Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046459
A Danguy, C Decaestecker, F Genten, I Salmon, R Kiss
{"title":"Applications of lectins and neoglycoconjugates in histology and pathology.","authors":"A Danguy, C Decaestecker, F Genten, I Salmon, R Kiss","doi":"10.1159/000046459","DOIUrl":"https://doi.org/10.1159/000046459","url":null,"abstract":"<p><p>The biological importance of oligosaccharide sequences in many different settings is undeniable. Glycan histochemistry has brought together the histological and biochemical approaches and provided insight into the mutual importance of both approaches. The aim of the authors is to take a look at a number of ways in which modern glycohistochemistry contributes to acquiring knowledge about the key role played by carbohydrates in the physiology of vertebrate tissues and human disease. The versatility of lectin and neoglycoconjugate histochemical procedures is emphasized.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"161 1-4","pages":"206-18"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20692630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046438
S A Asala, F E Mbajiorgu, B A Papandro
{"title":"A comparative study of femoral head diameters and sex differentiation in Nigerians.","authors":"S A Asala, F E Mbajiorgu, B A Papandro","doi":"10.1159/000046438","DOIUrl":"https://doi.org/10.1159/000046438","url":null,"abstract":"<p><p>The vertical and transverse diameters of the head of 504 paired human femurs (257 males and 247 females) from Maiduguri in the northeastern arid zone of Nigeria were measured. Identification points and demarking points were determined. The mean diameter of the head of the male femur was significantly greater than that of the female (p <0.001). The identification points that were derived from the vertical and transverse diameters of the head of the femur were higher in males than in females. Those that were derived from the vertical diameters of the head of the male femurs were comparable to the values from the southeastern part of Nigeria. Those that were derived from transverse diameters were lower than the corresponding values from southeastern Nigeria. By means of identification points, more male bones were identified. This was due to the fact that the mean head diameter of female femurs fell within the range of diameters that did not permit positive identification of sex. The male demarking points were also higher than the corresponding female values. Similarly, the demarking point identified the sex of a higher percentage of male femurs. The demarking point, in comparison to the identification point, identified the sex of a smaller percentage of femurs. The results showed that diameters of the femoral head as well as the identification and demarking points that are derived from them are of use in sex and regional identification.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"162 4","pages":"232-7"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046438","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20742714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046471
S Kohl, I Giddings, D Besch, E Apfelstedt-Sylla, E Zrenner, B Wissinger
{"title":"The role of the peripherin/RDS gene in retinal dystrophies.","authors":"S Kohl, I Giddings, D Besch, E Apfelstedt-Sylla, E Zrenner, B Wissinger","doi":"10.1159/000046471","DOIUrl":"https://doi.org/10.1159/000046471","url":null,"abstract":"<p><p>Peripherin/RDS is a transmembrane glycoprotein expressed in vertebrate photoreceptors. It is located at the rim of the disc membranes of the photoreceptor outer segments, where it is thought to play an important role in folding and stacking of the discs. Initially, the identification of a mutation in the rds mouse model defined the role of this gene in hereditary retinal dystrophies. To date over 60 different mutations have been reported in human retinal diseases, with most being restricted to single families. A characteristic of mutations in the peripherin/RDS gene is the broad phenotypic spectrum in patients, and the variability in clinical expression, even within families. Thus, genotype-phenotype correlations are difficult and only reliable for a minority of mutations.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"162 2-3","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20742863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046496
R Gatti, S Ferretti, G Bucci, M Simonazzi, G Orlandini
{"title":"Histomorphology of an ileal orthotopic neobladder: 24 Year's follow-up.","authors":"R Gatti, S Ferretti, G Bucci, M Simonazzi, G Orlandini","doi":"10.1159/000046496","DOIUrl":"https://doi.org/10.1159/000046496","url":null,"abstract":"<p><p>No morphological data are so far available about long-term (over 15 years) adaptation and outcome of ileal neobladders. In this single case report we have studied a 72-year-old man who underwent reconstruction of an ileal neobladder 24 years ago. The ileal epithelium shows changes toward a colonic aspect with villous atrophy and increased goblet cell number although, as in normal ileum, sialomucins are the most abundant secretory products. Neuroendocrine cells are reduced to a few elements of a single morphological class. Even in the presence of a low apoptotic rate reduced cell proliferation causes mucosal thinning. These adaptive mechanisms grant an excellent clinical and functional result even beyond 20 years after surgery. It is suggested that ileal neobladder can constitute an important model for the study of epithelial differentiation control.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"163 4","pages":"179-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046496","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20945313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046501
