Annika Ehmke, Jens Karl Wegener, Andreas Melfsen, Eberhard Hartung
{"title":"Optimizing exposure data collection for plant protection products: identifying ideal collectors with the fluorescent dye pyranine","authors":"Annika Ehmke, Jens Karl Wegener, Andreas Melfsen, Eberhard Hartung","doi":"10.1007/s00003-024-01498-5","DOIUrl":"10.1007/s00003-024-01498-5","url":null,"abstract":"<div><p>When approving plant protection products, exposure data are required for risk analysis. Exposure data can be collected for various exposure pathways, such as dermal, inhalation or ground sediment. For measuring exposures, pyranine, a fluorescent dye, and a collector can be used. However, the choice of collector material depends on the specific exposure pathway. This study aims to determine the most suitable collector, in combination with the tracer pyranine, for recording exposure through different pathways in practical trials.</p><p>Seven different collectors (Tyvek<sup>®</sup>, labels, plastic patches, paper patches, nylon filters, fibreglass filters, petri dishes) were subjected to laboratory and field tests to assess various quality parameters. Blank values, recovery rates, storability, and fluorescence degradation under UV-radiation were measured. Based on the results, a matrix was created summarizing which collector might be best suited to capture each exposure pathway. Almost all collectors demonstrated high recovery rates (Tyvek<sup>®</sup> 100%; labels 100%; plastic patches 100%; paper patches 100%; nylon filters 95%; fiberglass filters 60.9%) as well as good storability. Furthermore, all plastic-based collectors (labels, Tyvek<sup>®</sup>, plastic patches, petri dishes) showed a very good recovery rate above 95% when exposed to UV-radiation. However, nylon filters were not suitable for utilization under field conditions due to the rapid degradation of fluorescence under UV-radiation (recovery rate: 20–56%). Nevertheless, nylon filters showed stable recoveries under protected conditions and can be used to assess inhalation exposures under these conditions. Fibreglass filters, with recovery rates of 84–86%, were well-suited for testing inhalative exposures outdoors when a correction factor was applied. Tyvek<sup>®</sup> was the most suitable material for detecting total dermal exposure under field conditions. This is due to its quality characteristics and availability in a variety of sizes. Finally, petri dishes were ideal for collecting ground sediments.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"245 - 253"},"PeriodicalIF":1.4,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01498-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140037726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christopher Weidner, René Köppel, Regina Freyer, Petra Richl, Kathrin Lieske, Joachim Mankertz, Hans-Ulrich Waiblinger
{"title":"Development and validation of a multiplex real-time PCR method for screening genetically modified plants","authors":"Christopher Weidner, René Köppel, Regina Freyer, Petra Richl, Kathrin Lieske, Joachim Mankertz, Hans-Ulrich Waiblinger","doi":"10.1007/s00003-024-01499-4","DOIUrl":"10.1007/s00003-024-01499-4","url":null,"abstract":"<div><p>The diversity of genetically modified plants used for food and feed is increasing worldwide. For the detection and control of these products, efficient and reliable analytical tools are a prerequisite. This can be done by screening for specific DNA-elements and constructs characteristic of transgenic plants. In the past, numerous methods have already been published. However, several genetically modified plants are not covered by common screening methods. Here, a new qualitative triplex real-time polymerase chain reaction (PCR) method is presented, detecting two transgene flanking sequences and the transition between the Cassava Vein Mosaic Virus Promotor (P-CsVMV) and the phosphinothricin-N-acetyltransferase (pat) gene. These sequences are present in several transgenic plants and therefore, the described triplex method can be used as a screening tool to guide further analysis and increase the efficiency of the analysis strategy for GMO detection. The method is characterized by high specificity, sensitivity and robustness and is provided as a ring-trial validated method in the Official Collection of Methods according to the German Food and Feed Act.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"165 - 174"},"PeriodicalIF":1.4,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01499-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sophie Hedges, Ludovic Pelligand, Liwei Chen, Kelyn Seow, Thuy Thi Hoang, Huong Quynh Luu, Son Thi Thanh Dang, Ngoc Thi Pham, Hoa Thi Thanh Pham, Yeong Cheng Cheah, Yulan Wang, Dominique Hurtaud-Pessel, Anne Conan, Guillaume Fournié, Damer Blake, Fiona Tomley, Patricia L. Conway
