IF 9.5

园艺研究(英文) Pub Date : 2026-10-05 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag385

引用次数: 0

Correction to: Establishing a biomarker set for the diagnosis of apple replant disease using qPCR. 修正：利用qPCR技术建立苹果再植病诊断的生物标记集。

IF 9.5

园艺研究(英文) Pub Date : 2026-10-05 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag391

引用次数: 0

Correction to: VaWRKY65 contributes to cold tolerance through dual requaltion of soluble sugar accumulation and reactive oxygen species scavenging in Vitis amurensis. 修正：vwrky65通过对葡萄中可溶性糖积累和活性氧清除的双重重估，有助于耐寒性。

IF 9.5

园艺研究(英文) Pub Date : 2026-07-29 eCollection Date: 2026-07-01 DOI: 10.1093/hr/uhag317

引用次数: 0

AcGLK2 involves anthocyanin biosynthesis via bidirectionally modulating expression of MYB transcription factors in Actinidia chinensis. AcGLK2通过双向调节猕猴桃MYB转录因子的表达参与花青素的生物合成。

IF 9.5

园艺研究(英文) Pub Date : 2026-07-01 DOI: 10.1093/hr/uhag105

Lili Zhao, Mingzhu Tang, Guocheng Wang, Ping Wang, Zhongru Cui, Yiwei Zhao, Song He, Pengpeng Zheng, Junyang Yue, Songhu Wang, Yongsheng Liu, Lihuan Wang

{"title":"AcGLK2 involves anthocyanin biosynthesis via bidirectionally modulating expression of MYB transcription factors in Actinidia chinensis.","authors":"Lili Zhao, Mingzhu Tang, Guocheng Wang, Ping Wang, Zhongru Cui, Yiwei Zhao, Song He, Pengpeng Zheng, Junyang Yue, Songhu Wang, Yongsheng Liu, Lihuan Wang","doi":"10.1093/hr/uhag105","DOIUrl":"10.1093/hr/uhag105","url":null,"abstract":"The GOLDEN2-LIKE (GLK) gene family, known for its role in chloroplast development, has recently been implicated in involvement of anthocyanin biosynthesis in kiwifruit (Actinidia spp.), but the underlying regulatory mechanism remains unclear. Here we report the characterization of a kiwifruit GLK homolog AcGLK2 in regulating anthocyanin accumulation. We found that expression of AcGLK2 is much higher exclusively in the red pigment-accumulated fruit tissue. Overexpression of AcGLK2 in Arabidopsis and kiwifruit significantly enhanced anthocyanin content, whereas its RNAi-mediated silencing compromised anthocyanin accumulation. RNA-Seq analysis revealed significant upregulation of many structural genes and transcription factors (TFs) associated with the flavonoid pathway in AcGLK2-overexpressing kiwifruit. ChIP-Seq analysis indicated that AcGLK2 directly binds to AcMYB5 and AcTRY. We showed AcMYB5, an R2R3-MYB TF, promotes anthocyanin accumulation by interacting with the bHLH protein AcbHLH42, while AcTRY, an R3-MYB protein, competitively inhibits the interaction between key MYBs and AcbHLH42, thereby repressing anthocyanin biosynthesis. Transgenic and molecular assays in tobacco, tomato, and kiwifruit demonstrated that AcGLK2 positively participates into regulation of anthocyanin accumulation through transcriptionally activating AcMYB5 expression and concomitantly suppressing AcTRY expression. This study reveals the dual regulatory mechanism of AcGLK2 in anthocyanin biosynthesis, broadening the understanding of GLK gene functions and providing a valuable genetic resource for molecular breeding of nutritional quality in kiwifruit and other crops.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 7","pages":"uhag105"},"PeriodicalIF":9.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148364059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient gene editing system for Eustoma grandiflorum based on somatic embryogenesis. 基于体细胞胚胎发生的桔梗高效基因编辑系统。

IF 9.5

园艺研究(英文) Pub Date : 2026-06-10 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag197

Chunlian Jin, Xingyu Zhou, Lingling Yuan, Fan Li, Jihua Wang

引用次数: 0

Extremely low light promotes adventitious shoot regeneration via RpPHYA/RpHYH-antagonized auxin biosynthesis and transport through dual regulation of RpPIN1 in Robinia pseudoacacia. 极弱光照通过RpPIN1的双重调控，通过rphya / rphyh拮抗生长素的生物合成和运输促进刺槐不定芽再生。

