Journal of Applied Spectroscopy最新文献

{"title":"Evaluation of Stability Constant for Vitamin B1 Depending on Stoichiometric Curves by Visible Spectroscopy","authors":"Azhar S. Hamody,&nbsp;Nahla A. Alassaf,&nbsp;Resala A. Saleh,&nbsp;Faeza H. Zankanah,&nbsp;Sarmad B. Dikran","doi":"10.1007/s10812-025-01860-x","DOIUrl":"10.1007/s10812-025-01860-x","url":null,"abstract":"<p>The measurement of vitamin B₁ in pure and pharmaceutical formulations was proposed by using a straightforward and sensitive spectrophotometric approach. Sulfacetamide (SFA) is diazotized, then coupled with vitamin B₁ in alkaline media to produce a colored azo dye complex with a stability constant of 5.597 × 10⁵ L/mol. The product is stable, with a maximum absorption wavelength of 489.5 nm, molar absorptivity of 10108 L/mol∙cm, Sandell's sensitivity of 0.0334 μg/cm², detection limit of 0.0135 μg/mL, and Beer's law being observed over the concentration range of 0.2–20.0 μg/mL. The stability constant and stoichiometry of the produced azo dye were calculated using the continuous variation (Job's) and mole ratio methods. The suggested approach was used successfully in the determination of vitamin B₁ in its pharmaceutical formulations.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1353 - 1361"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Aprepitant Via Charge Transfer Complexation Reactions Through Visible Spectrophotometric Methods","authors":"M. Chaitanya,&nbsp;T. Hemant Kumar,&nbsp;S. Koduru,&nbsp;S. Kalepu","doi":"10.1007/s10812-025-01868-3","DOIUrl":"10.1007/s10812-025-01868-3","url":null,"abstract":"<p>Two spectrophotometric approaches based on charge transfer complexation processes were developed, validated, and deployed for the detection of aprepitants in pharmaceutical dosages. In method A, the aprepitant reacts para chloranilic acid (p-CA) in acetonitrile, resulting in the formation of a purple-colored product. This product exhibited a maximum absorbance at a wavelength of 524 nm. In technique B, the combination of aprepitant and picric acid (PA) in chloroform results in the generation of chromophores having yellow coloration with a peak absorbance at 415 nm. Every experimental parameter was thoroughly examined and fine-tuned to optimize color development. The validation properties of the recommended spectrophotometric approaches, including linearity, range, precision, accuracy, specificity, robustness, detection, and quantification restrictions, were eff ectively evaluated. Within the concentration range of 10–60 μg/mL, both approaches exhibited a linear relationship with high correlation coefficient values. The suggestions that the processes for quantifying aprepitants in dosage form are straightforward, rapid, precise, accurate, and cost-effective.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1418 - 1427"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-Infrared-Spectroscopy-Based Method for Identifying Single and Multiple Vegetable Protein Adulterants in Whey Protein","authors":"Yuduan Lin,&nbsp;Honghao Cai,&nbsp;Shihao Lin,&nbsp;Hui Ni","doi":"10.1007/s10812-025-01863-8","DOIUrl":"10.1007/s10812-025-01863-8","url":null,"abstract":"<p>With the rising popularity of whey protein as a dietary supplement, ensuring its quality has become imperative for consumer protection. Unscrupulous merchants sometimes adulterate whey protein with inexpensive vegetable protein to boost profits. Despite the criticality of this concern, reliable studies and relevant practical detection methods are currently limited. To fill this gap, this study adopted an integrated technique combining mid-infrared spectroscopy with machine learning to rapidly and accurately identify both single and multiple vegetable protein adulterants in whey protein. First, various recognition models were trained using AdaBoost-support vector classification (AdaBoost-SVC), AdaBoost-decision tree, K-nearest neighbor, SVC, and Gaussian Naive Bayes. Ten-fold cross-validation was subsequently used to determine the optimal spectra pre-processing combination, which included standard normal variate, first derivative, and Savitzky–Golay smoothing. Feature selection was then performed using the successive projection algorithm, principal component analysis, genetic algorithm (GA), and interval partial least squares with GA (iPLS-GA). The classification results revealed that the iPLS-GA–AdaBoost-SVC achieved the best performance on both the training and prediction sets, demonstrating the ability of the iPLS-GA to improve model stability and robustness. Overall, our findings underscore the potential applicability of the proposed method as an accurate and practical tool for improving the quality control of whey protein.