海军军医大学学报最新文献_第7页

Cryopreserved platelet-rich plasma enhancing calvarial bone defect repair in rabbits: an observation of outcome 冻存富血小板血浆增强兔颅骨骨缺损修复:结果观察

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00391

Jun Chen, Yun-fu Zhao, Qing-fu Zhang, Guo-qin Liu, Man-jiao Liu, Tian Yao

引用次数: 0

Solifenacin succinate in improving bladder irritative symptoms after transurethral resection of bladder tumors 琥珀酸索利那新改善经尿道膀胱肿瘤切除术后膀胱刺激症状

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00447

Lei Li, Z. Cao, X. Chuanliang, Rongchao Wei, Zhensheng Zhang, Yinghao Sun

引用次数: 0

Exercise and metabolic syndrome 运动与代谢综合征

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00434

Qing Gu, Zhi-Min Liu

引用次数: 0

Heat shock protein A12B decreases lipopolysaccharide-induced permeability of human umbilical vein endothelial cells 热休克蛋白A12B降低脂多糖诱导的人脐静脉内皮细胞通透性

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00483

Qiuxiang Kang, Yi Chen, Guifang Yu, Xu Zhang, K. Zhu

{"title":"Heat shock protein A12B decreases lipopolysaccharide-induced permeability of human umbilical vein endothelial cells","authors":"Qiuxiang Kang, Yi Chen, Guifang Yu, Xu Zhang, K. Zhu","doi":"10.3724/SP.J.1008.2015.00483","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00483","url":null,"abstract":"Objective To explore the lipopolysaccharide(LPS)-induced changes of heat shock protein A12B(HSPA12B)expression and its effect on the permeability of human umbilical vein endothelial cells(HUVECs).Methods The HUVECs cultured in vitro were divided into four groups: control group(without any treatment); LPS group(1 μg / m L LPS); LPS + p IRES2-EGFPHSPA12B-3Flag group(The HSPA12 B gene overexpression plasmid was transiently transfected into HUVECs and then LPS of 1 μg / m L was added); and LPS + p IRES2-EGFP-3Flag group(The negative control plasmid was transiently transfected into HUVECs and then LPS of 1 μg / m L was added).The transendothelial electrical resistance(TEER) of HUVECs was measured by MERSST × 01 Electrode.The expression of VE-cadherin was studied by flow cytometry.RT-PCR and Western blotting analysis were used to examine the expression changes of HSPA12 B mRNA and protein in HUVECs stimulated with LPS at various time points.Results It was showed that after stimulated with LPS,HSPA12 B mRNA and protein were gradually upregulated,and peaked at 12 h.HSPA12 B significantly increased the TEER value of HUVECs(P 0.001),and it could completely offset the decline of TEER value induced by LPS(P 0.001).Besides,HSPA12 B could cause upregulation of VE-cadherin expression.Conclusion HSPA12 B can reduce the permeability of vascular endothelial cells by up-regulating the expression of VE-cadherin,thus protecting the vascular endothelial barrier function of endothelial cells.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"483"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69847577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of α7 nicotinic acetylcholine receptor alleviates cerebral cortical neuron injury induced by oxygen-glucose deprivation α - 7烟碱乙酰胆碱受体的激活可减轻氧-葡萄糖剥夺所致的大脑皮质神经元损伤