{"title":"Antimicrobial residues in meat from chickens in Northeast Vietnam: analytical validation and pilot study for sampling optimisation","authors":"Sophie Hedges, Ludovic Pelligand, Liwei Chen, Kelyn Seow, Thuy Thi Hoang, Huong Quynh Luu, Son Thi Thanh Dang, Ngoc Thi Pham, Hoa Thi Thanh Pham, Yeong Cheng Cheah, Yulan Wang, Dominique Hurtaud-Pessel, Anne Conan, Guillaume Fournié, Damer Blake, Fiona Tomley, Patricia L. Conway","doi":"10.1007/s00003-024-01478-9","DOIUrl":"10.1007/s00003-024-01478-9","url":null,"abstract":"<div><p>Antimicrobials used in chicken farming for therapeutic and/or prophylactic purposes may result in unacceptable levels of edible residues, if withdrawal periods are not respected. To evaluate the risk in Vietnam, we validated an analytical method to detect antimicrobial residues from chicken meat samples and carried out a pilot cross-sectional study to identify optimal sampling strategies. A total of 45 raw meat samples were collected from 4 markets, 1 slaughterhouse and 4 farms (5 per site) in Northern Vietnam, between March and April 2021. Farmers were asked about antimicrobials used during sampled production cycles (5 chickens sampled per batch). Samples were analysed using liquid chromatography-tandem mass spectrometry for the presence of 68 antimicrobials at a pre-defined validation concentration. 7 compounds were identified from 4 classes (tetracyclines, sulphonamides, macrolides, and fluoroquinolones). In markets, where the source of sampled chickens was unknown, a diverse pool of residual antimicrobials was detected in 20% (4/20) of the meat samples. No residues were detected in samples from the slaughterhouse. No residues were detected in chickens from the one farm that reported using antimicrobials, whereas sulfadimethoxine, doxycycline and tilmicosin residues were identified from the other 3 farms reporting no antimicrobial use. The probability of detecting antimicrobial residues present in a flock based on sampling a single chicken was estimated at 0.93 (highest density interval 0.735–0.997). The preliminary results suggest a disparity between farmers’ reports on antimicrobial drug use and actual usage, and that the analysis of a single sample per farm has a high probability of detecting antimicrobial residues, if present.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"225 - 234"},"PeriodicalIF":1.4,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01478-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Staphylococcus aureus lytic bacteriophage: isolation and application evaluation","authors":"Fatmawati Lambuk, Nurzafirah Mazlan, Dalene Lesen, Elexson Nillian, Tze Young Thung","doi":"10.1007/s00003-024-01479-8","DOIUrl":"10.1007/s00003-024-01479-8","url":null,"abstract":"<div><p><i>Staphylococcus aureus</i> is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating <i>S. aureus</i> biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both <i>S. aureus</i> and methicillin-resistant <i>S. aureus</i> strains. Belonging to the <i>Myoviridae</i> family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm<sup>2</sup>, and 3.2 log CFU/cm<sup>2</sup>, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against <i>S. aureus</i>.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"235 - 243"},"PeriodicalIF":1.4,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oliver Meixner, Rainer Haas, Drini Imami, Iliriana Miftari
{"title":"Consumers preferences food safety perceptions for beef – a cross-country comparison in the wake of COVID-19","authors":"Oliver Meixner, Rainer Haas, Drini Imami, Iliriana Miftari","doi":"10.1007/s00003-024-01497-6","DOIUrl":"10.1007/s00003-024-01497-6","url":null,"abstract":"<div><p>Food safety is a pressing global concern, particularly in developing and emerging economies. The recent COVID-19 pandemic has further heightened consumer attention towards food safety, quality, and maintaining a healthy diet. This study provides insights into consumer awareness and perceptions related to food safety and the origin of beef products, with a specific focus on the impact of COVID-19. It draws comparisons between emerging economies, specifically Albania and Kosovo, where significant food safety challenges exist, and a highly developed economy, Austria. To assess consumer preferences, a discrete choice experiment was conducted, aiming to gauge the significance of various product attributes and consumers’ willingness to pay (WTP) for specific product features. The study’s findings revealed a strikingly strong sense of consumer patriotism across all surveyed countries. WTP estimates indicated a clear preference for domestically sourced food over imported alternatives. While the COVID-19 pandemic exerted a notable influence, its effects were generally contained. However, respondents from emerging economies perceived the pandemic’s impact to be more severe. Additionally, factors such as risk perception and risk acceptance played a role in shaping the importance of relevant product attributes in all the food markets examined in this study. Furthermore, distinct variations emerged between the countries concerning the importance of food safety standards. For instance, in Albania, the European Union (EU) food safety certificate holds higher utility compared to the national certificate, suggesting a potential lack of trust in national agencies. Notably, low trust in institutions, including those related to food safety, is a common issue in emerging and developing economies. This lack of trust, in turn, influences consumers’ perceptions of personal food safety, given that it is a credence attribute.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"187 - 198"},"PeriodicalIF":1.4,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01497-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adama Patrice Soubeiga, Dissinviel Stéphane Kpoda, Adama Sawadogo, Sibiri Sylvain Rouamba, Muller K. A. Compaoré, Asseto Belemlougri Somda, Sandrine Ouedraogo, Roukiatou Traoré, Désiré Nezien, Fulbert Nikiéma, Elie Kabre, Cheikna Zongo, Aly Savadogo
