园艺研究(英文)最新文献_第10页

PsRGL1 negatively regulates chilling- and gibberellin-induced dormancy release by PsF-box1-mediated targeting for proteolytic degradation in tree peony. PsRGL1通过PsF-box1介导的牡丹蛋白水解降解靶向，负调控低温和赤霉素诱导的休眠释放。

园艺研究(英文) Pub Date : 2023-03-13 eCollection Date: 2023-05-01 DOI: 10.1093/hr/uhad044

Linqiang Gao, Demei Niu, Tianyu Chi, Yanchao Yuan, Chunying Liu, Shupeng Gai, Yuxi Zhang

{"title":"PsRGL1 negatively regulates chilling- and gibberellin-induced dormancy release by PsF-box1-mediated targeting for proteolytic degradation in tree peony.","authors":"Linqiang Gao, Demei Niu, Tianyu Chi, Yanchao Yuan, Chunying Liu, Shupeng Gai, Yuxi Zhang","doi":"10.1093/hr/uhad044","DOIUrl":"https://doi.org/10.1093/hr/uhad044","url":null,"abstract":"Tree peony bud endodormancy is a common survival strategy similar to many perennial woody plants in winter, and the activation of the GA signaling pathway is the key to breaking endodormancy. GA signal transduction is involved in many physiological processes. Although the GA-GID1-DELLA regulatory module is conserved in many plants, it has a set of specific components that add complexity to the GA response mechanism. DELLA proteins are key switches in GA signaling. Therefore, there is an urgent need to identify the key DELLA proteins involved in tree peony bud dormancy release. In this study, the prolonged chilling increased the content of endogenously active gibberellins. PsRGL1 among three DELLA proteins was significantly downregulated during chilling- and exogenous GA3-induced bud dormancy release by cell-free degradation assay, and a high level of polyubiquitination was detected. Silencing PsRGL1 accelerated bud dormancy release by increasing the expression of the genes associated with dormancy release, including PsCYCD, PsEBB1, PsEBB3, PsBG6, and PsBG9. Three F-box protein family members responded to chilling and GA3 treatments, resulting in PsF-box1 induction. Yeast two-hybrid and BiFC assays indicated that only PsF-box1 could bind to PsRGL1, and the binding site was in the C-terminal domain. PsF-box1 overexpression promoted dormancy release and upregulated the expression of the dormancy-related genes. In addition, yeast two-hybrid and pull-down assays showed that PsF-box1 also interacted with PsSKP1 to form an E3 ubiquitin ligase. These findings enriched the molecular mechanism of the GA signaling pathway during dormancy release, and enhanced the understanding of tree peony bud endodormancy.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 5","pages":"uhad044"},"PeriodicalIF":0.0,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties. 显微镜和代谢研究揭示了导致辣椒类水果品种表皮开裂的因素。

园艺研究(英文) Pub Date : 2023-02-28 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad036

Ofir Marinov, Gal Nomberg, Sutanni Sarkar, Gulab Chand Arya, Eldad Karavani, Einat Zelinger, Ekaterina Manasherova, Hagai Cohen

{"title":"Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties.","authors":"Ofir Marinov, Gal Nomberg, Sutanni Sarkar, Gulab Chand Arya, Eldad Karavani, Einat Zelinger, Ekaterina Manasherova, Hagai Cohen","doi":"10.1093/hr/uhad036","DOIUrl":"10.1093/hr/uhad036","url":null,"abstract":"The hydrophobic cuticle encasing the fruit skin surface plays critical roles during fruit development and post-harvest. Skin failure often results in the fruit surface cracking and forming a wound-periderm tissue made of suberin and lignin. The factors that make the fruit skin susceptible to cracking have yet to be fully understood. Herein, we investigated two varieties of chili peppers (Capsicum annuum L.), Numex Garnet, whose fruit has intact skin, and Vezena Slatka, whose fruit has cracked skin. Microscopical observations, gas chromatography-mass spectrometry, biochemical and gene expression assays revealed that Vezena Slatka fruit form a thicker cuticle with greater levels of cutin monomers and hydroxycinnamic acids, and highly express key cutin-related genes. The skin of these fruit also had a lower epidermal cell density due to cells with very large perimeters, and highly express genes involved in epidermal cell differentiation. We demonstrate that skin cracking in the Vezena Slatka fruit is accompanied by a spatial accumulation of lignin-like polyphenolic compounds, without the formation of a typical wound-periderm tissues made of suberized cells. Lastly, we establish that skin cracking in chili-type pepper significantly affects fruit quality during post-harvest storage in a temperature-dependent manner. In conclusion, our data highlight cuticle thickness and epidermal cell density as two critical factors determining fruit skin susceptibility to cracking in chili-type pepper fruit.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad036"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41170389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Potato tonoplast sugar transporter 1 controls tuber sugar accumulation during postharvest cold storage. 马铃薯液泡膜糖转运蛋白1在采后冷藏过程中控制块茎糖的积累。

