园艺研究(英文)最新文献

{"title":"Identification and characterization of two P450 enzymes from <i>Citrus sinensis</i> involved in TMTT and DMNT biosyntheses and Asian citrus psyllid defense.","authors":"Xueli Sun, Chunhua Hu, Ganjun Yi, Xinxin Zhang","doi":"10.1093/hr/uhae037","DOIUrl":"https://doi.org/10.1093/hr/uhae037","url":null,"abstract":"<p><p>The homoterpenes (3<i>E</i>)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (<i>E</i>,<i>E</i>)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are the major herbivore-induced plant volatiles that help in defense directly by acting as repellants and indirectly by recruiting insects' natural enemies. In this study, DMNT and TMTT were confirmed to be emitted from citrus (<i>Citrus sinensis</i>) leaves infested with Asian citrus psyllid (<i>Diaphorina citri</i> Kuwayama; ACP), and two cytochrome P450 (CYP) genes (<i>CsCYP82L1</i> and <i>CsCYP82L2</i>) were newly identified and characterized. Understanding the functions of these genes in citrus defense will help plan strategies to manage huanglongbing caused by <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) and spread by ACP. Quantitative real-time PCR (qPCR) analysis showed that <i>CsCYP82L1</i> and <i>CsCYP82L2</i> were significantly upregulated in citrus leaves after ACP infestation. Yeast recombinant expression and enzyme assays indicated that CsCYP82L1 and CsCYP82L2 convert (<i>E</i>)-nerolidol to DMNT and (<i>E,E</i>)-geranyllinalool to TMTT. However, citrus calluses stably overexpressing <i>CsCYP82L1</i> generated only DMNT, whereas those overexpressing <i>CsCYP82L2</i> produced DMNT and TMTT. Furthermore, ACPs preferred wild-type lemon (<i>Citrus limon</i>) over the <i>CsCYP82L1</i>-overexpressing line in dual-choice feeding assays and mineral oil over TMTT or DMNT in behavioral bioassays. Finally, yeast one-hybrid, electrophoretic mobility shift, and dual luciferase assays demonstrated that CsERF017, an AP2/ERF transcription factor, directly bound to the CCGAC motif and activated <i>CsCYP82L1</i>. Moreover, the transient overexpression of <i>CsERF017</i> in lemon leaves upregulated <i>CsCYP82L1</i> in the absence and presence of ACP infestation. These results provide novel insights into homoterpene biosynthesis in <i>C. sinensis</i> and demonstrate the effect of homoterpenes on ACP behavior, laying a foundation to genetically manipulate homoterpene biosynthesis for application in huanglongbing and ACP control.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 4","pages":"uhae037"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11009467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of <i>O</i>-methyltransferases in the biosynthesis of phenylphenalenone phytoalexins based on the telomere-to-telomere gapless genome of <i>Musella lasiocarpa</i>.","authors":"Wanli Zhao, Junzhi Wu, Mei Tian, Shu Xu, Shuaiya Hu, Zhiyan Wei, Guyin Lin, Liang Tang, Ruiyang Wang, Boya Feng, Bi Wang, Hui Lyu, Christian Paetz, Xu Feng, Jia-Yu Xue, Pirui Li, Yu Chen","doi":"10.1093/hr/uhae042","DOIUrl":"10.1093/hr/uhae042","url":null,"abstract":"<p><p>Phenylphenalenones (PhPNs), phytoalexins in wild bananas (Musaceae), are known to act against various pathogens. However, the abundance of PhPNs in many Musaceae plants of economic importance is low. Knowledge of the biosynthesis of PhPNs and the application of biosynthetic approaches to improve their yield is vital for fighting banana diseases. However, the processes of PhPN biosynthesis, especially those involved in methylation modification, remain unclear. <i>Musella lasiocarpa</i> is a herbaceous plant belonging to Musaceae, and due to the abundant PhPNs, their biosynthesis in <i>M. lasiocarpa</i> has been the subject of much attention. In this study, we assembled a telomere-to-telomere gapless genome of <i>M. lasiocarpa</i> as the reference, and further integrated transcriptomic and metabolomic data to mine the candidate genes involved in PhPN biosynthesis. To elucidate the diversity of PhPNs in <i>M. lasiocarpa</i>, three screened <i>O</i>-methyltransferases (Ml01G0494, Ml04G2958, and Ml08G0855) by phylogenetic and expressional clues were subjected to <i>in vitro</i> enzymatic assays. The results show that the three were all novel <i>O</i>-methyltransferases involved in the biosynthesis of PhPN phytoalexins, among which Ml08G0855 was proved to function as a multifunctional enzyme targeting multiple hydroxyl groups in PhPN structure. Moreover, we tested the antifungal activity of PhPNs against <i>Fusarium oxysporum</i> and found that the methylated modification of PhPNs enhanced their antifungal activity. These findings provide valuable genetic resources in banana breeding and lay a foundation for improving disease resistance through molecular breeding.