Listeria monocytogenes detection on food contact surfaces: suitability of different swab materials

IF 1.4 3区农林科学 Q4 FOOD SCIENCE & TECHNOLOGY

Journal of Consumer Protection and Food Safety Pub Date : 2023-09-01 DOI:10.1007/s00003-023-01454-9

Nadja Hillig, Ahmad Hamedy, Martin Koethe

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引用次数: 0

Abstract

The high binding capacity of Listeria monocytogenes to food contact surfaces increases the risk of cross-contamination in food. In addition to appropriate cleaning and disinfection procedures, a suitable sampling plan and technique for the earliest possible detection are necessary for prevention. This paper evaluates the sensitivity of 3 swab materials (cotton, viscose and nylon-flocked) for the qualitative and quantitative detection of L. monocytogenes on food contact surfaces (100 cm²). A L. monocytogenes cocktail of 3 serotypes (IIa, IIb and IVb) was applied to stainless steel, polyvinyl chloride, polytetrafluoroethylene and high-density polyethylene surfaces at a concentration of approx. 1.0 × 10¹-1.0 × 10² CFU/100 cm² and approx. 4.0 × 10⁴ CFU/100 cm². The surfaces were sampled after 15 min of incubation by 3 different swabs using the double-swab technique, and then stored for 4 and 24 h until processing. The results of the qualitative and quantitative tests showed a few statistically significant differences in the detectability of L. monocytogenes by different swab materials, which implies that the detection rate of L. monocytogenes on a certain food contact surfaces can be increased by using the respective most suitable swab.

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食品接触表面单核增生李斯特菌检测:不同拭子材料的适用性

单核细胞增生李斯特菌对食品接触面的高结合能力增加了食品中交叉污染的风险。除了适当的清洁和消毒程序外，适当的取样计划和尽早发现的技术是预防的必要条件。本文评价了3种棉签材料(棉、粘胶和尼龙绒)对食品接触表面(100 cm2)单核细胞增生乳杆菌的定性和定量检测的灵敏度。将3种血清型(IIa、IIb和IVb)的单核细胞增生乳杆菌混合物以约为浓度分别施用于不锈钢、聚氯乙烯、聚四氟乙烯和高密度聚乙烯表面。1.0 × 101-1.0 × 102 CFU/100 cm2。4.0 × 104 CFU/100 cm2。用3种不同的拭子(双拭子技术)孵育15 min后对表面取样，然后分别保存4和24 h待处理。定性和定量试验结果显示，不同拭子材料对单核增生乳杆菌的检出率有统计学差异，说明使用各自最合适的拭子可提高特定食品接触面上单核增生乳杆菌的检出率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Consumer Protection and Food Safety Veterinary-Food Animals

CiteScore

3.70

自引率

4.20%

发文量

审稿时长

>12 weeks

期刊介绍： The JCF publishes peer-reviewed original Research Articles and Opinions that are of direct importance to Food and Feed Safety. This includes Food Packaging, Consumer Products as well as Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering. All peer-reviewed articles that are published should be devoted to improve Consumer Health Protection. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of Food and Feed Safety issues on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of Food and Feed Safety and Consumer Health Protection. Manuscripts – preferably written in English but also in German – are published as Research Articles, Reviews, Methods and Short Communications and should cover aspects including, but not limited to: · Factors influencing Food and Feed Safety · Factors influencing Consumer Health Protection · Factors influencing Consumer Behavior · Exposure science related to Risk Assessment and Risk Management · Regulatory aspects related to Food and Feed Safety, Food Packaging, Consumer Products, Plant Protection Products, Food Microbiology, Veterinary Drugs, Animal Welfare and Genetic Engineering · Analytical methods and method validation related to food control and food processing. The JCF also presents important News, as well as Announcements and Reports about administrative surveillance.