园艺研究(英文) Pub Date : 2023-02-28 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad035

Tengfei Liu, Md Abu Kawochar, Shahnewaz Begum, Enshuang Wang, Tingting Zhou, Shenglin Jing, Tiantian Liu, Liu Yu, Bihua Nie, Botao Song

{"title":"Potato tonoplast sugar transporter 1 controls tuber sugar accumulation during postharvest cold storage.","authors":"Tengfei Liu, Md Abu Kawochar, Shahnewaz Begum, Enshuang Wang, Tingting Zhou, Shenglin Jing, Tiantian Liu, Liu Yu, Bihua Nie, Botao Song","doi":"10.1093/hr/uhad035","DOIUrl":"10.1093/hr/uhad035","url":null,"abstract":"Cold-induced sweetening (CIS), the undesirable sugar accumulation in cold-stored potato (Solanum tuberosum L.) tubers, is a severe postharvest issue in the potato processing industry. Although the process of sucrose hydrolysis by vacuolar invertase during potato CIS is well understood, there is limited knowledge about the transportation of sucrose from the cytosol to the vacuole during postharvest cold storage. Here, we report that among the three potato tonoplast sugar transporters (TSTs), StTST1 exhibits the highest expression in tubers during postharvest cold storage. Subcellular localization analysis demonstrates that StTST1 is a tonoplast-localized protein. StTST1 knockdown decreases reducing sugar accumulation in tubers during low-temperature storage. Compared to wild-type, potato chips produced from StTST1-silenced tubers displayed significantly lower acrylamide levels and lighter color after cold storage. Transcriptome analysis manifests that suppression of StTST1 promotes starch synthesis and inhibits starch degradation in cold-stored tubers. We further establish that the increased sucrose content in the StTST1-silenced tubers might cause a decrease in the ABA content, thereby inhibiting the ABA-signaling pathway. We demonstrate that the down-regulation of β-amylase StBAM1 in StTST1-silenced tubers might be directly controlled by ABA-responsive element-binding proteins (AREBs). Altogether, we have shown that StTST1 plays a critical role in sugar accumulation and starch metabolism regulation during postharvest cold storage. Thus, our findings provide a new strategy to improve the frying quality of cold-stored tubers and reduce the acrylamide content in potato chips.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad035"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41145257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of clade-wide putative cis-regulatory elements from conserved non-coding sequences in Cucurbitaceae genomes. 从葫芦科基因组中保守的非编码序列中鉴定分支范围内推定的顺式调控元件。

园艺研究(英文) Pub Date : 2023-02-28 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad038

Hongtao Song, Qi Wang, Zhonghua Zhang, Kui Lin, Erli Pang

{"title":"Identification of clade-wide putative cis-regulatory elements from conserved non-coding sequences in Cucurbitaceae genomes.","authors":"Hongtao Song, Qi Wang, Zhonghua Zhang, Kui Lin, Erli Pang","doi":"10.1093/hr/uhad038","DOIUrl":"10.1093/hr/uhad038","url":null,"abstract":"Cis-regulatory elements regulate gene expression and play an essential role in the development and physiology of organisms. Many conserved non-coding sequences (CNSs) function as cis-regulatory elements. They control the development of various lineages. However, predicting clade-wide cis-regulatory elements across several closely related species remains challenging. Based on the relationship between CNSs and cis-regulatory elements, we present a computational approach that predicts the clade-wide putative cis-regulatory elements in 12 Cucurbitaceae genomes. Using 12-way whole-genome alignment, we first obtained 632 112 CNSs in Cucurbitaceae. Next, we identified 16 552 Cucurbitaceae-wide cis-regulatory elements based on collinearity among all 12 Cucurbitaceae plants. Furthermore, we predicted 3 271 potential regulatory pairs in the cucumber genome, of which 98 were verified using integrative RNA sequencing and ChIP sequencing datasets from samples collected during various fruit development stages. The CNSs, Cucurbitaceae-wide cis-regulatory elements, and their target genes are accessible at http://cmb.bnu.edu.cn/cisRCNEs_cucurbit/. These elements are valuable resources for functionally annotating CNSs and their regulatory roles in Cucurbitaceae genomes.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad038"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41174381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large-scale population structure and genetic architecture of agronomic traits of garlic. 大蒜农艺性状的大规模群体结构和遗传结构。