P Hanson, S P Magnusson, H Sorensen, E B Simonsen
{"title":"Differences in the iliolumbar ligament and the transverse process of the L5 vertebra in young white and black people.","authors":"P Hanson, S P Magnusson, H Sorensen, E B Simonsen","doi":"10.1159/000046501","DOIUrl":"https://doi.org/10.1159/000046501","url":null,"abstract":"<p><p>The anatomy of the iliolumbar ligament (ILL) and the spatial orientation of the transverse process of the L5 vertebra were studied in 62 young black (n = 29) and white (n = 33) men and women during routine autopsy. The aim of the study was to determine possible racial differences in the structure and attachments of the iliolumbar ligament. The present study also investigated the spatial orientation of the transverse process of the L5 vertebra since the ILL has been reported to attach to the transverse process. The measurements of the iliolumbar ligament were carried out with a digital vernier caliper while the transverse process angles were measured with an adjustable protractor. The ligament in black people was made up of a single, markedly longer band compared to white people, where the ligament was made up of two shorter bands. The ILL measured 61.8 +/- 1.3 mm in black and 33.2 +/- 1.5 mm white men, and in black women 61.3 +/- 0.9 mm versus 32.2 +/- 1.2 mm in white women (p <0.01). Further, the ILL was markedly wider in black than white subjects (p <0.01). The horizontal and vertical angle also varied greatly between black and white subjects (p <0.01). These are previously unrecognized observations. Albeit unsubstantiated, these findings may have implications for understanding the etiology of various low back stress problems.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"163 4","pages":"218-23"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20946361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046451
S Hakomori
{"title":"Cancer-associated glycosphingolipid antigens: their structure, organization, and function.","authors":"S Hakomori","doi":"10.1159/000046451","DOIUrl":"https://doi.org/10.1159/000046451","url":null,"abstract":"<p><p>Experimental and human cancers are often characterized by the presence of tumor-associated glycosphingolipid (GSL) antigens defined by monoclonal antibodies. Major progress has been made during the past two decades on structural identification of these antigens. None of these structures are truly 'tumor-specific'. However, many of the antibodies show preferential or 'specific' reactivity with tumors, based on organizational differences of membrane GSLs in tumor cells versus normal cells. Clustered GSL antigens organized with transducer molecules in microdomain have been found recently to comprise a structural and functional unit involved in tumor cell adhesion coupled with signal transduction. Some of the GSL antigens have been identified as adhesion molecules recognized by carbohydrate-binding proteins or by complementary carbohydrates on target cells. Such adhesion, coupled with signaling, may initiate the metastatic process. Elucidating the mechanism of this initial adhesion/signaling step may lead to discovery of therapeutic agents that disrupt adhesion ('antiadhesion therapy') or normalize signaling ('ortho-signaling therapy'). Tumor-associated GSL antigens are also a target in immunotherapy of tumors, including development of antitumor vaccines.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"161 1-4","pages":"79-90"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046451","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20693441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046449
H Geyer, R Geyer
{"title":"Strategies for glycoconjugate analysis.","authors":"H Geyer, R Geyer","doi":"10.1159/000046449","DOIUrl":"https://doi.org/10.1159/000046449","url":null,"abstract":"Glycoconjugates such as glycoproteins and glycosphingolipids exhibit multiple functions in biological systems. A correlation of functional features with defined structural parameters, however, presupposes detailed information on the glycoconjugates’ carbohydrate moieties including the type of linkage of the oligosaccharide chain(s), monosaccharide composition as well as sequence, linkage positions and anomeric configurations of individual sugar monomers. Chemical and biochemical analyses are often impeded by the limited amounts of sample available and the vast structural heterogeneity of glycoconjugate glycans, thus requiring highly sensitive and efficient methods for detection, separation and structural elucidation of these carbohydrates. The aim of the article is to present suitable strategies for structural characterization of glycoconjugate glycans and to briefly review some of the techniques commonly used in this field.","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"161 1-4","pages":"18-35"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20693512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046454