{"title":"Antimicrobial susceptibility and genotypic characterization of Escherichia coli isolated from foods controlled by the National Food Safety Agency in Burkina Faso","authors":"Adama Patrice Soubeiga, Dissinviel Stéphane Kpoda, Adama Sawadogo, Sibiri Sylvain Rouamba, Muller K. A. Compaoré, Asseto Belemlougri Somda, Sandrine Ouedraogo, Roukiatou Traoré, Désiré Nezien, Fulbert Nikiéma, Elie Kabre, Cheikna Zongo, Aly Savadogo","doi":"10.1007/s00003-024-01493-w","DOIUrl":"10.1007/s00003-024-01493-w","url":null,"abstract":"<div><p>This study was conducted to determine the antimicrobial resistance profiles of <i>Escherichia coli</i> isolated from food samples received at the National Food Safety Agency in Burkina Faso. A total of 155 isolates from dairy foods (29), fish-based dishes (7), mango juices (4), lettuces (35), RTE salads (21), sandwiches (32), and sesames (27) were included for testing with the Kirby-Bauer disk diffusion method. PCR targeting ampicillin (<i>bla</i><sub><i>TEM</i></sub>, <i>bla</i><sub><i>SHV</i></sub>, <i>temA</i>, and <i>temB</i>), tetracyclines [<i>tet(A)</i> and <i>tet(B)</i>], sulfamethoxazole (<i>sul1</i> and <i>sul2</i>), aminoglycosides (<i>StrA</i> and <i>aadA</i>) and quinolones (<i>GyrA</i>) resistance genes were performed to elucidate the genotypic resistance mechanism. Of the 155 isolates, 105 (67.7%) were resistant to at least one antimicrobial agent. Resistances to tetracycline (33.5%), ampicillin (32.9%), cefoxitin (18.7%), gentamycin (15.5%), amoxicillin-clavulanate acid (15.5%), nalidixic acid (12.9%), chloramphenicol (11.6%), trimethoprim-sulfamethoxazole (11.6%), and ciprofloxacin (8.4%) were observed. Multidrug resistance was recorded in 26.5% of the isolates. Antimicrobial resistance genes including <i>bla</i><sub><i>TEM</i></sub> (19/51, 37.3%), <i>bla</i><sub><i>SHV</i></sub> (19/51, 37.3%), <i>temB</i> (17/51, 33.3%), <i>tet(A)</i> (24/52, 46.2%), <i>tet(B)</i> (9/52, 17.3%), <i>sul1</i> (8/18, 44.4%), <i>sul2</i> (4/18, 22.2%), <i>aadA</i> (11/24, 45.83%) and <i>GyrA</i> (31/36, 86.1%) were detected. All <i>E. coli</i> isolates resistant to at least 2 antibiotics were positive for the class 1 integron gene (<i>intI1</i>). These findings raise concerns about food safety and public health and demonstrate the need for strict government control and continuous monitoring.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"213 - 223"},"PeriodicalIF":1.4,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139582408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Sasturain, Udo Blaschke, Franz Stauber, Tirso Oteyza, Jean-Pierre Huby, Sarah Adham, Neil Morgan, Anne-Kim Vinck, Michal Kicinski, Nicola J. Hewitt, Christian J. Kuster
{"title":"Minimizing operator exposure: field data analysis of three closed transfer systems for pesticide mixing and loading","authors":"Juan Sasturain, Udo Blaschke, Franz Stauber, Tirso Oteyza, Jean-Pierre Huby, Sarah Adham, Neil Morgan, Anne-Kim Vinck, Michal Kicinski, Nicola J. Hewitt, Christian J. Kuster","doi":"10.1007/s00003-023-01472-7","DOIUrl":"10.1007/s00003-023-01472-7","url":null,"abstract":"<div><p>Three Closed Transfer Systems (CTS) were evaluated for their reduction of operator exposure to plant protection products during mixing and loading: the inverted extraction systems, easyFlow M and easyconnect, and the probe extraction system, GoatThroat<sup>®</sup>. Exposure data were generated for gloves, hands, head, inner and outer body dosimeters generated in 4 European countries for high rate (Sorbitol) and low rate (Xylitol) surrogate products used by 12 operators. Exposure values were compared with predicted data using the Agricultural Operator Exposure Model as a benchmark for open pour loading (used in the EFSA Guidance). The CTS types conformed with the ISO 21191 standard for CTS performance, and their potential to significantly reduce operator exposure was confirmed in this study. There was no statistical difference in potential (naked) or actual (one layer of clothing) operator exposure using both inverted CTS types; allowing these data to be pooled. The inverted CTS types resulted in higher mean protection (> 98% potential exposure and > 95% actual exposure) than GoatThroat<sup>®</sup> (> 80% potential exposure and > 95% actual exposure). These data can be used to derive reduction factors that could be used in the AOEM model for calculations involving mitigation e.g., 0.05 for inverted CTS since they reduced exposure by > 95% and 0.3 for probe extraction CTS types. All 3 CTS significantly reduced operator exposure to products during M&L. These and future CTS types which comply with ISO 21191 standards could be considered as mitigation devices. These findings will help to build confidence of farmers to use CTS routinely, resulting in safer pesticide handling.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"143 - 153"},"PeriodicalIF":1.4,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-023-01472-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139458925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}