园艺研究(英文) Pub Date : 2023-02-22 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad034

Huixia Jia, Qing Zhao, Jiangping Song, Xiaohui Zhang, Wenlong Yang, Zhenzhen Du, Yue Zhu, Haiping Wang

{"title":"Large-scale population structure and genetic architecture of agronomic traits of garlic.","authors":"Huixia Jia, Qing Zhao, Jiangping Song, Xiaohui Zhang, Wenlong Yang, Zhenzhen Du, Yue Zhu, Haiping Wang","doi":"10.1093/hr/uhad034","DOIUrl":"10.1093/hr/uhad034","url":null,"abstract":"Garlic, an asexually propagated crop, is the second important bulb crop after the onion and is used as a vegetable and medicinal plant. Abundant and diverse garlic resources have been formed over thousands of years of cultivation. However, genome variation, population structure and genetic architecture of garlic agronomic traits were still not well elucidated. Here, 1 100 258 single nucleotide polymorphisms (SNPs) were identified using genotyping-by-sequencing in 606 garlic accessions collected from 43 countries. Population structure, principal component and phylogenetic analysis showed that these accessions were divided into five subpopulations. Twenty agronomic traits, including above-ground growth traits, bulb-related and bolt-related traits in two consecutive years were implemented in a genome-wide association study. In total, 542 SNPs were associated with these agronomic traits, among which 188 SNPs were repeatedly associated with more than two traits. One SNP (chr6: 1896135972) was repeatedly associated with ten traits. These associated SNPs were located within or near 858 genes, 56 of which were transcription factors. Interestingly, one non-synonymous SNP (Chr4: 166524085) in ribosomal protein S5 was repeatedly associated with above-ground growth and bulb-related traits. Additionally, gene ontology enrichment analysis of candidate genes for genomic selection regions between complete-bolting and non-bolting accessions showed that these genes were significantly enriched in 'vegetative to reproductive phase transition of meristem', 'shoot system development', 'reproductive process', etc. These results provide valuable information for the reliable and efficient selection of candidate genes to achieve garlic genetic improvement and superior varieties.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad034"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41170388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The genomic and epigenetic footprint of local adaptation to variable climates in kiwifruit. 猕猴桃局部适应多变气候的基因组和表观遗传学足迹。

园艺研究(英文) Pub Date : 2023-02-21 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad031

Xu Zhang, Rui Guo, Ruinan Shen, Jacob B Landis, Quan Jiang, Fang Liu, Hengchang Wang, Xiaohong Yao

{"title":"The genomic and epigenetic footprint of local adaptation to variable climates in kiwifruit.","authors":"Xu Zhang, Rui Guo, Ruinan Shen, Jacob B Landis, Quan Jiang, Fang Liu, Hengchang Wang, Xiaohong Yao","doi":"10.1093/hr/uhad031","DOIUrl":"10.1093/hr/uhad031","url":null,"abstract":"A full understanding of adaptive genetic variation at the genomic level will help address questions of how organisms adapt to diverse climates. Actinidia eriantha is a shade-tolerant species, widely distributed in the southern tropical region of China, occurring in spatially heterogeneous environments. In the present study we combined population genomic, epigenomic, and environmental association analyses to infer population genetic structure and positive selection across a climatic gradient, and to assess genomic offset to climatic change for A. eriantha. The population structure is strongly shaped by geography and influenced by restricted gene flow resulting from isolation by distance due to habitat fragmentation. In total, we identified 102 outlier loci and annotated 455 candidate genes associated with the genomic basis of climate adaptation, which were enriched in functional categories related to development processes and stress response; both temperature and precipitation are important factors driving adaptive variation. In addition to single-nucleotide polymorphisms (SNPs), a total of 27 single-methylation variants (SMVs) had significant correlation with at least one of four climatic variables and 16 SMVs were located in or adjacent to genes, several of which were predicted to be involved in plant response to abiotic or biotic stress. Gradient forest analysis indicated that the central/east populations were predicted to be at higher risk of future population maladaptation under climate change. Our results demonstrate that local climate factors impose strong selection pressures and lead to local adaptation. Such information adds to our understanding of adaptive mechanisms to variable climates revealed by both population genome and epigenome analysis.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad031"},"PeriodicalIF":0.0,"publicationDate":"2023-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The RING-H2 gene LdXERICO plays a negative role in dormancy release regulated by low temperature in Lilium davidii var. unicolor. RING-H2基因LdXERICO在低温调控下对独花百合休眠解除起着负调控作用。

