Journal of Applied Spectroscopy最新文献

{"title":"Synthesis, Characterization, and Photoluminescence Properties of Pr3+-Doped SrAl2B2O7","authors":"İlhan Pekgözlü","doi":"10.1007/s10812-026-02081-6","DOIUrl":"10.1007/s10812-026-02081-6","url":null,"abstract":"<p>Sr_1–<i>x</i>Pr_<i>x</i>Al₂B₂O₇ (0.005 ≤ <i>x</i> ≤ 0.04) red-emitting phosphors were prepared by firing in air at 950°C for 6 h. Using the XRD technique, the structural characterization of the prepared materials was examined. Additionally, FTIR was used to examine the Al–O and B–O bond characteristics in the crystal structure. Finally, using a spectrofluorometer, the luminescence property of Sr_1–<i>x</i>Pr_<i>x</i>Al₂B₂O₇ (0.005 ≤ <i>x</i> ≤ 0.04) was thoroughly examined at ambient temperature. The prepared SrAl₂B₂O₇ materials with varying Pr³⁺ doping emitted radiation at three wavelengths in the red region (609, 622, and 645 nm) when exposed to 469-nm light.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"254 - 257"},"PeriodicalIF":1.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Protect–Deprotect” Based Fluorescent Probe for the Recognition of Norepinephrine","authors":"Wei Gao,&nbsp;Hao-Na Zhang,&nbsp;Wen-Ling Wang,&nbsp;Yan-Li Chen,&nbsp;Qingming Wang","doi":"10.1007/s10812-026-02079-0","DOIUrl":"10.1007/s10812-026-02079-0","url":null,"abstract":"<p>Research has shown a significant correlation between severe depression and reduced levels norepinephrine (NE) in brain tissue. Therefore, designing NE-specific fluorescent probes is highly challenging. We adopted a “protect-deprotect” strategy in which the 7-hydroxy-4-methylcoumarin fluorescence group was protected by a carbonic ester that linked to the departing thiophenol group. The probe then underwent two nucleophilic additions and eliminations by NE, which released the fluorophore and enabled specific fluorescence detection of NE with sensitivity and selectivity. This recognition mechanism was verified using the high-resolution mass-spectrometry method.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"238 - 242"},"PeriodicalIF":1.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Antibacterial and Anticancer Properties of Green Zinc Oxide Nanoparticles Synthesized from Spent Camellia sinensis (Tea) Leaves","authors":"M. R. Islam,&nbsp;A. Arora,&nbsp;I. Laskar,&nbsp;R. Saha","doi":"10.1007/s10812-026-02080-7","DOIUrl":"10.1007/s10812-026-02080-7","url":null,"abstract":"<p>Plant-based food waste contains high concentrations of phytochemicals, rendering it an appealing candidate for the eco-friendly production of metal nanoparticles. In this study, zinc oxide nanoparticles (ZnO NPs) were prepared from spent <i>C. sinensis</i> (tea) leaves, serving as both a reducing and a stabilizing agent. Zinc acetate dihydrate [(CH₃COO)₂Zn·2H₂O] was used as a precursor in the spent <i>C. sinensis</i> leaf extracts for the NPs synthesis. The physicochemical characterization of ZnO NPs was characterized using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectrophotometer (EDX), X-ray diffraction (XRD), photoluminescence spectroscopy (PL), and dynamic light scattering (DLS). The synthesized ZnO NPs displayed a strong UV-Vis absorption peak at 370 nm, and a wurtzite hexagonal structure with an average crystalline size of 56 nm, as confirmed by XRD. The FESEM confirmed a spherical morphology, while EDX analysis indicated zinc as the predominant element, followed by oxygen. The antibacterial efficacy of ZnO NPs was assessed against both Gram-negative and Gram-positive pathogens, exhibiting optimal effectiveness against Streptococcus pyogenes and Staphylococcus aureus. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that ZnO NPs exhibited concentration-dependent anticancer activity against HeLa cells, with an IC₅₀ of approximately 33.5 μM. Live/dead imaging confirmed a 4.6-fold and 3.5-fold increase in dead cells at IC₅₀ concentrations after 24 and 48 h, respectively. Furthermore, treatment with ZnO NPs significantly elevated reactive oxygen species (ROS) production, indicating that their cytotoxicity is mediated through ROS generation and its lethal effects. Our findings suggest that ZnO NPs made from spent <i>C. sinensis</i> leaf extracts could have antibacterial and anticancer properties.