UV Method for Determination of Novel Combination of Antimicrobials by Spectral Resolution and in silico Pharmacokinetic Study

Abstract

Tetracycline HCl and tinidazole combination drugs are used for antimicrobial therapy. In order to quantify them in dosage forms by UV spectroscopy, resolving the spectral overlap is necessary to avoid interference. The present study introduced four simple, sustainable, mathematically processed UV spectrophotometric methods for the simultaneous determination of tetracycline HCl and tinidazole in binary mixtures and tablet dosage forms. The approaches used for resolving spectral overlap include first derivative, area under curve, dual wave length, and ratio difference methods. The methods demonstrated linearity in the range of 10–40 μg/mL for both drugs, while detection limits were in the range of 1.562–2.974 μg/mL for tetracycline HCl and tinidazole showed 0.077–1.65 μg/mL. ANOVA results showed no statistical difference among the methods. Sustainability assessment made by MoGAPI, RGB, and BAGI declared the developed method to be sustainable and eco-friendly. In silico drug–drug interaction study with respect to CYP450 enzymes showed no drug interactions. The developed method showed good agreement with validation parameters and was found to be efficient, economical, free of toxic solvents, and the complex sample preparation procedures indicated its sustainability.