IF 9.5

园艺研究(英文) Pub Date : 2026-05-25 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag202

Juan Han, Yanting Tian, Jie Liu, Houyin Deng, Kunjin Han, Huayu Si, Shasha Ling, Shumin Zhang, Lixin Yu, Yousry A El-Kassaby, Yuhan Sun, Zuodeng Peng, Yun Li, Ye Zhao

{"title":"Extremely low light promotes adventitious shoot regeneration via RpPHYA/RpHYH-antagonized auxin biosynthesis and transport through dual regulation of RpPIN1 in Robinia pseudoacacia.","authors":"Juan Han, Yanting Tian, Jie Liu, Houyin Deng, Kunjin Han, Huayu Si, Shasha Ling, Shumin Zhang, Lixin Yu, Yousry A El-Kassaby, Yuhan Sun, Zuodeng Peng, Yun Li, Ye Zhao","doi":"10.1093/hr/uhag202","DOIUrl":"https://doi.org/10.1093/hr/uhag202","url":null,"abstract":"Extremely low light (ELL) is widely applied as a strategy to enhance adventitious shoot (AS) regeneration in woody plants. However, how ELL acts as an environmental signal to reprogram regenerative competence remains unclear. Here, we show that ELL for 14 days (ELL14) markedly promotes AS regeneration across multiple genotypes of Robinia pseudoacacia. Transcriptomic and functional enrichment analyses revealed that auxin biosynthesis, transport, and signaling constitute the core pathways activated under ELL14. Consistently, auxin levels increased, and the inhibition of biosynthesis or transport largely abolished the regeneration-promoting effect. Integrative weighted gene co-expression network analysis and regulatory analyses identified RpPHYA as a key integrator of ELL-responsive networks. Under ELL14, the RpPHYA-associated module promotes auxin biosynthesis by upregulating RpAMI1 and coordinates auxin transport through RpPIN1 via transcriptional regulation and RpROS1-mediated CHG demethylation, forming a dual regulatory mechanism. In contrast, under normal light, RpHYH antagonizes this regulatory program, thereby restricting regenerative capacity. Overexpression of RpPHYA, RpAMI1, RpPIN1, and RpROS1 significantly enhanced AS regeneration, functionally validating this ELL14-induced module. Collectively, our findings demonstrate that ELL functions as an environmental signal that promotes AS regeneration in R. pseudoacacia through coordinated transcriptional and DNA demethylation of auxin transport, providing mechanistic insights for improving regeneration in woody plants.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 10","pages":"uhag202"},"PeriodicalIF":9.5,"publicationDate":"2026-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits. 品种和栽培相关的果实花青素积累差异的多方面机制。

IF 9.5

园艺研究(英文) Pub Date : 2026-05-25 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag204

Ling Liang, Jiazhen Zhu, Yujie Yang, Shaojie Ai, Kui Lin-Wang, Andrew C Allan, Kunsong Chen, Changjie Xu

引用次数: 0

The MdERF1B-miR482c-MdCCR4-MdTLP1b module regulates apple resistance to Colletotrichum gloeosporioides. MdERF1B-miR482c-MdCCR4-MdTLP1b模块调控苹果对炭疽病的抗性。

IF 9.5

园艺研究(英文) Pub Date : 2026-05-21 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag199

