippocampus subfields and cerebral cortex. In addition, it has been observed that hypoxia has resulted in small hemorrhages and perivascular edema within the cerebellar and cerebral cortex.</span></span></p><p>The results indicate brain injury observed in the various parts of the brain towards hypobaric hypoxia, however, the hippocampus showed greater vulnerability against hypoxic exposure in comparison to the striatum, cerebellum, and cerebral cortex. These changes support our insights regarding brain intolerance under conditions of hypoxia-induced oxygen deficiency and its histomorphological manifestations.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136697190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between neuropeptides and mucins in Crohn’s disease mucous cells","authors":"Anthea Miller ,&nbsp;Giuseppina Cutroneo ,&nbsp;Giorgia Pia Lombardo ,&nbsp;Roberta D’Angelo ,&nbsp;Socrate Pallio ,&nbsp;Alba Migliorato ,&nbsp;Angelo Fumia ,&nbsp;Angelo Favaloro ,&nbsp;Eugenia Rita Lauriano ,&nbsp;Simona Pergolizzi","doi":"10.1016/j.acthis.2023.152115","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152115","url":null,"abstract":"<div><p><span>Crohn’s disease (CD) and ulcerative colitis<span><span><span> (UC) are both inflammatory bowel diseases (IBD). Unlike UC, which is limited to the mucosa of the colon, CD inflammation is characterized by chronic mucosal </span>ulcerations<span> affecting the entire gastrointestinal tract. </span></span>Goblet cells (GCs) can be found in some lining epithelia, particularly in the respiratory and digestive tracts. GCs represent the main source of mucin that are the significant components of the </span></span>mucus<span><span><span> layer; hypertrophy of GCs and an increase in mucin production are observed in many enteric infections<span>. The cytoplasm of goblet cells may also contain neuropeptides, such as serotonin, that can be altered in inflammatory bowel disease (IBD). The defense system of the gut is represented by the </span></span>intestinal mucosal barrier<span>, its protective function is strictly connected to the regulation of the mucus layer and the coordination of the neuro-immune response. Paraformaldehyde-fixed intestinal tissues, obtained from fifteen patients with Crohn’s disease, were analyzed by immunostaining for </span></span>MUC2<span><span>, MUC4<span>, 5-HT, and VAChT. This study aims to define the link between neuropeptides and mucins in </span></span>mucous cells and their involvement in the inflammation process. Our results showed in mucous cells of Crohn’s disease (CD) patients a high expression of MUC4 and a decrease in the expression of vesicular acetylcholine transporter (VAChT) demonstrating the presence of an inflammatory state.</span></span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134832438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue","authors":"Lorenzo Alibardi","doi":"10.1016/j.acthis.2023.152113","DOIUrl":"10.1016/j.acthis.2023.152113","url":null,"abstract":"<div><h3>Purpose</h3><p>Lizard regeneration derives from the re-activation of a number of developmental genes after tail amputation. Among genes with the highest expression, as indicated from the transcriptome, is <em>lix1</em> which functional role is not known.</p></div><div><h3>Method</h3><p>An antibody that cross-reacts with the lizard <em>Podarcis muralis</em> lix1 has been utilized to detect by immunofluorescence the sites of localization of the protein in the regenerating tail.</p></div><div><h3>Results</h3><p>Lix1-protein is almost exclusively localized in the regenerating spinal cord (ependyma) and nerves growing into the blastema, in sparse blastema cells but is undetectable in other tissues.