Iranian Journal of Science and Technology, Transactions A: Science最新文献_第3页

The Effect of Surface Roughness and Deposition Time on the Biocompatibility of Electrodeposited Hydroxyapatite Coating on Grit-Blasted AZ31B Mg Alloy 表面粗糙度和沉积时间对砂喷AZ31B镁合金电沉积羟基磷灰石涂层生物相容性的影响

IF 1.7 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-19 DOI: 10.1007/s40995-024-01701-0

F. Hussain, M. U. Manzoor, M. Kamran, F. Riaz, M. Ammar, A. Mansoor, H. M. R. Tariq, M. H. Hassan, M. Ishtiaq

{"title":"The Effect of Surface Roughness and Deposition Time on the Biocompatibility of Electrodeposited Hydroxyapatite Coating on Grit-Blasted AZ31B Mg Alloy","authors":"F. Hussain, M. U. Manzoor, M. Kamran, F. Riaz, M. Ammar, A. Mansoor, H. M. R. Tariq, M. H. Hassan, M. Ishtiaq","doi":"10.1007/s40995-024-01701-0","DOIUrl":"https://doi.org/10.1007/s40995-024-01701-0","url":null,"abstract":"<p>Magnesium alloys have demonstrated tremendous potential as bio-implants due to their superior biomedical properties. This study examines the influence of surface roughness on the electrodeposition of Hydroxyapatite (HA) coatings on AZ31B magnesium alloy samples. We investigated samples grit-blasted with quartz and alumina at various pressures and compared them with un-blasted samples to ensure a comprehensive analysis across different deposition times. Higher blasting pressure resulted in increased surface roughness. Overall, samples blasted with alumina exhibited greater surface roughness and thicker coatings. X-ray diffraction (XRD)analysis confirmed the presence of HA coatings. Scanning electron microscope (SEM) analysis revealed that HA coating thickness increased with longer deposition times. Open circuit potential (OCP) measurements indicated that alumina-blasted samples showed nearly complete coverage with a 10-minute HA deposition at 700 kPa (approximately − 0.336 V), with increased compactness and more positive OCP values observed with longer deposition times. Complete insulated coverage was achieved at 40 min, with OCP values of -0.075 V at 700 kPa and − 0.049 V at 1000 kPa. Statistical analysis using ANOVA demonstrated that both blasting pressure and deposition time significantly impact coating thickness, with blasting pressure having a primary effect on OCP. Higher OCP values indicated a 95% increase in corrosion resistance at a blasting pressure of 1000 kPa and a 40-minute deposition time for alumina-blasted samples. The Ca/P ratio (∼ 1.67) and XRD patterns consistently confirmed the HA phase.</p>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"53 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142216103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Antiproliferative, Cytotoxic and Proapoptotic Properties of Virkon-S in Carp Epithelioma Papulosum Cells 探索 Virkon-S 在鲤鱼上皮乳头状瘤细胞中的抗增殖、细胞毒性和促凋亡特性

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-17 DOI: 10.1007/s40995-024-01692-y

Hüseyin Izgördü, Canan Vejselova Sezer, Nesil Ertorun, Hatice Mehtap Kutlu

{"title":"Exploring the Antiproliferative, Cytotoxic and Proapoptotic Properties of Virkon-S in Carp Epithelioma Papulosum Cells","authors":"Hüseyin Izgördü, Canan Vejselova Sezer, Nesil Ertorun, Hatice Mehtap Kutlu","doi":"10.1007/s40995-024-01692-y","DOIUrl":"10.1007/s40995-024-01692-y","url":null,"abstract":"<div><p><i>Cyprinus carpio</i> is one of most produced fish species farmed in Turkey. There are many factors comprising with the viral diseases that reduce productivity and cause significant economic losses in carp production. <i>Epithelioma papulosum cyprini</i> (EPC) is of the viral diseases with high infection and mortality rates. For prevention of parasitic diseases disinfectants have been used but due to the potential genotoxicity and cytotoxic effects alternative non-toxic agents are required. The biocompatible, and non-toxic disinfectant Virkon-S is recommended by FAO for this manner but its application on <i>Epithelioma papulosum cyprini</i>, but its usage on this disease has not yet been researched. The aim of this study was to investigate the cytotoxic, antiproliferative and proapoptotic effects of Virkon-S on EPC cells. Cytotoxicity, morphological and ultrastructural changes were investigated by MTT assay, confocal and transmission electron microscopy (TEM) techniques. Cell death mode was investigated by Annexin-V and Caspase 3/7 analysis. Cytotoxicity and apoptosis was detected on Virkon-S treated EPC cells. Also, TEM results revealed that Virkon-S notably changed the ultrastructure of EPC cells together with indications like swollen mitochondria, loss of cristae and membrane blebbings. The study results showed that Virkon-S has cytotoxic, antiproliferative and proapoptotic effects on EPC cells and might be used for prevention of EPC disease after deeper <i>in vivo</i> investigations. These findings collectively suggest the induction of cytotoxicity on the cellular ultrastructure. Furthermore, in alignment with the findings from confocal microscopy, it can be concluded that Virkon-S triggers apoptosis in EPC cells.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"48 5","pages":"1125 - 1133"},"PeriodicalIF":1.4,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142216104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing Regulation of ERG and its Target Proteins by ERG Isoform 8 评估 ERG 异构体 8 对 ERG 及其靶蛋白的调控作用

