美国分子生物学期刊(英文)最新文献

{"title":"Effect of High Glucose and Carboxymethyl-Lysine on Osteocyte Gene Expression.","authors":"Rachana Vaidya, Lauren Conlon, Olivia Duclos, Ramina Behzad, Jacob Aaronson, Lamya Karim","doi":"10.4236/ajmb.2025.152012","DOIUrl":"10.4236/ajmb.2025.152012","url":null,"abstract":"<p><p>Diabetes mellitus (DM) is associated with increased bone fragility despite normal or elevated bone mineral density, partially due to the accumulation of advanced glycation end products (AGEs) in bone tissue. AGEs, such as carboxymethyl lysine (CML), impair osteocyte function by activating the receptor for advanced glycation end products (RAGE), triggering oxidative stress and inflammatory responses. This study aimed to investigate the effects of high glucose (HG) and CML on bone remodeling, glycation, inflammatory markers, and cellular functions in osteocytes. Using the murine osteocyte cell line OCY454-12H, we treated cells with HG (30 mM glucose) or 3 μM CML to simulate diabetic conditions. We assessed the expression of bone remodeling markers (SOST, RANKL, OPG, CTsK), glycation markers (RAGE, AGER1), inflammatory cytokines (IL-6, TNF-<i>α</i>), and cellular functions, including proliferation, viability, and apoptosis, using quantitative PCR and functional assays. HG treatment resulted in a 10-fold increase in SOST expression (9.3 vs. 0.9, p ≤ 0.0001) and a 2.4-fold increase in RANKL expression (2.75 vs. 1.15, p ≤ 0.0001), with a concurrent 2-fold increase in OPG (2.60 vs. 1.04, p ≤ 0.0001). The RANKL/OPG ratio remained unchanged (p = 0.15). HG also significantly increased RAGE expression by 3.67-fold (4.20 vs. 1.15, p ≤ 0.0001) and AGER1 by 1.65-fold (1.94 vs. 1.15, p ≤ 0.0001), along with a 2.02-fold increase in IL-6 (2.32 vs. 1.12, p ≤ 0.001) and a 7.35-fold increase in TNF-<i>α</i> (7.04 vs. 1.04, p ≤ 0.0001). Cell viability and proliferation were significantly higher under HG, accompanied by increased caspase-3 activity, indicating enhanced apoptosis. In contrast, CML exposure significantly upregulated RAGE (3.18 vs. 1.15, p ≤ 0.0001) and AGER1 (2.10 vs. 1.14, p = 0.028) but had no significant effects on bone remodeling markers, inflammatory cytokines, or cellular functions at physiological concentrations. Our findings demonstrate that HG disrupts osteocyte function by altering bone remodeling, glycation, and inflammatory pathways, while CML at physiological levels selectively activates glycation markers without inducing broader cellular dysfunction. These results underscore the role of the AGE-RAGE axis in diabetic bone fragility and highlight the need for future <i>in vivo</i> studies to explore therapeutic strategies targeting AGE accumulation and RAGE signaling in bone.</p>","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"15 2","pages":"150-169"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144121583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA Methyltransferase Inhibitors Induce Cerebral Dopamine Neurotrophic Factor Expression in C6 Glioma Cells","authors":"Sumeya Z. Mukhtar, L. Niles","doi":"10.4236/ajmb.2023.133012","DOIUrl":"https://doi.org/10.4236/ajmb.2023.133012","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Haplotypes in Exon 4 of KLK2 Gene with Raised Serum Prostate-Specific Antigen","authors":"I. Ogbu, Ogochukwu A. Nwankwo, Chinemerem C. Ogbu, E. Nna, Kingsley K. Anya, Malachy O. Odoh, D. C. Obasi","doi":"10.4236/ajmb.2023.131005","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131005","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Two Molecular Diagnostic Tests for COVID-19: Abbott RealTime SARS-CoV-2 and Allplex&#8482; 2019-nCoV, in the Epidemic Context in Senegal","authors":"B. Faye, Oulimata Gueye, D. Niang, B. Mbengue, A. Dièye","doi":"10.4236/ajmb.2023.131004","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131004","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics Identification of ZNFs/LINC00520/miR-181d/BCL2 Axis as a Novel Network in Cisplatin-Resistant Lung Adenocarcinoma Cells","authors":"Ying Xu, Na Guo, Jinghan Guo, Dongze Wang, Qian Xu, Xianglin Li, Zhengxin Zhang, Hongbin Yang, Ruxing Wang, Xiurong Zhao, Lei Liu","doi":"10.4236/ajmb.2023.131006","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131006","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cannabidiol-Mediated Sequestration of Alzheimer’s Amyloid-<i>β</i> Peptides in ADDL Oligomers","authors":"Yang Li, Fengyuan Zhang, C. Herron, Ivonne Rosales, A. Heredia, Nicolae-Viorel Buchete, B. Rodriguez","doi":"10.4236/ajmb.2023.132008","DOIUrl":"https://doi.org/10.4236/ajmb.2023.132008","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70514022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Helicobacter pylori</i> Virulence Genes <i>cagA</i>, <i>babA2</i>, and <i>vacA</i> Detection in Dyspeptic Patients from Burkina Faso","authors":"T. Compaore, Kalifou Traoré, N. I. Compaoré, L. Traoré, Sylvie Zida, S. Soubeiga, Dinanibé Kambire, J. P. Ouédraogo, Aminata Sidibé, Yasmine Astrid Sana, T. Sagna, W. F. Djigma, H. Ouédraogo, J. Simporé","doi":"10.4236/ajmb.2023.133010","DOIUrl":"https://doi.org/10.4236/ajmb.2023.133010","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70514083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and Evaluation of Wb-SXP-1 and Wb-123 Recombinant Antigens as Potential Diagnostic Biomarkers for Lymphatic Filariasis","authors":"Sharlene Kerubo Mageto, R. Waihenya, A. Mwangi, Peter Kipkemboi Rotich, Matthew Mutinda Munyao, Tonny Teya, R. M. Irekwa, J. Yego, C. W. Njoroge, Grace Ng’endo Kanyita, Nicole Sian Tanchu, Dawala Koromtili Oumar, Primrose Muthoni Ndungu, S. Nzou","doi":"10.4236/ajmb.2023.132007","DOIUrl":"https://doi.org/10.4236/ajmb.2023.132007","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70514006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Performance of Microscopy and Nested PCR for the Detection of <i>Cryptosporidium</i> Species in Patients Living with HIV/Aids in Abidjan (Côte d’Ivoire)","authors":"N’docho Assi Fiacre-Tanguy, Gonédélé Bi Sery Ernest, Tuo Karim, Bialé Marina Danielle D., N’Guessan Tiacoh Landry, Koffi Attoungbré Clément, Koffi David, A. Bérenger, V. Henriette, Touré O. André","doi":"10.4236/ajmb.2023.131002","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131002","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into Genetic Diversity of Cultivated Lima Bean (<i>Phaseolus lunatus</i> L.) in Benin","authors":"Rose Fernande Fagbédji, Lambert Gustave Djedatin, C. Nanoukon, Géofroy Kinhoégbè, Amed Havivi, H. Yédomonhan, C. Agbangla","doi":"10.4236/ajmb.2023.131003","DOIUrl":"https://doi.org/10.4236/ajmb.2023.131003","url":null,"abstract":"","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}