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1378 - 1386"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrophotometric and Liquid Chromatographic Methods for Quantification of Antibacterial Fluorescent Dye (Phloxine B) in Dental Disclosing Tablets and Toothpaste Samples","authors":"Ali F. Alghamdi","doi":"10.1007/s10812-025-01865-6","DOIUrl":"10.1007/s10812-025-01865-6","url":null,"abstract":"<p>Double beam UV-Vis spectrophotometry and high-performance liquid chromatography (HPLC) techniques have been used to determine the antibacterial fluorescent phloxine B dye in dental preparations. In the visible wavelength range of 400 to 800 nm, the spectrophotometric signal was obtained at λ_max = 539 nm. The HPLC process used C-18 (5 μm) with a UV detector at 254 nm, 1.5 mL/min flow rate, 0.635 min retention time, and water, acetonitrile, and 2-propanol as the mobile phase. Several parameters have been used to analyze the performance of the HPLC and double-beam UV-Vis spectrophotometry apparatuses, including stability, detection and quantification limits, calibration curves, and repeatability. The study focused on the repeatability of spectrophotometric and HPLC procedures for 8 × 10^–6 and 5 × 10^–6 mol/L of phloxine B. The results showed that the standard deviation (STD) for ten spectrophotometric measurements and eight HPLC measurements, respectively, was ± 0.0004 and ± 2.487 with relative standard deviation (RSD%) of 0.048 and 1.05%. Throughout 90 min of the analysis period, the spectrophotometric and HPLC signals for 8 × 10^–6 and 5 × 10^–6 mol/L of phloxine B were shown to be exceptionally stable. The ranges of the calibration curves were 1 × 10^-6–2 × 10^–5 mol/L for the spectrophotometry and 1 × 10^–5–1 × 10^–4 mol/L for HPLC. Within the concentration ranges under consideration, they were assigned linear relations with correlation coefficients (<i>r</i>²) of 0.9995 for spectrophotometric and 0.99 for HPLC measurements. The detection limits for phloxine B were found to be 1.24 × 10^–8 mol/L (0.0103 ppm) and 8.25 × 10^–7 mol/L (0.068 ppm), respectively, using double-beam UV-visible spectrophotometry and HPLC methods. These techniques use recovery rates ranging from 95 to 111% to determine the phloxine B dye that has been spiked in disclosing tablets and toothpaste samples.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1394 - 1403"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and pH-Sensing of Simple but Effective Ruthenium(II) Polypyridine Complexes: Potential Lysosome Probes","authors":"Kexin Liu,&nbsp;Miao Tian,&nbsp;Xingfang Li,&nbsp;Xiu Sun","doi":"10.1007/s10812-025-01864-7","DOIUrl":"10.1007/s10812-025-01864-7","url":null,"abstract":"<p>The pH optical sensors work on the principle of pH-related changes in the absorption or emission of a probe. They have generated much interest during the past decades due to their wide applications in fields such as analytical chemistry, biochemistry, clinical medical diagnosis, and environmental monitoring. Two ruthenium(II) polypyridine complexes, [Ru(bpy)₂(hipp)]²⁺ (Ru1) and [Ru(bpy)₂(dcipp)]²⁺ (Ru2), were chosen as pH probes. Here, bpy denotes 2,2-bipyridine, hipp denotes 2-(1H-imidazo [4,5-f][1,10]phenanthrolin-2-yl)phenol, and dcipp denotes 2,4-dichloro-6-(1H-imidazo[4,5-f] [1,10]phenthrolin-2-yl)phenol, respectively. The pH effects on the absorption and emission spectra of Ru1 and Ru2 were investigated by pH titrations. Ru1 and Ru2 were found to be pH-sensitive and revealed different ionization constants, suggesting their potential use as optical pH sensors and applications in environmental monitoring.