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"243 - 253"},"PeriodicalIF":1.0,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Multicenter Interactions in Formation of Spectral–Luminescent Characteristics of 5,15-Diphenylporphyrin Derivatives","authors":"L. L. Gladkov,&nbsp;D. V. Klenitsky,&nbsp;S. A. Syrbu,&nbsp;M. M. Kruk","doi":"10.1007/s10812-026-02053-w","DOIUrl":"10.1007/s10812-026-02053-w","url":null,"abstract":"<p>The peculiarities of forming the spectral–luminescent characteristics of 5,15-diphenylporphyrin derivatives with various peripheral substitution architectures have been studied. Molecular conformations of derivatives with various peripheral substituents in C_b- and C_m-positions of the tetrapyrrole macrocycle were optimized using a density functional method. Substitution of two diametrically opposed C_m-atoms was shown to lead to elongation of the macrocycle in the direction of substitution regardless of the nature of the C_b-substitution. Dodecasubstituted derivatives exhibited a saddle-shaped distortion of the macrocycle. The fluorescence quantum yield of 5,15-diphenylporphyrin and its C_b-alkylated derivatives decreased as compared to that of 5,10,15,20-tetrapheny lporphyrin. Introduction of nitro groups and bromine atoms in the para-position of the phenyl fragments led to enhancement and quenching of the fluorescence, respectively. It has been established that the addition of two nitro groups in the C_m-positions of the 5,15-diphenylporphyrin macrocycle led to substantial bathochromic shifts of the absorption and fluorescence spectra with 100-fold fluorescence quenching due to significant gain of nonradiative deactivation of the S₁-state upon forming the saddle-shaped conformer.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"11 - 17"},"PeriodicalIF":1.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147558974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Nitrogen-Doped Carbon Quantum Dots and Analysis and Detection Of Vitamin B2","authors":"Hui Yang,&nbsp;Xiucheng Yang","doi":"10.1007/s10812-026-02073-6","DOIUrl":"10.1007/s10812-026-02073-6","url":null,"abstract":"<p>This paper focuses on the preparation of Nitrogen-doped carbon quantum dots (N-CQDs) from citric acid and urea, and their application in the detection of vitamin B2. Citric acid was used as the carbon source and urea as the nitrogen source. N-CQDs were prepared by heating, after which they displayed excellent fluorescence performance and stability. The structure of the sample was then characterized using UV-visible spectrophotometry, fluorospectrophotometry, and Fourier transform infrared spectroscopy. Optimal pH, ultrasonic reaction time, and vitamin B2 concentration for the N-CQDs were studied and optimized. Under optimal conditions (50 μL of N-CQDs, 2 mL of buffer solution with pH 4.0, and a reaction time of 30 min), the analysis and detection using fluorospectrophotometry demonstrated that vitamin B2 caused significant fluorescence quenching of the prepared N-CQDs. With the standard recovery rate above 80%, these N-CQDs can serve as fluorescence probes for the analysis and detection of vitamin B2 in actual food.