美国分子生物学期刊(英文)最新文献_第4页

pH-Dependence of Manganese (II) Oxidation Reaction by Novel Wild-Type and Mutants Recombinant Phlebia radiata Manganese Peroxidase 3 (rPr-MnP3) Enzymes 新型野生型和突变型重组放线菌锰过氧化物酶3 (pr - mnp3)氧化反应的ph依赖性

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.122007

U. Ufot, M. Akpanabiatu, Khasim Cali, I. Uffia, I. Udosen

{"title":"pH-Dependence of Manganese (II) Oxidation Reaction by Novel Wild-Type and Mutants Recombinant <i>Phlebia radiata</i> Manganese Peroxidase 3 (rPr-MnP3) Enzymes","authors":"U. Ufot, M. Akpanabiatu, Khasim Cali, I. Uffia, I. Udosen","doi":"10.4236/ajmb.2022.122007","DOIUrl":"https://doi.org/10.4236/ajmb.2022.122007","url":null,"abstract":"The goal of this study was to determine whether mutation of the Mn-binding site of wild-type recombinant Phlebia radiata manganese peroxidase 3 affected the pH-dependence kinetic parameters. pH range investigated was 2.5 – 12.0. The catalytic efficiency of the mutant enzymes at high and low pH in comparison to the wild-type was investigated using standard rPr-MnP3 protocol. Wild-type recombinant Phlebia radiata MnP3 enzyme showed optimal activity with Mn (II) as substrate at pH 5.0 and remained moderately active (approximately 40%) in the pH range of 6.0 - 9.0. The rPr-MnP3 mutants’ maximum activity ranged between 5.5 and 8.0. Wild-type and mutants rPr-MnP3 enzymes exhibited a similar pH profile with optimum pH of 3.0 for ABTS oxidation. Mutation has severely decreased the catalytic efficiency for Mn (II) oxidation at pH 5.0. The rPr-MnP3 enzymes showed enhanced affinity for Mn (II) at alkaline pH and a more alkaline range for catalysis than ever reported for any Manganese Peroxidase. This study reveals that at higher pH, rPr-MnP3 can function with alternative ligands in the Mn (II) site and does not have an absolutely obligate requirement for an all carboxylate ligand set. These results further strongly confirm that Mn 2+ binding site is the only productive catalytic site for Mn (II) oxidation.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Retrospective Analysis of Wastewater Confirms Dominant Circulation of SARS-CoV-2 Delta Variant in Nairobi, Kenya, between April 2021 and August 2021 一项废水回顾性分析证实，2021年4月至2021年8月期间，肯尼亚内罗毕的SARS-CoV-2三角洲变体占主导地位

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.123010

Patson Sichamba, F. Wamunyokoli, P. Borus, R. Nzunza, Opanda Silvanos, S. Symekher, S. Khamadi, J. Majanja, Meshack Wadegu, Esther Chitechi, Benlick Mwangi, Evans Komen, Stephen Ombija, W. Bulimo

引用次数: 0

Role of Tyrosine Kinase Receptors in Growth Factor Mediated Signal Transduction, with Specific Reference to MAPK/Rasand p13k-Akt Containing Pathways in Oncogenesis: A Qualitative Database Review 酪氨酸激酶受体在生长因子介导的信号转导中的作用，具体涉及肿瘤发生中包含MAPK/Rasand p13k-Akt的途径:定性数据库综述

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124012

Chanjugaa Uthayakumar, Rajavarthani Sanjeev

{"title":"Role of Tyrosine Kinase Receptors in Growth Factor Mediated Signal Transduction, with Specific Reference to MAPK/Rasand p13k-Akt Containing Pathways in Oncogenesis: A Qualitative Database Review","authors":"Chanjugaa Uthayakumar, Rajavarthani Sanjeev","doi":"10.4236/ajmb.2022.124012","DOIUrl":"https://doi.org/10.4236/ajmb.2022.124012","url":null,"abstract":"Receptor Tyrosine kinases (RTKs) play a crucial role in the signal transduction pathways at cellular levels. RTK plays a vital role in cellular communica-tion and transmission of signals to the adjacent cells and regulates different functions of the cell, such as cellular growth, differentiation, metabolism and motility. RTK s triggers growth factor receptors such as epidermal growth factor, insulin growth factor-1 receptor, platelet derived growth factor receptor, and fibro blast growth factor receptor and vascular endothelial growth factor receptor, thereby initiating and regulating cell growth and proliferation. MAPK/RAS and PI3/AKT pathways are the major pathways of RTK’s function. Dysregulation of these RTK’s and pathways often leads to many diseases such as Noonan Syndrome, Logius Syndrome, CFC syndrome and different types of cancer. Point mutation and over expression of receptors and mutations in Ras leads to 30% of human cancers. Also over expression of different growth factor receptors by RTK too lead to several types of cancers as Glioblastoma, Thyroid cancer, Colon cancer and Non-small cell lung cancer. PTEN mutation in PI3/AKT pathway often leads to carcinoma relative to Thyroid, Skin, Large intestine, eye and Bone. Therefore, these RTK’s often used as targets for cancer therapies. The medical sector uses various","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Characterization of Eukaryotic Translation Initiation Factor 4E (eIF4E) Gene Family in Ipomoea batatas L. (Lam) for Understanding Hexaploid Sweetpotato-Virus Interactions 甘薯真核翻译起始因子4E (eIF4E)基因家族的克隆与鉴定，以了解六倍体甘薯与病毒的相互作用

