美国分子生物学期刊(英文)最新文献_第3页

The Protective Effects of Preventive Atomisation Inhalation of Edaravone on the Lung Tissues of Rats with Smoke Inhalation Injury 预防性雾化吸入依达拉奉对烟雾吸入性损伤大鼠肺组织的保护作用

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124013

Ruluo Qian, Changshuan Xiao, Yaping Liu, Jingzhe Yang

引用次数: 0

The Roles of pH in the Modification of Wild-Type Recombinant Phlebia radiata Manganese Peroxidase 3 Activities and Stability of Secondary Structures pH对重组放线菌锰过氧化物酶3活性及二级结构稳定性的影响

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124014

U. Ufot, I. Okop, Mfoniso P. Uko, Imekan Isaac Akpan, Khasim Cali, M. Akpanabiatu

引用次数: 0

Effect of cAMP and cGMP on Connexin43 Expression in Isolated Human and Bovine Ciliary Epithelium cAMP和cGMP对人、牛睫状上皮连接蛋白43表达的影响

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.123009

Ning Ma, Xiaohong Li, Renyi Wu

引用次数: 0

Diversity and Distribution of Staphylococcal Chromosomal Cassettes Mec (SCCmec) Types I, II and III in Coagulase-Negative Staphylococcal Strains Isolated from Surfaces and Medico-Technical Materials of the University Hospital of Abomey-Calavi/Sô-Ava Abomey-Calavi大学医院表面和医疗技术材料中分离的凝固酶阴性葡萄球菌I型、II型和III型葡萄球菌染色体卡带Mec (SCCmec)的多样性和分布/Sô-Ava

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.123011

Nanoukon Chimène, C. Fresnel, Djèdatin Gustave, Sina Haziz, Socohou Akim, Dado Aurel, Kougblènou Enorck, Badé Farid, Agbangla Clémént, Baba-Moussa Lamine Saïd

{"title":"Diversity and Distribution of Staphylococcal Chromosomal Cassettes Mec (SCCmec) Types I, II and III in Coagulase-Negative Staphylococcal Strains Isolated from Surfaces and Medico-Technical Materials of the University Hospital of Abomey-Calavi/Sô-Ava","authors":"Nanoukon Chimène, C. Fresnel, Djèdatin Gustave, Sina Haziz, Socohou Akim, Dado Aurel, Kougblènou Enorck, Badé Farid, Agbangla Clémént, Baba-Moussa Lamine Saïd","doi":"10.4236/ajmb.2022.123011","DOIUrl":"https://doi.org/10.4236/ajmb.2022.123011","url":null,"abstract":"The coagulase-negative staphylococci (CoNS) have long been considered to be low pathogenicity. The possibility of a horizontal transfer of resistance and virulence genes from S. aureus to CoNS could increase the pathogenicity of these bacteria. showed resistance to methicillin. However, only 28.5% (20/70) carried the mecA gene. SCCmec was identified in only 17.14% (12/70) of these strains. Four strains carried mecA gene as well as one of the three types of SCCmec searched. SCCmec types I, II and III were identified in CoNS strains studied. SCCmec type I was the most frequent chromosomal cassette in mecA + strains, only or in association with another SCCmec. The study also revealed methicillin-resistant strains carrying SCCmec lacking the mecA gene. Finally, 60% (12/20) of the strains were found to be non-typeable. Our results show that CoNS strains present a high resistance to methicillin and the source of this resistance in the CoNS of our study is not only the mecA gene. There is also a high diversity of SCCmec, justified by a large number of non-typeable CoNS strains. The mecA − SCCmec + methicillin-resistant strains deserve to be sequenced for further studies.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on PVL, blaOXA-23 and blaOXA-51 Genes in Drug Resistant Staphylococcus aureus Causing Surgical-Sites and Traumatic Wounds Infections, Sudan 苏丹耐药金黄色葡萄球菌引起手术部位和创伤创面感染的PVL、blaOXA-23和blaOXA-51基因研究

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.122004

S. Yousif, A. Abakar, S. Elzaki, Salma Ibrahim, O. A. Elhasan, Mohamed Taj-Eldin, E. A. Ahmed

引用次数: 0

pH-Dependence of Manganese (II) Oxidation Reaction by Novel Wild-Type and Mutants Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3) Enzymes 新型野生型和突变型重组放线菌锰过氧化物酶3 (pr - mnp3)氧化反应的ph依赖性

