美国分子生物学期刊(英文)最新文献_第5页

Characteristics of a Critical Calcium Transportation Regulator: Plasma Membrane Ca2+-ATPase Involved in Calcium Homeostasis from Hyriopsis cumingii (Lea) 一种关键钙转运调节因子：三角帆蚌（Lea）质膜Ca2+-ATP酶参与钙稳态的特性

美国分子生物学期刊(英文) Pub Date : 2021-03-26 DOI: 10.4236/AJMB.2021.112004

A. Zhang, Zhiming Zhou

{"title":"Characteristics of a Critical Calcium Transportation Regulator: Plasma Membrane Ca2+-ATPase Involved in Calcium Homeostasis from Hyriopsis cumingii (Lea)","authors":"A. Zhang, Zhiming Zhou","doi":"10.4236/AJMB.2021.112004","DOIUrl":"https://doi.org/10.4236/AJMB.2021.112004","url":null,"abstract":"Plasma Membrane Calcium ATPase (PMCA) plays a critical role in transporting Ca2+ out of the cytosol across the plasma membrane. Here, a full-length cDNA sequence of plasma membrane Ca2+-ATPase gene was isolated from the gill of Hyriopsis cumingii (HcPMCA) by using SMART RACE technique. The entire cDNA was 5230 bp, including a 417-bp 5'-UTR, a 3588-bp ORF and a 1225-bp 3'-UTR, encoding a 1195-amino acid protein, and no putative signal peptide was predicted. Compared with PMCA homologs from seawater mollusks, HcPMCA had high similarity with them in both sequence and structure. Tissue-specific expression analysis revealed that HcPMCA mRNA was detected in all the sampled tissues, but was prominently expressed in the gill and mantle. When exposed to a serie of increasing Ca2+ that lasted for 7 days, the mRNA expression of HcPMCA in the mantle was slightly downregulated, but peaked at 60 mg/L. Moreover, the temporal expression of HcPMCA transcripts in the mantle after 60 mg/L Ca2+ exposure was shown to be bell-shaped, which was slightly downregulated at 24 h, but upregulated from 24 h to 48 h post-treatment, peaking at 48 h. The result of present study provides useful information for further studies on function and regulation mechanism of HcPMCA gene.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46197151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Difficult-to-Express Proteins, Human IL-12 and IFN-γ 难表达蛋白、人IL-12和IFN-γ的表达

美国分子生物学期刊(英文) Pub Date : 2021-03-04 DOI: 10.4236/AJMB.2021.112003

Yoshihito Hosaka, S. Matsutani, Shinya Kawate, K. Itoh, A. Miura, Yukaze Mizoura, Sayumi Yamada, S. Uemura, H. Konno, Ewa Grave, H. Wakui, H. Itoh

引用次数: 2

A Study of Prevalence and Pathogenic Activity of Bacteria in the Air of Dhaka City and Their Antimicrobial Resistance Pattern 达卡市空气中细菌的患病率、致病性及其耐药性模式研究

美国分子生物学期刊(英文) Pub Date : 2021-03-04 DOI: 10.4236/AJMB.2021.112005

S. Nawar, M. Rashid, A. Ahmed, Mahboob Hossain

引用次数: 1

The Anti-Sickling Properties of Medicinal Plants, Insights in Botanical Medicine* 药用植物抗镰刀病特性，植物医学见解*

美国分子生物学期刊(英文) Pub Date : 2021-01-01 DOI: 10.4236/ajmb.2021.114013

Ioannis Krasias

{"title":"The Anti-Sickling Properties of Medicinal Plants, Insights in Botanical Medicine*","authors":"Ioannis Krasias","doi":"10.4236/ajmb.2021.114013","DOIUrl":"https://doi.org/10.4236/ajmb.2021.114013","url":null,"abstract":"SCD is one of the most prevailing homogeneous inherited haemoglobinopathies causing a plethora of various clinical complications to the patients. The high mortality and morbidity severely concern the Western community, where numerous clinical trials and research for a cure are in process. In order to al-leviate patients from the severe symptoms of the disease, avoiding the side effects, Botanical Medicine exhibits concrete evidence, as a gold candidate, to be the salvation to the problem. The Preferred Reporting Items for Systematic Review (PRISMA) protocol has been used to achieve extensive research on the topic, focusing on the identification and evaluation of the phytochemical properties of common medicinal plants. Meta-analysis has also been imple-mented on the results of published literature. Forest plots have been plotted, comparing and evaluating the results’ validity and significance. The meta-analysis results have undoubtedly demonstrated the importance and significance of the medicinal plants and their properties against various clinical complications, focusing on the pathogenicity of SCD. Surprisingly, their effectiveness to suppress haemoglobin polymerisation and increase the Fe 2+ /Fe 3+ ratio in patients, en-hanced the normal morphological erythrocytes’ appearance by suppressing the sickle shape of drepanocytes. Research made on the epidemiology of SCD asso-ciates the disease with the geographical frequency of malaria infection. Based on the natural selection theory of Charles Darwin, nature aids in the popula-tion’s survival by the endemicity of various medicinal plants in areas with increased SCD patients. Limitations to the medicinal plants’ consumptions and further therapeutic options have been discussed.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Degradation of Mitochondria by Mitophagy in Pathogenic Fungi 病原真菌线粒体自噬的选择性降解