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 4","pages":"uhae042"},"PeriodicalIF":7.6,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11528125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation of mesophyll conductance mediated by nitrogen form is related to changes in cell wall property and chloroplast number.","authors":"Yiwen Cao, Yonghui Pan, Yating Yang, Tianheng Liu, Min Wang, Yong Li, Shiwei Guo","doi":"10.1093/hr/uhae112","DOIUrl":"10.1093/hr/uhae112","url":null,"abstract":"<p><p>Plants primarily incorporate nitrate (NO₃ ^-) and ammonium (NH₄ ⁺) as the primary source of inorganic nitrogen (N); the physiological mechanisms of photosynthesis (<i>A</i>) dropdown under NH₄ ⁺ nutrition has been investigated in many studies. Leaf anatomy is a major determinant to mesophyll conductance (<i>g</i> _m) and photosynthesis; however, it remains unclear whether the photosynthesis variations of plants exposed to different N forms is related to leaf anatomical variation. In this work, a common shrub, <i>Lonicera japonica</i> was hydroponically grown under NH₄ ⁺, NO₃ ^- and 50% NH₄ ⁺/NO₃ ^-. We found that leaf N significantly accumulated under NH₄ ⁺, whereas the photosynthesis was significantly decreased, which was mainly caused by a reduced <i>g</i> _m. The reduced <i>g</i> _m under NH₄ ⁺ was related to the decreased intercellular air space, the reduced chloroplast number and especially the thicker cell walls. Among the cell wall components, lignin and hemicellulose contents under NH₄ ⁺ nutrition were significantly higher than those in the other two N forms and were scaled negatively correlated with <i>g</i> _m; while pectin content was independent from N forms. Pathway analysis further revealed that the cell wall components might indirectly regulate <i>g</i> _m by influencing the thickness of the cell wall. These results highlight the importance of leaf anatomical variation characterized by modifications of chloroplasts number and cell wall thickness and compositions, in the regulation of photosynthesis in response to varied N sources.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 6","pages":"uhae112"},"PeriodicalIF":7.6,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11197310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered dsRNA-protein nanoparticles for effective systemic gene silencing in plants.","authors":"Huayu Sun, Ankarao Kalluri, Dan Tang, Jingwen Ding, Longmei Zhai, Xianbin Gu, Yanjun Li, Huseyin Yer, Xiaohan Yang, Gerald A Tuskan, Zhanao Deng, Frederick G Gmitter, Hui Duan, Challa Kumar, Yi Li","doi":"10.1093/hr/uhae045","DOIUrl":"https://doi.org/10.1093/hr/uhae045","url":null,"abstract":"<p><p>Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA) to form nucleic acid-protein nanocomplexes. In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots. We have further demonstrated that the cBSA/dsGUS RNA nanocomplexes are highly effective in silencing both the conditionally inducible <i>DR5-GUS</i> gene and the constitutively active <i>35S-GUS</i> gene in leaf, shoot, and shoot meristem tissues. This cBSA/dsRNA delivery technology may provide a convenient, fast, and inexpensive tool for characterizing gene functions in plants and potentially for <i>in planta</i> gene editing.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 4","pages":"uhae045"},"PeriodicalIF":7.6,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11497610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant-LncPipe: a computational pipeline providing significant improvement in plant lncRNA identification.","authors":"Xue-Chan Tian, Zhao-Yang Chen, Shuai Nie, Tian-Le Shi, Xue-Mei Yan, Yu-Tao Bao, Zhi-Chao Li, Hai-Yao Ma, Kai-Hua Jia, Wei Zhao, Jian-Feng Mao","doi":"10.1093/hr/uhae041","DOIUrl":"https://doi.org/10.1093/hr/uhae041","url":null,"abstract":"<p><p>Long non-coding RNAs (lncRNAs) play essential roles in various biological processes, such as chromatin remodeling, post-transcriptional regulation, and epigenetic modifications. Despite their critical functions in regulating plant growth, root development, and seed dormancy, the identification of plant lncRNAs remains a challenge due to the scarcity of specific and extensively tested identification methods. Most mainstream machine learning-based methods used for plant lncRNA identification were initially developed using human or other animal datasets, and their accuracy and effectiveness in predicting plant lncRNAs have not been fully evaluated or exploited. To overcome this limitation, we retrained several models, including CPAT, PLEK, and LncFinder, using plant datasets and compared their performance with mainstream lncRNA prediction tools such as CPC2, CNCI, RNAplonc, and LncADeep. Retraining these models significantly improved their performance, and two of the retrained models, LncFinder-plant and CPAT-plant, alongside their ensemble, emerged as the most suitable tools for plant lncRNA identification. This underscores the importance of model retraining in tackling the challenges associated with plant lncRNA identification. Finally, we developed a pipeline (Plant-LncPipe) that incorporates an ensemble of the two best-performing models and covers the entire data analysis process, including reads mapping, transcript assembly, lncRNA identification, classification, and origin, for the efficient identification of lncRNAs in plants. The pipeline, Plant-LncPipe, is available at: https://github.com/xuechantian/Plant-LncRNA-pipline.