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.122007

U. Ufot, M. Akpanabiatu, Khasim Cali, I. Uffia, I. Udosen

{"title":"pH-Dependence of Manganese (II) Oxidation Reaction by Novel Wild-Type and Mutants Recombinant <i>Phlebia radiata</i> Manganese Peroxidase 3 (rPr-MnP3) Enzymes","authors":"U. Ufot, M. Akpanabiatu, Khasim Cali, I. Uffia, I. Udosen","doi":"10.4236/ajmb.2022.122007","DOIUrl":"https://doi.org/10.4236/ajmb.2022.122007","url":null,"abstract":"The goal of this study was to determine whether mutation of the Mn-binding site of wild-type recombinant Phlebia radiata manganese peroxidase 3 affected the pH-dependence kinetic parameters. pH range investigated was 2.5 – 12.0. The catalytic efficiency of the mutant enzymes at high and low pH in comparison to the wild-type was investigated using standard rPr-MnP3 protocol. Wild-type recombinant Phlebia radiata MnP3 enzyme showed optimal activity with Mn (II) as substrate at pH 5.0 and remained moderately active (approximately 40%) in the pH range of 6.0 - 9.0. The rPr-MnP3 mutants’ maximum activity ranged between 5.5 and 8.0. Wild-type and mutants rPr-MnP3 enzymes exhibited a similar pH profile with optimum pH of 3.0 for ABTS oxidation. Mutation has severely decreased the catalytic efficiency for Mn (II) oxidation at pH 5.0. The rPr-MnP3 enzymes showed enhanced affinity for Mn (II) at alkaline pH and a more alkaline range for catalysis than ever reported for any Manganese Peroxidase. This study reveals that at higher pH, rPr-MnP3 can function with alternative ligands in the Mn (II) site and does not have an absolutely obligate requirement for an all carboxylate ligand set. These results further strongly confirm that Mn 2+ binding site is the only productive catalytic site for Mn (II) oxidation.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Retrospective Analysis of Wastewater Confirms Dominant Circulation of SARS-CoV-2 Delta Variant in Nairobi, Kenya, between April 2021 and August 2021 一项废水回顾性分析证实，2021年4月至2021年8月期间，肯尼亚内罗毕的SARS-CoV-2三角洲变体占主导地位

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.123010

Patson Sichamba, F. Wamunyokoli, P. Borus, R. Nzunza, Opanda Silvanos, S. Symekher, S. Khamadi, J. Majanja, Meshack Wadegu, Esther Chitechi, Benlick Mwangi, Evans Komen, Stephen Ombija, W. Bulimo

引用次数: 0

Role of Tyrosine Kinase Receptors in Growth Factor Mediated Signal Transduction, with Specific Reference to MAPK/Rasand p13k-Akt Containing Pathways in Oncogenesis: A Qualitative Database Review 酪氨酸激酶受体在生长因子介导的信号转导中的作用，具体涉及肿瘤发生中包含MAPK/Rasand p13k-Akt的途径:定性数据库综述

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124012

Chanjugaa Uthayakumar, Rajavarthani Sanjeev

{"title":"Role of Tyrosine Kinase Receptors in Growth Factor Mediated Signal Transduction, with Specific Reference to MAPK/Rasand p13k-Akt Containing Pathways in Oncogenesis: A Qualitative Database Review","authors":"Chanjugaa Uthayakumar, Rajavarthani Sanjeev","doi":"10.4236/ajmb.2022.124012","DOIUrl":"https://doi.org/10.4236/ajmb.2022.124012","url":null,"abstract":"Receptor Tyrosine kinases (RTKs) play a crucial role in the signal transduction pathways at cellular levels. RTK plays a vital role in cellular communica-tion and transmission of signals to the adjacent cells and regulates different functions of the cell, such as cellular growth, differentiation, metabolism and motility. RTK s triggers growth factor receptors such as epidermal growth factor, insulin growth factor-1 receptor, platelet derived growth factor receptor, and fibro blast growth factor receptor and vascular endothelial growth factor receptor, thereby initiating and regulating cell growth and proliferation. MAPK/RAS and PI3/AKT pathways are the major pathways of RTK’s function. Dysregulation of these RTK’s and pathways often leads to many diseases such as Noonan Syndrome, Logius Syndrome, CFC syndrome and different types of cancer. Point mutation and over expression of receptors and mutations in Ras leads to 30% of human cancers. Also over expression of different growth factor receptors by RTK too lead to several types of cancers as Glioblastoma, Thyroid cancer, Colon cancer and Non-small cell lung cancer. PTEN mutation in PI3/AKT pathway often leads to carcinoma relative to Thyroid, Skin, Large intestine, eye and Bone. Therefore, these RTK’s often used as targets for cancer therapies. The medical sector uses various","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in Ipomoea batatas L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions 甘薯真核翻译起始因子4E (eIF4E)基因家族的克隆与鉴定，以了解六倍体甘薯与病毒的相互作用

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124017

A. Brown, M. Egnin, Foaziatu Bukari, O. Idehen, I. Ritte, D. Mortley, Gregory C. Bernard, D. Alexander, C. Bonsi

引用次数: 0

Molecular Cloning, Expression and Purification of Recombinant VHH Proteins Expressed in E. coli 大肠杆菌表达重组VHH蛋白的分子克隆、表达及纯化

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114011

Tewodros Fentahun Jember

{"title":"Molecular Cloning, Expression and Purification of Recombinant VHH Proteins Expressed in E. coli","authors":"Tewodros Fentahun Jember","doi":"10.4236/ajmb.2021.114011","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114011","url":null,"abstract":"Variable Heavy-chain Homodimer (VHH) or Nanobody is a recombinant dromedary antibody fragment which is classified as the smallest antibody fragments with the highest binding affinity and specificity of the original whole antibody. In this study the Expression of Nanobodies in E. coli WK6 cell periplasm was performed. The protein expression and purity was and analyzed by Affinity Chromatography, SDS PAGE and Western Blot. Upon elution with Imidazole, the concentrations observed using the OD280 nm of the eluted fractions EI, E2 and E3 were observed to be 0.42 μg/ml, 0.13 μg/ml and −0.46 μg/ml respectively. This gives an Antilog of 7.88 kDa which showed the calculated molecular size of our band. The SDS-PAGE gel reading was confirmed using Western blot analysis and illustrated as the specific binding of the mouse Anti-His antibody to the Histidine tag of the Nanobody. The Nanobody protein expression was then analyzed further with western blotting showed a strong signal at the region corresponding to the 15 kDa marker indicating presence of the Nanobody gene. This was taken as further confirmation of the protein expression from the bacterial cells.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45116555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1