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1387 - 1393"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Photoluminescence Studies of Eu3+-Activated Ca3Al2O6 Phosphor","authors":"K. Koteswara Rao,&nbsp;M. C. Rao,&nbsp;Vikas Dubey","doi":"10.1007/s10812-025-01857-6","DOIUrl":"10.1007/s10812-025-01857-6","url":null,"abstract":"<p>The present paper reports the synthesis and photoluminescence (PL) properties of Eu³⁺-activated calcium aluminate (Ca₃Al₂O₆) phosphors. The samples were prepared by a conventional solid-state reaction method with different concentrations of Eu³⁺ ions, which was most suitable for large-scale production. The prepared phosphor sample was characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FTIR), PL, and CIE chromaticity coordinate techniques. The XRD pattern indicated that the sample was mono-phased and crystallizes in a cubic structure. The crystallite size was found to be nearly 82 nm and the SEM images showed the formation of micro-rods at low resolution and at high resolution exhibited a cube-like morphology. The FTIR studies confirmed the formation of Ca₃Al₂O₆:Eu³⁺ phosphor. The PL excitation spectra found at 271 and 396 nm and the PL emission were observed in the range 500-750 nm for the Ca₃Al₂O₆ phosphor doped with Eu³⁺ ions and sharp peaks were found at 595, 616, 621, and 651 nm with high intensity. The present phosphor can act as a single host for red-orange light emission in display devices.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1332 - 1337"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Photocatalytic Performance of PSII–rGO Composite Towards Methylene Orange Degradation","authors":"Shixun Zheng,&nbsp;Songyang Han,&nbsp;Siyuan Bi,&nbsp;Yantong Wang","doi":"10.1007/s10812-025-01866-5","DOIUrl":"10.1007/s10812-025-01866-5","url":null,"abstract":"<p>A PSII–rGO nanocomposite photocatalyst utilizing graphene oxide and PSII precursor was prepared in this study. In this proposed facile approach, PSII dimers were uniformly distributed on the reduced graphene oxide (rGO) sheets. The retained oxygen functional groups (OFGs) on rGO planes played a significant role in anchoring the PSII dimers. The samples were thoroughly characterized by X-ray diffraction (XRD), Raman spectroscopy, ultraviolet-visible (UVvis) spectroscopy, and photoluminescence spectra (PL) analysis. The photocatalytic activity of PSII–rGO composites was further investigated by photodegrading methyl orange (MO). As a result, the prepared PSII–rGO photocatalyst exhibited a high absorptivity towards MO and efficient charge separation properties. The efficiency of the PSII–rGO composites (61%) towards the degradation of MO was significantly higher compared to pure PSII (25%), showing a 36% increase within 5 h under visible light. Finally, the corresponding mechanism of this enhancement was proposed and discussed in detail. After receiving photoelectrons from PSII, the rGO plane effectively transferred them to pollutants, thereby achieving a high photocatalytic degradation efficiency. The introduction of rGO improved the photocatalysis and sensing properties of PSII by facilitating rapid electron/charge division. Overall, the prepared PSII–rGO nanocomposite shows great potential for practical applications.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1404 - 1410"},"PeriodicalIF":0.8,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybridization Chain Reaction-Based Label-Free Colorimetric Sensor for Detection of Cancer Marker p53 Gene","authors":"S. Yang,&nbsp;L. Ren,&nbsp;Z. Qin,&nbsp;P. Zhang,&nbsp;Q. Zhang,&nbsp;J. Zhang,&nbsp;L. Jiang","doi":"10.1007/s10812-025-01869-2","DOIUrl":"10.1007/s10812-025-01869-2","url":null,"abstract":"<p>The p53 gene is an important tumour suppressor gene, which has an important impact on the early diagnosis of cancer. Here, inspired by the fact that single-stranded DNA (ss-DNA) can be nonspecifically adsorbed on the surface of nanogold, we report on a sensitive and cost-effective new method to detect the p53 gene by combining hybridization chain reaction (HCR) with gold nanoparticles. The long double helix structure generated by HCR could not be adsorbed on the nanogold surface after the addition of the