美国分子生物学期刊(英文) Pub Date : 2022-01-01 DOI: 10.4236/ajmb.2022.124017

A. Brown, M. Egnin, Foaziatu Bukari, O. Idehen, I. Ritte, D. Mortley, Gregory C. Bernard, D. Alexander, C. Bonsi

引用次数: 0

Molecular Cloning, Expression and Purification of Recombinant VHH Proteins Expressed in E. coli 大肠杆菌表达重组VHH蛋白的分子克隆、表达及纯化

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114011

Tewodros Fentahun Jember

{"title":"Molecular Cloning, Expression and Purification of Recombinant VHH Proteins Expressed in E. coli","authors":"Tewodros Fentahun Jember","doi":"10.4236/ajmb.2021.114011","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114011","url":null,"abstract":"Variable Heavy-chain Homodimer (VHH) or Nanobody is a recombinant dromedary antibody fragment which is classified as the smallest antibody fragments with the highest binding affinity and specificity of the original whole antibody. In this study the Expression of Nanobodies in E. coli WK6 cell periplasm was performed. The protein expression and purity was and analyzed by Affinity Chromatography, SDS PAGE and Western Blot. Upon elution with Imidazole, the concentrations observed using the OD280 nm of the eluted fractions EI, E2 and E3 were observed to be 0.42 μg/ml, 0.13 μg/ml and −0.46 μg/ml respectively. This gives an Antilog of 7.88 kDa which showed the calculated molecular size of our band. The SDS-PAGE gel reading was confirmed using Western blot analysis and illustrated as the specific binding of the mouse Anti-His antibody to the Histidine tag of the Nanobody. The Nanobody protein expression was then analyzed further with western blotting showed a strong signal at the region corresponding to the 15 kDa marker indicating presence of the Nanobody gene. This was taken as further confirmation of the protein expression from the bacterial cells.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45116555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphological, Pathological, Biochemical and Molecular Characterization of Ralstonia solanacearum Isolates in Bangladesh 孟加拉茄枯菌分离株的形态、病理、生化和分子特征

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114012

S. Razia, M. Chowdhury, F. Aminuzzaman, N. Sultana, M. Islam

{"title":"Morphological, Pathological, Biochemical and Molecular Characterization of Ralstonia solanacearum Isolates in Bangladesh","authors":"S. Razia, M. Chowdhury, F. Aminuzzaman, N. Sultana, M. Islam","doi":"10.4236/ajmb.2021.114012","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114012","url":null,"abstract":"One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of Ralstonia solanacearum were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and R. solanacearum was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. R. solanacearum is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of R. solanacerum.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43110825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Prediction of Monophyletic Groups Based on Gene Order and Sequence Similarity in Organelle DNA 基于组织器DNA基因顺序和序列相似性的单类群预测

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114008

M. Cserhati

引用次数: 0

Occurrence, Molecular Characterization and Phylogenetic Relationship of Aspergillus Species Isolated from Garri Sold in Benue State, North Central, Nigeria 尼日利亚中北部贝努埃州Garri产曲霉的发生、分子特征及系统发育关系

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114009

I. O. Ogbonna, I. Amai, C. Aguoru, Dooshima Charity Amai

{"title":"Occurrence, Molecular Characterization and Phylogenetic Relationship of Aspergillus Species Isolated from Garri Sold in Benue State, North Central, Nigeria","authors":"I. O. Ogbonna, I. Amai, C. Aguoru, Dooshima Charity Amai","doi":"10.4236/ajmb.2021.114009","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114009","url":null,"abstract":"Characterization and identification of molds based on cultural and morphological characteristics are often not reliable and frail with limitations. The occurrence of Aspergillus species in garri on sale in markets in Benue State, Nigeria, was studied by molecular techniques. Aspergillus species were isolated and purified on Potato Dextrose Agar. DNA from the purified isolates was extracted using the ZR fungal DNA miniprep and amplified by PCR mix made up of 12.5 μL of Taq 2X Master Mix. Primer sequences for the fungi characterization were internal transcribed spacers ITS 4 and ITS 5. The phylogenetic tree was plotted between the isolated organisms and reference sequences and evolutionary analysis was conducted in MEGA X. Result revealed that one thousand, six hundred and forty-six Aspergilli were isolated comprising of 980 and 666 isolates from the white and yellow garri respectively. Aspergillus flavus, A. fumigatus, A. niger, A. aculeatinus, and A. aculeatus were identified. Twenty percent (20%) of the strains had aflatoxin D structural gene, 50% amplified AFLP and 70% of the strains expressed AFLQ genes needed for the biosynthesis of aflatoxin B1. Majority of the strains that showed the expression of these structural genes were consistent with Aspergillus flavus. Phylogenetic tree showed a close relationship among the isolates and their most identical sequence in the NCBI database.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43806095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Possible Link of Intestinal Microbiota and Type 2 Diabetes Mellitus 肠道菌群与2型糖尿病可能联系的评估

美国分子生物学期刊(英文) Pub Date : 2021-06-18 DOI: 10.4236/ajmb.2021.113006

G. Simeon, Arikekpar Ibemologi, Dienize George Cameron

引用次数: 0

Joint Effects of Mexidol and Nitroglycerine on Nitric Oxide Formation in Animal Liver Tissues 墨西多和硝化甘油对动物肝组织一氧化氮形成的联合作用

美国分子生物学期刊(英文) Pub Date : 2021-06-18 DOI: 10.4236/ajmb.2021.113007

T. Zhumabaeva, Z. Kuropteva, Zh. T. Moldaliev, Nazgul Zhumabaeva, A. Kadyrbaeva, Nurbek Bopoev, Z. Abdullaeva

引用次数: 0