园艺研究(英文)最新文献

{"title":"The transcription factor AebHLH89 activates <i>AeGMP1</i> transcription to regulate L-ascorbic acid accumulation in kiwifruit (<i>Actinidia eriantha</i>) revealed by genome-wide association study.","authors":"Lu Chen, Dongfeng Jia, Yansong Liu, Huan Gao, Guanglian Liao, Jipeng Mao, Zhu Gao, Xiaobiao Xu","doi":"10.1093/hr/uhag116","DOIUrl":"10.1093/hr/uhag116","url":null,"abstract":"<p><p><i>Actinidia eriantha</i>, a kiwifruit species endemic to China, produces fruits with notable nutritional, medicinal, and economic value, particularly due to its high L-ascorbic acid (L-AsA) content. However, the regulatory mechanisms underlying L-AsA accumulation in its fruit remain poorly understood. This study meticulously measured L-AsA levels of fruits in 216 <i>A. eriantha</i> accessions from natural populations and performed a genome-wide association study, through which we identified significantly associated lead single nucleotide polymorphisms and insertion deletions, and characterized a key candidate gene <i>AebHLH89</i>, <i>AePPR</i>, <i>AePP2Ab</i>, and <i>AePHL1</i> involved in the positive regulation of L-AsA accumulation. Functional experiments showed that overexpression of <i>AebHLH89</i> significantly enhanced L-AsA accumulation, while its silencing <i>via</i> virus-induced gene silencing markedly decreased L-AsA levels. Yeast one-hybrid assay and dual-luciferase assay preliminary revealed that AebHLH89 could bind to the <i>AeGMP1</i> promoter and activates its transcription, thereby upregulating the L-AsA biosynthesis pathway and promoting L-AsA synthesis and accumulation. These findings provide valuable genetic resources for molecular marker-assisted breeding in kiwifruit and contribute to germplasm innovation. Simultaneously, the identification of key regulatory genes enhances our understanding of L-AsA metabolism and lays a theoretical foundation for the genetic improvement of kiwifruit.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag116"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13341127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148407036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome and machine learning analyses reveal 500-bp promoter chromatin accessibility as a key regulator of gene expression and catechin biosynthesis in <i>Camellia sinensis</i>.","authors":"Yuan Gao, Wenmin Fan, Wenlong Lei, Yibing Chen, Xueli Liao, Yezi Xiao, Lanying Li, Xiaomei Lei, Wanjun Gao, Yingao Zhang, Hongyan Deng, Jiawei Yan, Zongjie Wu, Chen Liang, Xinru Hou, Youben Yu, Fan Luo, Pengjie Wang, Dongna Liu","doi":"10.1093/hr/uhag129","DOIUrl":"10.1093/hr/uhag129","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag129"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13417467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel pyrimidine-guanidine derivative YYH-6 confers tomato resistance to TYLCV via multitarget regulation of host defense genes and proteins.","authors":"Miao Yu, Kexin Liu, Jing Shu, Jing Yang, Yuhang Yang, Dongyang Liu, Ting Liu, Lianqiang Jiang, Zhiping Wang, Zihao Xia, Mengnan An, Xinghai Li, Yuanhua Wu","doi":"10.1093/hr/uhag120","DOIUrl":"10.1093/hr/uhag120","url":null,"abstract":"<p><p>Tomato yellow leaf curl virus (TYLCV) poses a serious threat to global tomato production. Current management strategies remain limited, highlighting the need for novel antiviral agents. In this study, we evaluated the efficacy of a newly synthesized pyrimidine-guanidine derivative, YYH-6, against TYLCV in tomato (<i>Solanum lycopersicum</i> cv. Micro-Tom) and investigated its underlying mechanisms. Foliar application of YYH-6 significantly reduced viral accumulation and alleviated disease symptoms. Transcriptomic analysis revealed that YYH-6 treatment upregulates defense-related pathways such as plant-pathogen interaction, MAPK signaling, and phenylpropanoid biosynthesis. Results of TRV-based virus-induced gene silencing (VIGS) further indicate that <i>RPP13</i> and <i>ALS2</i> function as important resistance genes induced by YYH-6. Activity-based protein profiling, LC-MS/MS, and reverse genetic verification by VIGS indicate that serine hydroxymethyltransferase and STI1 domain-containing proteins are potential target proteins of YYH-6 for TYLCV inhibition. Our findings demonstrate that YYH-6 suppresses TYLCV infection through multitarget modulation of host defense pathways, making it a promising candidate for sustainable management of tomato viral diseases.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 8","pages":"uhag120"},"PeriodicalIF":9.5,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13395516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148581408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cis</i>-regulatory effects of transposable element insertion/absence polymorphisms in the <i>Brassica napus</i> population.","authors":"Guoxiang Yuan, Yang Huang, Chunlu Tao, Minxin Li, Qinxia Dai, Yirong Wang, Huaixin Li, Xiangdong Kong, Zongji Zhang, Ri Ming, Li Zhong, Yu Liang, Maoteng Li","doi":"10.1093/hr/uhaf356","DOIUrl":"10.1093/hr/uhaf356","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 4","pages":"uhaf356"},"PeriodicalIF":9.