美国分子生物学期刊(英文) Pub Date : 2021-01-01 DOI: 10.4236/ajmb.2021.111002

Jane Sadhna Jagernath, Shuai Meng, Jiehua Qiu, Shi Huanbin, Yanjun Kou

{"title":"Selective Degradation of Mitochondria by Mitophagy in Pathogenic Fungi","authors":"Jane Sadhna Jagernath, Shuai Meng, Jiehua Qiu, Shi Huanbin, Yanjun Kou","doi":"10.4236/ajmb.2021.111002","DOIUrl":"https://doi.org/10.4236/ajmb.2021.111002","url":null,"abstract":"Selective mitochondrial autophagy or mitophagy is an evolutionarily conserved cellular process that selectively degrades superfluous, damaged, and dysfunctional mitochondria. This process is believed to be a mitochondrial quality control system crucial for intracellular homeostasis. Recently, researchers developed a range of methods to induce mitophagy and a variety of assays to monitor this process. With these new methods, the research on mitophagy has been developed rapidly. In particular, some key receptors and regulatory factors in fungi have been identified, which provides a basis for further understanding of the mechanism of this process. Although it has been studied extensively in the model yeast Saccharomyces cerevisiae, mitophagy in pathogenic fungi remains poorly understood. However recent studies have shown that mitophagy is involved in the regulation of pathogenicity of pathogenic fungi, which greatly increases the importance of mitophagy. Therefore, it is necessary to review the current research on mitophagy in order to provide an accurate understanding of mitophagy and promote mitophagy research in the pathogenic fungi.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70513540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nanobody cDNA Mock-Up in pHEN6c Plasmid Vector: Live Out 纳米体cDNA在pHEN6c质粒载体上的模拟:活出

美国分子生物学期刊(英文) Pub Date : 2021-01-01 DOI: 10.4236/ajmb.2021.114010

Tewodros Fentahun Jember

引用次数: 0

Self-Assembly of Amyloid-Beta and Its Piezoelectric Properties 淀粉样蛋白的自组装及其压电性能

美国分子生物学期刊(英文) Pub Date : 2020-12-18 DOI: 10.4236/ajmb.2021.111001

I. Rosales, Laura Salazar, Daniel Luna, A. Negrón, I. Bdikin, B. Rodriguez, A. Heredia

引用次数: 0

The Prevalence of Human Metapneumovirus among Children with Acute Respiratory Tract Infection in North Kordofan State, Sudan 苏丹北科尔多凡州急性呼吸道感染儿童中人偏肺病毒的流行情况

美国分子生物学期刊(英文) Pub Date : 2020-09-09 DOI: 10.4236/ajmb.2020.104017

H. Ahmed, Nassreldeen Khalid Abdelrahman, M. A. Adam

引用次数: 0

Identification of JAK2 (V617F) Mutation in Myeloproliferative Neoplasms by Using Allele Specific Polymerase Chain Reaction (AS-PCR) 利用等位基因特异性聚合酶链反应(AS-PCR)鉴定骨髓增殖性肿瘤中JAK2 (V617F)突变

美国分子生物学期刊(英文) Pub Date : 2020-09-09 DOI: 10.4236/ajmb.2020.104019

K. Tun, A. Z. Latt, Win Naing, S. Htwe, Y. K. Ko, W. Mar, S. Hlaing, Wai Wai Han, S. Win

{"title":"Identification of JAK2 (V617F) Mutation in Myeloproliferative Neoplasms by Using Allele Specific Polymerase Chain Reaction (AS-PCR)","authors":"K. Tun, A. Z. Latt, Win Naing, S. Htwe, Y. K. Ko, W. Mar, S. Hlaing, Wai Wai Han, S. Win","doi":"10.4236/ajmb.2020.104019","DOIUrl":"https://doi.org/10.4236/ajmb.2020.104019","url":null,"abstract":"Myeloproliferative neoplasms (MPNs) are a group of clonal haematopoietic stem cell disorders characterized by the proliferation of one or more myeloid cell lineages. According to WHO classification, the Janus associated kinase 2 (JAK2) V617F mutation is one of the major diagnostic criteria in BCR-ABL1 negative myeloproliferative neoplasms. The aim of this study is to detect the JAK2 (V617F) mutation in patients with myeloproliferative neoplasms to get accurate diagnosis and proper management. A total of 90 clinically diagnosed MPN patients attending to Department of Clinical Haematology, Yangon General Hospital were enrolled in this study. The mean age was 53.4 ± 14 years which ranged from 16 to 81 years old and male and female ratio was 2.4:1. The identification of JAK2 (V617F) point mutation was found to be positive in 44/90 MPN patients (48.9%). According to MPN subtypes, the JAK2 mutation positivity was found in 19 out of 46 polycythemia vera patients (41.3%), 17 out of 25 essential thrombocythemia patients (68%), 8 out of 15 primary myelofibrosis patients (53.3%), 0 of 4 others myeloproliferative neoplasms (0%). Confirmation of each of nine JAK2 mutation positive and negative samples was done by Sanger sequencing. The arterial or venous thrombotic attack was found in 32/44 JAK2 mutation positive cases (72.7%) and 12/44 JAK2 mutation negative cases (27.3%). The association between thrombotic attack and presence of JAK2 mutation was statistically significance with p = 0.000. The diagnosis of myeloproliferative neoplasms mainly relies on the molecular genetics according to WHO classification. The Allele specific PCR reaction is sensitive, simple test and relatively cost-effective. Therefore, the identification of JAK2 (V617F) somatic point mutation by AS-PCR should be implemented as a routine diagnosis procedure for patients with chronic and suspected myeloproliferative neoplasms.","PeriodicalId":65391,"journal":{"name":"美国分子生物学期刊(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43417587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of DNA Extraction Methods for Detection of Leishmania by Polymerase Chain Reaction 聚合酶链反应检测利什曼原虫DNA提取方法的评价

美国分子生物学期刊(英文) Pub Date : 2020-09-09 DOI: 10.4236/ajmb.2020.104018

Leticia Surian Batalini, S. Castro, Carla Geórgia Rodrigues Guimarães Souto, H. Neitzke-Abreu, M. Lima-Júnior

引用次数: 0