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-15 DOI: 10.1007/s40995-024-01683-z

Rizwan Ullah Khan, Sidra Mumtaz, Muhammad Imran Sohail, Naila Malkani

{"title":"Assessing Regulation of ERG and its Target Proteins by ERG Isoform 8","authors":"Rizwan Ullah Khan, Sidra Mumtaz, Muhammad Imran Sohail, Naila Malkani","doi":"10.1007/s40995-024-01683-z","DOIUrl":"10.1007/s40995-024-01683-z","url":null,"abstract":"<div><p>Ets-related gene (ERG) is a key protein in vascular homeostasis. It has multiple isoforms. These isoforms have predominant nuclear localization except ERG isoform 8. This isoform can modify the nuclear localization of other isoforms and can down-regulate other isoforms. However, the mechanism of this regulation and its impact on ERG target proteins is still unclear. This study is designed to analyze the effects of ERG8 on functional aspects of other isoforms. For this purpose, the expressions of ERG3 target genes; VE-cad, sox4, cxcr4 were analyzed after ERG8 and ERG3 co-transfection. Increasing ERG8 to ERG3 ratios were transfected in 293-HEK cells. qPCR analysis was performed on total RNA extracted from transfected cells to evaluate the changes in expression of target genes. Results showed that ERG3 alone significantly increased the expression of these target genes (p˂0.05). However, the increasing concentrations of ERG8 progressively decreased the expression of the target genes significantly (p˂0.05). For in silico prediction of the nature of ERG3-ERG8 interaction, their three-dimensional models were built using I-TASSER and interaction was predicted using HADDOCK 2.4. HADDOCK scores show that ERG8 via its pointed (PNT) domain can interact with Ets domain of ERG3 (HADDOCK score; -28.956 +/- 15.881) as well as PNT domain (HADDOCK score; -4.426 +/- 7.770). Our results indicate that ERG8 affect the transcription factor activity of ERG3 by hindering its binding with the DNA either through PNT or Ets domain</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"48 5","pages":"1081 - 1086"},"PeriodicalIF":1.4,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142216105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonlinear Maps Preserving the Mixed Type Product ((Mdiamond N circ W)) on (*)-Algebras 在 $$*$$ -代数上保存混合类型积 $$(Mdiamond Ncirc W)$$ 的非线性映射

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-15 DOI: 10.1007/s40995-024-01666-0

Mohammad Aslam Siddeeque, Raof Ahmad Bhat, Abbas Hussain Shikeh

引用次数: 0

Synthesis of Trifluoromethyl Benzimidazoles with Potential Anti-Microbial Activities 具有潜在抗微生物活性的三氟甲基苯并咪唑的合成

IF 1.7 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-14 DOI: 10.1007/s40995-024-01664-2

Samir M. El Rayes, Ibrahim A. I. Ali, Walid Fathalla, Mohamed A. Ghanem, Afaf H. El-Sagheer, Mohamed S. Nafie

引用次数: 0

Optimal Convergence of Thermoelastic Contact Problem Involving Nonlinear Hencky-Type Materials with Friction Conditions 带有摩擦条件的非线性亨茨基材料热弹性接触问题的最佳收敛性

IF 1.7 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-06 DOI: 10.1007/s40995-024-01677-x

H. EL Khalfi, Z. Faiz, O. Baiz, H. Benaissa

引用次数: 0

Numerical Solution of Stochastic Fractional Integro-Differential Equations: The Poly-sinc Collocation Approach 随机分式积分微分方程的数值解法：多正弦配位法

IF 1.7 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-08-02 DOI: 10.1007/s40995-024-01672-2

Faezeh Bahmani, Ali Eftekhari

引用次数: 0

A Novel Operational Matrix Method for Solving the Fractional Delay Integro-Differential Equations with a Weakly Singular Kernel 用弱奇异内核求解分式延迟积分微分方程的新型运算矩阵法

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-07-30 DOI: 10.1007/s40995-024-01682-0