H Rüdiger
{"title":"Plant lectins - more than just tools for glycoscientists: occurrence, structure, and possible functions of plant lectins.","authors":"H Rüdiger","doi":"10.1159/000046454","DOIUrl":"https://doi.org/10.1159/000046454","url":null,"abstract":"<p><p>Plant lectins are easily available, fairly stable and suitable for many kinds of chemical modification. Thus, they have become important tools in glycosciences. In the present review, it is attempted to throw light upon aspects of lectinology that deal with their natural occurrence, biosynthesis, structure, binding specificities and hypotheses about their biological functions.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"161 1-4","pages":"130-52"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046454","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20694046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046437
P Hanson, S P Magnusson, E B Simonsen
{"title":"Differences in sacral angulation and lumbosacral curvature in black and white young men and women.","authors":"P Hanson, S P Magnusson, E B Simonsen","doi":"10.1159/000046437","DOIUrl":"https://doi.org/10.1159/000046437","url":null,"abstract":"The angle between the base of the sacrum and its pelvic surface (SA), and the lumbosacral curve (LSC) defined as the anteroposterior distance between the L1 vertebra and the sacrum were studied in 105 fresh cadavers aged 15–32 years at death. The sample is comprised of 27 black males, 20 black females, 37 white males and 21 white females. Measurements of the SA were made with an adjustable protractor with an accuracy of ±1°. A new method using a specially designed instrument was developed for taking LSC measurements. A sliding scale that could be moved in the cranialcaudal direction was fitted with a measurement stick which measured the depth of the lumbar and sacral vertebral bodies (ventral to dorsal). The measuring device was placed on the autopsy table above the supine cadaver to measure these depths from the ventral side. The LSC was calculated as the mean difference between lumbar and sacral depths. The SA is smaller in black than in white men (43.6 ± 3.4 vs. 61.7 ± 2.1°, p <0.001). Similarly, black women have a smaller SA than white women (36.0 ± 2.2 vs. 50.2 ± 1.9°, p <0.001). The LSC is also larger in black than white men (9.4 ± 1.1 vs. 5.1 ± 0.3 cm, p <0.001), and black women also have significantly larger LSC compared to white women (13.2 ± 0.5 vs. 9.2 ± 0.6 cm, p <0.001). These data are so far the largest empirical evidence of differences in the SA and the LSC in young black and white cadaver specimens.","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"162 4","pages":"226-31"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046437","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20742713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1998-01-01DOI: 10.1159/000046442
U Sommer, M Kressin, B Schnorr
{"title":"Electron-microscopic detection of cytoskeletal actin in the epithelial cells of bovine abomasum.","authors":"U Sommer, M Kressin, B Schnorr","doi":"10.1159/000046442","DOIUrl":"https://doi.org/10.1159/000046442","url":null,"abstract":"<p><p>The fine structural distribution of cytoskeletal actin was investigated in the oxyntic gland region and in the pyloric gland region of bovine abomasal mucosa using immunoelectron methods. In all exocrine cell types of the gastric epithelium, actin filaments exhibit a polarized pattern of distribution. In the members of the mucus- and protein-synthesizing cell line, the actin filaments form a prominent barrier to the exocytosis of secretory granules underneath the apical membrane. Therefore, actin is supposed to be involved in the control of exocytosis. The basolateral subplasmalemmal cortex of the exocrine cells, however, shows weaker labeling for actin filaments. In this position actin might be responsible for the movement of epithelial cells along the length of the gastric gland during physiological cell renewal and for the maintenance of the integrity of the epithelial unit and of cell polarization. In parietal cells of bovine abomasum, actin filaments are, in addition, associated with the intracellular canaliculus, but not with the tubulovesicular compartment. We assume that actin filaments reorganize the canalicular membrane after membrane translocations in the course of acid secretion. For the first time, actin filaments were documented in the rare brush cell type of bovine abomasum, where they do not form a terminal web beneath the apical membrane and therefore point out the nonexocrine function of the brush cell type. In all polarized cells of the bovine abomasum, actin filaments underlay the two apical members of the junctional complex, the tight junction and the adherens junction, where they might be involved in the regulation of cell adhesion, cell motility and cell shape determination.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"163 1","pages":"20-30"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000046442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20761634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}