Baodong Zhang, Jialin Cong, Zongshan Zhou

{"title":"The MdERF1B-miR482c-MdCCR4-MdTLP1b module regulates apple resistance to Colletotrichum gloeosporioides.","authors":"Baodong Zhang, Jialin Cong, Zongshan Zhou","doi":"10.1093/hr/uhag199","DOIUrl":"https://doi.org/10.1093/hr/uhag199","url":null,"abstract":"Glomerella leaf spot (GLS), incited by Colletotrichum gloeosporioides, poses a significant threat to apple (Malus domestica) production. microRNAs (miRNAs) are critical regulators of plant-pathogen crosstalk. However, the underlying mechanism through which miR482 regulates apple resistance to GLS remains unclear. Here, we show that overexpression of miR482c compromises resistance against C. gloeosporioides, while silencing miR482c confers resistance to C. gloeosporioides. 5'RACE assays in apple and co-expression assays in Nicotiana benthamiana confirmed that miR482c targets and cleaves the MdCCR4 mRNA. Subsequent research revealed that overexpression of MdCCR4 significantly enhances apple resistance to C. gloeosporioides. Notably, MdCCR4 physically interacts with the thaumatin-like protein MdTLP1b, which also positively regulates resistance to C. gloeosporioides. Additionally, MdERF1B was identified as an upstream regulator of miR482c through bioinformatics and Y1H screening, and was further confirmed to negatively regulate miR482c transcription by directly binding to its promoter. Meanwhile, MdERF1B overexpression enhances resistance to C. gloeosporioides, while silencing MdERF1B compromises resistance to C. gloeosporioides. Collectively, our findings indicate that the MdERF1B-miR482c-MdCCR4-MdTLP1b regulatory network is pivotal for apple defense against C. gloeosporioides, highlighting promising candidates for the genetic improvement of disease resistance.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 10","pages":"uhag199"},"PeriodicalIF":9.5,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13633095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tandem duplication underlies diversification of the gymnosperm-specific TPS-d subfamily. 串联复制是裸子植物特异性TPS-d亚家族多样化的基础。

IF 9.5

园艺研究(英文) Pub Date : 2026-05-20 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag195

Yuwei Liang, Xin Geng, Yijun Shen, Kuang Sheng, Xinyi Zhou, Danqing Li, Xiuyun Wang, Chuan Dong, Xiaofan Zhou, Liangsheng Zhang, Jiasheng Wu, Chengjun Zhang

引用次数: 0

Adaptive cold-environmental strategies of Lonicera caerulea: synergistic regulation of anthocyanin and iridoid pathways in cold tolerance and fruit ripening. 金银花的适应性冷环境策略：花青素和环烯醚酮途径在耐寒和果实成熟中的协同调节。

IF 9.5

园艺研究(英文) Pub Date : 2026-05-20 eCollection Date: 2026-10-01 DOI: 10.1093/hr/uhag198

Tianyi Wang, Qing Yang, Hongyan Cao, Tingting Du, Baoan Wang, Yuxin Fan, Cai Qin, Yadong Duan, Yujie Fu, Dong Meng

{"title":"Adaptive cold-environmental strategies of Lonicera caerulea: synergistic regulation of anthocyanin and iridoid pathways in cold tolerance and fruit ripening.","authors":"Tianyi Wang, Qing Yang, Hongyan Cao, Tingting Du, Baoan Wang, Yuxin Fan, Cai Qin, Yadong Duan, Yujie Fu, Dong Meng","doi":"10.1093/hr/uhag198","DOIUrl":"https://doi.org/10.1093/hr/uhag198","url":null,"abstract":"Understanding how plants adapt to extreme cold and ripen fruit is a key question in plant biology. Lonicera caerulea (blue honeysuckle), a cold-tolerant berry species native to the northern temperate zone, is valued as a 'noble fruit' for its rich nutrient profile and serves as an ideal model for studies due to its unique ability to thrive in low temperatures and ripen fruit during early spring. Here, we generated a chromosome-level genome assembly for wild L. caerulea from the Changbai Mountain region. We integrated it with transcriptomic and metabolomic analyses to explore its molecular cold adaptation mechanisms. We found that L. caerulea achieves cold tolerance and fruit ripening through dynamic regulation of two important metabolic pathways. Under cold stress, the LcODO1/LcMYB15-LcSLAS module promotes iridoid accumulation, while the LcbHLH62/LcMYB3R5-LcF3GT module enhances anthocyanin biosynthesis, jointly increasing plant cold resistance. During fruit maturation, metabolite profiles show dynamic reprogramming: anthocyanin levels rise in both pulp and peel, while the content of bitter iridoids markedly decreases in the pulp. This work provides a comprehensive genomic framework for understanding cold adaptation mechanisms, offering valuable gene resources and a theoretical foundation for improving cold resistance and fruit quality in breeding programs.","PeriodicalId":57479,"journal":{"name":"园艺研究(英文)","volume":"13 10","pages":"uhag198"},"PeriodicalIF":9.5,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149119256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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