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 4","pages":"uhae041"},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11024640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissecting the complex genetic basis of pre- and post-harvest traits in <i>Vitis vinifera L</i>. using genome-wide association studies.","authors":"Julian García-Abadillo, Paola Barba, Tiago Carvalho, Viviana Sosa-Zuñiga, Roberto Lozano, Humberto Fanelli Carvalho, Miguel Garcia-Rojas, Erika Salazar, Julio Isidro Y Sánchez","doi":"10.1093/hr/uhad283","DOIUrl":"10.1093/hr/uhad283","url":null,"abstract":"<p><p>Addressing the pressing challenges in agriculture necessitates swift advancements in breeding programs, particularly for perennial crops like grapevines. Moving beyond the traditional biparental quantitative trait loci (QTL) mapping, we conducted a genome-wide association study (GWAS) encompassing 588 <i>Vitis vinifera L.</i> cultivars from a Chilean breeding program, spanning three seasons and testing 13 key yield-related traits. A strong candidate gene, Vitvi11g000454, located on chromosome 11 and related to plant response to biotic and abiotic stresses through jasmonic acid signaling, was associated with berry width and holds potential for enhancing berry size in grape breeding. We also mapped novel QTL associated with post-harvest traits across chromosomes 2, 4, 9, 11, 15, 18, and 19, broadening our grasp on the genetic intricacies dictating fruit post-harvest behavior, including decay, shriveling, and weight loss. Leveraging gene ontology annotations, we drew parallels between traits and scrutinized candidate genes, laying a robust groundwork for future trait-feature identification endeavors in plant breeding. We also highlighted the importance of carefully considering the choice of the response variable in GWAS analyses, as the use of best linear unbiased estimators (BLUEs) corrections in our study may have led to the suppression of some common QTL in grapevine traits. Our results underscore the imperative of pioneering non-destructive evaluation techniques for long-term conservation traits, offering grape breeders and cultivators insights to improve post-harvest table grape quality and minimize waste.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad283"},"PeriodicalIF":0.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring large-scale gene coexpression networks in peach (<i>Prunus persica</i> L.): a new tool for predicting gene function.","authors":"Felipe Pérez de Los Cobos, Beatriz E García-Gómez, Luis Orduña-Rubio, Ignasi Batlle, Pere Arús, José Tomás Matus, Iban Eduardo","doi":"10.1093/hr/uhad294","DOIUrl":"10.1093/hr/uhad294","url":null,"abstract":"<p><p>Peach is a model for <i>Prunus</i> genetics and genomics, however, identifying and validating genes associated to peach breeding traits is a complex task. A gene coexpression network (GCN) capable of capturing stable gene-gene relationships would help researchers overcome the intrinsic limitations of peach genetics and genomics approaches and outline future research opportunities. In this study, we created four GCNs from 604 Illumina RNA-Seq libraries. We evaluated the performance of every GCN in predicting functional annotations using an algorithm based on the 'guilty-by-association' principle. The GCN with the best performance was COO300, encompassing 21 956 genes. To validate its performance predicting gene function, we performed two case studies. In case study 1, we used two genes involved in fruit flesh softening: the endopolygalacturonases <i>PpPG21</i> and <i>PpPG22</i>. Genes coexpressing with both genes were extracted and referred to as melting flesh (MF) network. Finally, we performed an enrichment analysis of MF network and compared the results with the current knowledge regarding peach fruit softening. The MF network mostly included genes involved in cell wall expansion and remodeling, and with expressions triggered by ripening-related phytohormones, such as ethylene, auxin, and methyl jasmonate. In case study 2, we explored potential targets of the anthocyanin regulator PpMYB10.1 by comparing its gene-centered coexpression network with that of its grapevine orthologues, identifying a common regulatory network. These results validated COO300 as a powerful tool for peach and <i>Prunus</i> research. This network, renamed as PeachGCN v1.0, and the scripts required to perform a function prediction analysis are available at https://github.com/felipecobos/PeachGCN.