Development and Validation of UV Spectrophotometric Method for the Estimation of Naringin in Bulk Followed by Its Solubility Study

Abstract

The aim of this study was to develop an ultraviolet spectrophotometry-based analysis method for determining naringin in bulk solutions, as well as to validate it for linearity, precision, accuracy, LOD, and LOQ. The study devised and validated a procedure to accurately detect naringin in a bulk solution using a comparatively economical, more reliable, and efficient technique. We are expecting similar results with comparable accuracy to the existing expensive techniques but with minimized risks. We utilized pure naringin (API) and methanol (solvent) to develop the method and further used water, isopropyl alcohol, chloroform, ethyl acetate, butan-1-ol, mustard oil, neem oil, citronella oil, tea tree oil, and lemongrass oil to check its solubility. The calibration curve obtained for naringin using the developed method was found to be robust (standards relative standard deviation (%RSD) = 1.54–1.95% at 1 nm difference in wavelength); exhibited linearity (R² = 0.9974); had a precision of %RSD 1.02–2.89% (intraday) and 1.09–3.4% (interday); and an accuracy revealed by the % recovery range 98.43–107.62% at three levels, i.e., 80, 100, and 120%. These values are comparable to those obtained for HPLC, LC-MS, and LC. The solubility test was carried out using this developed method, and naringin proved to be the most soluble in methanol (10.470 ± 0.03 mg/mL) and in tea tree oil (4.332 ± 0.015 mg/mL) among the polar and nonpolar solvents. The method is applied for naringin solubility determination.