园艺研究(英文)最新文献_第9页

RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant. RNA测序分析揭示了参与茄子不同果皮颜色形成的新基因。

园艺研究(英文) Pub Date : 2023-09-04 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad181

Jing Li, Senlin Jiang, Guobin Yang, Yanwei Xu, Lujun Li, Fengjuan Yang

{"title":"RNA-sequencing analysis reveals novel genes involved in the different peel color formation in eggplant.","authors":"Jing Li, Senlin Jiang, Guobin Yang, Yanwei Xu, Lujun Li, Fengjuan Yang","doi":"10.1093/hr/uhad181","DOIUrl":"10.1093/hr/uhad181","url":null,"abstract":"Eggplant (Solanum melongena L.) is a highly nutritious vegetable. Here, the molecular mechanism of color formation in eggplants was determined using six eggplant cultivars with different peel colors and two SmMYB113-overexpressing transgenic eggplants with a purple peel and pulp. Significant differentially expressed genes (DEGs) were identified by RNA-sequencing analysis using the following criteria: log2(sample1/sample2) ≥ 0.75 and q-value ≤ 0.05. Two analytical strategies were used to identify genes related to the different peel color according to the peel color, flavonoids content, delphinidins/flavonoids ratio, and the content of anthocyanins. Finally, 27 novel genes were identified to be related to the color difference among eggplant peels and 32 novel genes were identified to be related to anthocyanin biosynthesis and regulated by SmMYB113. Venn analysis revealed that SmCytb5, SmGST, SmMATE, SmASAT3, and SmF3'5'M were shared among both sets of novel genes. Transient expression assay in tobacco suggested that these five genes were not sufficient for inducing anthocyanin biosynthesis alone, but they play important roles in anthocyanin accumulation in eggplant peels. Yeast one-hybrid, electrophoretic mobility shift assay and dual-luciferase assays indicated that the expression of the five genes could be directly activated by SmMYB113 protein. Finally, a regulatory model for the mechanism of color formation in eggplant was proposed. Overall, the results of this study provide useful information that enhances our understanding of the molecular mechanism underlying the different color formation in eggplant.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 10","pages":"uhad181"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54232454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The high-resolution three-dimensional (3D) chromatin map of the tea plant (Camellia sinensis). 茶树高分辨率三维染色质图谱。

园艺研究(英文) Pub Date : 2023-09-01 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad179

Weilong Kong, Jiaxin Yu, Jingjing Yang, Yanbing Zhang, Xingtan Zhang

引用次数: 0

Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator? 甲基化在春化和光周期途径中的作用：一种潜在的开花调节剂？

园艺研究(英文) Pub Date : 2023-08-29 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad174

Meimei Shi, Chunlei Wang, Peng Wang, Fahong Yun, Zhiya Liu, Fujin Ye, Lijuan Wei, Weibiao Liao

{"title":"Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?","authors":"Meimei Shi, Chunlei Wang, Peng Wang, Fahong Yun, Zhiya Liu, Fujin Ye, Lijuan Wei, Weibiao Liao","doi":"10.1093/hr/uhad174","DOIUrl":"10.1093/hr/uhad174","url":null,"abstract":"Recognized as a pivotal developmental transition, flowering marks the continuation of a plant's life cycle. Vernalization and photoperiod are two major flowering pathways orchestrating numerous florigenic signals. Methylation, including histone, DNA and RNA methylation, is one of the recent foci in plant development. Considerable studies reveal that methylation seems to show an increasing potential regulatory role in plant flowering via altering relevant gene expression without altering the genetic basis. However, little has been reviewed about whether and how methylation acts on vernalization- and photoperiod-induced flowering before and after FLOWERING LOCUS C (FLC) reactivation, what role RNA methylation plays in vernalization- and photoperiod-induced flowering, how methylation participates simultaneously in both vernalization- and photoperiod-induced flowering, the heritability of methylation memory under the vernalization/photoperiod pathway, and whether and how methylation replaces vernalization/photoinduction to regulate flowering. Our review provides insight about the crosstalk among the genetic control of the flowering gene network, methylation (methyltransferases/demethylases) and external signals (cold, light, sRNA and phytohormones) in vernalization and photoperiod pathways. The existing evidence that RNA methylation may play a potential regulatory role in vernalization- and photoperiod-induced flowering has been gathered and represented for the first time. This review speculates about and discusses the possibility of substituting methylation for vernalization and photoinduction to promote flowering. Current evidence is utilized to discuss the possibility of future methylation reagents becoming flowering regulators at the molecular level.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 10","pages":"uhad174"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VvWRKY5 enhances white rot resistance in grape by promoting the jasmonic acid pathway. VvWRKY5通过促进茉莉酸途径增强葡萄对白腐病的抗性。

