Iranian Journal of Science and Technology, Transactions A: Science最新文献

Nickel (II) Phenylthiosemicarbazone Complex Induces Cytotoxicity, Oxidative Stress and Apoptosis in Human Leukemia Stem-like KG1a Cells 镍(II)苯基硫代氨基脲复合物诱导人白血病干细胞样KG1a细胞的细胞毒性、氧化应激和凋亡

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-17 DOI: 10.1007/s40995-023-01509-4

Shahrebanoo Gholizadeh, Farhad Eslami, Sanam Arami, Gholamreza Dehghan, Seyed Abolfazl Hosseini-Yazdi, Saeed Hesami Tackallou, Majid Mahdavi

{"title":"Nickel (II) Phenylthiosemicarbazone Complex Induces Cytotoxicity, Oxidative Stress and Apoptosis in Human Leukemia Stem-like KG1a Cells","authors":"Shahrebanoo Gholizadeh, Farhad Eslami, Sanam Arami, Gholamreza Dehghan, Seyed Abolfazl Hosseini-Yazdi, Saeed Hesami Tackallou, Majid Mahdavi","doi":"10.1007/s40995-023-01509-4","DOIUrl":"10.1007/s40995-023-01509-4","url":null,"abstract":"<div><p>Thiosemicarbazones (TSCs) are compounds with different biological properties. Previous studies suggested that Thiosemicarbazones and their metal complexes have potential cytotoxic effects against numerous cancers. In the present study, the anti-proliferative effect and oxidative stress-induced apoptosis in the human leukemia stem-like KG1a cells by Nickel (II) phenylthiosemicarbazone complex (4-NPTC) were evaluated. For this purpose, the KG1a cells were treated with different concentrations of the 4-NPTC to determine the IC<sub>50</sub> value by MTT assay. We investigated the impact of 4-NPTC on induction of oxidative stress by measuring the activity of two antioxidant enzymes, catalase (CAT) and superoxide dismutase (SOD), and by assessing the level of biomarkers such as malondialdehyde (MDA), total thiol and ROS levels in the leukemia stem-like KG1a cells. Moreover, we evaluated the influence of 4-NPTC on the cell cycle progression using flow cytometry. Results showed that 4-NPTC enhanced the activity of antioxidant enzymes in the cells after 24 h exposure and decreased their activity when the exposure period to 4-NPTC was prolonged to 48 h and 72 h. Furthermore, the notably enhance of intracellular ROS levels after 48 and 72 h was observed. In addition, the diminution of intracellular thiol enhanced in MDA amounts was observed in treated KG1a cells. The G0/G1 phase cell cycle arrest followed by induction of apoptosis was shown in KG1a cells after treatment with the 4-NPTC. Together, the results demonstrate that 4-NPTC plays important role in blocking of development of the leukemic cells by inducing oxidative stress and apoptosis.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"47 4","pages":"1125 - 1135"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4671224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

n-th Derivative Hardy Spaces and Weighted Differentiation Composition Operators n阶导数Hardy空间与加权微分复合算子

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-17 DOI: 10.1007/s40995-023-01503-w

Mostafa Hassanlou, Ebrahim Abbasi, Sepideh Nasresfahani

引用次数: 1

Assessing the Strength and Deformation Properties of Serpentinite Rocks in Dry and Saturated Conditions 蛇纹岩在干燥和饱和条件下的强度和变形特性评估

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-17 DOI: 10.1007/s40995-023-01512-9