园艺研究(英文) Pub Date : 2023-02-20 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad030

Xinyue Fan, Xiaoman Zou, Linlan Fu, Yue Yang, Min Li, Chunxia Wang, Hongmei Sun

{"title":"The RING-H2 gene LdXERICO plays a negative role in dormancy release regulated by low temperature in Lilium davidii var. unicolor.","authors":"Xinyue Fan, Xiaoman Zou, Linlan Fu, Yue Yang, Min Li, Chunxia Wang, Hongmei Sun","doi":"10.1093/hr/uhad030","DOIUrl":"10.1093/hr/uhad030","url":null,"abstract":"Dormancy regulation is the basis of the sustainable development of the lily industry. Therefore, basic research on lily dormancy is crucial for innovation in lily cultivation and breeding. Previous studies revealed that dormancy release largely depends on abscisic acid (ABA) degradation. However, the key genes and potential regulatory network remain unclear. We used exogenous ABA and ABA inhibitors to elucidate the effect of ABA on lily dormancy. Based on the results of weighted gene coexpression network analysis (WGCNA), the hub gene LdXERICO was identified in modules highly related to endogenous ABA, and a large number of coexpressed genes were identified. LdXERICO was induced by exogenous ABA and expressed at higher levels in tissues with vigorous physiological activity. Silencing LdXERICO increased the low-temperature sensitivity of bulblets and accelerated bulblet sprouting. LdXERICO rescued the ABA insensitivity of xerico mutants during seed germination in Arabidopsis, suggesting that it promotes seed dormancy and supporting overexpression studies on lily bulblets. The significant increase in ABA levels in transgenic Arabidopsis expressing LdXERICO indicated that LdXERICO played a role by promoting ABA synthesis. We generated three transgenic lines by overexpressing LdICE1 in Arabidopsis thaliana and showed that, in contrast to LdXERICO, LdICE1 positively regulated dormancy release. Finally, qRT-PCR confirmed that LdXERICO was epistatic to LdICE1 for dormancy release. We propose that LdXERICO, an essential gene in dormancy regulation through the ABA-related pathway, has a complex regulatory network involving temperature signals. This study provides a theoretical basis for further exploring the mechanism of bulb dormancy release.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad030"},"PeriodicalIF":0.0,"publicationDate":"2023-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10548414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41160586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in kumquat. 蛋白酶抑制剂ASP通过抑制金桔中蛋白质的降解来增强其抗冻性。

园艺研究(英文) Pub Date : 2023-02-16 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad023

Hua Yang, Ke-Wei Qiao, Jin-Jing Teng, Jia-Bei Chen, Ying-Li Zhong, Li-Qun Rao, Xing-Yao Xiong, Huang Li

{"title":"Protease inhibitor ASP enhances freezing tolerance by inhibiting protein degradation in kumquat.","authors":"Hua Yang, Ke-Wei Qiao, Jin-Jing Teng, Jia-Bei Chen, Ying-Li Zhong, Li-Qun Rao, Xing-Yao Xiong, Huang Li","doi":"10.1093/hr/uhad023","DOIUrl":"https://doi.org/10.1093/hr/uhad023","url":null,"abstract":"Cold acclimation is a complex biological process leading to the development of freezing tolerance in plants. In this study, we demonstrated that cold-induced expression of protease inhibitor FmASP in a Citrus-relative species kumquat [Fortunella margarita (Lour.) Swingle] contributes to its freezing tolerance by minimizing protein degradation. Firstly, we found that only cold-acclimated kumquat plants, despite extensive leaf cellular damage during freezing, were able to resume their normal growth upon stress relief. To dissect the impact of cold acclimation on this anti-freezing performance, we conducted protein abundance assays and quantitative proteomic analysis of kumquat leaves subjected to cold acclimation (4°C), freezing treatment (-10°C) and post-freezing recovery (25°C). FmASP (Against Serine Protease) and several non-specific proteases were identified as differentially expressed proteins induced by cold acclimation and associated with stable protein abundance throughout the course of low-temperature treatment. FmASP was further characterized as a robust inhibitor of multiple proteases. In addition, heterogeneous expression of FmASP in Arabidopsis confirmed its positive role in freezing tolerance. Finally, we proposed a working model of FmASP and illustrated how this extracellular-localized protease inhibitor protects proteins from degradation, thereby maintaining essential cellular function for post-freezing recovery. These findings revealed the important role of protease inhibition in freezing response and provide insights on how this role may help develop new strategies to enhance plant freezing tolerance.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad023"},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41169621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A petunia transcription factor, PhOBF1, regulates flower senescence by modulating gibberellin biosynthesis. 矮牵牛转录因子PhOBF1通过调节赤霉素的生物合成来调节花朵衰老。