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad294"},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR156b-targeted <i>VvSBP8/13</i> functions downstream of the abscisic acid signal to regulate anthocyanins biosynthesis in grapevine fruit under drought.","authors":"Shuihuan Guo, Meng Zhang, Mingxin Feng, Guipeng Liu, Laurent Torregrosa, Xiaoqing Tao, Ruihua Ren, Yulin Fang, Zhenwen Zhang, Jiangfei Meng, Tengfei Xu","doi":"10.1093/hr/uhad293","DOIUrl":"10.1093/hr/uhad293","url":null,"abstract":"<p><p>Anthocyanins are the primary color components of grapevine berries and wines. In cultivation practices, a moderate water deficit can promote anthocyanin accumulation in red grape skins. Our previous study showed that abscisic acid (ABA) plays a key role in this process. Herein, we identified a microRNA, vv-miR156b, that is generated in grapevine berries in response to drought stress, along with increasing anthocyanin content and biosynthetic structural gene transcripts. In contrast, vv-miR156b short tandem target mimic (STTM) function-loss callus exhibits the opposite phenotype. Results from <i>in vivo</i> and <i>in vitro</i> experiments revealed that the ABA-signaling-regulated transcription factor VvAREB2 binds directly to the ABA-responsive element (ABRE) of the <i>MIR156b</i> promoter and activates miR156b expression. Furthermore, two miR156b downstream targets, <i>VvSBP8</i> and <i>VvSBP13</i>, exhibited reduced grape anthocyanin content in their overexpressors but there was a contrary result in their CRISPR-edited lines, the decrease in anthocyanin content was rescued in miR156b and SBP8/13 double overexpressors. We further demonstrated that both <i>VvSBP8</i> and <i>VvSBP13</i>, encoding transcriptional repressors, displayed sufficient ability to interact with VvMYC1 and VvMYBA1, thereby interfering with MYB-bHLH-WD (MBW) repeat transcriptional complex formation, resulting in the repression of anthocyanin biosynthesis. Our findings demonstrate a direct functional relationship between ABA signaling and the miR156-SBP-MBW complex regulatory module in driving drought-induced anthocyanin accumulation in grape berries.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad293"},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10873574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139900981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA-miRNA analyses reveal the involvement of CsbHLH1 and miR1446a in the regulation of caffeine biosynthesis in <i>Camellia sinensis</i>.","authors":"Qifang Jin, Zhong Wang, Devinder Sandhu, Lan Chen, Chenyu Shao, Fanghuizi Shang, Siyi Xie, Feiyi Huang, Zhenyan Chen, Xiangqin Zhang, Jinyu Hu, Guizhi Liu, Qin Su, Mengdi Huang, Zhonghua Liu, Jianan Huang, Na Tian, Shuoqian Liu","doi":"10.1093/hr/uhad282","DOIUrl":"10.1093/hr/uhad282","url":null,"abstract":"<p><p>Caffeine, a primary flavor component in tea, has been the subject of intense research. With the goal of shedding light on the complex regulatory processes governing caffeine biosynthesis in tea plants, liquid chromatography coupled with mass spectrometry (LC-MS), transcriptomics, and small RNA analyses were employed on diverse tea cultivars such as 'Jianghua Kucha' [including 'Xianghong 3' (XH3H) and 'Kucha 3' (KC3H)], 'Fuding Dabaicha' (FDDB), 'Yaoshan Xiulv' (YSXL), and 'Bixiangzao' (BXZ). The results showed that the caffeine level in 'Jianghua Kucha' was significantly higher than that in other tea plant cultivars. In addition, weighted gene co-expression network analysis indicated that that the <i>CsbHLH1</i> gene might play a pivotal role as a potential hub gene related to the regulation of caffeine biosynthesis. Subcellular localization analysis showed that the CsbHLH1 protein was localized in the nucleus of the cells. Moreover, CsbHLH1 suppresses the transcription of <i>TCS1</i> by binding to the <i>TCS1</i> promoter, as evidenced by a yeast one-hybrid assay, an electrophoretic mobility shift assay, and dual luciferase analysis. In addition, a microRNA, miR1446a, was identified that directly cleaves CsbHLH1, leading to an increase in caffeine levels. Therefore, our findings imply that CsbHLH1 binds to the <i>TCS1</i> promoter (-971 to -1019 bp) to reduce its expression, thereby negatively regulating caffeine biosynthesis. On the other hand, miR1446a enhances the biosynthesis of caffeine by suppressing the expression of <i>CsbHLH1</i>. This work enhances our understanding of the molecular mechanisms of caffeine biosynthesis in tea plants and offers potential directions for manipulating caffeine levels in future tea cultivation.</p>","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad282"},"PeriodicalIF":7.6,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11648165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue culture-free transformation of traditional Chinese medicinal plants with root suckering capability.","authors":"Jinghua Lu, Suhui Lu, Chunli Su, Shuai Deng, Mugui Wang, Huan Tang, Zhunian Wang, Guofu Li, Zhaobo Lang, Jian-Kang Zhu","doi":"10.1093/hr/uhad290","DOIUrl":"10.1093/hr/uhad290","url":null,"abstract":"","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"11 2","pages":"uhad290"},"PeriodicalIF":0.0,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10875980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139906976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}