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00472

Ru-juan Xin, Ting Zhao, Min Ni, Dong-Jie Li, F. Shen

{"title":"Activation of α7 nicotinic acetylcholine receptor alleviates cerebral cortical neuron injury induced by oxygen-glucose deprivation","authors":"Ru-juan Xin, Ting Zhao, Min Ni, Dong-Jie Li, F. Shen","doi":"10.3724/SP.J.1008.2015.00472","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00472","url":null,"abstract":"Objective To investigate the effect of activating α7 nicotinic acetylcholine receptor(α7n AchR) on cerebral cortical neurons injury induced by oxygen-glucose deprivation(OGD) and the possible mechanism.Methods Cerebral cortical neurons cultured for 7 d were randomly divided into three groups: control group,OGD group(Cells experienced a 12 h oxygen-glucose deprivation) and OGD group treated with PNU-282987(Cells experienced a 12 h oxygen-glucose deprivation with PNU-282987 pretreatment for 24 h).Cell viability was determined by CCK-8 assay,lactate dehydrogenase(LDH) was examined to reflect cell injury,apoptosis and reactive oxygen species(ROS) production were analyzed by flow cytometry,and expression of hemeoxygenase-1(HO-1) and hypoxia inducible factor-1α(HIF-1α) were detected by Western blotting analysis.Results OGD resulted in cell death,LDH increase,and cell apoptosis.Compared with the OGD group,PNU-282987 pretreated group had significantly increased cell survival(P 0.05),significantly decreased LDH level and ROS production(P 0.05),and significantly inhibited cell apoptosis(P 0.05).Meanwhile,HO-1 protein expression was significantly increased and HIF-1α protein expression was significantly reduced in PNU-282987 pretreated group compared with the OGD group(P 0.05).Conclusion Activation of α7n AchR can protect cerebral cortical neurons against OGD-induced injury,which may be related to the anti-oxidative stress.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"472"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69847897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type I Brugada syndrome in a Tibetan patient 1例藏族患者的I型Brugada综合征

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00577

Lei Wang, Yang Li, Jianhua Cheng, Qi-gao Zhang

引用次数: 0

Clinical characteristic analysis of 5 patients with confirmed Ebola virus disease 确诊埃博拉病毒病5例临床特征分析

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00584

Fu-xiang Li, Cheng-zhong Li, F. Zhou, Zong-hai Hu, Zhi-hui Chen, C. Hao, Jian-Jun Yang, Chang-xing Guo, Ping Zhang, Ying Cai, Wangang Guo, J. Yin

{"title":"Clinical characteristic analysis of 5 patients with confirmed Ebola virus disease","authors":"Fu-xiang Li, Cheng-zhong Li, F. Zhou, Zong-hai Hu, Zhi-hui Chen, C. Hao, Jian-Jun Yang, Chang-xing Guo, Ping Zhang, Ying Cai, Wangang Guo, J. Yin","doi":"10.3724/SP.J.1008.2015.00584","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00584","url":null,"abstract":"Objective To analyze the clinical characteristics and prognoses of patients with confirmed Ebola virus disease(EVD)and to summarize the clinical treatment experience.Methods The epidemiologic history,symptoms,signs,treatment,and prognoses of 5confirmed EVD cases were summarized.And the relationship between clinical features and clinical outcomes was analyzed.Results The 5patients,2 men and 3women,ranged in age from 32-58 years old,with a median age of 46.Three were severe cases when they were admitted while the others were relatively mild.All the patients admitted the exposure history within 21 days before onset of the symptoms,with two patients(one medical staff)from the same transmission chain.The main symptoms included fever,weakness or fatigue,lack of appetite,diarrhea and red eyes,with one patient having gastrointestinal bleeding.The first test of PCR Ebola virus(EBOV)RNA was positive in all the 5cases.They were promptly isolated and treated with antipyretic and fluid replacement therapy.Meanwhile,prevention of complications and treatment of basic diseases were also conducted.Three patients survived at last and the other two died.Conclusion Prompt isolation of the infection source and tracing the contacts can effectively prevent the transmission of EVD.PCR assay is an effective,rapid and simple method to diagnose EVD.Early supporting treatment-centered multimodality therapy can improve the treatment outcome of EVD.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"584"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estrogen replacement increases β-arrestin2 expression in cardiac tissues in ovariectomized rats 雌激素替代增加去卵巢大鼠心脏组织中β-arrestin2的表达