p53 gene. When an appropriate amount of Na⁺ is introduced into the solution, the nanogold aggregates and the color of the solution changes from red to blueviolet. The sensor has a high sensitivity with a detection limit of 2 nM visible to the naked eye and a quantitative detection limit of 0.2 nM using a UV-visible spectrophotometer. Notably, by combining fluorescence spectroscopy and gel electrophoresis, the science and specificity of p53 gene-induced HCR is systematically validated. The use of an enzyme-free, label-free colorimetric method to detect the p53 gene greatly reduces the complexity and cost of the experiment. This study has a broad market application prospect and provides a new method for early mass screening of cancer genes.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1428 - 1438"},"PeriodicalIF":0.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural, Optical, and Photoelectric Properties of Silicon Implanted with Indium and Antimony Ions and Subjected to Pulsed Annealing","authors":"R. I. Batalov,&nbsp;V. V. Bazarov,&nbsp;V. I. Nuzhdin,&nbsp;V. F. Valeev,&nbsp;H. A. Novikov,&nbsp;V. A. Shustov,&nbsp;K. N. Galkin,&nbsp;I. B. Chistokhin,&nbsp;F. F. Komarov,&nbsp;O. V. Milchanin,&nbsp;I. N. Parkhomenko","doi":"10.1007/s10812-025-01841-0","DOIUrl":"10.1007/s10812-025-01841-0","url":null,"abstract":"<p>Si single crystal was sequentially implanted with high doses (2∙1016 cm^-2) of In⁺ and Sb⁺ ions with an energy of 30 keV in order to synthesize a layer of narrow-gap indium antimonide (InSb) in its near-surface region. Implanted Si:(In + Sb) layers in the liquid phase were annealed with a powerful pulsed (~100 ns) ion beam (C⁺/H⁺) with an energy of 300 keV and a pulse-energy density of 1.0 J/cm². Calculation of the total depth profile of the concentration of implanted In and Sb atoms taking into account ion sputtering showed their maximum concentration of 40 at.% at a depth of ~20 nm. Segregation of impurity atoms to the Si surface because of pulsed annealing was detected using the Rutherford backscattering of He⁺ ions. X-ray diffraction spectra in grazing beams and Raman light scattering indicated an InSb phase with a tensile strain level of 0.6–0.7% formed. The electron concentration in the layer (2∙1020 cm^-3) due to the Sb donor impurity was estimated using optical IR spectra. An intense absorption band at 3.85 μm was shown to form. Photoresponse measurements on a diode mesa-structure at 300 K showed a shift in the photosensitivity edge to 1240 nm as compared to a standard FD-24 Si photodiode.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1225 - 1232"},"PeriodicalIF":0.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the Oxidative Annealing Temperature on the Structural and Optical Characteristics of Tin Oxide Films","authors":"V. K. Ksenevich,&nbsp;V. A. Dorosinets,&nbsp;M. A. Samarina,&nbsp;D. V. Adamchuk,&nbsp;G. Abdurakhmanov,&nbsp;H. Liu","doi":"10.1007/s10812-025-01842-z","DOIUrl":"10.1007/s10812-025-01842-z","url":null,"abstract":"<p>Results are given for a study of the crystal structure and optical characteristics of disordered tin oxide films. The synthesis of these films was carried out by means of magnetron sputtering of a tin target on a glass substrate followed by two-stage annealing in the air. The microstructure of the tin oxide films was analyzed by x-ray diffraction and Raman spectroscopy. Transmission spectra of the samples were taken in the wavelength range λ = 200–3000 nm. The optical constants of the thin tin oxide films, namely, the refractive index n and absorption coefficient α, relative to wavelength were determined using the envelope method. Feasibility was shown for the preparation of tin oxide films with control of the optical parameters (absorption coefficient α up to 82% in the visible range of the electromagnetic spectrum, refractive index n in the range 2–2.6, and Tauc optical gap in the range 2.62–3.46 eV) by adjusting the temperature in the second stage of the oxidative annealing process in the range 325–475oC.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"91 6","pages":"1233 - 1239"},"PeriodicalIF":0.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}