Konstantinos Diamantis, Davood Fereidooni

{"title":"Assessing the Strength and Deformation Properties of Serpentinite Rocks in Dry and Saturated Conditions","authors":"Konstantinos Diamantis, Davood Fereidooni","doi":"10.1007/s40995-023-01512-9","DOIUrl":"10.1007/s40995-023-01512-9","url":null,"abstract":"<div><p>It is clear that the engineering properties of various rocks in dry and wet conditions are different. In general, the properties decrease with the presence of water. The present research investigates the effect of rock saturation on the strength and deformation properties of serpentinites collected from central Greece. A comprehensive laboratory test program was planned to evaluate unit weight, effective porosity, uniaxial compressive strength, elasticity modulus, and Brazilian tensile strength in saturated states. All the results of the above-mentioned saturated properties were compared with the respective properties measured in dry conditions taken from previous studies. Simple regression analyses were used to correlate the physical and mechanical properties of the two referred states. The obtained results revealed that the values of the considered physical and mechanical properties of the studied serpentinite rocks are deceased with the presence of water. There are good relations between different rock properties in dry and saturated states. Generally, the relations are better in dry state. All correlations between the same rock properties in dry and saturated states are as direct linear relations, whereas relationships between the mechanical and physical properties are mainly exponential. Some of them are logarithmic or power equations.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"47 4","pages":"1169 - 1178"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s40995-023-01512-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4670460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

q-Gamma Type Operators for Approximating Functions of a Polynomial Growth 逼近多项式生长函数的q-Gamma型算子

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-16 DOI: 10.1007/s40995-023-01507-6

Purshottam Narain Agrawal, Behar Baxhaku, Ruchi Chauhan

引用次数: 0

Generalized Operator Shannon Entropy and Related Operator Inequalities 广义算子香农熵与相关算子不等式

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-16 DOI: 10.1007/s40995-023-01510-x

Ismail Nikoufar, Kenjiro Yanagi

引用次数: 0

An Accelerated Iterative Method to Find the Sign of a Nonsingular Matrix with Quartical Convergence 求四次收敛非奇异矩阵符号的加速迭代法

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-09 DOI: 10.1007/s40995-023-01506-7

Yan Feng, Ahmed Zaka Othman

引用次数: 0

Conditioned Medium Reduces Isoproterenol-Induced Myocardial Damage Via MAPK/NF-κB Pathway 条件培养基通过MAPK/NF-κB途径减少异丙肾上腺素诱导的心肌损伤

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-08-07 DOI: 10.1007/s40995-023-01504-9

Maryam Naseroleslami, Nahid Aboutaleb

{"title":"Conditioned Medium Reduces Isoproterenol-Induced Myocardial Damage Via MAPK/NF-κB Pathway","authors":"Maryam Naseroleslami, Nahid Aboutaleb","doi":"10.1007/s40995-023-01504-9","DOIUrl":"10.1007/s40995-023-01504-9","url":null,"abstract":"<div><p>The present work was conducted to examine the cardioprotective effects of conditioned medium (CM) obtained from human amniotic membrane mesenchymal stem cells (hAMSCs-CM) in a rat model of isoproterenol (ISO)-induced myocardial damage and to clarify its effect on activity of mitogen-activated protein kinase (MAPK)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway. ISO was subcutaneously injected at 170 mg/kg/day for 4 consecutive days to create model. The cardiac function was determined using echocardiography. The expression of inflammatory cytokine was investigated using enzyme-linked immunosorbent assay (ELISA). The activity of MAPK/NF-κB was evaluated by immunohistochemistry assay. Trichrome Masson’s staining was used to evaluate the fibrosis. ISO-induced myocardial damage was indicated by loss of cardiac function, excessive secretion of inflammatory cytokines, increased fibrosis and activity of NF-κB and increasing phosphorylated levels of p38 MAPK. Intramyocardial post-treatment with 150 µL of hAMSCs-CM significantly improved heart function and reduced fibrosis and expression levels of inflammatory cytokines, NF-κB expression, and phosphorylated levels of p38 MAPK (<i>p</i> < 0. 05). Collectively, findings of present study demonstrate that cardioprotective effects of hAMSCs-CM are associated with attenuation of inflammation through reduction in activity of MAPK/NF-κB pathway. This can be attributed to paracrine factors secreted by MSCs including exosomes, cytokines, antiapoptotic, angiogenic, and growth factors.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"47 4","pages":"1115 - 1124"},"PeriodicalIF":0.0,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4284514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Study on the Binding of Anti-cancer Drug (Floxuridine) with Human Serum Albumin 抗癌药物氟尿定与人血清白蛋白结合的综合研究

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-07-31 DOI: 10.1007/s40995-023-01502-x

Somaye Shahraki, Hojat Samareh Delarami, Mahdiye Poorsargol, Zohreh Razmara, Mostafa Heidari Majd