</p></div><div><h3>Conclusions</h3><p>Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72207930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats","authors":"Laís Bitencourt Guimarães ,&nbsp;Daniel Portela Dias Machado ,&nbsp;Beatriz Ferreira Carvalho Versiani Caldeira ,&nbsp;Larissa Tiemi Matuzake Vieira ,&nbsp;Gabriela Alves Santos ,&nbsp;Fabiana Rocha Araújo ,&nbsp;Leonardo Teotônio Machado ,&nbsp;Dawidson Assis Gomes ,&nbsp;Natália de Melo Ocarino ,&nbsp;Rogéria Serakides ,&nbsp;Amanda Maria Sena Reis","doi":"10.1016/j.acthis.2023.152112","DOIUrl":"10.1016/j.acthis.2023.152112","url":null,"abstract":"<div><p>Kisspeptin (Kp-10) is a neuropeptide that binds to GPR54 receptors, exerting several functions mainly in the nervous and reproductive systems of the body. However, its effects and mechanisms of action on the skeletal system remain poorly understood. This study evaluated the effects of different concentrations of Kp-10 on <em>in vitr</em>o osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established <em>in vitro</em>: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synthesis, percentage of area covered by MSCs/field and mineralized nodules/field, and immunocytochemistry of the GPR54 receptor tests. Furthermore, evaluation of gene transcripts for type I collagen, <em>Runx-2</em>, Bmp-2<em>,</em> bone sialoprotein, osteocalcin and osteopontin was performed using real-time RT-qPCR. It was observed that MSCs expressed GPR54 receptor to which Kp-10 binds during osteogenic differentiation, promoting a negative effect on osteogenic differentiation. This effect was observed at all the Kp-10 concentrations in a concentration-dependent manner, characterized by a decrease in the activity of alkaline phosphatase, collagen synthesis, mineralized nodules, and decreased expression of gene transcripts for type I collagen, osteocalcin, osteopontin, and <em>Runx-2</em>. Thus, Kp-10 inhibits <em>in vitro</em> osteogenic differentiation of MSCs extracted from the BM of adult Wistar rats.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72207931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro validation: GLY alleviates UV-induced corneal epithelial damage through the HMGB1-TLR/MyD88-NF-κB signaling pathway","authors":"XinYi Chen ,&nbsp;XiaoXiao Zheng ,&nbsp;Ting Shen ,&nbsp;Ting He ,&nbsp;YangQi Zhao ,&nbsp;Yi Dong","doi":"10.1016/j.acthis.2023.152111","DOIUrl":"10.1016/j.acthis.2023.152111","url":null,"abstract":"<div><p>UV-induced corneal damage is a common ocular surface injury that usually leads to corneal lesions causing persistent inflammation. High mobility group box 1 (HMGB1) is identified as an inflammatory alarm in various tissue injuries. Here, this study first evaluates the repair effect of the HMGB1-selective inhibitor GLY in UV-induced corneal damage; Secondly, the inhibitory effect of GLY on UV-induced corneal damage induced inflammation and the potential therapeutic mechanism of GLY were studied. GLY effectively attenuates the expression of UV-induced inflammatory factors and HMGB1, TLR/MyD88, NF-κB signaling pathway genes at the mRNA and protein levels. In addition, RT-PCR and Western Blot experiments after knocking down HMGB1 and TLR2/9 genes showed that GLY alleviated corneal inflammation by inhibiting the HMGB1-TLR/MyD88 signaling pathway. The results of this study show that targeting HMGB1-NF-κB by GLY can alleviate the inflammatory response induced by UV induction.</p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71520158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect on m6A methylation writer complex by the reduced MATR3 in pterygium","authors":"Qianqian Guo,&nbsp;Shichao Han","doi":"10.1016/j.acthis.2023.152101","DOIUrl":"https://doi.org/10.1016/j.acthis.2023.152101","url":null,"abstract":"<div><p><span><span>Pterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, </span>RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and </span><em>Matr3</em><span><span><span> as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3 in control but not pterygium was identified by mass spectrum. Immunoprecipitation was performed to validate the interaction between MATR3 and WTAP/METTL3/METTL14 complex. (Methylated) </span>RNA<span> immunoprecipitation was performed to further reveal that the binding affinity of WTAP and MATR3 was lost at 3′ UTR of RNA molecules of down-regulated genes in pterygium. Overall, we figured out the loss of intercrossing between MATR3 and N6-methyladenosine </span></span>methyltransferase complex, as well as indicated the potential impact on transcription of target genes in pterygium.</span></p></div>","PeriodicalId":6961,"journal":{"name":"Acta histochemica","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91991208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}