园艺研究(英文) Pub Date : 2023-02-16 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad022

Xiaotong Ji, Ziwei Xin, Yanping Yuan, Meiling Wang, Xinyi Lu, Jiaqi Li, Yanlong Zhang, Lixin Niu, Cai-Zhong Jiang, Daoyang Sun

{"title":"A petunia transcription factor, PhOBF1, regulates flower senescence by modulating gibberellin biosynthesis.","authors":"Xiaotong Ji, Ziwei Xin, Yanping Yuan, Meiling Wang, Xinyi Lu, Jiaqi Li, Yanlong Zhang, Lixin Niu, Cai-Zhong Jiang, Daoyang Sun","doi":"10.1093/hr/uhad022","DOIUrl":"https://doi.org/10.1093/hr/uhad022","url":null,"abstract":"Flower senescence is commonly enhanced by the endogenous hormone ethylene and suppressed by the gibberellins (GAs) in plants. However, the detailed mechanisms for the antagonism of these hormones during flower senescence remain elusive. In this study, we characterized one up-regulated gene PhOBF1, belonging to the basic leucine zipper transcription factor family, in senescing petals of petunia (Petunia hybrida). Exogenous treatments with ethylene and GA3 provoked a dramatic increase in PhOBF1 transcripts. Compared with wild-type plants, PhOBF1-RNAi transgenic petunia plants exhibited shortened flower longevity, while overexpression of PhOBF1 resulted in delayed flower senescence. Transcript abundances of two senescence-related genes PhSAG12 and PhSAG29 were higher in PhOBF1-silenced plants but lower in PhOBF1-overexpressing plants. Silencing and overexpression of PhOBF1 affected expression levels of a few genes involved in the GA biosynthesis and signaling pathways, as well as accumulation levels of bioactive GAs GA1 and GA3. Application of GA3 restored the accelerated petal senescence to normal levels in PhOBF1-RNAi transgenic petunia lines, and reduced ethylene release and transcription of three ethylene biosynthetic genes PhACO1, PhACS1, and PhACS2. Moreover, PhOBF1 was observed to specifically bind to the PhGA20ox3 promoter containing a G-box motif. Transient silencing of PhGA20ox3 in petunia plants through tobacco rattle virus-based virus-induced gene silencing method led to accelerated corolla senescence. Our results suggest that PhOBF1 functions as a negative regulator of ethylene-mediated flower senescence by modulating the GA production.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad022"},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41162898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating colorful carrot germplasm through metabolic engineering of betalains pigments. 通过β赖氨酸色素的代谢工程产生丰富多彩的胡萝卜种质。

IF 7.6

园艺研究(英文) Pub Date : 2023-02-14 eCollection Date: 2023-04-01 DOI: 10.1093/hr/uhad024

Yuan-Jie Deng, Ao-Qi Duan, Hui Liu, Ya-Hui Wang, Rong-Rong Zhang, Zhi-Sheng Xu, Ai-Sheng Xiong

{"title":"Generating colorful carrot germplasm through metabolic engineering of betalains pigments.","authors":"Yuan-Jie Deng, Ao-Qi Duan, Hui Liu, Ya-Hui Wang, Rong-Rong Zhang, Zhi-Sheng Xu, Ai-Sheng Xiong","doi":"10.1093/hr/uhad024","DOIUrl":"10.1093/hr/uhad024","url":null,"abstract":"Betalains are tyrosine-derived plant pigments exclusively found in the Caryophyllales order and some higher fungi and generally classified into two groups: red-violet betacyanins and yellow-orange betaxanthins. Betalains attract great scientific and economic interest because of their relatively simple biosynthesis pathway, attractive colors and health-promoting properties. Co-expressing two core genes BvCYP76AD1 and BvDODA1 with or without a glycosyltransferase gene MjcDOPA5GT allowed the engineering of carrot (an important taproot vegetable) to produce a palette of unique colors. The highest total betalains content, 943.2 μg·g-1 DW, was obtained in carrot taproot transformed with p35S:RUBY which produces all of the necessary enzymes for betalains synthesis. Root-specific production of betalains slightly relieved tyrosine consumption revealing the possible bottleneck in betalains production. Furthermore, a unique volcano-like phenotype in carrot taproot cross-section was created by vascular cambium-specific production of betalains. The betalains-fortified carrot in this study is thus anticipated to be used as functional vegetable and colorful carrot germplasm in breeding to promote health.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 4","pages":"uhad024"},"PeriodicalIF":7.6,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10541523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41169609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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