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00634

Fan Hao, X. Tan, Wei-Zhong Wang, Mingjuan Xu

{"title":"Estrogen replacement increases β-arrestin2 expression in cardiac tissues in ovariectomized rats","authors":"Fan Hao, X. Tan, Wei-Zhong Wang, Mingjuan Xu","doi":"10.3724/SP.J.1008.2015.00634","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00634","url":null,"abstract":"Objective To observe the expression ofβ-arrestin2 in cardiac tissues of ovariectomzied(OVX)rats,and to explore the role ofβ-arrestin2 in estrogen-mediated cardiovascular protection in OVX rats.Methods OVX rats were evaluated by monitoring the serum levels of estrogen and the ratio of uterine weight to body weight.The cardiovascular function of rats was assessed by measuring the ratio of heart weight to body weight,blood pressure and heart rate after estrogen replacement treatment for 4weeks,Western blotting analysis was performed to detect the protein expression ofβ-arrestin1,β-arrestin2 and angiotensin receptor type 1(AT1R)in the cardiac tissues.Results Compared with rats in the sham group,the serum estrogen level and the ratio of uterine weight to body weight in OVX rats were decreased significantly(P0.05);meanwhile,the cardiovascular parameters including blood pressure,heart rate and the ratio of heart weight to body weight were significantly increased(P0.05)in OVX rats,and they were attenuated after treatment with estrogen replacement.Compared with the sham controls,the cardiac expression ofβ-arrestin2 was decreased significantly in OVX rats(P0.05),with up-regulated estrogen expression and unchanged expression ofβ-arrestin1 and AT1R.Conclusion β-Arrestin2 expression is decreased in the cardiac tissues of OVX rats,which can be increased by estrogen replacement therapy.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"634"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69850658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stateflow and queuing theory-based design of emergency medical rescue simulation system 基于状态流和排队理论的紧急医疗救援仿真系统设计

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00649

Jian-fei Pang, Junhua Jiang, Xiao-dong Pan

{"title":"Stateflow and queuing theory-based design of emergency medical rescue simulation system","authors":"Jian-fei Pang, Junhua Jiang, Xiao-dong Pan","doi":"10.3724/SP.J.1008.2015.00649","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00649","url":null,"abstract":"Objective To design an emergency medical rescue simulation system,and to provide reference for optimizing rescue strategy and medical resource allocation by simulating different rescue conditions.Methods Firstly,Stateflow was used to build the main simulation unit,which included arrival of the wounded,classification of the wounded(emergent[type A]and non-emergent[type B]),and medical treatment modules,and then the related event and response function were appended.Secondly,the system parameter input unit and character display unit were set up in Simulink.Finally two different medical treatment strategies(strategy 1:type B wounded waiting for treatment in the rescue module with least waiting patients;strategy 2:type B wounded waiting for treatment in the rescue module with least workload)were simulated and the corresponding rescue strategies were analyzed.Results The system could simulate the medical rescue process of different strategies.The type A wounded could be rescued in a timely manner in both strategies.Type B wounded had a shorter waiting time in strategy 1,but the rescuers had a heavy work load;while they had a longer waiting time in strategy 2,and the rescuers had a lighter work load.Conclusion We have successfully designed a simulation system for emergency medical rescue and its feasibility and effectiveness are verified.Different rescue strategies have their respective advantages and disadvantages in the waiting time and work load,and the two strategies can be combined in practice in order to achieve a dynamic balance between the waiting time of the wounded and the work load of the rescuers.","PeriodicalId":6893,"journal":{"name":"Academic Journal of Second Military Medical University","volume":"36 1","pages":"649"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69850746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ebola virus and innate immunity 埃博拉病毒和先天免疫

Academic Journal of Second Military Medical University Pub Date : 2015-01-01 DOI: 10.3724/SP.J.1008.2015.00607

Jingxi Zhang, Cheng-zhong Li, Chong Bai

引用次数: 0