园艺研究(英文) Pub Date : 2023-08-29 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad172

Zhen Zhang, Changyue Jiang, Cui Chen, Kai Su, Hong Lin, Yuhui Zhao, Yinshan Guo

{"title":"VvWRKY5 enhances white rot resistance in grape by promoting the jasmonic acid pathway.","authors":"Zhen Zhang, Changyue Jiang, Cui Chen, Kai Su, Hong Lin, Yuhui Zhao, Yinshan Guo","doi":"10.1093/hr/uhad172","DOIUrl":"10.1093/hr/uhad172","url":null,"abstract":"Grape white rot is a disease caused by Coniella diplodiella (Speg.) Sacc. (Cd) can drastically reduce the production and quality of grape (Vitis vinifera). WRKY transcription factors play a vital role in the regulation of plant resistance to pathogens, but their functions in grape white rot need to be further explored. Here, we found that the expression of the WRKY IIe subfamily member VvWRKY5 was highly induced by Cd infection and jasmonic acid (JA) treatment. Transient injection and stable overexpression (in grape calli and Arabidopsis) demonstrated that VvWRKY5 positively regulated grape resistance to white rot. We also determined that VvWRKY5 regulated the JA response by directly binding to the promoters of VvJAZ2 (a JA signaling suppressor) and VvMYC2 (a JA signaling activator), thereby inhibiting and activating the transcription of VvJAZ2 and VvMYC2, respectively. Furthermore, the interaction between VvJAZ2 and VvWRKY5 enhanced the suppression and promotion of VvJAZ2 and VvMYC2 activities by VvWRKY5, respectively. When VvWRKY5 was overexpressed in grape, JA content was also increased. Overall, our results suggested that VvWRKY5 played a key role in regulating JA biosynthesis and signal transduction as well as enhancing white rot resistance in grape. Our results also provide theoretical guidance for the development of elite grape cultivars with enhanced pathogen resistance.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 10","pages":"uhad172"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A gap-free reference genome reveals structural variations associated with flowering time in rapeseed (Brassica napus). 无间隙参考基因组揭示了油菜（Brassica napus）与开花时间相关的结构变异。

园艺研究(英文) Pub Date : 2023-08-29 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad171

Bao Li, Qian Yang, Lulu Yang, Xing Zhou, Lichao Deng, Liang Qu, Dengli Guo, Rongkui Hui, Yiming Guo, Xinhong Liu, Tonghua Wang, Lianyi Fan, Mei Li, Mingli Yan

{"title":"A gap-free reference genome reveals structural variations associated with flowering time in rapeseed (Brassica napus).","authors":"Bao Li, Qian Yang, Lulu Yang, Xing Zhou, Lichao Deng, Liang Qu, Dengli Guo, Rongkui Hui, Yiming Guo, Xinhong Liu, Tonghua Wang, Lianyi Fan, Mei Li, Mingli Yan","doi":"10.1093/hr/uhad171","DOIUrl":"10.1093/hr/uhad171","url":null,"abstract":"Allopolyploid oilseed rape (Brassica napus) is an important oil crop and vegetable. However, the latest version of its reference genome, with collapsed duplications, gaps, and other issues, prevents comprehensive genomic analysis. Herein, we report a gap-free assembly of the rapeseed cv. Xiang5A genome using a combination of ONT (Oxford Nanopore Technologies) ultra-long reads, PacBio high-fidelity reads, and Hi-C datasets. It includes gap-free assemblies of all 19 chromosomes and telomere-to-telomere assemblies of eight chromosomes. Compared with previously published genomes of B. napus, our gap-free genome, with a contig N50 length of 50.70 Mb, has complete assemblies of 9 of 19 chromosomes without manual intervention, and greatly improves contiguity and completeness, thereby representing the highest quality genome assembly to date. Our results revealed that B. napus Xiang5A underwent nearly complete triplication and allotetraploidy relative to Arabidopsis thaliana. Using the gap-free assembly, we found that 917 flowering-related genes were affected by structural variation, including BnaA03.VERNALIZATION INSENSITIVE 3 and BnaC04.HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1. These genes may play crucial roles in regulating flowering time and facilitating the adaptation of Xiang5A in the Yangtze River Basin of China. This reference genome provides a valuable genetic resource for rapeseed functional genomic studies and breeding.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 10","pages":"uhad171"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transcriptomic and genetic approaches reveal that low-light-induced disease susceptibility is related to cellular oxidative stress in tomato. 转录组学和遗传学方法表明，低光诱导的番茄疾病易感性与细胞氧化应激有关。

