Comparison of collagen I and collagen III immunohistochemistry with Herovici staining in various rabbit organs

IF 2.3 4区生物学 Q4 CELL BIOLOGY

Acta histochemica Pub Date : 2025-04-18 DOI:10.1016/j.acthis.2025.152256

Gabriella Meier Bürgisser, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann

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Abstract

Collagen I and III distribution is not only crucial to assess the status of healing wounds, but also to characterise healthy connective tissue and pathological extracellular matrix composition. In this technical note, we have therefore compared the dual-coloured Herovici staining, indicating pink collagen I and blue collagen III in serial sections with immunohistochemistry (IHC) labellings for collagen I and III, respectively. Furthermore, we used, chromogenic DAB for IHC labelling. Seven different organs of a healthy New Zealand white rabbit were collected for this purpose, including kidney, liver, tonsil, tongue, duodenum, heart, and brain, respectively. A dual-coloured staining like Herovici turned out to be as good as two single-colour labellings utilising IHC. In some cases, co-localisation and extent of collagen I and III expression could be qualitatively visualised better using Herovici, with gradients of blue-violet-pink, than by mere comparison of labelling intensities side by side in two different sections, although taken at the same place as serial sections. Nevertheless, a quantitative analysis of the Collagen I-to-III ratio revealed no significant differences between these two approaches to assess the extracellular matrix composition. From these comparisons, we conclude that a Herovici staining is recommended as a valuable alternative staining to collagen I and III IHC; and it may act as a fast and cheap preliminary staining method. These findings encourage researchers focusing on ECM composition of the experimental rabbit tissue to use Herovici staining to determine the ratio of the extracellular collagen I and III expression.

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兔各脏器ⅰ型和ⅲ型胶原免疫组化与Herovici染色的比较

胶原I和III的分布不仅对评估伤口愈合状况至关重要，而且对表征健康结缔组织和病理细胞外基质组成也至关重要。因此，在本技术说明中，我们比较了双色Herovici染色，分别在免疫组织化学（IHC）标记的胶原I和III的连续切片中显示粉红色的胶原I和蓝色的胶原III。此外，我们使用显色DAB进行IHC标记。为此，我们收集了一只健康新西兰大白兔的7个不同器官，分别是肾脏、肝脏、扁桃体、舌头、十二指肠、心脏和大脑。像Herovici这样的双色染色结果与使用IHC的两个单色标签一样好。在某些情况下，使用蓝-紫-粉色梯度的Herovici可以更好地定性地观察I和III型胶原蛋白的共定位和表达程度，而不仅仅是在两个不同切片中并排比较标记强度，尽管作为连续切片在同一位置进行。然而，对胶原I-to-III比率的定量分析显示，这两种评估细胞外基质组成的方法之间没有显著差异。从这些比较中，我们得出结论，Herovici染色被推荐为一种有价值的替代胶原I和III IHC染色的方法；它可以作为一种快速、廉价的初步染色方法。这些发现鼓励研究人员关注实验兔组织的ECM组成，使用Herovici染色来确定细胞外胶原I和III的表达比例。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted