2008 2nd International Conference on Bioinformatics and Biomedical Engineering最新文献_第8页

BMP-2 Modified Hydroxyapatite/Chitosan Composite Scaffold for Bone Engineering BMP-2改性羟基磷灰石/壳聚糖复合骨工程支架

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.218

Ai-jun Zuo, Dong-chun Liang, Pei Sun, Rui Zhang, R. Zhao, Baoli Wang, G. Guo, Jingyu-Zhang

{"title":"BMP-2 Modified Hydroxyapatite/Chitosan Composite Scaffold for Bone Engineering","authors":"Ai-jun Zuo, Dong-chun Liang, Pei Sun, Rui Zhang, R. Zhao, Baoli Wang, G. Guo, Jingyu-Zhang","doi":"10.1109/ICBBE.2008.218","DOIUrl":"https://doi.org/10.1109/ICBBE.2008.218","url":null,"abstract":"To improve the osteoinductive capability of hydroxyapatite/chitosan composite(HCC), which was previously demonstrated of perfect biocompatibility, osteoconductive ability and biodegradability, recombinant human bone morphogenetic protein 2(rhBMP-2) modification was applied to HCC. The osteogenetic ability of this new complex were detected both in vitro and in vivo. Mouse bone marrow stroma cells(mMSCs) were primarily cultured and seeded on either boneBMP-2 modified HCC(BHCC) or unmodified HCC. All the cell seeded scaffolds were cultured in osteoinductive medium, alkaline phosphatase (ALP) activity and osteocalcin (ocn) gene expression were evaluated at different time, cell morphology was investigated by scanning electron microscope (SEM) at 5 days culture and in vito calcification was detected at 14 days culture. To further detect its in vivo osteogenetic capability, mMSCs seeded scaffolds were implanted into athymic mice backs subcutaneously, ectopic bone formation was verified by X-ray examination and histomorphological staining. As a result, compared with HCC, cells on BHCC showed markedly increased ALP activity and ocn gene expression thought out the experiments, more proper and functional cell morphology could been seen by SEM, larger area of calcium deposition was revealed by Alizarin red S staining. At eight weeks implantation apparent new bone formation was supported by X-ray images and H&E stained slides. The findings of this study indicate the potential of BHCC as an ideal bone tissue engineering scaffold.","PeriodicalId":6399,"journal":{"name":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","volume":"11 1","pages":"888-892"},"PeriodicalIF":0.0,"publicationDate":"2008-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82949344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization and the Immune Regulation Activities in Vitro of Se-GL-P, an Antioxidant Selenium-Containing Protein from Se-Enriched Ganoderma Lucidum Mushroom 富硒灵芝抗氧化含硒蛋白Se-GL-P的表征及体外免疫调节活性

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.44

Ming Du, Lanwei Zhang, Xiaosong Hu, Guanghua Zhao

{"title":"Characterization and the Immune Regulation Activities in Vitro of Se-GL-P, an Antioxidant Selenium-Containing Protein from Se-Enriched Ganoderma Lucidum Mushroom","authors":"Ming Du, Lanwei Zhang, Xiaosong Hu, Guanghua Zhao","doi":"10.1109/ICBBE.2008.44","DOIUrl":"https://doi.org/10.1109/ICBBE.2008.44","url":null,"abstract":"Our research group has purified an antioxidant selenium-containing protein (Se-GL-P) from Se-enriched Ganoderma Lucidum (Se-GL), its characteristics has been determined partly. In this study, the characteristics of Se-GL-P were identified further, in which the contents of selenocysteine (Se-Cys) and selenomethionine (Se-Met), isoelectric point (pi), and peptide mass fingerprint (PMF) were involved. Moreover, in vitro, the effects on multiplication of T and B lymphocyte cell and killing activity of NK cell were determined by method of MTS. The effect on secretion of TNF-alpha was determined by method of ELISA. Results showed that Se-GL-P was a kind of new selenium-containing protein proved by method of peptide mass fingerprint. 31.275 % of Selenium in total selenium amino acids was in form of Se-Cys, and 68.725% of that was in form of Se- Met. All groups with different doses of Se-GL-P could promote the multiplication of T and B lymphocyte cell of mouse as well as promoting the killing activity of NK cell. However, Se-GL-P had no better effect on TNF-alpha secretion by macrophages in mouse. At the same concentration, the immune regulation activity of Se-GL- P was higher than crude protein from Se-GL (SeGLPr). The immune regulation activity of the former was almost 1.5 times of the latter. Consequently, the results indicated that the Se-GL-P could be studied further, as a kind of bioactivity component with high immune regulation activity.","PeriodicalId":6399,"journal":{"name":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","volume":"1 1","pages":"156-162"},"PeriodicalIF":0.0,"publicationDate":"2008-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86733694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signal Component Analysis by Use of a Diffusion Model and its Application for Autonomic Nerve Function Evaluation 基于扩散模型的信号分量分析及其在自主神经功能评价中的应用

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.689

Yifa Jiang, H. Ye, Qing Zhou, Shishao Liu

引用次数: 0

Simulation and Assessment of Surface Water Quality in Response to Urban Land Use Change 城市土地利用变化对地表水水质的模拟与评价

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.1108

J. Zhou, Y. Liu, J. Chen, S. Zeng, F. Yu

引用次数: 0

Nonlinear Contrast Enhancement of Medical Ultrasonic Image Based on Munsell's Scale 基于孟塞尔尺度的医学超声图像非线性对比度增强

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.988

Xiang Nanfei, Wang Tian-fu, Zou Yuanwen, L. Deyu, Lin Jiangli

引用次数: 0

Stability Analysis of the Pelvis after Type I Resection of Iliac Tumor 髂I型肿瘤切除术后骨盆稳定性分析

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.788

Jia Yongwei, Chen Liming, Yu Guangrong, Yu Yan, Lou Yongjian, Yang Zhiyong, N. Ming, Chen Bo, Ding Zuquan

{"title":"Stability Analysis of the Pelvis after Type I Resection of Iliac Tumor","authors":"Jia Yongwei, Chen Liming, Yu Guangrong, Yu Yan, Lou Yongjian, Yang Zhiyong, N. Ming, Chen Bo, Ding Zuquan","doi":"10.1109/ICBBE.2008.788","DOIUrl":"https://doi.org/10.1109/ICBBE.2008.788","url":null,"abstract":"The purpose of this study is to analyze the pelvic stability after type I resection of iliac tumor. Six adult cadaveric specimens were tested. The iliac subtotal resection models were established according to Ennecking' s type I resection. Markers were affixed to the key regions of the pelves. Axial loading from proximal lumbar was applied by MTS load cell in the gradient from ON to 500 N in the double feet standing state. Images in front view were obtained using CCD (charge coupled device). Based on Image J software, displacements of SI (the first sacral vertebrae) of the resected pelves and the intact pelves were calculated respectively using digital marker tracing method with center-of-mass algorithm. Serious instabilities were found in the resected pelves. The rotational movements SI around the normal side femoral head of the resected pelvis were found. The average vertical displacement of SI of the resected pelvis was 6.951plusmn3.384 mm under vertical load of 500 Newton, which were 8.34 times compared with the intact pelvis. The average rotational angle of SI around the normal side femoral head of the resected pelvis was 4.055 plusmn 1.843deg, which were 12.5 times compared with the intact pelvis. Biomechanical model of type I resection of iliac tumor was established. Essential pelvic reconstruction must be introduced because of the serious instability of the pelvis after tumor resection. Digital marker tracing method with center-of-mass algorithm based on Image J software has good perspective for application in biomechanical research, which is a simple method with high precision.","PeriodicalId":6399,"journal":{"name":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","volume":"8 1","pages":"1836-1839"},"PeriodicalIF":0.0,"publicationDate":"2008-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89069818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rare Earth Waste Residue Absorbent Preparation and Applied Research 稀土废渣吸附剂的制备及应用研究

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.1095

Feng Xiujuan, Tao Tao

引用次数: 0

Effects of Ridge-Furrow Tillage on Soil Water and Crop Yield in Semiarid Region 垄沟耕作对半干旱区土壤水分和作物产量的影响

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.395

Muxing Liu, Yong-qin Ge

引用次数: 4

High-Resolution Trace Element Profiles in Calcium Carbonate Shells using LA-ICP-MS: An Environmental Proxy and Biogeochemical Cycling? LA-ICP-MS在碳酸钙壳中的高分辨率微量元素谱:环境代理和生物地球化学循环?

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.613

W. Guo, Shenghong Hu, Yue'e Peng

{"title":"High-Resolution Trace Element Profiles in Calcium Carbonate Shells using LA-ICP-MS: An Environmental Proxy and Biogeochemical Cycling?","authors":"W. Guo, Shenghong Hu, Yue'e Peng","doi":"10.1109/ICBBE.2008.613","DOIUrl":"https://doi.org/10.1109/ICBBE.2008.613","url":null,"abstract":"In this study we have used 193 ArF excimer laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), to produce a high resolution (32 mum) shells record of Mn, Zn, Sr, Ba, Mg, Cr, Cu, As, Cd, Co and Pb for the Periglypta reticulate and Tridacna gigas mussel shells from the Yalong bay, Hainan Island of China. Our synthesized CaCO3 co-precipitation multi-elements discs as the solid matrix- matched standards for calibration, and 42 bCa was selected as the internal standard to compensate the draft of signal. The results also demonstrated our co-precipitation matrix matched calibration method was more appropriate than the commercial NIST610/612 glass standards calibration method. Validation of the matrix- matched LA- ICP-MS technique indicated that the method provides accurate and reproducible (RSD<10%) analysis of trace elements in shells (0.01~3000 mug/g). The detection limits of this method for these elements were obtained in the range of 0.103 to 0.709 mug /g with correlation coefficients (R) better than 0.993. The proposed method was successfully applied to determine the trace elements in the two shells. The high-resolution trace element profiles could be as an environmental proxy and biogeochemical cycling for the area in which the fish or mussel lives.","PeriodicalId":6399,"journal":{"name":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","volume":"1 1","pages":"4480-4483"},"PeriodicalIF":0.0,"publicationDate":"2008-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91529873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Preparation and Anticoagulant Activity of Sulfated 6-Carboxychitosan 硫酸化6-羧基壳聚糖的制备及其抗凝血活性研究

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI: 10.1109/ICBBE.2008.241

Yuedong Yang, Yongguo Zhou, W.-L Hou, Yongguang Zhao, Y. Ren

引用次数: 0