园艺研究(英文) Pub Date : 2023-08-29 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad173

Qian Luo, Jiao Wang, Ping Wang, Xiao Liang, Jianxin Li, Changqi Wu, Hanmo Fang, Shuting Ding, Shujun Shao, Kai Shi

{"title":"Transcriptomic and genetic approaches reveal that low-light-induced disease susceptibility is related to cellular oxidative stress in tomato.","authors":"Qian Luo, Jiao Wang, Ping Wang, Xiao Liang, Jianxin Li, Changqi Wu, Hanmo Fang, Shuting Ding, Shujun Shao, Kai Shi","doi":"10.1093/hr/uhad173","DOIUrl":"https://doi.org/10.1093/hr/uhad173","url":null,"abstract":"The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security, as it frequently results in significant yield losses. However, the underlying mechanisms of the effect of low light on plant defense are still poorly understood. Here, using an RNA-seq approach, we found that the susceptibility of tomato to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) under low light was associated with the oxidation-reduction process. Low light conditions exacerbated Pst DC3000-induced reactive oxygen species (ROS) accumulation and protein oxidation. Analysis of gene expression and enzyme activity of ascorbate peroxidase 2 (APX2) and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light, whereas these genes and their associated enzyme activities were not responsive to pathogen inoculation under low light. Additionally, the reduced ascorbate to dehydroascorbate (AsA/DHA) ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation. Furthermore, the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions. Notably, this increased susceptibility could be significantly reduced by exogenous AsA treatment. Collectively, our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants. This study sheds light on the intricate relationship between light conditions, oxidative stress, and plant defense responses, and may pave the way for improved crop protection strategies in low light environments.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 10","pages":"uhad173"},"PeriodicalIF":0.0,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41241310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Lilium spp.). LhWRKY44在促进亚洲杂交百合花青素积累中的多方面作用。

园艺研究(英文) Pub Date : 2023-08-22 eCollection Date: 2023-09-01 DOI: 10.1093/hr/uhad167

Mengmeng Bi, Rui Liang, Jiawen Wang, Yuxiao Qu, Xin Liu, Yuwei Cao, Guoren He, Yue Yang, Panpan Yang, Leifeng Xu, Jun Ming

{"title":"Multifaceted roles of LhWRKY44 in promoting anthocyanin accumulation in Asiatic hybrid lilies (Lilium spp.).","authors":"Mengmeng Bi, Rui Liang, Jiawen Wang, Yuxiao Qu, Xin Liu, Yuwei Cao, Guoren He, Yue Yang, Panpan Yang, Leifeng Xu, Jun Ming","doi":"10.1093/hr/uhad167","DOIUrl":"10.1093/hr/uhad167","url":null,"abstract":"The Asiatic hybrid lily (Lilium spp.) is a horticultural crop with high commercial value and diverse anthocyanin pigmentation patterns. However, the regulatory mechanism underlying lily flower color has been largely unexplored. Here, we identified a WRKY transcription factor from lily tepals, LhWRKY44, whose expression was closely associated with anthocyanin accumulation. Functional verification indicated that LhWRKY44 positively regulated anthocyanin accumulation. LhWRKY44 physically interacted with LhMYBSPLATTER and directly bound to the LhMYBSPLATTER promoter, which enhanced the effect of the LhMYBSPLATTER-LhbHLH2 MBW complex activator on anthocyanin accumulation. Moreover, EMSA and dual-luciferase assays revealed that LhWRKY44 activated and bound to the promoters of gene LhF3H and the intracellular anthocyanin-related glutathione S-transferase gene LhGST. Interestingly, our further results showed that LhWRKY44 participated in light and drought-induced anthocyanin accumulation, and improved the drought tolerance in lily via activating stress-related genes. These results generated a multifaceted regulatory mechanism for the LhWRKY44-meditaed enhancement by the environmental signal pathway of anthocyanin accumulation and expanded our understanding of the WRKY-mediated transcriptional regulatory hierarchy modulating anthocyanin accumulation in Asiatic hybrid lilies.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 9","pages":"uhad167"},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10535013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41162669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs. 植物中有效的大片段缺失：双对sgRNA比双sgRNA更好。