S. Yaghoubi, H. Aminikhah, K. Sadri

引用次数: 0

Qualitative Study of a Novel Fractional-Order Epidemic Model with Nonmonotone Incidences, Level of Awareness, and Quarantine Class 具有非单调事件、认知水平和检疫等级的新型分数阶流行病模型的定性研究

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-07-29 DOI: 10.1007/s40995-024-01656-2

Abhishek Kumar, Vishesh Lonial

{"title":"Qualitative Study of a Novel Fractional-Order Epidemic Model with Nonmonotone Incidences, Level of Awareness, and Quarantine Class","authors":"Abhishek Kumar, Vishesh Lonial","doi":"10.1007/s40995-024-01656-2","DOIUrl":"10.1007/s40995-024-01656-2","url":null,"abstract":"<div><p>During an epidemic outbreak, slowing the infection among the population can be achieved through two major approaches: raising awareness about the disease and quarantining the infected population. Elevating awareness levels among the population is essential for slowing down the infection. In this study, our goal is to propose and mathematically analyze a novel Caputo derivative-based fractional-order Susceptible–Aware–Infected–Quarantined–Recovered–Susceptible compartmental model. The model is developed by incorporating the level of awareness among the population, explicit non-monotone incidence rates for new infection cases, and a saturated quarantine rate for the infected population. Additionally, this study aims to capture the complex dynamics of infectious diseases in the absence of any vaccine to control the disease spread. The qualitative study of the model reveals two equilibria: an infection-free equilibrium and an endemic equilibrium. We obtain that the infection-free equilibrium is locally asymptotically stable when the basic reproduction number is below one. Furthermore, we investigate the model for the possible occurrence of multiple positive equilibria and examine the local stability of the endemic equilibrium, showing that it is locally asymptotically stable under certain conditions when the basic reproduction number is above unity. Finally, we present numerical simulations to support our analytical findings.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"48 5","pages":"1187 - 1209"},"PeriodicalIF":1.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corn Steep Liquor as an Auto-Induction Medium for the Production of rhIFN-β-1b Protein in Escherichia coli 玉米淀粉液作为大肠杆菌生产 rhIFN-β-1b 蛋白的自动诱导培养基

IF 1.4 4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2024-07-29 DOI: 10.1007/s40995-024-01684-y

Nasira Rafi, Rabia Abbas, Nadeem Ahmed, Saad Tahir, Muhammad Akram, Mohsin Ahmad Khan, Nao Akusa Fujimura, Mariam Shafique, Kausar Malik

{"title":"Corn Steep Liquor as an Auto-Induction Medium for the Production of rhIFN-β-1b Protein in Escherichia coli","authors":"Nasira Rafi, Rabia Abbas, Nadeem Ahmed, Saad Tahir, Muhammad Akram, Mohsin Ahmad Khan, Nao Akusa Fujimura, Mariam Shafique, Kausar Malik","doi":"10.1007/s40995-024-01684-y","DOIUrl":"10.1007/s40995-024-01684-y","url":null,"abstract":"<div><p>Therapeutic proteins need to be produced in large quantities as they continuously gain higher shares in pharmaceutical market. Human beta interferon (hIFN-β) is used against different diseases due to its antiviral, antiproliferative and immunomodulatory nature. Considering its clinical applications, development of a simple, cost-effective and easily scalable production system to produce rhIFN-β-1b protein in high yield and quality is needed. Attempts to develop improved production methods are ongoing. Majority of previous reports propose isopropyl-β-D-1-thiogalactopyranoside (IPTG), for rhIFN-β-1b expression in <i>Escherichia coli</i>, which is costly and nonmetabolizable. Thus, in current study, rhIFN-β-1b expression was optimized in an inexpensive auto-induction medium. Corn steep liquor (CSL) was used and optimized at different concentrations. Further, the medium was also optimized for carbon sources using various glycerol concentrations. Finally, a 10 L fermentation batch was done (10% CSL and 2.5% glycerol) that produced 65 g of wet biomass and 15 g of inclusion body (wet). The purification scheme was optimized by one-step ion-exchange chromatography (IEX) for > 99% purity of the target protein. The biological activity of the required protein was assessed through cytopathic inhibition effect of virus vesicular stomatitis (VSV) on Madin-Darby bovine kidney (MDBK) cells. Moreover, cytotoxicity of the protein was also studied on different breast cancer cell lines; HCC- 1954, MCF- 7 and MDA- MB- 231. This is the first report on utilizing CSL as auto-induction media for expression and purification of rhIFN-β-1b. Thus, the suggested method can be employed for simple as well as cost-effective production of rhIFN-β-1b and other recombinant proteins.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"48 5","pages":"1087 - 1098"},"PeriodicalIF":1.4,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141870670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0