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"178 - 186"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic Spectrophotometric Quantification of Vitamin H in Pristine and Drug Formulations in Micellar Medium","authors":"Abhishek Srivastava,&nbsp;Ajaya Bhattarai,&nbsp;Pradeep Kumar Pandey,&nbsp;Neetu Srivastava","doi":"10.1007/s10812-026-02074-5","DOIUrl":"10.1007/s10812-026-02074-5","url":null,"abstract":"<p>A simple, effective, and accurate visible-spectroscopic technique has been established to evaluate vitamin H (VIT-H) in medicinal samples as well as in its pure form. The method depends on the inhibitory influence of VIT-H on the Hg²⁺-facilitated ligand substitution (LS) reaction between phenylhydrazine (PHZ) and [Fe(CN)6]4–. The strategy involves substituting CN^– with PHZ in [Fe(CN)₆]^4–, resulting in the formation of a [Fe(CN)₅ PHZ]^3– complex. The VIT-H can be quantified between 0.017 and 1.710 μg/mL by monitoring the absorbance of the complex at 488 nm during the process. The established method enables the detection of VIT-H at concentrations as low as 0.163 μg/mL. Recuperation experiments confirmed the precision and accuracy of the method for VIT-H quantification. The proposed methodology has been successfully employed for the assessment of VIT-H in pure samples and diverse pharmaceuticals, showcasing remarkable accuracy and precision. The results corresponded with the prescribed analytical protocol. The suggested methodology remains unaffected by the excipients typically employed in medications.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"187 - 196"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized Low-Cost Liquid‒Liquid Extraction Mode for the Removal and Spectrophotometric Determination of Cr(VI) from Polluted Water","authors":"Ashwini V. Sadlapurkar,&nbsp;Umesh B. Barache,&nbsp;Abdul B. Shaikh,&nbsp;Shashikant H. Gaikwad,&nbsp;Anita A. Ghare,&nbsp;Arati V. Diwate,&nbsp;Ganesh S. Kamble,&nbsp;Tukaram N. Lokhande","doi":"10.1007/s10812-026-02076-3","DOIUrl":"10.1007/s10812-026-02076-3","url":null,"abstract":"<p>Chromium(VI), considered one of the most hazardous elements, directly affects human health and the environment. Therefore, in this study, a new approach is devised for liquid‒liquid extraction and spectrophotometric measurement of hexavalent chromium by employing a novel spectrophotometric reagent, where by 2-chlorobenzaldehyde Thiocarbohydrazone in dichloroethane is employed as a complexing reagent for chromium(VI) in the presence of potassium iodide, resulting in a yellow complex at room temperature. This approach offers a considerable benefit, being a straightforward procedure that does not require any extra solvent purification or preconcentration. The ternary [Cr(VI)–2CBTCH–iodide] complex was quantitatively recovered in dichloroethane from 3.5 mol/L hydrochloric acid medium. The maximum absorbance at λ_max = 415 nm was reached, and the sample was stable for 72 h. The method showed excellent analytical response, with a limit of detection of 0.32 μg/mL, a wide working range of up to 10.25 μg/mL, and reasonable accuracy (RSD &lt; 2%, <i>n</i> = 5). The molar absorptivity and Sandell sensitivity of the ternary complex are 0.3535 × 10⁴ L/mol∙cm and 0.0147 μg/cm, respectively, whereas 2.496 is the enrichment factor. Using the log‒log plot method, the [Cr(VI)–2CBTCH–iodide] complex composition was verified to be 1:2:2. There are no noticeable consequences of possibly interacting ions. Chromium(VI) was successfully extracted and determined simultaneously from alloy samples, contaminated water, and synthetic mixtures via this technology. The applied method has numerous advantages, including simplicity, low cost, ease of operation, rapid detection, low ligand consumption, and high sensitivity. The sensitivity of the analytical method was proven by selecting appropriate experimental conditions.