美国分子生物学期刊(英文)最新文献_第4页

Morphological, Pathological, Biochemical and Molecular Characterization of Ralstonia solanacearum Isolates in Bangladesh 孟加拉茄枯菌分离株的形态、病理、生化和分子特征

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114012

S. Razia, M. Chowdhury, F. Aminuzzaman, N. Sultana, M. Islam

{"title":"Morphological, Pathological, Biochemical and Molecular Characterization of Ralstonia solanacearum Isolates in Bangladesh","authors":"S. Razia, M. Chowdhury, F. Aminuzzaman, N. Sultana, M. Islam","doi":"10.4236/ajmb.2021.114012","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114012","url":null,"abstract":"One thirty samples (fifty-five potato tubers, twenty-seven potato stems, three chili stems, twenty-eight soil samples, five weed samples, three banana leaves, and nine water samples) were examined and one hundred six (106) Bangladeshi isolates of Ralstonia solanacearum were isolated and identified. Isolation was made on selective media (Tetrazolium chloride media) and R. solanacearum was identified based on morphological, pathological and biochemical properties and polymerase chain reaction (PCR) by using the species-specific primers. Studies showed that 81.54% (106) samples were positive on tetrazolium chloride solid medium. Among them 90 isolates were virulent and rest of them were avirulent. Fifty isolates were selected for chemical characterization based on hypersensitivity test. R. solanacearum is gram negative, aerobic facultative bacteria on the basis of chemical characterization. Fifty tested isolates expressed as race 3 while in biovar test forty-eight showed as biovar III and the rest two showed as biovar I. In nine tested isolates from the three districts a species-specific band of 280 bp was amplified in PCR that confirmed the identity of R. solanacerum.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43110825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Prediction of Monophyletic Groups Based on Gene Order and Sequence Similarity in Organelle DNA 基于组织器DNA基因顺序和序列相似性的单类群预测

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114008

M. Cserhati

引用次数: 0

Occurrence, Molecular Characterization and Phylogenetic Relationship of Aspergillus Species Isolated from Garri Sold in Benue State, North Central, Nigeria 尼日利亚中北部贝努埃州Garri产曲霉的发生、分子特征及系统发育关系

美国分子生物学期刊(英文) Pub Date : 2021-08-20 DOI: 10.4236/ajmb.2021.114009

I. O. Ogbonna, I. Amai, C. Aguoru, Dooshima Charity Amai

{"title":"Occurrence, Molecular Characterization and Phylogenetic Relationship of Aspergillus Species Isolated from Garri Sold in Benue State, North Central, Nigeria","authors":"I. O. Ogbonna, I. Amai, C. Aguoru, Dooshima Charity Amai","doi":"10.4236/ajmb.2021.114009","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114009","url":null,"abstract":"Characterization and identification of molds based on cultural and morphological characteristics are often not reliable and frail with limitations. The occurrence of Aspergillus species in garri on sale in markets in Benue State, Nigeria, was studied by molecular techniques. Aspergillus species were isolated and purified on Potato Dextrose Agar. DNA from the purified isolates was extracted using the ZR fungal DNA miniprep and amplified by PCR mix made up of 12.5 μL of Taq 2X Master Mix. Primer sequences for the fungi characterization were internal transcribed spacers ITS 4 and ITS 5. The phylogenetic tree was plotted between the isolated organisms and reference sequences and evolutionary analysis was conducted in MEGA X. Result revealed that one thousand, six hundred and forty-six Aspergilli were isolated comprising of 980 and 666 isolates from the white and yellow garri respectively. Aspergillus flavus, A. fumigatus, A. niger, A. aculeatinus, and A. aculeatus were identified. Twenty percent (20%) of the strains had aflatoxin D structural gene, 50% amplified AFLP and 70% of the strains expressed AFLQ genes needed for the biosynthesis of aflatoxin B1. Majority of the strains that showed the expression of these structural genes were consistent with Aspergillus flavus. Phylogenetic tree showed a close relationship among the isolates and their most identical sequence in the NCBI database.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43806095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Possible Link of Intestinal Microbiota and Type 2 Diabetes Mellitus 肠道菌群与2型糖尿病可能联系的评估

美国分子生物学期刊(英文) Pub Date : 2021-06-18 DOI: 10.4236/ajmb.2021.113006

G. Simeon, Arikekpar Ibemologi, Dienize George Cameron

引用次数: 0

Joint Effects of Mexidol and Nitroglycerine on Nitric Oxide Formation in Animal Liver Tissues 墨西多和硝化甘油对动物肝组织一氧化氮形成的联合作用

美国分子生物学期刊(英文) Pub Date : 2021-06-18 DOI: 10.4236/ajmb.2021.113007

T. Zhumabaeva, Z. Kuropteva, Zh. T. Moldaliev, Nazgul Zhumabaeva, A. Kadyrbaeva, Nurbek Bopoev, Z. Abdullaeva

引用次数: 0

Characteristics of a Critical Calcium Transportation Regulator: Plasma Membrane Ca2+-ATPase Involved in Calcium Homeostasis from Hyriopsis cumingii (Lea) 一种关键钙转运调节因子：三角帆蚌（Lea）质膜Ca2+-ATP酶参与钙稳态的特性