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13365621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148450736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis reveals the molecular mechanism underlying poplar 107 rootstock-mediated regulation of <i>Populus tomentosa</i> scion growth.","authors":"Jianghao Wu, Yachao Ren, Chunyu Wang, Kaiyu Yang, Min Jiang, Jinmao Wang, Minsheng Yang","doi":"10.1093/hr/uhag086","DOIUrl":"10.1093/hr/uhag086","url":null,"abstract":"<p><p>Grafting is widely used for asexual propagation and enhancing plant productivity; however, the molecular mechanisms underlying rootstock-scion interactions remain largely unclear. In this study, <i>Populus</i> <i>×</i> <i>euramericana</i> cv. 'Neva' (poplar 107) served as rootstock and <i>Populus tomentosa</i> 'Yixian' (<i>P. tomentosa</i>) as scion for grafting. By integrating physiological measurements, transcriptome sequencing [messenger RNA (mRNA) and long non-coding RNA (lncRNA)], widely targeted metabolomics, and mobile RNA identification revealed that heterografted scions had increased metabolites related to carbon fixation and metabolism, decreased metabolites associated with respiration and defense, and enhanced net photosynthetic rate, potentially promoting height growth; differential expression lncRNAs may represent an important molecular basis for accelerated scion growth; in scions, mobile mRNA-associated differential mRNAs primarily involved amino acid biosynthesis, while mobile lncRNA-associated ones focused on energy metabolism; no target mRNAs of mobile lncRNAs were found in the rootstock; both mRNAs and lncRNAs were transported in full-length and fragmented forms, and downward RNA transport showed a significant correlation with transcript abundance; in recipient tissues, mobile mRNAs were less abundant while mobile lncRNAs were more abundant than in donor tissues; the flavonoid biosynthesis pathway associated with mobile RNAs played an important role in rootstock-mediated scion growth, and kaempferol, as a key metabolite, reduced reactive oxygen species-induced damage, increased net photosynthetic rate, and promoted growth in <i>Nicotiana benthamiana</i>. This study provides an initial insight into the transport and regulatory patterns of mRNAs and lncRNAs in woody grafted plants, offering a theoretical basis for the rational application of grafting technology.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag086"},"PeriodicalIF":9.5,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13291916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148334904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9-mediated disruption of <i>MdGA20ox</i> confers dwarfism in apple.","authors":"Nan Guo, Ningwang Huang, Yao Xiong, Doudou Chen, Haochen Sun, Qian Yang, Haorui Zhang, Yi Wang, Zhenhai Han, Wei Li","doi":"10.1093/hr/uhag083","DOIUrl":"10.1093/hr/uhag083","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag083"},"PeriodicalIF":9.5,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13267397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148267117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of <i>CsYP</i> in regulating chloroplast development and cucumber peel color.","authors":"Yuanyuan Cui, Sen Li, Haoying Wu, Xi Zhao, Xiao Zhang, Yuming Dong, Yaru Wang, Menghang An, Lin Yang, Xiaofeng Chen, Yiqun Weng, Huazhong Ren, Xingwang Liu","doi":"10.1093/hr/uhag043","DOIUrl":"10.1093/hr/uhag043","url":null,"abstract":"<p><p>Skin color is a crucial quality trait in cucumber fruit, yet the regulatory mechanisms underlying cucumber skin color remain poorly understood. In this study, we characterized a cucumber natural mutant displaying yellow peel, and identified a key gene <i>yellow peel</i> (<i>CsYP</i>) through map-based cloning. <i>CsYP</i> encodes a rhodanese-like protein with a Rhod domain. A single-base insertion results in premature termination of protein translation, leading to the yellowing pericarp phenotype in the natural mutant. To further investigate the function of CsYP, two knockout lines, <i>yp-1</i> and <i>yp-2</i>, were generated using CRISPR-Cas9 technology. Phenotypic investigation of <i>yp-1</i> and <i>yp-2</i> revealed a significant yellowing of the pericarp starting from 6 days after pollination, consistent with the natural mutant phenotype. Additionally, our study revealed an interaction between CsYP and Cscytb6f, a cytochrome b6-f complex iron-sulfur subunit, suggesting a collaborative role of CsYP and iron-sulfur proteins in regulating cucumber peel color. These findings provide novel insights into the regulatory mechanisms underlying cucumber peel color and broaden our understanding of this important trait.