{"title":"A Comprehensive Study on the Binding of Anti-cancer Drug (Floxuridine) with Human Serum Albumin","authors":"Somaye Shahraki, Hojat Samareh Delarami, Mahdiye Poorsargol, Zohreh Razmara, Mostafa Heidari Majd","doi":"10.1007/s40995-023-01502-x","DOIUrl":"10.1007/s40995-023-01502-x","url":null,"abstract":"<div><p>Floxuridine, FUDR, is used to treat colorectal and other cancers such as kidney and stomach cancers. Spectroscopic techniques along with molecular docking and molecular dynamic analyzes were used to evaluate the association of FUDR with human serum albumin (HSA). Our results showed that FUDR interacts with HSA via a static, exothermic, enthalpy-driven mechanism. The binding constants (K<sub>b</sub>) of FUDR-HSA were 7.58 ± 0.63, 3.89 ± 0.32 and 2.00 ± 0.11 × 10<sup>4</sup> M<sup>−1</sup> at 303, 310 and 317 K, respectively. The most important forces that were most influential in the drug-protein interaction process were van der Waals forces and hydrogen bonds. Compared with the tyrosine (Tyr), the tryptophan (Trp) residue showed a higher quenching rate suggested that Trp residue was closer to the interaction site of FUDR. The CD spectra have shown that HSA secondary structure changes to a limited extent during the FUDR interaction process. The docking analysis showed the locations of the binding sites for FUDR on HSA (locating in site I). However, despite the binding of the FUDR to albumin, changes in protein structure are limited, and it can be hoped that after delivery to the target organ, the HSA can resume its biological activity.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"47 4","pages":"1155 - 1167"},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5177303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On Metric Pressure of Random Dynamical Systems: A Local Viewpoint 随机动力系统的公制压力:一个局部观点

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-07-29 DOI: 10.1007/s40995-023-01500-z

M. Rahimi, A. Ghodrati

引用次数: 0

Swift and Precise Determination of Rifampicin by RP-HPLC Technology Development in Pharmaceuticals 反相高效液相色谱法快速精确测定利福平

4区综合性期刊

Iranian Journal of Science and Technology, Transactions A: Science Pub Date : 2023-07-28 DOI: 10.1007/s40995-023-01471-1

Mohd. Aqil, Mamta Bishnoi, Meenakshi Kanwar Chauhan

{"title":"Swift and Precise Determination of Rifampicin by RP-HPLC Technology Development in Pharmaceuticals","authors":"Mohd. Aqil, Mamta Bishnoi, Meenakshi Kanwar Chauhan","doi":"10.1007/s40995-023-01471-1","DOIUrl":"10.1007/s40995-023-01471-1","url":null,"abstract":"<div><p>Rifampicin (RIF) is a first-line antituberculous agent that binds with polymerase subunit and inhibits bacterial DNA-dependent RNA polymerase, permitting direct blocking of the elongating RNA. The study aimed to develop a better, more precise, and easy reversed-phase HPLC process that used readily available, less expensive chemicals and materials in an isocratic mode. The method guarantees that accuracy, precision, robustness, and other requirements are fulfilled and satisfied as specified by the ICH or FDA rules. According to the ICH recommendations, analytical characteristics including linearity, accuracy, specificity precision, detection limit, and quantification limit were determined. Rifampicin was separated chromatographically using the mobile phase combination, acetonitrile, and methanol (80:20 v/v) with a C-8 column at the speed of 1.7 mL/min in both pumps with a continuous run time of up to 2 min. The recovery of RIF extended from 98.9 to 100.8 with a relative standard deviation (% RSD) of about 1.5%. A stability investigation study showed that the drugs were stable for a long enough period of time. The limit of measurement for RIF was found to be 10 µg/mL. The average recovery rate was obtained at 98.96%. The study supported the retention times of the analytes, and the chromatographic runs were accurate and free of interfering peaks. This method of analysis is useful in pharmacokinetics and bioequivalence studies.</p></div>","PeriodicalId":600,"journal":{"name":"Iranian Journal of Science and Technology, Transactions A: Science","volume":"47 4","pages":"1137 - 1144"},"PeriodicalIF":0.0,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s40995-023-01471-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5081302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0