美国分子生物学期刊(英文) Pub Date : 2021-03-26 DOI: 10.4236/AJMB.2021.112004

A. Zhang, Zhiming Zhou

{"title":"Characteristics of a Critical Calcium Transportation Regulator: Plasma Membrane Ca2+-ATPase Involved in Calcium Homeostasis from Hyriopsis cumingii (Lea)","authors":"A. Zhang, Zhiming Zhou","doi":"10.4236/AJMB.2021.112004","DOIUrl":"https://doi.org/10.4236/AJMB.2021.112004","url":null,"abstract":"Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca2+ out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca2+-ATPase gene was isolated from the gill of Hyriopsis cumingii (HcPMCA) by using SMART RACE technique. The entire cDNA was 5230 bp, including a 417-bp 5'-UTR, a 3588-bp ORF and a 1225-bp 3'-UTR, encoding a 1195-amino acid protein, and no putative signal peptide was predicted. Compared with PMCA homologs from seawater mollusks, HcPMCA had high similarity with them in both sequence and structure. Tissue-specific expression analysis revealed that HcPMCA mRNA was detected in all the sampled tissues, but was prominently expressed in the gill and mantle. When exposed to a serie of increasing Ca2+ that lasted for 7 days, the mRNA expression of HcPMCA in the mantle was slightly downregulated, but peaked at 60 mg/L. Moreover, the temporal expression of HcPMCA transcripts in the mantle after 60 mg/L Ca2+ exposure was shown to be bell-shaped, which was slightly downregulated at 24 h, but upregulated from 24 h to 48 h post-treatment, peaking at 48 h. The result of present study provides useful information for further studies on function and regulation mechanism of HcPMCA gene.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"11 1","pages":"38-50"},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46197151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-γ 难表达蛋白、人IL-12和IFN-γ的表达

美国分子生物学期刊(英文) Pub Date : 2021-03-04 DOI: 10.4236/AJMB.2021.112003

Yoshihito Hosaka, S. Matsutani, Shinya Kawate, K. Itoh, A. Miura, Yukaze Mizoura, Sayumi Yamada, S. Uemura, H. Konno, Ewa Grave, H. Wakui, H. Itoh

引用次数: 2

A Study of Prevalence and Pathogenic Activity of Bacteria in the Air of Dhaka City and Their Antimicrobial Resistance Pattern 达卡市空气中细菌的患病率、致病性及其耐药性模式研究

美国分子生物学期刊(英文) Pub Date : 2021-03-04 DOI: 10.4236/AJMB.2021.112005

S. Nawar, M. Rashid, A. Ahmed, Mahboob Hossain

引用次数: 1

The Anti-Sickling Properties of Medicinal Plants, Insights in Botanical Medicine* 药用植物抗镰刀病特性，植物医学见解*

美国分子生物学期刊(英文) Pub Date : 2021-01-01 DOI: 10.4236/ajmb.2021.114013

Ioannis Krasias

{"title":"The Anti-Sickling Properties of Medicinal Plants, Insights in Botanical Medicine*","authors":"Ioannis Krasias","doi":"10.4236/ajmb.2021.114013","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114013","url":null,"abstract":"SCD is one of the most prevailing homogeneous inherited haemoglobinopathies causing a plethora of various clinical complications to the patients. The high mortality and morbidity severely concern the Western community, where numerous clinical trials and research for a cure are in process. In order to al-leviate patients from the severe symptoms of the disease, avoiding the side effects, Botanical Medicine exhibits concrete evidence, as a gold candidate, to be the salvation to the problem. The Preferred Reporting Items for Systematic Review (PRISMA) protocol has been used to achieve extensive research on the topic, focusing on the identification and evaluation of the phytochemical properties of common medicinal plants. Meta-analysis has also been imple-mented on the results of published literature. Forest plots have been plotted, comparing and evaluating the results’ validity and significance. The meta-analysis results have undoubtedly demonstrated the importance and significance of the medicinal plants and their properties against various clinical complications, focusing on the pathogenicity of SCD. Surprisingly, their effectiveness to suppress haemoglobin polymerisation and increase the Fe 2+ /Fe 3+ ratio in patients, en-hanced the normal morphological erythrocytes’ appearance by suppressing the sickle shape of drepanocytes. Research made on the epidemiology of SCD asso-ciates the disease with the geographical frequency of malaria infection. Based on the natural selection theory of Charles Darwin, nature aids in the popula-tion’s survival by the endemicity of various medicinal plants in areas with increased SCD patients. Limitations to the medicinal plants’ consumptions and further therapeutic options have been discussed.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Degradation of Mitochondria by Mitophagy in Pathogenic Fungi 病原真菌线粒体自噬的选择性降解

美国分子生物学期刊(英文) Pub Date : 2021-01-01 DOI: 10.4236/ajmb.2021.111002

Jane Sadhna Jagernath, Shuai Meng, Jiehua Qiu, Shi Huanbin, Yanjun Kou

{"title":"Selective Degradation of Mitochondria by Mitophagy in Pathogenic Fungi","authors":"Jane Sadhna Jagernath, Shuai Meng, Jiehua Qiu, Shi Huanbin, Yanjun Kou","doi":"10.4236/ajmb.2021.111002","DOIUrl":"https://doi.org/10.4236/ajmb.2021.111002","url":null,"abstract":"Selective mitochondrial autophagy or mitophagy is an evolutionarily conserved cellular process that selectively degrades superfluous, damaged, and dysfunctional mitochondria. This process is believed to be a mitochondrial quality control system crucial for intracellular homeostasis. Recently, researchers developed a range of methods to induce mitophagy and a variety of assays to monitor this process. With these new methods, the research on mitophagy has been developed rapidly. In particular, some key receptors and regulatory factors in fungi have been identified, which provides a basis for further understanding of the mechanism of this process. Although it has been studied extensively in the model yeast Saccharomyces cerevisiae, mitophagy in pathogenic fungi remains poorly understood. However recent studies have shown that mitophagy is involved in the regulation of pathogenicity of pathogenic fungi, which greatly increases the importance of mitophagy. Therefore, it is necessary to review the current research on mitophagy in order to provide an accurate understanding of mitophagy and promote mitophagy research in the pathogenic fungi.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":"11 1","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1