美国分子生物学期刊(英文)最新文献

{"title":"c-Myc Knockout as a Model for Gene Editing for Training Healthcare Professional Students","authors":"Prema S. Rao, U. Subrahmanyeswara Rao","doi":"10.4236/ajmb.2023.134018","DOIUrl":"https://doi.org/10.4236/ajmb.2023.134018","url":null,"abstract":"Correction of genetic errors, commonly known as gene editing, holds promise to treat diseases with unmet medical needs. However, gene therapy trials do encounter unwanted outcomes, because of an incomplete understanding of the disease states, and gene therapy processes, among others. This situation encourages a concept that healthcare professionals receiving laboratory research training will not only identify inadequacies in basic biomedical knowledge of gene therapies but also provide tangible refinements. To this end, we have undertaken the PharmD student training in gene editing in a basic research laboratory setting. As a model, MYC gene was chosen for knockout using CRISPR-Cas9 method in HT29 and OVCAR8 cells. Students were involved in the design of MYC-specific gRNAs, subcloning into Cas9-carrying plasmid, and selection of knockout clones from the transfected cells. Subsequently, genomic DNA isolation and sequencing, analysis of clonal DNA sequences using online bioinformatics tools, western blotting, cell proliferation and cell division cycle experiments, were performed to characterize the MYC knockout clones. Results presented in this communication suggest that healthcare professionals who received laboratory training gain a better understanding of the disease states and mechanisms, gene therapy protocols, limitations of gene therapies, ability to critically evaluate the literature and confidence in the oversight of gene therapies in the clinic.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135053485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Performance of Microscopy and Nested PCR for the Detection of <i>Cryptosporidium</i> Species in Patients Living with HIV/Aids in Abidjan (Côte d’Ivoire)","authors":"N’docho Assi Fiacre-Tanguy, Gonédélé Bi Sery Ernest, Tuo Karim, Bialé Marina Danielle D., N’Guessan Tiacoh Landry, Koffi Attoungbré Clément, Koffi David, A. Bérenger, V. Henriette, Touré O. André","doi":"10.4236/ajmb.2023.131002","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131002","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into Genetic Diversity of Cultivated Lima Bean (<i>Phaseolus lunatus</i> L.) in Benin","authors":"Rose Fernande Fagbédji, Lambert Gustave Djedatin, C. Nanoukon, Géofroy Kinhoégbè, Amed Havivi, H. Yédomonhan, C. Agbangla","doi":"10.4236/ajmb.2023.131003","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131003","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso","authors":"Théodora Mahoukèdè Zohoncon, Shoukrat Ohuwa Toyin Bello, Prosper Bado, Rogomenoma Alice Ouédraogo, Estelle Ouédraogo, Ina Marie Angèle Traoré, Abdoul Karim Ouattara, Florencia Wenkunni Djigma, Albert Théophane Yonli, Assita Sanou-Lamien, Olga Mélanie Lompo, Jacques Simpore","doi":"10.4236/ajmb.2023.134019","DOIUrl":"https://doi.org/10.4236/ajmb.2023.134019","url":null,"abstract":"Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. The cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: This study aims to detect the presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. Methods: This descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with TaqMan probes. Results: The mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%; 95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV DNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135106697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Host Interferon-<i>γ</i> Gene Polymorphism with <i>Toxoplasma gondii</i> Infection in Pregnant Women of Bangladesh","authors":"N. Akter, S. Tamanna, Molie Rahman, Atiqur Rahman, A. M. Hasan, T. Rahman","doi":"10.4236/ajmb.2023.133011","DOIUrl":"https://doi.org/10.4236/ajmb.2023.133011","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of Hepatitis B Virus Immune Escape Mutants in Kenya","authors":"Rhoda Elizabeth King, J. Kimotho, R. Macharia, Faith Ndung'u, S. Nzou, R. M. Irekwa","doi":"10.4236/ajmb.2023.131001","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131001","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Profiling of Sugarcane Wine, a Traditional Fermented Beverage (Loungouila) from Madingou","authors":"Yannick Frédéric Okouakoua, N. Mokémiabeka, C. A. Kayath, Moïse Doria Kaya-Ongoto, S. A. Nzaou, Nkalla Lambi Ndelani, Etienne Nguimbi","doi":"10.4236/ajmb.2023.134014","DOIUrl":"https://doi.org/10.4236/ajmb.2023.134014","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ampelographic and Genetic Diversity Assessment of Some Local Grape Genotypes under Egyptian Conditions","authors":"R. Mahmoud, A. A. Dahab, Gehan Ahmed Mahmoud, M. El-Wahab, E. El-Bassel, E. Mahdy","doi":"10.4236/ajmb.2023.133013","DOIUrl":"https://doi.org/10.4236/ajmb.2023.133013","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Polyclonal Antibody for the Diagnosis of Human African Trypanosomiasis","authors":"Dawala Koromtili Oumar, Matthew Mutinda Munyao, A. Mwangi, R. Waihenya, Peter Kipkemboi Rotich, R. M. Irekwa, Tonny Teya Nyandwaro, C. W. Njoroge, J. Yego, Primrose Muthoni Ndungu, Sharlene Kerubo Mageto, Damaris Mutethya Kilei, Otilmoi Poul Stephen, Nicole Sian Tanchu, Grace Ng’endo Kanyita, Shingo Inoue, Lucy Gitau, S. Nzou","doi":"10.4236/ajmb.2023.132009","DOIUrl":"https://doi.org/10.4236/ajmb.2023.132009","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70514064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcal Pyrogenic Exotoxin Genes SpeA and SpeB in Isolates of <i>Streptococcus pyogenes</i> from Children with Pharyngitis, Gezira State, Sudan","authors":"Minas Mohamed Balla, A. Mergani, Mohamed Elamin A. M. E. Medani, A. Abakar, A. Dafalla","doi":"10.4236/ajmb.2022.124015","DOIUrl":"https://doi.org/10.4236/ajmb.2022.124015","url":null,"abstract":"Background: Streptococcus pyogenes (group A streptococcus, GAS) is an important human bacterial pathogen. This organism possesses many virulence factors, Streptococcal pyrogenic exotoxinone of these. Aim: Detection of Streptococcal pyrogenic exotoxin SpeA and SpeB in isolated Streptococcus pyogenes. Methods: Tow hundred throat swab samples were collected from children with pharyngitis referred to Pediatric Teaching hospital and ENT hospital Wad medani, Sudan, from January to November 2021. The ques-tionnaire was filled out to collect clinical and demographic data. Throat swabs were collected and processed with the standard microbiological pro-cedure to isolate Streptococcus pyogenes. Antimicrobial susceptibility testing was done on all GAS isolates using the Kirby Bauer disk diffusion method according to clinical laboratory standard institute (CLSI) guidelines. Detection of Spy 1258 gene and Streptococcal pyrogenic exotoxins SpeA and SpeB were done by using Multiplex PCR. Results: Amongst the Tow hundred collected samples fifty-one isolates (25.5%) were identified as S. pyogenes. Antibiotic susceptibility testing showed that all the GAS isolates were sensitive to Azithromycin and Penicillin. Sensitivity to Erythromycin, Gentamicin, Clarithromycin, Amoxicillin and Cephalexin were 88.2%, 86.3%, 45.1%, 41.2%, 13.7%, respectively. SpeA was detected in 17 (33.3%) and SpeB in 48 (94.1%). Conclusion: Streptococcal pyrogenic exotoxin genes SpeA and SpeB were detected in 17 (33.3%) and 48 (94.1%) respectively of Streptococcus pyogenes isolates.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}