园艺研究(英文) Pub Date : 2023-08-22 eCollection Date: 2023-10-01 DOI: 10.1093/hr/uhad168

Guoning Zhu, Lingling Zhang, Liqun Ma, Qing Liu, Kejian Wang, Jinyan Li, Guiqin Qu, Benzhong Zhu, Daqi Fu, Yunbo Luo, Hongliang Zhu

引用次数: 0

The parallel biosynthesis routes of hyperoside from naringenin in Hypericum monogynum. 金丝桃柚皮素中金丝桃苷的平行生物合成途径。

园艺研究(英文) Pub Date : 2023-08-17 eCollection Date: 2023-09-01 DOI: 10.1093/hr/uhad166

Yingying Wang, Zhirong Cui, Qianqian Li, Shuai Zhang, Yongyi Li, Xueyan Li, Lingyi Kong, Jun Luo

{"title":"The parallel biosynthesis routes of hyperoside from naringenin in Hypericum monogynum.","authors":"Yingying Wang, Zhirong Cui, Qianqian Li, Shuai Zhang, Yongyi Li, Xueyan Li, Lingyi Kong, Jun Luo","doi":"10.1093/hr/uhad166","DOIUrl":"https://doi.org/10.1093/hr/uhad166","url":null,"abstract":"Hyperoside is a bioactive flavonoid galactoside in both medicinal and edible plants. It plays an important physiological role in the growth of flower buds. However, the hyperoside biosynthesis pathway has not been systematically elucidated in plants, including its original source, Hypericaceae. Our group found abundant hyperoside in the flower buds of Hypericum monogynum, and we sequenced its transcriptome to study the biosynthetic mechanism of hyperoside. After gene screening and functional verification, four kinds of key enzymes were identified. Specifically, HmF3Hs (flavanone 3-hydroxylases) and HmFLSs (flavonol synthases) could catalyze flavanones into dihydroflavonols, as well as catalyzing dihydroflavonols into flavonols. HmFLSs could also convert flavanones into flavonols and flavones with varying efficiencies. HmF3'H (flavonoid 3'-hydroxylase) was found to act broadly on 4'-hydroxyl flavonoids to produce 3',4'-diydroxylated flavanones, dihydroflavonols, flavonols, and flavones. HmGAT (flavonoid 3-O-galactosyltransferase) would transform flavonols into the corresponding 3-O-galactosides, including hyperoside. The parallel hyperoside biosynthesis routes were thus depicted, one of which was successfully reconstructed in Escherichia coli BL21(DE3) by feeding naringenin, resulting in a hyperoside yield of 25 mg/l. Overall, this research not only helped us understand the interior catalytic mechanism of hyperoside in H. monogynum concerning flower development and bioactivity, but also provided valuable insights into these enzyme families.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"10 9","pages":"uhad166"},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10506691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41177558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benchmarking gene set of gymnosperms for assessing genome and annotation completeness in BUSCO. 用于评估BUSCO基因组和注释完整性的裸子植物基准基因集。

园艺研究(英文) Pub Date : 2023-08-17 eCollection Date: 2023-09-01 DOI: 10.1093/hr/uhad165

Jun-Jie Wu, Yu-Wei Han, Chen-Feng Lin, Jing Cai, Yun-Peng Zhao

引用次数: 0