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"205 - 215"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Stress-Induced Generation of Reactive Oxygen Species in Phalaenopsis × hybridium Blume Protocorms Using a Redox-Sensitive Dihydroethidium Fluorescent Probe","authors":"M. A. Chernysh,&nbsp;V. S. Matskevich,&nbsp;S. N. Zvonarev,&nbsp;A. F. Bakhmetova,&nbsp;G. N. Smolikova,&nbsp;S. S. Medvedev,&nbsp;V. V. Demidchik","doi":"10.1007/s10812-026-02061-w","DOIUrl":"10.1007/s10812-026-02061-w","url":null,"abstract":"<p>Methods were developed for analyzing and studying the most important stress responses of protocorm epidermal cells of Phalaenopsis × hybridium Blume involving the synthesis of reactive oxygen species (ROS), in particular, the superoxide radical anion <span>(left({O}_{2}^{bullet -}right))</span>. Epifluorescence microscopy was adapted for this purpose in combination with a dihydroethidium fluorescent probe and a Nikon FITC B-2E/C narrow-band fluorescent filter cube. This approach made it possible to avoid artifacts arising from the intense red fluorescence generated when ethidium (a product of the nonspecific oxidation of dihydroethidium) binds to DNA and to record the green signal of 2-hydroxyethidium (2-OH-E⁺) specific for the reaction with <span>({O}_{2}^{bullet -})</span>. Using the developed methods, ROS generation in Orchid protocorms subjected to mechanical damage as well as osmotic and salt stress was analyzed for the first time. The abovementioned factors were shown to induce <span>({O}_{2}^{bullet -})</span> generation in the protocorm epidermis identified by enhanced 2-OH-E⁺ fluorescence. The addition of enzymatic and low-molecular-weight antioxidants such as superoxide dismutase (SOD), catalase, dimethyl sulfoxide (DMSO), and thiourea suppressed the stress-induced ROS synthesis in protocorms. The greatest decrease in the fluorescence was seen in the presence of SOD and thiourea, indicating specificity of the reaction of dihydroethidium with <span>({O}_{2}^{bullet -})</span>. These approaches can be applied to the analysis of the early stages of the oxidative imbalance arising in the cells of Orchidaceae and other higher plants due to the effect of abiotic stress factors.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"77 - 85"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Annealing in an Atomic Oxygen Atmosphere on Photoluminescent and Electrical Properties of Hydrothermal ZnO Single Crystals","authors":"I. V. Rogozin,&nbsp;O. V. Rogozina","doi":"10.1007/s10812-026-02057-6","DOIUrl":"10.1007/s10812-026-02057-6","url":null,"abstract":"<p>The effect of annealing in an atomic oxygen atmosphere on the photoluminescent and electrical properties of highquality ZnO single crystals grown by the hydrothermal method was studied. The spectrum of as-prepared ZnO samples exhibited a series of narrow lines in the UV region and long-wavelength emission peaking at 510 nm. The nature of the observed bands was discussed. Hall effect measurements indicated that the dominant donor in as-prepared ZnO samples had ionization energy E_D = 67 meV and concentration N_D = 3.6∙10¹⁸ cm^–3. The ND value decreased to 3.7∙1017 cm^–3, while the E_D value did not change after heat treatment in an atomic oxygen atmosphere at 600–700°C for 1 h. A ZnO single crystal annealed at 800°C showed p-type conductivity with an acceptor concentration N_A = 2.7∙10¹⁶ cm^–3.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"42 - 48"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Aluminum Foil as a Sers-Active Substrate in the Study of Artistic Pigments","authors":"E. V. Klyachkovskaya","doi":"10.1007/s10812-026-02052-x","DOIUrl":"10.1007/s10812-026-02052-x","url":null,"abstract":"<p>The use of aluminum foil as a low-cost and promising SERS-active substrate is proposed. Aluminum foil demonstrated plasmonic properties comparable to and in some cases even superior to those of traditional SERS-active substrates based on gold nanoparticles in model experiments with various artistic pigments. The inorganic pigments used in samples taken from Southern Landscape supposedly by A. Kuprin and The Virgin and Child from the Church of St. John the Baptist in Borovtsy were identified. The time limits of their creation were defined.</p>","PeriodicalId":609,"journal":{"name":"Journal of Applied Spectroscopy","volume":"93 1","pages":"1 - 10"},"PeriodicalIF":1.0,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147561893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}