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 5","pages":"uhag043"},"PeriodicalIF":9.5,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147870620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive genome evolution and metabolic rewiring in the highland poppy <i>Meconopsis integrifolia</i>.","authors":"Lamu YangJin, Yi Yuan, Siheng Zeng, Guojin Hu, Mingyang Du, Huiyan Xiong, Xianqing Jia, Ruijun Duan","doi":"10.1093/hr/uhag045","DOIUrl":"10.1093/hr/uhag045","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 5","pages":"uhag045"},"PeriodicalIF":9.5,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13222481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148145641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Petal senescence in cut roses.","authors":"Saretta N Paramita, Denis Saint-Marcoux, Sylvie Baudino, Guillaume Beaugey, Sonja Meilland, Jean-Claude Caissard","doi":"10.1093/hr/uhaf342","DOIUrl":"10.1093/hr/uhaf342","url":null,"abstract":"<p><p>Roses (<i>Rosa</i> sp.) are highly valued ornamental plants, with over 25 000 cultivars created by breeders, among which cut roses dominate the global flower market. Flowers of these cultivars can last up to 20 days in a vase from the moment they are cut, which is not the case for garden roses. This review examines whether the vase life of cut roses resembles or differs from natural flower senescence, focusing on the phytohormonal processes involved in both scenarios. We first compare petal senescence with other senescence phenomena and then examine genes related to hormone action. Finally, we show the similarities between senescence in cut roses and that of standing roses. We conclude that, despite the existence of similarities, including the involvement of ethylene in petal senescence, comparative studies between cut and uncut roses would be useful, both for basic research and to improve the selection of varieties with long vase life.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 3","pages":"uhaf342"},"PeriodicalIF":9.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999221/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147488654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The transcription factor <i>PnMYB38</i> orchestrates methyl jasmonate-induced saponin biosynthesis in <i>Panax notoginseng</i>.","authors":"Zhi Yang, Lifang Yang, Zhiyao Zhu, Min Li, Jiae Hou, Shuying Wang, Huanzhen Wu, Qian Yang, Xiuming Cui, Yonghong Tao, Ye Yang, Yuan Liu","doi":"10.1093/hr/uhag052","DOIUrl":"10.1093/hr/uhag052","url":null,"abstract":"<p><p><i>Panax notoginseng</i> is an important medicinal plant, and saponins are the primary active components that are the key determinants of pharmaceutical quality. Research has indicated that methyl jasmonate (MeJA) enhances the saponins accumulation in <i>P. notoginseng</i>, but the specific MeJA-responsive transcription factors (TFs) that regulate this process remains unidentified. Given the critical role of <i>MYB</i> TFs in the regulation of plant secondary metabolism, this study aimed to elucidate the regulatory mechanisms of the <i>MYB</i> TF family in <i>P. notoginseng</i> under MeJA treatment. Genome-wide screening led to the identification 110 <i>MYB</i> genes, followed by a comprehensive analysis of their phylogenetic relationships, conserved motifs, gene structures, cis-acting elements, chromosomal localization, and collinearity. Integrated transcriptomic and metabolomic analyses showed that MeJA treatment significantly altered the expression patterns of 84 <i>MYB</i> genes while also promoting saponin accumulation in <i>P. notoginseng</i> leaves. Co-expression network analysis revealed a significant correlation between <i>PnMYB38</i> and saponin metabolites, highlighting the pivotal regulatory function of this TF. Subcellular localization experiments confirmed nuclear localization of <i>PnMYB38</i>. Yeast one-hybrid, electrophoretic mobility shift assay, and dual-luciferase assays demonstrated that <i>PnMYB38</i> directly and specifically bound to the promoters of key saponin biosynthesis genes (<i>PnSE</i> and <i>PnDS</i>), thereby inducing their expression. This study comprehensively characterized the functional role of <i>PnMYB38</i> in regulating MeJA-mediated saponin biosynthesis in <i>P. notoginseng</i>, proposed a 'MeJA-<i>PnMYB38</i>-saponin biosynthesis' regulatory network that provided novel insights into the transcriptional regulatory mechanism of saponin biosynthesis, and established a foundation for molecular breeding targeting <i>MYB</i> TFs and metabolic engineering.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 6","pages":"uhag052"},"PeriodicalIF":9.5,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13241188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148201373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}