Strategies for glycoconjugate analysis.

Acta anatomica Pub Date : 1998-01-01 DOI:10.1159/000046449
H Geyer, R Geyer
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引用次数: 49

Abstract

Glycoconjugates such as glycoproteins and glycosphingolipids exhibit multiple functions in biological systems. A correlation of functional features with defined structural parameters, however, presupposes detailed information on the glycoconjugates’ carbohydrate moieties including the type of linkage of the oligosaccharide chain(s), monosaccharide composition as well as sequence, linkage positions and anomeric configurations of individual sugar monomers. Chemical and biochemical analyses are often impeded by the limited amounts of sample available and the vast structural heterogeneity of glycoconjugate glycans, thus requiring highly sensitive and efficient methods for detection, separation and structural elucidation of these carbohydrates. The aim of the article is to present suitable strategies for structural characterization of glycoconjugate glycans and to briefly review some of the techniques commonly used in this field.
糖缀合物分析策略。
糖蛋白和鞘糖脂等糖缀合物在生物系统中具有多种功能。然而,功能特征与定义的结构参数的相关性,预设了糖缀合物的碳水化合物部分的详细信息,包括低聚糖链的连接类型、单糖组成以及单个糖单体的序列、连接位置和端粒构型。化学和生化分析常常受到可用样品数量有限和糖缀合聚糖巨大的结构异质性的阻碍,因此需要高度敏感和高效的方法来检测、分离和阐明这些碳水化合物的结构。本文的目的是提出合适的策略糖缀合聚糖的结构表征,并简要回顾了一些常用的技术在这一领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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